The emerging role of microRNAs and long noncoding RNAs in drug resistance of hepatocellular carcinoma

Abstract Hepatocellular carcinoma (HCC) is the fifth most common malignancy worldwide and the second most lethal human cancer. A portion of patients with advanced HCC can significantly benefit from treatments with sorafenib, adriamycin, 5-fluorouracil and platinum drugs. However, most HCC patients eventually develop drug resistance, resulting in a poor prognosis. The mechanisms involved in HCC drug resistance are complex and inconclusive. Human transcripts without protein-coding potential are known as noncoding RNAs (ncRNAs), including microRNAs (miRNAs), small nucleolar RNAs (snoRNAs), long noncoding RNAs (lncRNAs) and circular RNA (circRNA). Accumulated evidences demonstrate that several deregulated miRNAs and lncRNAs are important regulators in the development of HCC drug resistance which elucidates their potential clinical implications. In this review, we summarized the detailed mechanisms by which miRNAs and lncRNAs affect HCC drug resistance. Multiple tumor-specific miRNAs and lncRNAs may serve as novel therapeutic targets and prognostic biomarkers for HCC.

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Long Noncoding RNA Plays a Key Role in Metastasis and Prognosis of Hepatocellular Carcinoma

BioMed Research International ◽

10.1155/2014/780521 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 23

Author(s):

Guangbing Li ◽

Haohai Zhang ◽

Xueshuai Wan ◽

Xiaobo Yang ◽

Chengpei Zhu ◽

...

Keyword(s):

Gene Expression ◽

Hepatocellular Carcinoma ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Regulation Of Gene Expression ◽

Noncoding Rnas ◽

Long Noncoding Rnas ◽

Comprehensive Review ◽

Cellular Processes

Long noncoding RNAs (lncRNAs) have been attracting immense research interests. However, only a handful of lncRNAs had been thoroughly characterized. They were involved in fundamental cellular processes including regulation of gene expression at epigenetics as well as tumorogenesis. In this paper, we give a systematic and comprehensive review of existing literature about lncRNA involvement in hepatocellular carcinoma. This review exhibited that lncRNAs played important roles in tumorigenesis and subsequent prognosis and metastasis of hepatocellular carcinoma and elucidated the role of some specific lncRNAs such as MALAT1 and HOTAIR in the pathophysiology of hepatocellular carcinoma and their potential of being therapeutic targets.

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The Regulatory Role of Long Noncoding RNAs in Cancer Drug Resistance

Methods in Molecular Biology - Cancer Drug Resistance ◽

10.1007/978-1-4939-3347-1_12 ◽

2016 ◽

pp. 207-227 ◽

Cited By ~ 12

Author(s):

Marjan E. Askarian-Amiri ◽

Euphemia Leung ◽

Graeme Finlay ◽

Bruce C. Baguley

Keyword(s):

Drug Resistance ◽

Noncoding Rnas ◽

Long Noncoding Rnas ◽

Regulatory Role ◽

Cancer Drug ◽

Cancer Drug Resistance

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Long Noncoding RNAs as a Key Player in Hepatocellular Carcinoma

Biomarkers in Cancer ◽

10.1177/1179299x17737301 ◽

2017 ◽

Vol 9 ◽

pp. 1179299X1773730 ◽

Cited By ~ 22

Author(s):

Mrigaya Mehra ◽

Ranjit Chauhan

Keyword(s):

Hepatocellular Carcinoma ◽

Molecular Mechanisms ◽

Structural Integrity ◽

Gene Expression Regulation ◽

Noncoding Rnas ◽

Clinical Applications ◽

Long Noncoding Rnas ◽

Protein Coding ◽

Cellular Processes ◽

The World

Hepatocellular carcinoma (HCC) is a major malignancy in the liver and has emerged as one of the main cancers in the world with a high mortality rate. However, the molecular mechanisms of HCC are still poorly understood. Long noncoding RNAs (lncRNAs) have recently come to the forefront as functional non–protein-coding RNAs that are involved in a variety of cellular processes ranging from maintaining the structural integrity of chromosomes to gene expression regulation in a spatiotemporal manner. Many recent studies have reported the involvement of lncRNAs in HCC which has led to a better understanding of the underlying molecular mechanisms operating in HCC. Long noncoding RNAs have been shown to regulate development and progression of HCC, and thus, lncRNAs have both diagnostic and therapeutic potentials. In this review, we present an overview of the lncRNAs involved in different stages of HCC and their potential in clinical applications which have been studied so far.

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Abstract 171: Deciphering the role of long noncoding RNAs in the progression of hepatocellular carcinoma using next-generation sequencing

10.1158/1538-7445.am2015-171 ◽

2015 ◽

Author(s):

Davide Degli Esposti ◽

Catherine Voegele ◽

Vincent Cahais ◽

Nathalie Forey ◽

Florence Le Calvez Kelm ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Next Generation Sequencing ◽

Noncoding Rnas ◽

Long Noncoding Rnas ◽

Next Generation ◽

Generation Sequencing

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RNA sequencing analysis of altered expression of long noncoding RNAs associated with Schistosoma japonicum infection in the murine liver and spleen

Parasites & Vectors ◽

10.1186/s13071-020-04457-9 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Tianqi Xia ◽

Bikash Ranjan Giri ◽

Jingyi Liu ◽

Pengfei Du ◽

Xue Li ◽

...

Keyword(s):

Rna Sequencing ◽

Schistosoma Japonicum ◽

Target Genes ◽

Noncoding Rnas ◽

Long Noncoding Rnas ◽

Differentially Expressed ◽

Protein Coding ◽

Altered Expression ◽

Protein Coding Genes

Abstract Background Schistosomiasis is a chronic, debilitating infectious disease caused by members of the genus Schistosoma. Previous findings have suggested a relationship between infection with Schistosoma spp. and alterations in the liver and spleen of infected animals. Recent reports have shown the regulatory role of noncoding RNAs, such as long noncoding RNAs (lncRNAs), in different biological processes. However, little is known about the role of lncRNAs in the mouse liver and spleen during Schistosoma japonicum infection. Methods In this study, we identified and investigated lncRNAs using standard RNA sequencing (RNA-Seq). The biological functions of the altered expression of lncRNAs and their target genes were predicted using bioinformatics. Ten dysregulated lncRNAs were selected randomly and validated in reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) experiments. Results Our study identified 29,845 and 33,788 lncRNAs from the liver and spleen, respectively, of which 212 were novel lncRNAs. We observed that 759 and 789 of the lncRNAs were differentially expressed in the respective organs. The RT-qPCR results correlated well with the sequencing data. In the liver, 657 differentially expressed lncRNAs were predicted to target 2548 protein-coding genes, whereas in the spleen 660 differentially expressed lncRNAs were predicted to target 2673 protein-coding genes. Moreover, functional annotation showed that the target genes of the differentially expressed lncRNAs were associated with cellular processes, metabolic processes, and binding, and were significantly enriched in metabolic pathways, the cell cycle, ubiquitin-mediated proteolysis, and pathways in cancer. Conclusions Our study showed that numerous lncRNAs were differentially expressed in S. japonicum-infected liver and spleen compared to control liver and spleen; this suggested that lncRNAs may be involved in pathogenesis in the liver and spleen during S. japonicum infection.

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