scholarly journals Macronutrient, immunoglobulin a and total antioxidant capacity profiles of human milk from 1 to 24 months: a cross-sectional study in Thailand

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Krongporn Ongprasert ◽  
Jetsada Ruangsuriya ◽  
Rungnapa Malasao ◽  
Ratana Sapbamrer ◽  
Pikul Suppansan ◽  
...  
Keyword(s):  
Human Milk ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Energy Content ◽  
Immunoglobulin A ◽  
Milk Composition ◽  
Cross Sectional ◽  
Milk Samples ◽  
Carbohydrate Concentration ◽  
Total Antioxidant

Abstract Background An extended duration of breastfeeding of up to two years is encouraged by many health authorities, but information regarding the composition of milk after one year postpartum is limited. The goal of this study was to determine the association between the duration of lactation and macronutrient contents, immunoglobulin A (IgA) levels and total antioxidant capacity (TAC) in human milk (HM), from 1 to 24 months postpartum. Methods Cross-sectional milk samples were collected between January and April 2019 from mothers with healthy full-term children who had been lactating for 1 to 24 months. The HM was biochemically analyzed for protein and carbohydrate contents by colorimetric assays. The fat content was determined by capillary centrifugation, and the energy content was calculated from the results of centrifugation assays. IgA levels and TAC were determined by ELISA and a Trolox equivalent antioxidant capacity (TEAC) assay, respectively. Pearson’s correlation coefficient and Spearman’s rank correlation coefficient were used to determine associations between months of lactation and milk composition, and multiple regression analysis was used to assess associations between months of lactation and milk composition adjusted for relevant covariates. Differences were considered significant at p < 0.05. Results One hundred eighty-four milk samples were analyzed. The month of lactation was positively associated with the fat concentration (B = 0.31, SE = 0.09, p = 0.001), energy content (B = 3.11, SE = 0.92, p = 0.001), and IgA (B = 4.17, SE = 1.08, p < 0.001) but negatively associated with the carbohydrate concentration (B = − 0.22, SE = 0.01, p = 0.04). No association was observed between the month of lactation and the protein concentration or TAC after adjustment for maternal age, maternal BMI, birth order, and breastfeeding frequency. Conclusion The duration of lactation was found to be positively associated with the fat, energy, and IgA content in HM for up to two years postpartum, and negatively associated with carbohydrate concentration. More prospective cohort studies are needed to obtain evidence-based knowledge regarding the changes in HM composition throughout the course of lactation.

scholarly journals Macronutrient, immunoglobulin A and total antioxidant capacity profiles of human milk: cross-sectional surveys at ages 6, 12, 18 and 24 months.

2020 ◽  
Author(s):  
krongporn ongprasert ◽  
Jetsada Ruangsuriya ◽  
Rungnapa Malasao ◽  
Ratana Sapbamrer ◽  
Pikul Suppansan ◽  
...  
Keyword(s):  
Human Milk ◽  
Antioxidant Capacity ◽  
Correlation Coefficient ◽  
Total Antioxidant Capacity ◽  
Energy Content ◽  
Immunoglobulin A ◽  
Milk Composition ◽  
Milk Samples ◽  
Carbohydrate Concentration ◽  
Total Antioxidant

Abstract Background: A longer duration of breastfeeding of up to two years is encouraged by many health authorities, but information regarding the composition of milk after one year postpartum is limited. The goal of this study was to determine the associations of macronutrient contents, immunoglobulin A (IgA) levels, and the total antioxidant capacity (TAC) in human milk (HM) with the month of lactation from 1 to 24 months postpartum.Methods: Milk samples were collected from mothers with healthy full-term children who had been lactating from 1 to 24 months from January 2019 to April 2019. HM was biochemically analyzed for protein and carbohydrate contents by colorimetric assays. The fat content was determined by capillary centrifugation, and the energy content was calculated from the results of centrifugation assays. IgA levels and the TAC were determined by ELISA and a Trolox equivalent antioxidant capacity (TEAC) assay, respectively. Pearson’s correlation coefficient and Spearman’s rank correlation coefficient were used to determine associations between milk composition and the month of lactation, and multiple regression analysis was used to assess the association between covariates and milk composition. Differences were considered significant at p < 0.05.Results: One hundred eighty-four milk samples were analyzed. The month of lactation was positively associated with the fat concentration (B = 0.31, SE = 0.09, p = 0.001), energy content (B = 3.11, SE = 0.92, p = 0.001), and IgA (B = 4.17, SE = 1.08, p < 0.001) but negatively associated with the carbohydrate concentration (B = -0.22, SE = 0.01, p = 0.04). No association was observed between the month of lactation and the protein concentration and TAC after adjustment for maternal age, maternal BMI, birth order, and breastfeeding frequency.Conclusions: Based on our results, fat, energy, and IgA contents in HM were positively associated with the month of lactation, and a slight but significant negative association was detected for the carbohydrate concentration up to two years postpartum.

scholarly journals Macronutrient, immunoglobulin A and total antioxidant capacity profiles of human milk: cross-sectional surveys at ages 6, 12, 18 and 24 months.

2020 ◽  
Author(s):  
Krongporn Ongprasert ◽  
Jetsada Ruangsuriya ◽  
Rungnapa Malasao ◽  
Ratana Sapbamrer ◽  
Pikul Suppansan ◽  
...  
Keyword(s):  
Human Milk ◽  
Antioxidant Capacity ◽  
Correlation Coefficient ◽  
Total Antioxidant Capacity ◽  
Energy Content ◽  
Immunoglobulin A ◽  
Milk Composition ◽  
Milk Samples ◽  
Carbohydrate Concentration ◽  
Total Antioxidant

Abstract Background: An extended duration of breastfeeding of up to two years is encouraged by many health authorities, but information regarding the composition of milk after one year postpartum is limited. The goal of this study was to determine the associations of macronutrient contents, immunoglobulin A (IgA) levels, and the total antioxidant capacity (TAC) in human milk (HM) with the month of lactation from 1 to 24 months postpartum.Methods: Milk samples were collected from mothers with healthy full-term children who had been lactating from 1 to 24 months from January 2019 to April 2019. The HM was biochemically analyzed for protein and carbohydrate contents by colorimetric assays. The fat content was determined by capillary centrifugation, and the energy content was calculated from the results of centrifugation assays. IgA levels and the TAC were determined by ELISA and a Trolox equivalent antioxidant capacity (TEAC) assay, respectively. Pearson’s correlation coefficient and Spearman’s rank correlation coefficient were used to determine associations between milk composition and the month of lactation, and multiple regression analysis was used to assess the association between the covariates and milk composition. Differences were considered significant at p < 0.05.Results: One hundred eighty-four milk samples were analyzed. The month of lactation was positively associated with the fat concentration (B = 0.31, SE = 0.09, p = 0.001), energy content (B = 3.11, SE = 0.92, p = 0.001), and IgA (B = 4.17, SE = 1.08, p < 0.001) but negatively associated with the carbohydrate concentration (B = -0.22, SE = 0.01, p = 0.04). No association was observed between the month of lactation and the protein concentration and TAC after adjustment for maternal age, maternal BMI, birth order, and breastfeeding frequency.Conclusions: The month of lactation was found to be positively associated with the fat, energy, and IgA in HM for up to two years postpartum and slightly but significantly negatively associated with carbohydrate concentration. More prospective cohort studies are needed to obtain evidence-based knowledge regarding the changes in HM composition throughout the course of lactation.

scholarly journals Macronutrient, immunoglobulin A and total antioxidant capacity profiles of human milk: cross-sectional surveys at ages 6, 12, 18 and 24 months.

2020 ◽  
Author(s):  
krongporn ongprasert ◽  
Jetsada Ruangsuriya ◽  
Rungnapa Malasao ◽  
Ratana Sapbamrer ◽  
Pikul Suppansan ◽  
...  
Keyword(s):  
Human Milk ◽  
Antioxidant Capacity ◽  
Correlation Coefficient ◽  
Total Antioxidant Capacity ◽  
Immunoglobulin A ◽  
Milk Composition ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Carbohydrate Concentration ◽  
Total Antioxidant ◽  
Extended Lactation

Abstract Background: A longer duration of breastfeeding of up to two years is encouraged by many health authorities, but there is limited information regarding the composition of milk after one year postpartum. The goal of this study was to determine the association between human milk macronutrient, immunoglobulin A (IgA), and total antioxidant capacity (TAC) profiles during extended lactation.Methods: One hundred eighty-four breastfeeding mothers with full-term healthy children who had been lactating from 1 to 24 months were recruited from January 2019 to April 2019. Human milk was biochemically analyzed for protein and carbohydrate content by colorimetric assays. The fat content was determined by capillary centrifugation, and the energy content was calculated from the results of centrifugation assays. IgA levels and TAC were determined by ELISA and a Trolox equivalent antioxidant capacity (TEAC) assay, respectively. Pearson’s correlation coefficient and Spearman’s rank correlation coefficient were used to determine associations between milk composition with month of lactation, and multiple regression analysis was used to assess the association between covariate variables and milk composition. Differences were considered significant at p < 0.05.Results: The fat, energy and IgA contents were positively correlated with the duration of lactation (r = 0.229, p = 0.002; r = 0.229, p =0.002 and r = 0.304, p < 0.001, respectively). No significant correlations between protein, carbohydrate concentrations and TAC with the duration of lactation were observed (r = 0.106, p = 0.15; r = -0.032, p = 0.67; r = -0.056, p = 0.45, respectively). After adjusting for the covariates, the month of lactation was negatively associated with the carbohydrate concentration (p = 0.04), while similar results were observed for other components.Conclusions: We demonstrated that fat, energy, and IgA contents increased during extended lactation lasting up to two years postpartum. A slightly but significant decrease was detected in carbohydrate concentration. No association was observed in protein concentration and TAC with the duration of lactation. Based on these results, lactating mothers should be encouraged and supported to continue breastfeeding for at least two years postpartum.

scholarly journals Macronutrients, immunoglobulin A and total antioxidant capacity of human milk during prolonged lactation

2019 ◽  
Author(s):  
krongporn ongprasert ◽  
Jetsada Ruangsuriya ◽  
Rungnapa Malasao ◽  
Ratana Sapbamrer ◽  
Pikul Suppansan ◽  
...  
Keyword(s):  
Human Milk ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Energy Content ◽  
Immunoglobulin A ◽  
Milk Composition ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Healthy Children ◽  
Limited Information ◽  
Total Antioxidant

Abstract Background: A longer duration of breastfeeding of up to two years is encouraged by many health authorities, but there is limited information regarding the composition of the milk after one year postpartum. The aim of the study was to determine the longitudinal changes in human milk macronutrients, immunoglobulin A (IgA) and total antioxidant capacity (TAC) during prolonged lactation. Methods: One hundred eighty-four breastfeeding mothers with full-term healthy children who had been lactating from 1 to 24 months were recruited from January 2019 to April 2019. Human milk was biochemically analyzed for protein and carbohydrate content by colorimetric assays. The fat content was determined by capillary centrifugation, and the energy content was calculated from the results of centrifugation. IgA levels and TAC were determined by ELISA and a Trolox equivalent antioxidant capacity (TEAC) assay, respectively.Pearson correlation analysis was used to determine the association of milk composition with the month of lactation. Results: The concentrations of protein, fat, energy and IgA were positively correlated with the duration of lactation ( r = 0.15, p < 0.05; r = 0.23, p < 0.05; r = 0.23, p < 0.05 and r = 0.29, p < 0.05, respectively). No significant correlations of the carbohydrate concentrations or TAC with the duration of lactation was observed ( r = 0.10, p > 0.05 and r = -0.07, P > 0.05, respectively). Conclusions: We demonstrated that protein, fat, IgA and energy contents increasedduring prolonged lactation lasting up to twoyears postpartum, while carbohydrateconcentrationand TACwerenot related to lactation duration. Based on the results, lactating mothers should be encouraged to continue breastfeeding for at least two years postpartum.

scholarly journals Macronutrient, immunoglobulin A and total antioxidant capacity profiles of human milk from 1 to 24 months postpartum

2020 ◽  
Author(s):  
krongporn ongprasert ◽  
Jetsada Ruangsuriya ◽  
Rungnapa Malasao ◽  
Ratana Sapbamrer ◽  
Pikul Suppansan ◽  
...  
Keyword(s):  
Human Milk ◽  
Antioxidant Capacity ◽  
Correlation Coefficient ◽  
Total Antioxidant Capacity ◽  
Energy Content ◽  
Immunoglobulin A ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Healthy Children ◽  
Total Antioxidant ◽  
Extended Lactation

Abstract Background: A longer duration of breastfeeding of up to two years is encouraged by many health authorities, but there is limited information regarding the composition of milk after one year postpartum. The goal of this study was to determine the changes in human milk macronutrient, immunoglobulin A (IgA), and total antioxidant capacity (TAC) profiles during extended lactation.Methods: One hundred eighty-four breastfeeding mothers with full-term healthy children who had been lactating from 1 to 24 months were recruited from January 2019 to April 2019. Human milk was biochemically analyzed for protein and carbohydrate content by colorimetric assays. The fat content was determined by capillary centrifugation, and the energy content was calculated from the results of centrifugation assays. IgA levels and TAC were determined by ELISA and a Trolox equivalent antioxidant capacity (TEAC) assay, respectively. Pearson’s correlation coefficient and Spearman’s rank correlation coefficient were used to determine the association of milk composition with month of lactation.Results: The fat, energy and IgA contents were positively correlated with the duration of lactation (r = 0.229, p = 0.002 ; r = 0.229, p =0.002 and r = 0.304, p < 0.001, respectively). No significant correlations between protein, carbohydrate concentrations and TAC with the duration of lactation were observed (r = 0.106, p = 0.15; r = -0.032, p = 0.67; r = -0.056, p = 0.45, respectively).Conclusions: We demonstrated that fat, energy and IgA contents increased during extended lactation lasting up to two years postpartum, while protein and carbohydrate concentrations and TAC were not related to lactation duration. Based on these results, lactating mothers should be encouraged to continue breastfeeding for at least two years postpartum.

scholarly journals Macronutrients, immunoglobulin A and total antioxidant capacity of human milk during prolonged lactation

2020 ◽  
Author(s):  
krongporn ongprasert ◽  
Jetsada Ruangsuriya ◽  
Rungnapa Malasao ◽  
Ratana Sapbamrer ◽  
Pikul Suppansan ◽  
...  
Keyword(s):  
Human Milk ◽  
Antioxidant Capacity ◽  
Correlation Coefficient ◽  
Total Antioxidant Capacity ◽  
Energy Content ◽  
Pearson Correlation ◽  
Immunoglobulin A ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Healthy Children ◽  
Total Antioxidant

Abstract Background: A longer duration of breastfeeding of up to two years is encouraged by many health authorities, but there is limited information regarding the composition of the milk after one year postpartum. The aim of the study was to determine the longitudinal changes in human milk macronutrients, immunoglobulin A (IgA) and total antioxidant capacity (TAC) during prolonged lactation.Methods: One hundred eighty-four breastfeeding mothers with full-term healthy children who had been lactating from 1 to 24 months were recruited from January 2019 to April 2019. Human milk was biochemically analyzed for protein and carbohydrate content by colorimetric assays. The fat content was determined by capillary centrifugation, and the energy content was calculated from the results of centrifugation. IgA levels and TAC were determined by ELISA and a Trolox equivalent antioxidant capacity (TEAC) assay, respectively. Pearson Correlation Coefficient and Spearman’s Rank Correlation Coefficient were used to determine the association of milk composition with month of lactation.Results: The concentrations of fat, energy and IgA were positively correlated with the duration of lactation (r = 0.229, p < 0.01; r = 0.229, p < 0.01 and r = 0.304, p < 0.01, respectively). No significant correlations of protein, carbohydrate concentrations and TAC with the duration of lactation was observed (r = 0.106, p = 0.15; r = -0.032, p = 0.67; r = -0.056, p = 0.45, respectively).Conclusions: We demonstrated that fat, energy and IgA contents increased during prolonged lactation lasting up to two years postpartum, while protein and carbohydrate concentration and TAC were not related to lactation duration. Based on the results, lactating mothers should be encouraged to continue breastfeeding for at least two years postpartum.

scholarly journals The effect of lyophilization on selected biologically active components (vitamin C, catalase, lysozyme), total antioxidant capacity and lipid oxidation in human milk

2017 ◽  
Vol 112 (3) ◽  
pp. 121-128
Author(s):  
Dorota Martysiak-Żurowska ◽  
Małgorzata Puta ◽  
Anna Rodzik ◽  
Edyta Malinowska-Pańczyk
Keyword(s):  
Vitamin C ◽  
Human Milk ◽  
Lipid Oxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Freeze Drying ◽  
Biologically Active ◽  
Initial Water Content ◽  
Initial Water ◽  
Total Antioxidant

Human milk is rich in polyunsaturated fatty acids, as well as lysozyme, vitamin C and other bioactive compounds. The effect of lyophilization on the content of antioxidants (vitamin C and catalase CAT), bactericidal compounds (lysozyme), total antioxidant capacity (TAC) and lipid peroxidation in human milk was investigated in this study. Samples of mature human milk were collected from five healthy women who gave birth on the scheduled date and without complications. Freeze drying resulted in the removal of 88.2 % of the initial water content from milk. The human milk lyophilizate was readily soluble in water. Lyophilization had no effect on the content of primary (lipid peroxides LP) and secondary (thiobarbituric acid reactive substances TBARS) products of lipid oxidation. Freeze-drying led to a significant decrease in the vitamin C content and TAC values of milk (by 31 % and 16.5 %, respectively). Catalase and lysozyme were resistant to freeze-drying. Lyophilization induced a decrease in lysozyme content (9 %) and catalase activity (11 %) but these changes were not statistically significant. Low-temperature dehydration and rehydration of human milk lyophilizates promote satisfactory retention of biologically active ingredients and prevent the oxidation of human milk lipids. The results of this study indicate that lyophilization can be considered as an effective method for prolonging the shelf life of human milk.

P–112 Association between dietary total antioxidant capacity and semen quality parameters in male partners of couples attempting fertility

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
G Eslamian ◽  
S Rohani ◽  
N Shoaibinobarian
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Pattern ◽  
Total Antioxidant Capacity ◽  
Semen Quality ◽  
Sperm Count ◽  
Quality Parameters ◽  
Cross Sectional ◽  
Abnormal Sperm ◽  
Total Antioxidant ◽  
Dietary Total Antioxidant Capacity

Abstract Study question Is there any association between dietary total antioxidant capacity (TAC) and semen quality parameters in male partners of couples attempting fertility? Summary answer Greater adherence to diets high in TAC was significantly associated with higher total sperm count, sperm concentration, and sperm motility. What is known already Among multiple factors that affect the etiology of poor semen quality and male infertility, dietary factors have an important contribution. Also, chronic oxidative stress negatively effects semen quality. Whether adherence to the diet rich in antioxidants is associated with better semen quality remains largely unexplored.The concentration of single antioxidant cannot show the total antioxidant power of the diet, therefore the concept of dietary total antioxidant capacity (TAC) was invented.Dietary scores are useful approach to evaluate the degree of adherence to specific dietary pattern and its benefits in regard to health Study design, size, duration This was a cross-sectional study of 350 men from couples attending a fertility center in Tehran, Iran, recruited between June 2015 and September 2019. Men aged 25–50 years with complete dietary data were analyzed. Participants/materials, setting, methods Diet was assessed via a reproducible and valid 168-item semi-quantitative food frequency questionnaire to determine the entire antioxidants of the usual diet in order to calculate dietary TAC. Semen quality was assessed according to World Health Organization 2010 guidelines. The association between dietary TAC and semen parameters were assessed adjusting for potential confounders through multiple logistic regression analysis. Main results and the role of chance The average age of study participants was 34.9 ± 7.6 years and their BMI was 28.5 ± 4.3 kg/m2. Participants in the lower tertile of the dietary TAC were lower educated (p &lt; 0.01), more physically active (p &lt; 0.05), and predominantly had abnormal sperm progressive motility (p &lt; 0.01). In the multivariable adjusted models, men in the lowest tertile of the dietary TAC Score had 2.9 times higher likelihood of having abnormal sperm motility, total sperm count and concentration, compared to men in the highest tertile of the TAC score (p &lt; 0.05). Limitations, reasons for caution The main limitation of our study was its cross-sectional design, limiting our ability to derive causal association. Wider implications of the findings: Our study suggests that dietary pattern comprising mainly of antioxidant nutrients may help improve semen quality. Our results are consistent with previous studies showing that plant-based diet contains higher levels of antioxidants are associated with better measures of semen quality. Trial registration number Not applicable

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