scholarly journals Effect of SMILE-derived decellularized lenticules as an adhesion barrier in a rabbit model of glaucoma filtration surgery

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Houfa Yin ◽  
Xinyi Chen ◽  
Xiaogang Hong ◽  
Jian Ma ◽  
Fang Wu ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Adjunctive Treatment ◽  
Control Group ◽  
Small Incision Lenticule Extraction ◽  
Filtration Surgery ◽  
Glaucoma Filtration Surgery ◽  
Adhesion Barrier ◽  
Adverse Side Effects ◽  
Subconjunctival Space ◽  
Lenticule Extraction

Abstract Background To investigate the effects of small incision lenticule extraction (SMILE)-derived decellularized lenticules on intraocular pressure (IOP) and conjunctival scarring in a rabbit model of glaucoma filtration surgery. Methods Trabeculectomy was performed on both eyes of New Zealand rabbits. A decellularized lenticule was placed in the subconjunctival space in one eye of the rabbits (the decellularized lenticule group), and no adjunctive treatment was performed in the fellow eye (the control group). The filtering bleb features and IOP were evaluated 0, 3, 7, 14, 21, and 28 days after surgery, and histopathologic examination was performed 28 days after surgery. Results Decellularized lenticules significantly increased bleb survival and decreased IOP postoperatively in the rabbit model with no adverse side effects. The histopathologic results showed a larger subconjunctival space and less subconjunctival fibrosis in the decellularized lenticule group. Conclusions Decellularized lenticules can prevent postoperative conjunctiva-sclera adhesion and fibrosis, and they may represent a novel antifibrotic agent for trabeculectomy.

Effects Of SMILE-Derived Decellularized Lenticule As An Adhesion Barrier In A Rabbit Model of Glaucoma Filtration Surgery

2021 ◽  
Author(s):  
Houfa Yin ◽  
Xinyi Chen ◽  
Xiaogang Hong ◽  
Jian Ma ◽  
Fang Wu ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Adjunctive Treatment ◽  
Control Group ◽  
Small Incision Lenticule Extraction ◽  
Filtration Surgery ◽  
Glaucoma Filtration Surgery ◽  
Adhesion Barrier ◽  
Adverse Side Effects ◽  
Subconjunctival Space ◽  
Lenticule Extraction

Abstract Background: To investigate the effects of small incision lenticule extraction (SMILE)-derived decellularized lenticule on intraocular pressure (IOP) and conjunctival scarring in a rabbit model of glaucoma filtration surgery. Methods: Trabeculectomy was performed on both eyes of New Zealand rabbits. Decellularized lenticule was placed in the subconjunctival space in one eye of the rabbits (decellularized lenticule group), and no adjunctive treatment was performed in the fellow eye (control group). The filtering bleb features and IOP were evaluated 0, 3, 7, 14, 21, and 28 days after surgery, and histopathologic examination was performed 28 days after surgery. Results: Decellularized lenticule significantly increased bleb survival and decreased IOP postoperatively in the rabbit model with no adverse side effects. Histopathologic results showed a larger subconjunctival space and less subconjunctival fibrosis in the decellularized lenticule group. Conclusions: Decellularized lenticule can prevent postoperative conjunctiva-sclera adhesion and fibrosis, and it may represent a novel antifibrotic agent for trabeculectomy.

scholarly journals Pluripotent epigenetic regulator OBP-801 maintains filtering blebs in glaucoma filtration surgery model

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuji Yamamoto ◽  
Atsushi Mukai ◽  
Toru Ikushima ◽  
Yasuo Urata ◽  
Shigeru Kinoshita ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rabbit Model ◽  
Vehicle Control ◽  
Control Group ◽  
Eye Drops ◽  
Subconjunctival Injection ◽  
Filtration Surgery ◽  
Glaucoma Filtration Surgery ◽  
Vehicle Control Group ◽  
Epigenetic Regulator

AbstractInhibition of fibrosis is indispensable for maintaining filtering blebs after glaucoma filtration surgery (GFS). The purpose of this study was to investigate the ability of a pluripotent epigenetic regulator OBP-801 (OBP) to ameliorate extracellular matrix formation in a rabbit model of GFS. Rabbits that underwent GFS were treated with OBP. The gene expression profiles and intraocular pressure (IOP) were monitored until 30 postoperative days. The bleb tissues were evaluated for tissue fibrosis at 30 postoperative days. In in vitro models, OBP interfered the functions of diverse genes during the wound-healing process. In in vivo GFS models, the expressions of TGF-β3, MMP-2, TIMP-2 and 3, LOX, COL1A and SERPINH1 were significantly inhibited at 30 postoperative days in the OBP group compared with those in the vehicle control group. OBP treatment involving subconjunctival injection or eye drops showed no adverse effects, and reduced levels of α-SMA and collagen deposition at the surgical wound site. OBP maintained the long-lived bleb without scar formation, and IOP was lower at 30 postoperative days compared with the vehicle control group. These findings suggest that OBP is an effective and useful candidate low-molecular-weight agent for improving wound healing and surgical outcomes in a rabbit model of GFS.

scholarly journals A Thin Honeycomb-patterned Film as an Adhesion Barrier in an Animal Model of Glaucoma Filtration Surgery

2009 ◽  
Vol 18 (3) ◽  
pp. 220-226 ◽  
Author(s):  
Tetsuhiko Okuda ◽  
Tomomi Higashide ◽  
Yukako Fukuhira ◽  
Yoshihiko Sumi ◽  
Masatsugu Shimomura ◽  
...  
Keyword(s):  
Animal Model ◽  
Filtration Surgery ◽  
Glaucoma Filtration Surgery ◽  
Adhesion Barrier

scholarly journals Sequential Therapy with Saratin, Bevacizumab and Ilomastat to Prolong Bleb Function following Glaucoma Filtration Surgery in a Rabbit Model

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138054 ◽  
Author(s):  
Gina M. Martorana ◽  
Jamie L. Schaefer ◽  
Monica A. Levine ◽  
Zachary L. Lukowski ◽  
Jeff Min ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Sequential Therapy ◽  
Filtration Surgery ◽  
Glaucoma Filtration Surgery

scholarly journals Risk Factors and Incidence of Suction Loss during Small Incision Lenticule Extraction (SMILE) in 8493 eyes

2020 ◽  
Author(s):  
Tian-Ze Huang ◽  
Ling Shen ◽  
Xiao-Ning Yu ◽  
Hong-Ying Jin
Keyword(s):  
Risk Factors ◽  
Eye Movements ◽  
Significant Risk ◽  
Male Gender ◽  
Control Group ◽  
Small Incision Lenticule Extraction ◽  
Patient Anxiety ◽  
Binary Logistic Regression Model ◽  
Small Incision ◽  
Lenticule Extraction

Abstract Background: To report the incidence and risk factors of suction loss during small incision lenticule extraction (SMILE).Methods: This retrospective comparative case control study included 8493 eyes of 4261 patients. Patients underwent SMILE surgery between January 2014 and September 2019 were included. Videos of suction loss were reviewed, and the direct causes of suction loss were noted. An independent samples t-test was used for comparisons between the suction loss group and the control group. A binary logistic regression model was used to determine the possible significant risk factors that might increase the likelihood of suction loss during SMILE surgery.Results: Suction loss occurred in 31 (0.37%) eyes of 30 patients; 23 (74.2%) cases occurred in the right eye (the first operative eye) and 8 (25.8%) cases occurred in the left eye. Among the 30 patients, 23 (76.7%) were male and 7 (23.3%) were female. The incidence in the six consecutive years were 0%, 2.13%, 0.34%, 0.24%, 0.22%, and 0.25%. Head and eye movements during surgery caused suction loss in 16 (51.6%) and 15 (48.4%) eyes, respectively. Comparison between the suction loss group and the control group showed that the first operative eye and male gender are at a significantly high risk for suction loss (p < 0.05).Conclusions: The risk factors of suction loss were first operative eye and male gender. Head and eye movements due to patient anxiety are the most common direct causes of suction loss. Surgeon’s experience may help to reduce the incidence of suction loss. Preoperative education and better communication during surgery needs to be emphasized.Trial registration: Retrospectively registered. ChiCTR-ORC-17011040. Registered 1 April 2017. Name of registry: The observation of clinical results after corneal refractive surgery. Data of enrollment of the first participant to the trial: 1 January 2014.

scholarly journals AR12286 Alleviates TGF-β-Related Myofibroblast Transdifferentiation and Reduces Fibrosis after Glaucoma Filtration Surgery

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4422
Author(s):  
Wen-Sheng Cheng ◽  
Ching-Long Chen ◽  
Jiann-Torng Chen ◽  
Le-Tien Lin ◽  
Shu-I Pao ◽  
...  
Keyword(s):  
Cell Migration ◽  
Intraocular Pressure ◽  
Kinase Inhibitor ◽  
Control Group ◽  
Filtration Surgery ◽  
Glaucoma Filtration Surgery ◽  
Collagen Contraction ◽  
Β Receptor ◽  
Tgf Β1

Scar formation can cause the failure of glaucoma filtration surgery. We investigated the effect of AR12286, a selective Rho-associated kinase inhibitor, on myofibroblast transdifferentiation and intraocular pressure assessment in rabbit glaucoma filtration surgery models. Cell migration and collagen contraction were used to demonstrate the functionality of AR12286-modulated human conjunctival fibroblasts (HConFs). Polymerase chain reaction quantitative analysis was used to determine the effect of AR12286 on the production of collagen Type 1A1 and fibronectin 1. Cell migration and collagen contraction in HConFs were activated by TGF-β1. However, compared with the control group, rabbit models treated with AR12286 exhibited higher reduction in intraocular pressure after filtration surgery, and decreased collagen levels at the wound site in vivo. Therefore, increased α-SMA expression in HConFs induced by TGF-β1 could be inhibited by AR12286, and the production of Type 1A1 collagen and fibronectin 1 in TGF-β1-treated HConFs was inhibited by AR12286. Overall, the stimulation of HConFs by TGF-β1 was alleviated by AR12286, and this effect was mediated by the downregulation of TGF-β receptor-related SMAD signaling pathways. In vivo results indicated that AR12286 thus improves the outcome of filtration surgery as a result of its antifibrotic action in the bleb tissue because AR12286 inhibited the TGF-β receptor-related signaling pathway, suppressing several downstream reactions in myofibroblast transdifferentiation.

scholarly journals Two-year observation of posterior corneal elevations after small incision lenticule extraction (SMILE) for myopia higher than −10 dioptres

2019 ◽  
Vol 104 (1) ◽  
pp. 142-148 ◽  
Author(s):  
Xueyi Zhou ◽  
Jianmin Shang ◽  
Bing Qin ◽  
Yu Zhao ◽  
Xingtao Zhou
Keyword(s):  
Central Area ◽  
Control Group ◽  
Small Incision Lenticule Extraction ◽  
Small Incision ◽  
Long Term Follow Up ◽  
Lenticule Extraction ◽  
Measured Change

AimTo investigate the change in posterior corneal elevations (PCEs) of eyes with extremely high myopia 2 years after small incision lenticule extraction (SMILE).MethodsWe evaluated 39 eyes of 39 patients with spherical equivalent higher than −10.00 dioptres (D). Using a Scheimpflug camera (Pentacam), we measured change in PCEs at 1 day, 3 months, 6 months and 2 years after SMILE. Another 34 eyes of 34 patients who underwent femtosecond laser-assisted in situ keratomileusis (FS-LASIK) were examined before, at 1 day and long-term after surgery as the control group. For each eye, elevations at central, thinnest, maximal points and 24 other predetermined points were measured.ResultsNo significant forward displacements of PCEs were observed in both surgeries. The maximal but not significant forward displacement occurred around 3–6 months following SMILE, and all returned to original levels 6 months postoperatively except superior area. The peripheral area tended to displace backward, while the central area tended forwardly. In both procedures, elevations along horizontal meridians, inferior and temporal hemispheres were significantly higher than those along vertical meridians, superior and nasal hemispheres, respectively (p<0.05). Elevation on the 4 mm, 6 mm diameters at 1 day and on the 6 mm diameter and temporal hemisphere at long-term follow-up postoperatively were significantly higher in FS-LASIK than SMILE (p<0.05). Change in elevations on the 6 mm diameter circle correlated with residual bed thickness (p=0.047).ConclusionsSMILE is a safe way to correct for myopia higher than −10 D, with PCEs remaining stable 2 years after surgery.

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