Fatal acute Chagas disease by Trypanosoma cruzi DTU TcI, Ecuador

A radioactive Western-blotting technique was developed by which the reactivity of Immunoglobulins (Igs) from different classes to both membrane radiolabelled and internal parasite antigens is simultaneously identified. The method includes radioiodination of parasites, polypeptide fractionation by SDS-PAGE, Western-blot transfer and autoradiography of the immunoblots developed with anti-Igs conjugates labelled with enzymes. The analysis is then performed by the comparison of common bands on the autoradiograms and the respective substrate stained nitrocellulose blots. This technique was used to analyse T. cruzi trypomastigote surface labelled antigens reactive to IgM, IgA and IgG specific antibodies. A different pattern of reactivity with acute Chagas' disease patients sera was thus obtained.

Download Full-text

Treg Cells Induced by rSSP4 Derived fromT. cruziAmastigotes Increase Parasitemia in an Experimental Chagas Disease Model

BioMed Research International ◽

10.1155/2013/632436 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 8

Author(s):

Y. Flores-García ◽

J. L. Rosales-Encina ◽

V. H. Rosales-García ◽

A. R. Satoskar ◽

P. Talamás-Rohana

Keyword(s):

T Cells ◽

Trypanosoma Cruzi ◽

Chagas Disease ◽

Disease Model ◽

Treg Cells ◽

Immunosuppressive Activity ◽

Positive Role ◽

Cardiac Inflammation ◽

Considerable Controversy ◽

Acute Chagas Disease

Currently, there is a considerable controversy over the participation of Treg cells duringTrypanosoma cruziinfection, the main point being whether these cells play a negative or a positive role. In this work, we found that the adoptive transfer of CD4+CD25+FOXP3+T cells from rSSP4- (a recombinantTrypanosoma cruziamastigote derived protein, previously shown to have immunomodulatory properties on macrophages) immunized BALB/c donors into syngenic recipients simultaneously withT. cruzichallenge reduces cardiac inflammation and prolongs hosts’ survival but increases blood parasitemia and parasite loads in the heart. These CD4+CD25+FOXP3+Treg cells from immunized mice have a relatively TGF-β-dependent suppressive activity on CD4+T cells. Therefore, regulatory CD4+CD25+T cells play a positive role in the development of acuteT. cruziinfection by inducing immunosuppressive activity that controls early cardiac inflammation during acute Chagas disease, prolonging survival, but at the same time promoting parasite growth.

Download Full-text

NATURAL INFECTION BY Trypanosoma cruzi IN ONE DOG IN CENTRAL WESTERN BRAZIL: A CASE REPORT

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652013000400011 ◽

2013 ◽

Vol 55 (4) ◽

pp. 287-289 ◽

Cited By ~ 3

Author(s):

Arleana do Bom Parto Ferreira de Almeida ◽

Daphine Ariadne Jesus de Paula ◽

Maria Luisa Paro Otton ◽

Felipe Wolf Jaune ◽

Raquel Aparecida Sales da Cruz ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Chagas Disease ◽

Natural Infection ◽

Human Populations ◽

Direct Examination ◽

Chain Reaction ◽

Mato Grosso ◽

Blood Smears ◽

Polymerase Chain ◽

Acute Chagas Disease

SUMMARY It is estimated that about 10 million people are infected with Trypanosoma cruzi worldwide, mostly in Latin America and more than 25 million are at risk of acquiring this infection in endemic areas. Dogs are an important reservoir for this pathogen and thus, considered a risk factor for human populations. This report describes one case of Chagas disease in a dog from Cuiabá, Mato Grosso State, Brazil. The diagnosis was obtained by direct examination of trypomastigote forms in blood smears. Amastigotes forms were visualized in microscopy of the bone marrow, lymph nodes, kidneys, liver and brain. The T. cruzi (ZIII) infection was confirmed by Polymerase Chain Reaction, and sequencing. The animal presented multisystemic failure and died. Although acute Chagas disease in humans is not reported in Cuiabá, this is the first report of a canine case in this region. This case represents a warning, to health professionals and authorities, to the possibility of transmission of this zoonosis in Cuiabá.

Download Full-text

Trypanosoma cruzi defined antigens in the serological evaluation of an outbreak of acute Chagas disease in Brazil (Catolé do Rocha, Paraíba)

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02761996000100015 ◽

1996 ◽

Vol 91 (1) ◽

pp. 87-93 ◽

Cited By ~ 18

Author(s):

Eufrosina S Umezawa ◽

Maria Aparecida Shikanai-Yasuda ◽

Arthur Gruber ◽

Vera L Pereira-Chioccola ◽

Bianca Zingales

Keyword(s):

Trypanosoma Cruzi ◽

Chagas Disease ◽

Acute Chagas Disease

Download Full-text

Distantiae Transmission of Trypanosoma cruzi: A New Epidemiological Feature of Acute Chagas Disease in Brazil

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0002878 ◽

2014 ◽

Vol 8 (5) ◽

pp. e2878 ◽

Cited By ~ 40

Author(s):

Samanta Cristina das Chagas Xavier ◽

André Luiz Rodrigues Roque ◽

Daniele Bilac ◽

Vitor Antônio Louzada de Araújo ◽

Sócrates Fraga da Costa Neto ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Chagas Disease ◽

Acute Chagas Disease

Download Full-text

The trans-Sialidase from Trypanosoma cruzi Induces Thrombocytopenia during Acute Chagas' Disease by Reducing the Platelet Sialic Acid Contents

Infection and Immunity ◽

10.1128/iai.73.1.201-207.2005 ◽

2005 ◽

Vol 73 (1) ◽

pp. 201-207 ◽

Cited By ~ 71

Author(s):

María Virginia Tribulatti ◽

Juan Mucci ◽

Nico Van Rooijen ◽

María Susana Leguizamón ◽

Oscar Campetella

Keyword(s):

Sialic Acid ◽

Trypanosoma Cruzi ◽

Chagas Disease ◽

Virulence Factor ◽

Acid Content ◽

Fluorescent Protein ◽

Acute Infection ◽

Sialic Acid Content ◽

Platelet Surface ◽

Acute Chagas Disease

ABSTRACT Strong thrombocytopenia is observed during acute infection with Trypanosoma cruzi, the parasitic protozoan agent of American trypanosomiasis or Chagas' disease. The parasite sheds trans-sialidase, an enzyme able to mobilize the sialyl residues on cell surfaces, which is distributed in blood and is a virulence factor. Since the sialic acid content on the platelet surface is crucial for determining the half-life of platelets in blood, we examined the possible involvement of the parasite-derived enzyme in thrombocytopenia induction. We found that a single intravenous injection of trans-sialidase into naïve mice reduced the platelet count by 50%, a transient effect that lasted as long as the enzyme remained in the blood. CD43−/− mice were affected to a similar extent. When green fluorescent protein-expressing platelets were treated in vitro with trans-sialidase, their sialic acid content was reduced together with their life span, as determined after transfusion into naïve animals. No apparent deleterious effect on the bone marrow was observed. A central role for Kupffer cells in the clearance of trans-sialidase-altered platelets was revealed after phagocyte depletion by administration of clodronate-containing liposomes and splenectomy. Consistent with this, parasite strains known to exhibit more trans-sialidase activity induced heavier thrombocytopenia. Finally, the passive transfer of a trans-sialidase-neutralizing monoclonal antibody to infected animals prevented the clearance of transfused platelets. Results reported here strongly support the hypothesis that the trans-sialidase is the virulence factor that, after depleting the sialic acid content of platelets, induces the accelerated clearance of the platelets that leads to the thrombocytopenia observed during acute Chagas' disease.

Download Full-text

Possible oral transmission of acute Chagas' disease in Brazil

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46651991000500003 ◽

1991 ◽

Vol 33 (5) ◽

pp. 351-357 ◽

Cited By ~ 89

Author(s):

M.A. Shikanai-Yasuda ◽

C. Brisola Marcondes ◽

L. A. Guedes ◽

G.S. Siqueira ◽

A.A. Barone ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Chagas Disease ◽

Fatal Case ◽

Febrile Illness ◽

Epidemiological Survey ◽

High Rate ◽

Oral Transmission ◽

Chagas Cardiomyopathy ◽

Limb Edema ◽

Acute Chagas Disease

In October, 1986, 7 to 22 days after a meeting at a farm in Paraíba state, 26 individuals presented with a febrile illness associated with bilateral eyelid and lower limb edema, mild hepatosplenomegaly, lymphadenopathy and, occasionally a skin rash. A 11-year-old boy exhibited atrial premature complexes and a 74-year-old patient developed acute heart failure. In two patients hospitalized in São Paulo city, acute Chagas' disease was diagnosed by the demonstration of circulating Trypanosoma cruzi. At autopsy in a fatal case, acute Chagas' cardiomyopathy was demonstrated. Xenodiagnosis were positive in 9 out of 14 tested patients. A specific IgG immune response was found in all patients and specific IgM antibodies were identified in 20 out of 22 tested patients. A epidemiological survey showed the existence of Triatoma brasiliensis in the outbuildings of this farm, but none in the house where most of the guests stayed. A high rate of infection with Trypanosoma cruzi was found in opossums. These observations together with those related to the food consumed by the patients, lead the authors to suggest that the human infections resulted from oral contamination probably originating from naturally infected marsupials in the area or crushed infected bugs.

Download Full-text

Acute Chagas disease outbreak associated with oral transmission

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822008000300014 ◽

2008 ◽

Vol 41 (3) ◽

pp. 296-300 ◽

Cited By ~ 65

Author(s):

Juarez Pereira Dias ◽

Claudilson Bastos ◽

Eline Araújo ◽

Ana Verônica Mascarenhas ◽

Eduardo Martins Netto ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Chagas Disease ◽

Disease Outbreak ◽

Serological Tests ◽

Oral Transmission ◽

Triatoma Sordida ◽

Acute Chagas Disease

Seven individuals living in a town in the Southwest of Bahia developed sudden signs of cardiac and systemic impairment, with lethality of 28.6%. Serological tests were positive at least in one test in the five patients examined. Forty percent of the Triatoma sordida mynphs found inside or around Trypanosoma cruzi were found by blood culturig in there out five cases the homes of these cases were positive for Trypanosoma cruzi. Transmission probably occurred through consumption of water contaminated with triatomine feces. These findings emphasize the necessity to evaluation the importance of vectors like Triatoma sordida in maintaining the endemicity of this disease.

Download Full-text