scholarly journals Alkaline post-incubation improves the saccharification of poplar after hydrogen peroxide–acetic acid pretreatment

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Peiyao Wen ◽  
Ying Zhang ◽  
Junjun Zhu ◽  
Yong Xu ◽  
Junhua Zhang
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Enzymatic Hydrolysis ◽  
High Concentration ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
Selective Delignification ◽  
Acetyl Group ◽  
The Cost ◽  
Total Lignin

Abstract Background Hydrogen peroxide–acetic acid (HPAA) is widely used in pretreatment of lignocellulose because it has a good capability in selective delignification. However, high concentration (more than 60%) of HPAA increases the cost of pretreatment and the risk of explosion. In this work, alkaline post-incubation was employed to decrease the HPAA loading and improve the saccharification of poplar. Results Pretreatment with 100% HPAA removed 91.0% lignin and retained 89.9% glucan in poplar. After poplar was pretreated by 100% HPAA at 60 °C for 2 h, the glucan conversion in enzymatic hydrolysis by cellulase increased to 90.1%. Alkaline incubation reduced the total lignin, surface lignin, and acetyl group of HPAA-pretreated poplar. More than 92% acetyl groups of HPAA-pretreated poplar were removed by alkaline incubation with 1.0% NaOH at 50 °C for 1 h. After incubation of 60% HPAA-pretreated poplar with 1.0% NaOH, the glucan conversion enhanced to 95.0%. About 40% HPAA loading in pretreatment was reduced by alkaline incubation without the decrease of glucose yield. Conclusions Alkaline post-incubation had strong ability on the deacetylation and delignification of HPAA-pretreated poplar, exhibiting a strong promotion on the enzymatic hydrolysis yield. This report represented alkaline incubation reduced the HPAA loading, improved pretreatment safety, exhibiting excellent potential application in saccharification of poplar.

scholarly journals Evaluation of the Interactive Effect Pretreatment Parameters via Three Types of Microwave-Assisted Pretreatment and Enzymatic Hydrolysis on Sugar Yield

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 787 ◽  
Author(s):  
Saleem Ethaib ◽  
Rozita Omar ◽  
Mustapa Kamal Siti Mazlina ◽  
Awang Biak Dayang Radiah
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Interactive Effect ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Solid Loading ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
Sago Palm ◽  
Microwave Assisted

This study aims to evaluate the sugar yield from enzymatic hydrolysis and the interactive effect pretreatment parameters of microwave-assisted pretreatment on glucose and xylose. Three types of microwave-assisted pretreatments of sago palm bark (SPB) were conducted for enzymatic hydrolysis, namely: microwave-sulphuric acid pretreatment (MSA), microwave-sodium hydroxide pretreatment (MSH), and microwave-sodium bicarbonate (MSB). The experimental design was done using a response surface methodology (RSM) and Box–Behenken Design (BBD). The pretreatment parameters ranged from 5–15% solid loading (SL), 5–15 min of exposure time (ET), and 80–800 W of microwave power (MP). The results indicated that the maximum total reducing sugar was 386 mg/g, obtained by MSA pretreatment. The results also illustrated that the higher glucose yield, 44.3 mg/g, was found using MSH pretreatment, while the higher xylose yield, 43.1 mg/g, resulted from MSA pretreatment. The pretreatment parameters MP, ET, and SL showed different patterns of influence on glucose and xylose yield via enzymatic hydrolysis for MSA, MSH, and MSB pretreatments. The analyses of the interactive effect of the pretreatment parameters MP, ET, and SL on the glucose yield from SPB showed that it increased with the high MP and longer ET, but this was limited by low SL values. However, the analysis of the interactive effect of the pretreatment parameters on xylose yields revealed that MP had the most influence on the xylose yield for MSA, MSH, and MSB pretreatments.

Hydrogen peroxide-acetic acid pretreatment increases the saccharification and enzyme adsorption on lignocellulose

2019 ◽  
Vol 140 ◽  
pp. 111657 ◽  
Author(s):  
Thatiane R. Mota ◽  
Dyoni M. Oliveira ◽  
Gutierrez R. Morais ◽  
Rogério Marchiosi ◽  
Marcos S. Buckeridge ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Enzyme Adsorption ◽  
Acid Pretreatment

scholarly journals Nitric Acid Pretreatment of Jerusalem Artichoke Stalks for Enzymatic Saccharification and Bioethanol Production

Energies ◽  
2018 ◽  
Vol 11 (8) ◽  
pp. 2153 ◽  
Author(s):  
Urszula Dziekońska-Kubczak ◽  
Joanna Berłowska ◽  
Piotr Dziugan ◽  
Piotr Patelski ◽  
Katarzyna Pielech-Przybylska ◽  
...  
Keyword(s):  
Nitric Acid ◽  
Enzymatic Hydrolysis ◽  
High Efficiency ◽  
Ethanol Yield ◽  
Enzymatic Saccharification ◽  
Jerusalem Artichoke ◽  
Potential Candidate ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
Theoretical Yield

This paper evaluated the effectiveness of nitric acid pretreatment on the hydrolysis and subsequent fermentation of Jerusalem artichoke stalks (JAS). Jerusalem artichoke is considered a potential candidate for producing bioethanol due to its low soil and climate requirements, and high biomass yield. However, its stalks have a complexed lignocellulosic structure, so appropriate pretreatment is necessary prior to enzymatic hydrolysis, to enhance the amount of sugar that can be obtained. Nitric acid is a promising catalyst for the pretreatment of lignocellulosic biomass due to the high efficiency with which it removes hemicelluloses. Nitric acid was found to be the most effective catalyst of JAS biomass. A higher concentration of glucose and ethanol was achieved after hydrolysis and fermentation of 5% (w/v) HNO3-pretreated JAS, leading to 38.5 g/L of glucose after saccharification, which corresponds to 89% of theoretical enzymatic hydrolysis yield, and 9.5 g/L of ethanol. However, after fermentation there was still a significant amount of glucose in the medium. In comparison to more commonly used acids (H2SO4 and HCl) and alkalis (NaOH and KOH), glucose yield (% of theoretical yield) was approximately 47–74% higher with HNO3. The fermentation of 5% nitric-acid pretreated hydrolysates with the absence of solid residues, led to an increase in ethanol yield by almost 30%, reaching 77–82% of theoretical yield.

Optimization of Dilute Acid Hydrolysis of Corn Stover for Separate Production of Xylose and Glucose by Response Surface Methodology

2012 ◽  
Vol 608-609 ◽  
pp. 298-301
Author(s):  
Su Li Zhi ◽  
Jing Yang ◽  
Yan Yao ◽  
Shu Ting Zhang ◽  
Xue Bin Lu
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Corn Stover ◽  
High Concentration ◽  
Dilute Acid Hydrolysis ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
H2so4 Concentration ◽  
Glucose Ratio ◽  
Dilute Sulfuric Acid Pretreatment

Dilute sulfuric acid pretreatment of corn stover was used to obtain a solution of high concentration of xylose from the hemicellulosic fraction and a relatively low concentration of glucose, which not only saved the hemicellulase but also made a full use of corn stover. Then the study considered the selectivity (xylose-glucose ratio) as an important parameter to optimize the hydrolysis conditions. The results optimized by response surface methodology (RSM) showed that the optimum conditions for pretreatment were found to be H2SO4 concentration of 2.4% and reaction time of 100min at 100°C. Under these conditions, 78.8% of xylose yield was achieved and the glucose yield was lower than 11.6%. To confirm these results, the optimum condition was performed and the actual results of xylose yield and glucose yield were 78% and 11.3%.

Production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic acid-pretreated poplar by two-step enzymatic hydrolysis

2020 ◽  
Vol 297 ◽  
pp. 122349 ◽  
Author(s):  
Xixun Hao ◽  
Peiyao Wen ◽  
Jia Wang ◽  
Jinye Wang ◽  
Jiaxin You ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Enzymatic Hydrolysis

Peroxide−Acetic Acid Pretreatment To Remove Bagasse Lignin Prior to Enzymatic Hydrolysis

2010 ◽  
Vol 49 (4) ◽  
pp. 1473-1479 ◽  
Author(s):  
He Tan ◽  
Rallming Yang ◽  
Weidong Sun ◽  
Shuangfei Wang
Keyword(s):  
Acetic Acid ◽  
Enzymatic Hydrolysis ◽  
Acid Pretreatment

scholarly journals Rice Straws With Different Cell Wall Components Differ on Abilities of Saccharification

2021 ◽  
Vol 8 ◽  
Author(s):  
Chen Chen ◽  
Xiaoxiao Deng ◽  
Weilong Kong ◽  
Mirza Faisal Qaseem ◽  
Shuai Zhao ◽  
...  
Keyword(s):  
Cell Wall ◽  
Energy Use ◽  
Treatment Strategies ◽  
Wall Structure ◽  
High Concentration ◽  
Acid Pretreatment ◽  
Rice Varieties ◽  
Glucose Yield ◽  
High Acid ◽  
High Alkali

Rice straw has an enormous amount of biomass for energy use, but the complexity of the cell wall component hinders technical processes. Although belonging to rice straws, the straws from different varieties should be with different treatment strategies to obtain best energy efficiency. To confirm this hypothesis, 7 different rice varieties (RPY GENG, RIL269, RIL272, RIL31, RIL57, RIL06, LUOHUI 9) with different cell wall traits from RIL population were evaluated for their response toward different pretreatments. For japonica RPY GENG, 2% of H2SO4 acid was best pre-treatment while high acid (5% of H2SO4) pretreatment caused undue loss. For Indica LUOHUI 9 rice, high acid pretreatment was suitable, while RIL57 had maximum of glucose yield with high alkali (10% NaOH) pretreatment. High-concentration alkali pretreatment is the most convenient and effective pretreatment method for the treatment of unknown varieties of rice straws, because the lignin has been removed and has the lowest negative effects on the glucose yield under the high alkali condition. As the RILs used in this study vary considerably in their wall structure, an understanding of their response to different pre-treatments confirms our hypothesis and help us to understand the influence of different wall compositions on the final output.

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