scholarly journals Non-unions treated with bone morphogenic protein 7: introducing the quantitative measurement of human serum cytokine levels as promising tool in evaluation of adjunct non-union therapy

2016 ◽  
Vol 13 (1) ◽  
Author(s):  
Arash Moghaddam ◽  
Lisa Breier ◽  
Patrick Haubruck ◽  
Daniel Bender ◽  
Bahram Biglari ◽  
...  
Keyword(s):  
Human Serum ◽  
Quantitative Measurement ◽  
Bone Morphogenic Protein ◽  
Serum Cytokine ◽  
Non Union ◽  
Promising Tool ◽  
Cytokine Levels ◽  
Bone Morphogenic Protein 7

scholarly journals Defective Proliferation and Osteogenic Potential with Altered Immunoregulatory phenotype of Native Bone marrow-Multipotential Stromal Cells in Atrophic Fracture Non-Union

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jehan J. El-Jawhari ◽  
George Kleftouris ◽  
Yasser El-Sherbiny ◽  
Hany Saleeb ◽  
Robert M. West ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Fracture Healing ◽  
Stromal Cells ◽  
Long Bone ◽  
Osteogenic Potential ◽  
Serum Cytokine ◽  
Non Union ◽  
Native Bone ◽  
Cytokine Levels ◽  
Complicated Fracture

AbstractBone marrow-Multipotential stromal cells (BM-MSCs) are increasingly used to treat complicated fracture healing e.g., non-union. Though, the quality of these autologous cells is not well characterized. We aimed to evaluate bone healing-related capacities of non-union BM-MSCs. Iliac crest-BM was aspirated from long-bone fracture patients with normal healing (U) or non-united (NU). Uncultured (native) CD271highCD45low cells or passage-zero cultured BM-MSCs were analyzed for gene expression levels, and functional assays were conducted using culture-expanded BM-MSCs. Blood samples were analyzed for serum cytokine levels. Uncultured NU-CD271highCD45low cells significantly expressed fewer transcripts of growth factor receptors, EGFR, FGFR1, and FGRF2 than U cells. Significant fewer transcripts of alkaline phosphatase (ALPL), osteocalcin (BGLAP), osteonectin (SPARC) and osteopontin (SPP1) were detected in NU-CD271highCD45low cells. Additionally, immunoregulation-related markers were differentially expressed between NU- and U-CD271highCD45low cells. Interestingly, passage-zero NU BM-MSCs showed low expression of immunosuppressive mediators. However, culture-expanded NU and U BM-MSCs exhibited comparable proliferation, osteogenesis, and immunosuppression. Serum cytokine levels were found similar for NU and U groups. Collectively, native NU-BM-MSCs seemed to have low proliferative and osteogenic capacities; therefore, enhancing their quality should be considered for regenerative therapies. Further research on distorted immunoregulatory molecules expression in BM-MSCs could potentially benefit the prediction of complicated fracture healing.

An Examination of Generalized Pustular Psoriasis in Pregnancy Based on the Serum Cytokine Levels.

1999 ◽  
Vol 61 (2) ◽  
pp. 144-147
Author(s):  
Miyako KUDO ◽  
Yoshihiro UMEBAYASHI ◽  
Tomoe KOTSUJI ◽  
Fujio OTSUKA
Keyword(s):  
Pustular Psoriasis ◽  
Serum Cytokine ◽  
Cytokine Levels ◽  
Generalized Pustular Psoriasis ◽  
In Pregnancy

scholarly journals Reactive arthritis and undifferentiated peripheral spondyloarthritis share human leucocyte antigen B27 subtypes and serum and synovial fluid cytokine profiles

Rheumatology ◽  
2020 ◽  
Author(s):  
Jyoti Ranjan Parida ◽  
Sandeep Kumar ◽  
Sakir Ahmed ◽  
Smriti Chaurasia ◽  
Ratnadeep Mukherjee ◽  
...  
Keyword(s):  
Statistical Difference ◽  
Human Leucocyte Antigen ◽  
Healthy Controls ◽  
Hla B27 ◽  
Serum Cytokine ◽  
Cytokine Profiles ◽  
Cytokine Levels ◽  
Peripheral Spondyloarthritis ◽  
Multiplex Cytokine ◽  
Age And Sex

Abstract Objectives Peripheral SpA (pSpA) is comprised of ReA, PsA, enteritis-associated arthritis and undifferentiated pSpA (upSpA). ReA and upSpA share T cell oligotypes and metabolomics in serum and SF. We investigated HLA-B27 subtypes and cytokines in serum and SF that were compared between ReA and upSpA. Methods ReA and upSpA were compared in two cohorts. In cohort I (44 ReA and 56 upSpA), HLA-B27 subtyping was carried out. In cohort II (17 ReA and 21 upSpA), serum and SF cytokines were compared using a multiplex cytokine bead assay (27 cytokines). A total of 28 healthy controls with similar age and sex to cohort II were included for comparison of serum cytokine levels. Results In cohort I, HLA-B27 was positive in 81.8% (36/44) of ReA and 85.71% (48/56) of upSpA patients. HLA-B27 typing was successful in 70 patients (30 ReA and 40 uSpA). HLA-B*2705 was the most common, followed by HLA-B*2704 and HLA-B*2707. Frequencies were the same between ReA and upSpA. In cohort II, 14 cytokines were detectable in the serum of patients. The levels of eight cytokines were higher than in the controls. The cytokine levels of ReA and upSpA were similar. Sixteen cytokines were detectable in the SF of patients. There was no statistical difference in the levels between ReA and upSpA. The cytokine profiles in sera and SF were also similar among HLA-B27-positive and negative patients. Conclusion ReA and upSpA have similar HLA-B27 subtype associations and similar cytokine profiles. They should be considered as a single entity during studies as well as clinical management.

scholarly journals Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7

2014 ◽  
Vol 15 (1) ◽  
pp. 38 ◽  
Author(s):  
Ann-Marie Heinonen ◽  
Mahbubur Rahman ◽  
Godwin Dogbevia ◽  
Hannah Jakobi ◽  
Stefan Wölfl ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Bone Morphogenic Protein ◽  
Bone Morphogenic Protein 7

Bone morphogenic protein-7 inhibits progression of chronic renal fibrosis associated with two genetic mouse models

2003 ◽  
Vol 285 (6) ◽  
pp. F1060-F1067 ◽  
Author(s):  
Michael Zeisberg ◽  
Cindy Bottiglio ◽  
Navin Kumar ◽  
Yohei Maeshima ◽  
Frank Strutz ◽  
...  
Keyword(s):  
Mouse Models ◽  
Renal Injury ◽  
Renal Fibrosis ◽  
Type I Collagen ◽  
Chronic Renal Disease ◽  
Bone Morphogenic Protein ◽  
Matrix Metalloproteinase 2 ◽  
Type I ◽  
Protective Function ◽  
Bone Morphogenic Protein 7

Tubulointerstitial fibrosis is a hallmark feature of chronic renal injury. Specific therapies to control the progression of renal fibrosis toward end-stage renal failure are limited. Previous studies have demonstrated that expression of endogenous bone morphogenic protein-7 (BMP-7) is reduced in the kidneys of several inducible mouse models of acute and chronic renal disease and that administration of exogenous recombinant human BMP-7 (rhBMP-7) has a beneficial effect on kidney function. Here we report that treatment with rhBMP-7 leads to improved renal function, histology, and survival in mice deficient in the α3-chain of type IV collagen and MRL/MpJ lpr/lpr lupus mice, two genetic models for chronic renal injury and fibrosis. Such therapeutic benefit is also associated with a significant decrease in the expression of profibrotic molecules, such as type I collagen and fibronectin, in renal fibroblasts. Additionally, rhBMP-7 induces expression of active matrix metalloproteinase-2, which is potentially important for removal of fibrotic matrix. Collectively, these studies provide further evidence for rhBMP-7 as an important bone-associated protein with protective function against renal pathology.

Modulation of serum cytokine levels by a novel superoxide dismutase mimetic, M40401, in an Escherichia coli model of septic shock: Correlation with preserved circulating catecholamines*

2003 ◽  
Vol 31 (1) ◽  
pp. 237-245 ◽  
Author(s):  
Heather Macarthur ◽  
Daniel M. Couri ◽  
Gerald H. Wilken ◽  
Thomas C. Westfall ◽  
Andrew J. Lechner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Septic Shock ◽  
Superoxide Dismutase ◽  
Serum Cytokine ◽  
Cytokine Levels
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