scholarly journals Prevalence of heterophilic antibodies in serum samples from horses in an equine hospital, and elimination of interference using chicken IgY

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Bo Dong ◽  
Daniel Bergman ◽  
Bodil Ström Holst
Keyword(s):  
Veterinary Medicine ◽  
False Positive ◽  
Horse Serum ◽  
Test Results ◽  
Serum Samples ◽  
Heterophilic Antibodies ◽  
Sandwich Type

Abstract Background Heterophilic antibodies in serum and plasma can interfere with mammalian antibodies in immunoassays and result in false test results, usually false positive. Although studies screening for heterophilic antibodies as well as elimination studies have been conducted in dogs and cats, knowledge of the presence of heterophilic antibodies in other species in veterinary medicine is limited. In this study, a 2-site sandwich-type interference assay that detects anti-mouse antibodies was used to detect heterophilic antibodies in a population of horses treated in an animal hospital. Results A total of 194 serum samples from 127 individual horses were analyzed. There were 11/127 (8.7%) interference-positive horses, and these were analyzed in an assay exchanging the capture mouse IgG with chicken IgY. The positive samples were negative in the chicken IgY assay, indicating elimination of a possible interference, with the chicken-based assay. Four interference-positive samples were from geldings, and anti-Müllerian hormone (AMH) was analyzed from these samples. AMH concentrations were negative in these samples as expected in geldings, indicating that the heterophilic antibodies did not cause interference in the AMH assay. Conclusion The present study shows that there are heterophilic antibodies in horse serum samples like in samples from humans, dogs, and cats. The use of chicken-based reagents, such as chicken IgY, which do not cross-react with mammalian IgG, eliminates the effects of interfering antibodies in the samples. Equine heterophilic antibodies do not necessarily cause interference in commercial immunoassays.

scholarly journals Žmogaus chorioninio gonadotropino reikšmė klinikinėje praktikoje

2013 ◽  
Vol 12 (4) ◽  
pp. 238-242
Author(s):  
Oldas Tamašauskas ◽  
Ieva Šiaudinytė
Keyword(s):  
Ectopic Pregnancy ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
False Positive ◽  
Clinical Status ◽  
Test Results ◽  
Laboratory Equipment ◽  
Heterophilic Antibodies ◽  
Diagnostic Approaches ◽  
Positive Results

Žmogaus chorioninio gonadotropino (hCG) koncentracijos nustatymas dažniausiai naudojamas ankstyvo nėštumo diagnostikai, trukmei nustatyti bei nėštumui stebėti. Be to, HCG yra vertingas diagnozuojant savaiminį persileidimą, nesivystantį arektopinį nėštumą. Šis hormonas leidžia prenataliai įvertinti vaisiaus apsigimimų riziką. Taip pat hCG labai svarbus trofoblastinių ligų ir tam tikrų lokalizacijų vėžio diagnostikai, gydymo efektyvumo vertinimui. Retai, tačiau galimi ir klaidingai teigiamityrimų rezultatai dėl analitinių klaidų, tyrimams naudojamų įrenginių, prietaisų gedimų ar tam tikrų kraujyje esančių medžiagų (pvz.: heterofiliniai antikūnai, autoantikūnai, reumatoidinis faktorius, taip pat bilirubinas, hemoglobinas ar lipidai galilemti klaidingus, netikslius tyrimo rezultatus), todėl svarbu įvertinti ne vieno tyrimo rezultatus, o bendrą klinikinį vaizdą, visą surinktą informaciją ir apsvarstyti visas klinikinės diagnozės galimybes.Reikšminiai žodžiai: žmogaus chorioninis gonadotropinas, hCG, ektopinis nėštumas, klaidingai teigiamas rezultatas, Immulite2000, nėštumo diagnostika.The significance of human chorionic gonadotropin in clinical practice pregnancy, assess its duration and observe its course. In addition, hCG is a valuable tool in the diagnostics of miscarriage, nondeveloping foetus or ectopic pregnancy. The measurements of this hormone allow to suspect the possible risk of foetalabnormalities. hCG is also used to diagnose and follow the course of particular types of cancer and diseases of throphoblastic origin. Rarely, though, it is possible that false positive results ensue due to failures of analysis, malfunction of laboratory equipment or particular substances circulating in the blood, i.e. heterophilic antibodies, autoantibodies, the rheumatoid factor, bilirubin, haemoglobin or lipids could possibly lead to false test results. Taking into consideration all the previously mentionedissues, it is always important to assess the whole clinical status of the patient, to gather all the possible information and to consider various diagnostic approaches and not to rely too heavily on one laboratory test.Key words: human chorionic gonadotropin, ectopic pregnancy, hCG, false-positive rezult, Immulite2000, pregnancy diagnostics

scholarly journals Seroprevalensi Penyakit Egg Drop Syndrome pada Itik di Desa Tumbak Bayuh, Kecamatan Mengwi, Badung, Bali

2019 ◽  
Vol 37 (2) ◽  
pp. 248
Author(s):  
Gusti Ayu Yuniati Kencana ◽  
I Made Kardena ◽  
Ni Wayan Apsari Shantika Pratistha
Keyword(s):  
Veterinary Medicine ◽  
Antibody Titer ◽  
Newcastle Disease ◽  
Test Results ◽  
Serum Samples ◽  
Protective Antibody ◽  
Serological Examination ◽  
Positive Serum ◽  
Antibody Titers

Egg Drop Syndrome can cause detrimental impacts on breeders due to reducing production and quality of theaffected eggs.This study aim was to determine the seroprevalece ofantibody against Egg Drop Syndrome (EDS) virus in ducks. Antibody titers examination was done from 75blood samplesof ducks thathave not been vaccinatedagainstEDS virus. The duck samples were collected from Tumbak Bayuh Village, Mengwi, Badung. Serological examination was held at the Virology Laboratory,Faculty of Veterinary Medicine, Udayana University by usingHaemagglutination Inhibition (HI) test. HI test results showed that 24 samples were positive contained antibody of the EDS,while 51 samples were negative. Range of EDS antibody titer observed was from24 to 27 HI units.  This results indicates that the ducks have protective antibody titer against EDS virus. The positive serum samples were also tested using HI test against Newcastle Disease (ND) virus with negative result. It can be concluded that the ducks in Tumbak Bayuh Village, Mengwi, Badung have 32% of antibody titer against EDS virus whichmight result from being exposed by EDS virus naturally.

scholarly journals Atypical anti-glomerular basement membrane disease with anti-GBM antibody negativity and ANCA positivity: a case report

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Na Guo ◽  
Qinghua Yin ◽  
Song Lei ◽  
Yanjun He ◽  
Ping Fu
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Rare Case ◽  
Test Results ◽  
Serum Samples ◽  
Tissue Samples ◽  
Case Presentation ◽  
Severe Renal Insufficiency ◽  
Laboratory Test Results ◽  
Organ Specific

Abstract Background Anti-glomerular basement membrane (anti-GBM) disease is an organ-specific autoimmune disease that involves the lung and kidneys and leads to rapid glomerulonephritis progression, with or without diffuse alveolar hemorrhage, and even respiratory failure. Classic cases of anti-GBM disease are diagnosed based on the presence of the anti-GBM antibody in serum samples and kidney or lung biopsy tissue samples. However, atypical cases of anti-GBM disease are also seen in clinical practice. Case presentation We herein report the rare case of a patient with atypical anti-GBM disease whose serum was negative for the anti-GBM antibody but positive for the myeloperoxidase (MPO) anti-neutrophil cytoplasmic antibody (p-ANCA) and another atypical ANCA. Laboratory test results showed severe renal insufficiency with a creatinine level of 385 μmol/L. Renal biopsy specimen analysis revealed 100% glomeruli with crescents; immunofluorescence showed immunoglobulin G (IgG) linearly deposited alongside the GBM. Finally, the patient was discharged successfully after treatment with plasmapheresis, methylprednisolone and prednisone. Conclusion This patient, whose serum was negative for the anti-GBM antibody but positive for p-ANCA and another atypical ANCA, had a rare case of anti-GBM disease. Insights from this unusual case might help physicians diagnose rare forms of glomerulonephritis and treat affected patients in a timely manner.

Three Years of Experience With Random Urinary Homovanillic and Vanillylmandelic Acid Levels in the Diagnosis of Neuroblastoma

PEDIATRICS ◽  
1987 ◽  
Vol 79 (2) ◽  
pp. 203-205
Author(s):  
Mendel Tuchman ◽  
Margaret L. R. Ramnaraine ◽  
William G. Woods ◽  
William Krivit
Keyword(s):  
False Positive ◽  
Homovanillic Acid ◽  
False Negative ◽  
False Negative Rate ◽  
Urine Samples ◽  
Vanillylmandelic Acid ◽  
Test Results ◽  
Upper Limit ◽  
Positive Results ◽  
Rule Out

During the last 3 years, random urine samples from 408 patients were tested for elevated homovanillic acid (HVA) and vanillylmandelic acid (VMA) levels to rule out the diagnosis of neuroblastoma. Thirty-seven of these patients had elevated HVA and/or VMA levels, and neuroblastoma was subsequently diagnosed. In three additional patients with negative test results (normal HVA and VMA levels), tumors were subsequently diagnosed (false-negative rate of 7.5%). Ten percent of the patients with neuroblastoma had normal HVA and 27.5% had normal VMA levels at the time of diagnosis. Only one patient (2.5%) with neuroblastoma had elevated VMA levels in the presence of normal HVA levels. More than 60% of the patients with neuroblastoma had urinary HVA and/or VMA levels higher than twice the upper limit of normal. No false-positive results were encountered. Age and stage distributions of the patients are shown, and the significance of the results is discussed.

Pediatric reference interval verification for endocrine and fertility hormone assays on the Abbott Alinity system

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Mary Kathryn Bohn ◽  
Siobhan Wilson ◽  
Alexandra Hall ◽  
Khosrow Adeli
Keyword(s):  
Clinical Decision Making ◽  
De Novo ◽  
Reference Interval ◽  
Clinical Decision ◽  
Reference Intervals ◽  
Healthy Children ◽  
Confidence Limits ◽  
Test Results ◽  
Serum Samples ◽  
Abbott Architect

Abstract Objectives The Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) has developed an extensive database of reference intervals (RIs) for several biomarkers on various analytical systems. In this study, pediatric RIs were verified for key immunoassays on the Abbott Alinity system based on the analysis of healthy children samples and comparison to comprehensive RIs previously established for Abbott ARCHITECT assays. Methods Analytical performance of Alinity immunoassays was first assessed. Subsequently, 100 serum samples from healthy children recruited with informed consent were analyzed for 16 Alinity immunoassays. The percentage of test results falling within published CALIPER ARCHITECT reference and confidence limits was determined. If ≥ 90% of test results fell within the confidence limits, they were considered verified based on CLSI guidelines. If <90% of test results fell within the confidence limits, additional samples were analyzed and new Alinity RIs were established. Results Of the 16 immunoassays assessed, 13 met the criteria for verification with test results from ≥ 90% of healthy serum samples falling within the published ARCHITECT confidence limits. New CALIPER RIs were established for free thyroxine and prolactin on the Alinity system. Estradiol required special considerations in early life. Conclusions Our data demonstrate excellent concordance between ARCHITECT and Alinity immunoassays, as well as the robustness of previously established CALIPER RIs for most immunoassays, eliminating the need for de novo RI studies for most parameters. Availability of pediatric RIs for immunoassays on the Alinity system will assist clinical laboratories using this new platform and contribute to improved clinical decision-making.

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