Role of MMP-2 (-1306 C/T) Polymorphism in Pituitary Adenoma

Purpose. To determine if the frequency of the genotype ofMMP-2 (-1306 C/T) Rs243865has an influence on the development of pituitary adenoma (PA).Methods. The study enrolledn=84patients with PA and a random sample of the populationn=318(reference group). The genotyping test ofMMP-2 (-1306 C/T)was carried out using the real-time polymerase chain reaction method.Results. Analysis ofMMP-2 (-1306 C/T)gene polymorphism has not revealed any differences in the genotype (C/C, C/T,andT/T) distribution between the PA patients and the reference group (as follows: 50%, 44%, and 6% versus 59.75%, 33.96%, and 6.29%).MMP-2 (-1306) C/Cgenotype was rarely observed in noninvasive PA compared to healthy controls: 35.1% versus 59.75%;p=0.0049, as well C/C genotype being more frequently detected in nonrecurrence PA compared to healthy controls: 46.5% versus 59.75%;p=0.0468.MMP-2 (-1306) C/Tgenotype was more frequently present in PA females compared to healthy controls females: 49.1% versus 33.66%;p=0.041.Conclusion. Patients with noninvasive and nonrecurrence pituitary adenoma were the carriers of the C/C genotype significantly more frequently than their control counterparts and the C/T genotype in females was more frequent.

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Does MMP-9 Gene Polymorphism Play a Role in Pituitary Adenoma Development?

Disease Markers ◽

10.1155/2017/5839528 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 5

Author(s):

Brigita Glebauskiene ◽

Rasa Liutkeviciene ◽

Alvita Vilkeviciute ◽

Loresa Kriauciuniene ◽

Silvija Jakstiene ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Pituitary Adenoma ◽

Gene Polymorphism ◽

Reference Group ◽

Polymerase Chain Reaction Method ◽

Healthy Controls ◽

Chain Reaction ◽

Reaction Method ◽

Healthy Control ◽

Polymerase Chain

Purpose. To determine if the MMP-9 genotype has an influence on development of pituitary adenoma (PA). Methodology. The study enrolled n=86 patients with PA and n=526 healthy controls (reference group). The genotyping of MMP-9 was carried out using the real-time polymerase chain reaction method. Results. Our data demonstrated that the MMP-9 (–1562) C/C genotype was more frequent in PA group than in healthy controls (81.4% versus 64.6%, p=0.002); C/C genotype was more frequently present in PA females compared to healthy control females, 81.5% versus 64.6%, p=0.018, as well. MMP-9 (–1562) C/C genotype was frequently observed for all subgroups: noninvasive and invasive, nonrecurrence, and inactive PA compared to healthy controls: 81.8% versus 64.6%, p=0.021; 81.0% versus 64.6%, p=0.041; 81.8% versus 64.6%, p=0.005; 100.0% versus 64.6%, p<0.001, respectively. MMP-9 (–1562) C/C genotype was more frequent in inactive PA compared to active PA: 100.0% versus 71.4%; p<0.001. Conclusion. MMP-9 (–1562) C/C genotype plays a role in nonrecurrence, inactive, and invasive as well as in nonivasive PA development.

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Study of the HLA-DPβ1 Locus by the Polymerase Chain Reaction Technique in Patients with Hodgkin's Disease

Tumori Journal ◽

10.1177/030089169307900211 ◽

1993 ◽

Vol 79 (2) ◽

pp. 133-136 ◽

Cited By ~ 2

Author(s):

Guseppe Pellegris ◽

Claudia Lombardo ◽

Annelisa Cantoni ◽

Liliana Devizzi ◽

Monica Balzarotti

Keyword(s):

Polymerase Chain Reaction ◽

Hodgkin's Disease ◽

Hodgkin’S Disease ◽

Polymerase Chain Reaction Method ◽

Healthy Controls ◽

Chain Reaction ◽

Reaction Method ◽

Significant Difference ◽

Polymerase Chain

Background A number of reports have studied associations between Hodgkin's disease and HLA. Some of them established correlation between several antigens and Hodgkin's disease, and others found no correlations. Methods The HLA DP locus was determined by the polymerase chain reaction method in 31 Hodgkin's disease patients and 58 healthy controls. Results No significant difference between patients and controls was noted. Conclusions Further investigations are needed to confirm the hypothesis of a possible role of the HLA complex as one of the factors involved in Hodgkin's disease.

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Low frequency of CYP2A6 gene polymorphism as revealed by a one-step polymerase chain reaction method

Pharmacogenetics ◽

10.1097/00008571-199906000-00007 ◽

1999 ◽

Vol 9 (3) ◽

pp. 327-332 ◽

Cited By ~ 37

Author(s):

Gen-Fu Chen ◽

Yong-Ming Tang ◽

Bridgett Green ◽

Dong-Xin Lin ◽

F. Peter Guengerich ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Gene Polymorphism ◽

Low Frequency ◽

Polymerase Chain Reaction Method ◽

Chain Reaction ◽

Reaction Method ◽

One Step ◽

Polymerase Chain ◽

Cyp2a6 Gene ◽

Step Polymerase Chain Reaction

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Role of type I interferon in the bacillus Calmette-Guérin-induced expression of CXCL10 from human monocytes

Mediators of Inflammation ◽

10.1080/09629350400014099 ◽

2004 ◽

Vol 13 (5-6) ◽

pp. 343-348 ◽

Cited By ~ 10

Author(s):

Patricia Méndez Samperio ◽

Artemisa Trejo ◽

Elena Miranda

Keyword(s):

Polymerase Chain Reaction ◽

Polymerase Chain Reaction Method ◽

Type I ◽

Mrna Levels ◽

Human Monocytes ◽

Bacillus Calmette Guerin ◽

Chain Reaction ◽

Reaction Method ◽

Polymerase Chain

BACKGROUND: The proinflammatory chemokine CXCL10, in addition to its chemotactic properties, is also involved in the stimulation of natural killer and T-cell migration inMycobacterium tuberculosisinfection. In this study, our experiments were designed to determine the role of interferon (IFN)-αβ in the production of CXCL10 by human monocytes infected withMycobacterium bovisbacillus Calmette-Guérin (BCG).Methods: The concentrations of CXCL10 in culture supernatants of monocytes infected withM. bovisBCG were determined by enzyme-linked immunosorbent assay. CXCL10 mRNA levels were determined by the reverse transcription-polymerase chain reaction method.Results: We have shown the induction of CXCL10 following infection withM. bovisBCG in a dose-dependent and time-dependent manner. Importantly, the secretion of CXCL10 in response toM. boviswas increased by IFN-α. These results were further confirmed by the fact that the addition of an anti-IFN-αβ neutralizing antibody completely reversed the stimulatory effect, whereas an isotype-matched control antibody had no significant effect on CXCL10 secretion. It is important to note that no significant effect of type I IFN on CXCL8 production inM. bovis-infected monocytes was observed. This was consistent with the finding by the reverse transcription-polymerase chain reaction method that treatment with anti-IFN-α/β antibodies potentially inhibited CXCL10 mRNA levels, whereas no significant effect was observed on CXCL8 mRNA. Moreover, in THP-1 monocytes and THP-1 macrophages, the addition of exogenous IFN-α stimulated CXCL10 secretion.Conclusions: Collectively, these results indicate that the type I IFN may play an important role to modulate the expression of CXCL10 inM. bovisBCG infection. Studies onM. bovis-induced chemokine secretion could provide important insight into the regulation of the immune response against tuberculosis.

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SIRT1 (rs3740051) role in pituitary adenoma development

BMC Medical Genetics ◽

10.1186/s12881-019-0892-x ◽

2019 ◽

Vol 20 (1) ◽

Author(s):

Rasa Liutkeviciene ◽

Alvita Vilkeviciute ◽

Greta Morkunaite ◽

Brigita Glebauskiene ◽

Loresa Kriauciuniene

Keyword(s):

Polymerase Chain Reaction ◽

Pituitary Adenoma ◽

Polymerase Chain Reaction Method ◽

Control Group ◽

Study Group ◽

Chain Reaction ◽

Lower Odds ◽

Reaction Method ◽

Non Invasive ◽

Polymerase Chain

Abstract Background Our purpose was to determine if SIRT1 (rs4746720, rs3740051) genotypes have an influence on the development of pituitary adenoma (PA). Methods The study group included 142 patients with pituitary adenoma (PA) and the control group consisted of 826 healthy people. The genotyping of SIRT1 (rs4746720, rs3740051) was carried out using the real-time polymerase chain reaction method. Results Statistically significant results were obtained in the analysis of SIRT1 rs3740051. Significant differences in genotype (G/G, G/A, A/A) distribution were obtained comparing patients with PA without recurrence and PA with recurrence (0, 17.9, 82.1% vs. 6.7, 6.7, 86.7%, respectively, p = 0.022). Also, statistically significant differences were observed when comparing the genotype (G/G, G/A, A/A) distribution in the non-invasive PA group and the invasive PA group (3.4, 25.9, 70.7% vs. 0, 8.3, 91.7%, respectively, p = 0.003), and allele G was less frequently observed in invasive PA, than in non-invasive PA (4.2% vs. 16.4%, p < 0,001). Further analysis revealed that G/A (OR = 0.261; 95% CI:0.099–0.689; p = 0.007) and each allele A (OR = 0.229; 95% CI:0.091–0.575; p = 0.002) were associated with lower odds of occurring an invasive PA. Conclusions Our study revealed that SIRT1 rs3740051 is associated with PA recurrence and invasiveness. The haplotype containing alleles C-A in rs12778366-rs3740051 was found to be associated with increased odds of PA development as well.

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