scholarly journals SIRT1 (rs3740051) role in pituitary adenoma development

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Rasa Liutkeviciene ◽  
Alvita Vilkeviciute ◽  
Greta Morkunaite ◽  
Brigita Glebauskiene ◽  
Loresa Kriauciuniene
Keyword(s):  
Polymerase Chain Reaction ◽  
Pituitary Adenoma ◽  
Polymerase Chain Reaction Method ◽  
Control Group ◽  
Study Group ◽  
Chain Reaction ◽  
Lower Odds ◽  
Reaction Method ◽  
Non Invasive ◽  
Polymerase Chain

Abstract Background Our purpose was to determine if SIRT1 (rs4746720, rs3740051) genotypes have an influence on the development of pituitary adenoma (PA). Methods The study group included 142 patients with pituitary adenoma (PA) and the control group consisted of 826 healthy people. The genotyping of SIRT1 (rs4746720, rs3740051) was carried out using the real-time polymerase chain reaction method. Results Statistically significant results were obtained in the analysis of SIRT1 rs3740051. Significant differences in genotype (G/G, G/A, A/A) distribution were obtained comparing patients with PA without recurrence and PA with recurrence (0, 17.9, 82.1% vs. 6.7, 6.7, 86.7%, respectively, p = 0.022). Also, statistically significant differences were observed when comparing the genotype (G/G, G/A, A/A) distribution in the non-invasive PA group and the invasive PA group (3.4, 25.9, 70.7% vs. 0, 8.3, 91.7%, respectively, p = 0.003), and allele G was less frequently observed in invasive PA, than in non-invasive PA (4.2% vs. 16.4%, p < 0,001). Further analysis revealed that G/A (OR = 0.261; 95% CI:0.099–0.689; p = 0.007) and each allele A (OR = 0.229; 95% CI:0.091–0.575; p = 0.002) were associated with lower odds of occurring an invasive PA. Conclusions Our study revealed that SIRT1 rs3740051 is associated with PA recurrence and invasiveness. The haplotype containing alleles C-A in rs12778366-rs3740051 was found to be associated with increased odds of PA development as well.

scholarly journals Does MMP-9 Gene Polymorphism Play a Role in Pituitary Adenoma Development?

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Brigita Glebauskiene ◽  
Rasa Liutkeviciene ◽  
Alvita Vilkeviciute ◽  
Loresa Kriauciuniene ◽  
Silvija Jakstiene ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pituitary Adenoma ◽  
Gene Polymorphism ◽  
Reference Group ◽  
Polymerase Chain Reaction Method ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Reaction Method ◽  
Healthy Control ◽  
Polymerase Chain

Purpose. To determine if the MMP-9 genotype has an influence on development of pituitary adenoma (PA). Methodology. The study enrolled n=86 patients with PA and n=526 healthy controls (reference group). The genotyping of MMP-9 was carried out using the real-time polymerase chain reaction method. Results. Our data demonstrated that the MMP-9 (–1562) C/C genotype was more frequent in PA group than in healthy controls (81.4% versus 64.6%, p=0.002); C/C genotype was more frequently present in PA females compared to healthy control females, 81.5% versus 64.6%, p=0.018, as well. MMP-9 (–1562) C/C genotype was frequently observed for all subgroups: noninvasive and invasive, nonrecurrence, and inactive PA compared to healthy controls: 81.8% versus 64.6%, p=0.021; 81.0% versus 64.6%, p=0.041; 81.8% versus 64.6%, p=0.005; 100.0% versus 64.6%, p<0.001, respectively. MMP-9 (–1562) C/C genotype was more frequent in inactive PA compared to active PA: 100.0% versus 71.4%; p<0.001. Conclusion. MMP-9 (–1562) C/C genotype plays a role in nonrecurrence, inactive, and invasive as well as in nonivasive PA development.

scholarly journals Role of MMP-2 (-1306 C/T) Polymorphism in Pituitary Adenoma

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Brigita Glebauskiene ◽  
Rasa Liutkeviciene ◽  
Alvita Vilkeviciute ◽  
Loresa Kriauciuniene ◽  
Giedrimantas Bernotas ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pituitary Adenoma ◽  
Gene Polymorphism ◽  
Reference Group ◽  
Polymerase Chain Reaction Method ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

Purpose. To determine if the frequency of the genotype ofMMP-2 (-1306 C/T) Rs243865has an influence on the development of pituitary adenoma (PA).Methods. The study enrolledn=84patients with PA and a random sample of the populationn=318(reference group). The genotyping test ofMMP-2 (-1306 C/T)was carried out using the real-time polymerase chain reaction method.Results. Analysis ofMMP-2 (-1306 C/T)gene polymorphism has not revealed any differences in the genotype (C/C, C/T,andT/T) distribution between the PA patients and the reference group (as follows: 50%, 44%, and 6% versus 59.75%, 33.96%, and 6.29%).MMP-2 (-1306) C/Cgenotype was rarely observed in noninvasive PA compared to healthy controls: 35.1% versus 59.75%;p=0.0049, as well C/C genotype being more frequently detected in nonrecurrence PA compared to healthy controls: 46.5% versus 59.75%;p=0.0468.MMP-2 (-1306) C/Tgenotype was more frequently present in PA females compared to healthy controls females: 49.1% versus 33.66%;p=0.041.Conclusion. Patients with noninvasive and nonrecurrence pituitary adenoma were the carriers of the C/C genotype significantly more frequently than their control counterparts and the C/T genotype in females was more frequent.

scholarly journals INFLUENCE OF LYS198ASN POLYMORPHISM OF ENDOTHELIN-1 GENE ON ISCHEMIC ATHEROTHROMBOTIC STROKE CHARACTERISTICS

2020 ◽  
Vol 73 (4) ◽  
pp. 657-661
Author(s):  
Tetiana B. Oleshko ◽  
Iryna S. Chaika ◽  
Tetiana M. Oleshko ◽  
Viktoriia Yu. Harbuzova
Keyword(s):  
Polymerase Chain Reaction ◽  
Venous Blood ◽  
Polymerase Chain Reaction Method ◽  
Control Group ◽  
Chain Reaction ◽  
Reaction Method ◽  
Major Allele ◽  
Polymerase Chain ◽  
Length Analysis ◽  
Restriction Fragment Length

The aim: Investigate the effect of Lys198Asn polymorphism of the EDN1 gene on ischemic atherothrombotic stroke characteristics. Materials and methods: Venous blood of 170 patients with ischemic atherothrombotic stroke (IAS) and 124 patients without cerebrovascular pathology, who made up the control group, used for the study. Lys198Asn (rs5370) polymorphism of the EDN1 gene was determined by the polymerase chain reaction method followed by restriction fragment length analysis. Statistical analysis was performed using SPSS-17.0. The values of Р < 0.05 were considered reliable. Results: An association between the Lys198Asn polymorphism of the EDN1 gene and the IAS development was detected. For Asn/Asn genotype carriers, the risk of IAS developing is 4 times higher than that of homozygotes for the major allele. The association of this polymorphism with the arterial pool, whose atherothrombotic changes lead to the development of IAS, was found in individuals with BMI < 25 kg/m2. Lys198Asn polymorphism also affects the severity of IAS in persons with hypertension and non-smokers. Conclusion: The Lys198Asn polymorphism of the EDN1 gene influences some characteristics of ischemic stroke.

scholarly journals Clinical and Microbiological Evaluation of Biofilm-Gingival Interface Classification in Patients with Generalized Forms of Periodontitis

2014 ◽  
Vol 63 (2) ◽  
pp. 175-181 ◽  
Author(s):  
JAN KOWALSKI ◽  
RENATA GÓRSKA
Keyword(s):  
Polymerase Chain Reaction ◽  
Periodontal Diseases ◽  
Polymerase Chain Reaction Method ◽  
Study Group ◽  
Subgingival Plaque ◽  
Chain Reaction ◽  
Study Objective ◽  
Reaction Method ◽  
Group A ◽  
Polymerase Chain

STUDY OBJECTIVE: In 2008 a new division of periodontal diseases based on the concept of biofilm-gingival interface (BGI), was presented. The aim of this study was to assess whether the proposed BGI categories could be useful in diagnosing patients with generalized forms of both aggressive (gAP) and chronic (gCP) periodontitis. DESIGN AND KEY METHODS: The study group consisted of 40 individuals (17 with gAP and 23 with gCP). All patients were divided into three groups according to the BGI scale: 4 patients with P1 (pockets deeper than 4 mms with bleeding index below 10%), 20 individuals with P2 (pockets deeper than 4 mms with bleeding index 10-50%) and 16 individuals with P3 (pockets deeper than 4 mms with bleeding index above 50%). The presence of bacteria in subgingival plaque was analyzed using the polymerase chain reaction method. RESULTS: Statistically significant differences with respect to all parameters measured were found when patients were grouped according to the BGI scale--they were higher in P3 group. In gAP group A. actinomycetemcomitans and C. rectus were found in a higher numbers. No such differences were observed between P2 and P3 groups. CONCLUSIONS: The BGI scale seems to be helpful in effective and quick classifying of patients into adequate clinical subgroups.

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