scholarly journals Self-assembled nanoparticles containing photosensitizer and polycationic brush for synergistic photothermal and photodynamic therapy against periodontitis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Enyu Shi ◽  
Liya Bai ◽  
Lujia Mao ◽  
Hanping Wang ◽  
Xiaoying Yang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Chronic Inflammatory Disease ◽  
Bacterial Cells ◽  
Sprague Dawley ◽  
In Vivo Evaluation ◽  
Self Assembled ◽  
Bacterial Cell Membrane ◽  
Pass Through

Abstract Background Periodontitis is a chronic inflammatory disease in oral cavity owing to bacterial infection. Photothermal therapy (PTT) and photodynamic therapy (PDT) have many advantages for antibacterial treatment. As an excellent photosensitizer, indocyanine green (ICG) shows prominent photothermal and photodynamic performances. However, it is difficult to pass through the negatively charged bacterial cell membrane, thus limiting its antibacterial application for periodontitis treatment. Results In this work, self-assembled nanoparticles containing ICG and polycationic brush were prepared for synergistic PTT and PDT against periodontitis. First, a star-shaped polycationic brush poly(2-(dimethylamino)ethyl methacrylate) (sPDMA) was synthesized via atom transfer radical polymerization (ATRP) of DMA monomer from bromo-substituted β-cyclodextrin initiator (CD-Br). Next, ICG was assembled with sPDMA to prepare ICG-loaded sPDMA (sPDMA@ICG) nanoparticles (NPs) and the physicochemical properties of these NPs were characterized systematically. In vitro antibacterial effects of sPDMA@ICG NPs were investigated in porphyromonas gingivalis (Pg), one of the recognized periodontitis pathogens. A ligature-induced periodontitis model was established in Sprague–Dawley rats for in vivo evaluation of anti-periodontitis effects of sPDMA@ICG NPs. Benefiting from the unique brush-shaped architecture of sPDMA polycation, sPDMA@ICG NPs significantly promoted the adsorption and penetration of ICG into the bacterial cells and showed excellent PTT and PDT performances. Both in vitro and in vivo, sPDMA@ICG NPs exerted antibacterial and anti-periodontitis actions via synergistic PTT and PDT. Conclusions A self-assembled nanosystem containing ICG and polycationic brush has shown promising clinical application for synergistic PTT and PDT against periodontitis. Graphical Abstract

scholarly journals Self-assembled Nanoparticles of Polycationic b Rush and p Hotosensitizer for Synergistic Photothermal and Photodynamic Therapies Against Periodontitis

2021 ◽  
Author(s):  
Enyu Shi ◽  
Liya Bai ◽  
Lujia Mao ◽  
Hanping Wang ◽  
Xiaoying Yang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Photothermal Therapy ◽  
Chronic Inflammatory Disease ◽  
Bacterial Cells ◽  
Antibacterial Effects ◽  
Self Assembled ◽  
Bacterial Cell Membrane ◽  
Pass Through

Abstract Background: Periodontitis is a chronic inflammatory disease in oral cavity owing to bacterial infection. Photothermal therapy (PTT) and photodynamic therapy (PDT) have many advantages for antibacterial treatment. As an excellent photosensitizer, indocyanine green (ICG) shows prominent photothermal and photodynamic performances. However, it is difficult to pass through the negatively charged bacterial cell membrane, thus limits its antibacterial efficacy for periodontitis treatment.Results: In this work, we developed a nanosystem from self-assembly of ICG and polycationic brush for synergistic PTT and PDT against periodontitis. A star-shaped polycationic brush, poly(2-(dimethylamino)ethyl methacrylate) (sPDMA), was synthesized via atom transfer radical polymerization (ATRP) of DMA monomer from bromo-substituted β-cyclodextrin initiator (CD-Br). ICG was then self-assembled with sPDMA to form ICG-loaded sPDMA (sPDMA@ICG) nanoparticles (NPs), and the physicochemical properties of these NPs were characterized in detail. In vitro antibacterial effects of sPDMA@ICG NPs were evaluated in porphyromonas gingivalis (Pg), one of the recognized periodontitis pathogens, and in vivo anti-periodontitis effects of NPs were investigated in a rat periodontitis model. Benefiting from the unique brush-shaped architecture of sPDMA polycation, sPDMA@ICG NPs efficiently delivered ICG into the bacterial cells through promoting their adsorption and penetration abilities, and also exhibited effective antibacterial and anti-periodontitis actions via synergistic PTT and PDT both in vitro and in vivo.Conclusions: This work developed a promising nano-photosensitizer for synergistic PTT and PDT for antibacterial and periodontitis treatments in clinic.

Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy

2020 ◽  
Vol 63 (17) ◽  
pp. 9950-9964 ◽  
Author(s):  
Zhiliang Yu ◽  
Junliang Zhou ◽  
Xin Ji ◽  
Guangyu Lin ◽  
Shuang Xu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
In Vivo Evaluation

scholarly journals Enhanced Oral Bioavailability of Celecoxib Nanocrystalline Solid Dispersion based on Wet Media Milling Technique: Formulation, Optimization and In Vitro/In Vivo Evaluation

Pharmaceutics ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 328 ◽  
Author(s):  
Zhuang Ding ◽  
Lili Wang ◽  
Yangyang Xing ◽  
Yanna Zhao ◽  
Zhengping Wang ◽  
...  
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Solid Dispersion ◽  
Oral Bioavailability ◽  
Stability Study ◽  
Sprague Dawley ◽  
Scanning Calorimetry ◽  
In Vivo Evaluation

Celecoxib (CLX), a selective COX-2 inhibitor, is a biopharmaceutics classification system (BCS) class II drug with its bioavailability being limited by thepoor aqueoussolubility. The purpose of this study was to develop and optimize CLX nanocrystalline(CLX-NC) solid dispersion prepared by the wet medium millingtechnique combined with lyophilizationto enhance oral bioavailability. In formulation screening, the resulting CLX-NC usingpolyvinylpyrrolidone (PVP) VA64 and sodiumdodecyl sulfate (SDS) as combined stabilizers showed the minimum particle size and a satisfactory stability. The formulation and preparation processwere further optimized by central composite experimentaldesign with PVP VA64 concentration (X1), SDS concentration (X2) and milling times (X3) as independent factors and particle size (Y1), polydispersity index (PDI, Y2) and zeta potential (Y3) as response variables. The optimal condition was determined as a combination of 0.75% PVP VA64, 0.11% SDS with milling for 90 min.The particle size, PDI and zeta potential of optimized CLX-NC were found to be 152.4 ± 1.4 nm, 0.191 ± 0.012 and −34.4 ± 0.6 mV, respectively. The optimized formulation showed homogeneous rod-like morphology as observed by scanning electron microscopy and was in a crystalline state as determined by differential scanning calorimetry and powder X-ray diffraction. In a storage stability study, optimized CLX-NC exhibited an excellent physical stability during six months’ storage at both the refrigeration and room conditions. In vivo pharmacokinetic research in Sprague-Dawley ratsdisplayed that Cmax and AUC0–∞ of CLX-NC were increased by 2.9 and 3.1 fold, compared with physical mixture. In this study, the screening and optimizing strategy of CLX-NC formulation represents a commercially viable approach forenhancing the oral bioavailability of CLX.

scholarly journals Biofilm Inactivation using Photodynamic Therapy in Dentistry: a review of literature

2020 ◽  
Vol 11 (Vol.11, no.3) ◽  
pp. 279-287
Author(s):  
Corina-Elena TIȘLER ◽  
Mîndra-Eugenia BADEA ◽  
Smaranda BUDURU ◽  
Andreea KUI ◽  
Mihaela FLORIA ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Chemical Substance ◽  
Review Of Literature ◽  
In Vivo Evaluation ◽  
Specific Effects ◽  
Pubmed Database ◽  
Microorganism Inactivation ◽  
Additional Procedure

Introduction: Photodynamic therapy (PDT) is a therapy involving light and a photosensitising chemical substance, used in conjunction with molecular oxygen in order to elicit cell death (photo-toxicity) and thus ability to kill microbial cells, including bacteria, fungi and viruses. Photodynamic therapy is an alternative method of biofilm disruption and it is considered a new way of microorganism inactivation. It is also an additional procedure to reduce the infection rate in patients, caused by the increasing antimicrobials resistance of bacteria. The aim of this literature review was to evaluate the specific effects and the antibacterial effectiveness of photodynamic therapy using different types of photosensitizers (Erythrosine, Rose Bengal, Toluidine blue, Methylene blue, Ozone, Riboflavin, Curcumin, Chlorhexidine, SAPYR) and a visible light of a specific wavelength for each photosensitizer and to reveal the applications of PDT in periodontics, endodontics, prosthodontics and dental caries. Methods: A research of literature was performed in an attempt to find all the articles published on this topic in the last 10 years. The articles was searched by using a certain combination of different keywords (photodynamic therapy ) and (diode laser ) and (teeth) in PubMed database. Results: A total number of 83 articles were found. After applying inclusion and exclusion criteria, 35 articles were taken into consideration for our study and among them 4 were a manuscript, 3 was a review of literature, 1 was an in vivo evaluation and 27 were in vitro studies. Conclusion: Considering that none of the disinfection methods can completely remove the biofilm, PDT is a therapeutic tool complementary to conventional disinfection, with great applicability in dentistry. PDT showed significantly efficacy in reduction of biofilms. Exposure to light in the presence of a photosensitizing chemical substance helps in the reduction of microbes and the protocols could be recommended for clinical usage, but only together with ‘classic ‘ disinfection.

In vitro and in vivo evaluation of Radachlorin® sensitizer for photodynamic therapy

2009 ◽  
Vol 8 (3) ◽  
pp. 405 ◽  
Author(s):  
Samuel Douillard ◽  
David Olivier ◽  
Thierry Patrice
Keyword(s):  
Photodynamic Therapy ◽  
In Vivo Evaluation

In vitro and in vivo evaluation of improved EGFR targeting peptide-conjugated phthalocyanine photosensitizers for tumor photodynamic therapy

2018 ◽  
Vol 29 (7) ◽  
pp. 1171-1178 ◽  
Author(s):  
Qingle Chen ◽  
Yanhong Ma ◽  
Jisi Zhao ◽  
Mei Zhao ◽  
Wenjing Li ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vivo Evaluation ◽  
Targeting Peptide

Synthesis, and in vitro and in vivo evaluation of a diphenylchlorin sensitizer for photodynamic therapy

2003 ◽  
Vol 69 (3) ◽  
pp. 179-192 ◽  
Author(s):  
L Bourré ◽  
G Simonneaux ◽  
Y Ferrand ◽  
S Thibaut ◽  
Y Lajat ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vivo Evaluation

Preclinical evaluation of novel CDK4/6 inhibitor GLR2007 in glioblastoma models.

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. e14023-e14023
Author(s):  
Lei Yin ◽  
Zhenglin Yao ◽  
Yue Wang ◽  
Julius Huang ◽  
Michelle Mazuranic ◽  
...  
Keyword(s):  
Cell Lines ◽  
Vehicle Control ◽  
Whole Body ◽  
In Vitro Assays ◽  
G1 Arrest ◽  
Sprague Dawley ◽  
In Vivo Evaluation ◽  
Xenograft Models

e14023 Background: Glioblastoma multiforme (GBM) is characterized by a high frequency of cyclin-dependent kinase (CDK)4 and CDK6 pathway dysregulation. CDK4/6 inhibitors palbociclib and abemaciclib are approved for the treatment of breast cancer, but poor blood–brain barrier (BBB) penetration limits their efficacy in GBM. GLR2007 is a novel CDK4/6 inhibitor with potential for improved penetration across the BBB. Here, we report on the activity of GLR2007 in GBM cell lines and its anti-tumor efficacy in mouse xenograft models. Methods: Three in vitro assays were used to assess the activity of GLR2007. Inhibition of CDK4/6 enzymatic activity by GLR2007 or palbociclib was calculated, and cell cycle stages were analyzed in U87-MG cells treated with vehicle control or GLR2007 for 24 h. Cell viability was evaluated in U87-MG and U118-MG cell lines after culture for 72 h with GLR2007 or abemaciclib. In vivo evaluation of the anti-tumor efficacy of GLR2007 versus vehicle, abemaciclib, and/or palbociclib was performed in BALB/c nude mouse GBM xenograft models. Quantitative whole-body autoradiography was used to determine the distribution of [14C]GLR2007 in the tissues of Sprague Dawley rats. Results: GLR2007 potency toward CDK4 and CDK6 was 33.1 and 3.8 times that of palbociclib, respectively. At concentrations >13.72 nM, GLR2007 caused G1 arrest of U87-MG cells. GLR2007 inhibited proliferation in U87-MG cells (IC50 15.6±2.4 nM) and U118-MG cells (IC50 23.2±5.2 nM). Anti-tumor efficacy of GLR2007 versus vehicle control was observed in two mouse GBM xenograft models (Table). Studies performed in rats demonstrated the distribution of [14C]GLR2007 in whole brain tissue following a single oral dose, with total radioactivity levels in the brain exceeding those in plasma by 2.3–4.5-fold from 2–6 h after dosing. Conclusions: These preclinical studies demonstrate the potential of GLR2007 as a novel CDK4/6 inhibitor for treatment of GBM. GLR2007 showed numerically greater anti-tumor efficacy than approved CDK4/6 inhibitors in xenograft models, and evidence of substantial central nervous system penetration. [Table: see text]

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