Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440

Abstract Purpose Pseudomonas putida KT2440 is a desiccation-sensitive bacterium that loses culturability after 15 days of air desiccation. We have previously shown that P. putida KT2440 can develop a viable but nonculturable (VBNC) state after being exposed to desiccation stress and eventually recover when desiccated cells are rehydrated for at least 24 h. Methods To determine which genes of transport, oxidation-reduction, and transcription processes could be involved in the return of P. putida KT2440 to the culturable state, a transcriptome analysis was carried out comparing the gene expression of non-desiccated samples with samples subjected to desiccation followed by 20 min of rehydration or desiccation followed by 24 h of rehydration. Results Desiccation stress triggered a VBNC state of P. putida. The major response was detected after 24 h of rehydration with 148 upregulated and 42 downregulated genes. During the VBNC state, P. putida activated transmembrane transport processes like that of siderophores through a TonB-dependent transporter and putative polyhydric alcohol transport systems. Prolonged rehydration with distilled water resuscitated P. putida KT2440 cells activating the catabolism of phenylalanine/tyrosine to provide energy and carbon for ubiquinone biosynthesis while maintaining a reduced protein synthesis. On the other hand, the interruption of the TonB-dependent receptor gene (PP_1446) increased desiccation survival of the mutant strain. Conclusion The activation of the iron transport system (TonB-dependent siderophore receptor) and alcohol transport can be helping the VBNC state of P. putida. Activation of catabolism of phenylalanine/tyrosine and reduced protein synthesis was needed for resuscitation from the VBNC state.

Download Full-text

The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440

10.1101/2020.08.24.265637 ◽

2020 ◽

Author(s):

Rafael Montenegro ◽

Sofía Vieto ◽

Daniela Wicki-Emmenegger ◽

Felipe Vásquez-Castro ◽

Carolina Coronado-Ruiz ◽

...

Keyword(s):

Pseudomonas Putida ◽

Type Strain ◽

Wild Type Strain ◽

Electronic Waste ◽

Chemical Species ◽

Growth Curves ◽

Phosphate Transporter ◽

Transport Systems ◽

Wild Type ◽

Pseudomonas Putida Kt2440

AbstractTellurium oxyanions are chemical species with great toxicity; their presence in the environment has increased because of mining industries and photovoltaic and electronic waste. Recovery strategies based on microorganisms for this metalloid are of interest, but further studies of the transport systems and enzymes responsible for implementing tellurium transformations are required because many mechanisms remain unknown. Here, we investigated the involvement in tellurite uptake of the putative phosphate transporter PitB (PP1373) in soil bacterium Pseudomonas putida KT2440. For this purpose, through a method based on the CRISPR/Cas9 system, we generated a strain deficient in pitB gene and characterized its phenotype on exposing it to varied concentrations of tellurite. Growth curves and Transmission Electronic Microscopy experiments of wild type and ΔpitB showed that both strains were able to internalize tellurite into the cytoplasm and reduce the oxyanion to black nano-sized and rod-shaped tellurium particles, however, ΔpitB strain showed an increased resistance to the tellurite toxic effects. At a concentration of 100 uM tellurite, where the biomass formation of wild type strain decreased by half, we observed a greater ability of ΔpitB to reduce this oxyanion with respect to wild type strain (~38% vs ~16%), which is related by the greater biomass production of ΔpitB and not by a greater consumption of tellurite per cell. The phenotype of the mutant was restored on over-expressing pitB in trans. In summary, our results indicate that PitB is one of several transporters responsible for tellurite uptake in P. putida KT2440.

Download Full-text

The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440

Microbiology ◽

10.1099/mic.0.001002 ◽

2020 ◽

Author(s):

Rafael Montenegro ◽

Sofía Vieto ◽

Daniela Wicki-Emmenegger ◽

Felipe Vásquez-Castro ◽

Carolina Coronado-Ruiz ◽

...

Keyword(s):

Pseudomonas Putida ◽

Type Strain ◽

Type Species ◽

Wild Type Strain ◽

Phosphate Transporter ◽

Transport Systems ◽

Wild Type ◽

Pseudomonas Putida Kt2440 ◽

Content Type ◽

Link Type

Tellurium oxyanions are chemical species of great toxicity and their presence in the environment has increased because of mining industries and photovoltaic and electronic waste. Recovery strategies for this metalloid that are based on micro-organisms are of interest, but further studies of the transport systems and enzymes responsible for implementing tellurium transformations are required because many mechanisms remain unknown. Here, we investigated the involvement in tellurite uptake of the putative phosphate transporter PitB (PP1373) in soil bacterium Pseudomonas putida KT2440. For this purpose, through a method based on the CRISPR/Cas9 system, we generated a strain deficient in the pitB gene and characterized its phenotype on exposing it to varied concentrations of tellurite. Growth curves and transmission electronic microscopy experiments for the wild-type and ΔpitB strains showed that both were able to internalize tellurite into the cytoplasm and reduce the oxyanion to black nano-sized and rod-shaped tellurium particles, although the ΔpitB strain showed an increased resistance to the tellurite toxic effects. At a concentration of 100 μM tellurite, where the biomass formation of the wild-type strain decreased by half, we observed a greater ability of ΔpitB to reduce this oxyanion with respect to the wild-type strain (~38 vs ~16 %), which is related to the greater biomass production of ΔpitB and not to a greater consumption of tellurite per cell. The phenotype of the mutant was restored on over-expressing pitB in trans. In summary, our results indicate that PitB is one of several transporters responsible for tellurite uptake in P. putida KT2440.

Download Full-text

Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440

Metabolic Engineering ◽

10.1016/j.ymben.2021.07.005 ◽

2021 ◽

Author(s):

Allison Z. Werner ◽

Rita Clare ◽

Thomas D. Mand ◽

Isabel Pardo ◽

Kelsey J. Ramirez ◽

...

Keyword(s):

Pseudomonas Putida ◽

Ethylene Terephthalate ◽

Pseudomonas Putida Kt2440 ◽

Poly Ethylene Terephthalate ◽

Poly Ethylene

Download Full-text

Disruption of the siderophore-binding desE receptor gene in Streptomyces coelicolor A3(2) results in impaired growth in spite of multiple iron-siderophore transport systems

Microbial Biotechnology ◽

10.1111/j.1751-7915.2010.00240.x ◽

2011 ◽

Vol 4 (2) ◽

pp. 275-285 ◽

Cited By ~ 21

Author(s):

Víctor H. Tierrafría ◽

Hilda E. Ramos-Aboites ◽

Guillermo Gosset ◽

Francisco Barona-Gómez

Keyword(s):

Streptomyces Coelicolor ◽

Receptor Gene ◽

Transport Systems ◽

Siderophore Transport

Download Full-text

A Broad Spectrum Racemase in Pseudomonas putida KT2440 Plays a Key Role in Amino Acid Catabolism

Frontiers in Microbiology ◽

10.3389/fmicb.2018.01343 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 2

Author(s):

Atanas D. Radkov ◽

Luke A. Moe

Keyword(s):

Amino Acid ◽

Pseudomonas Putida ◽

Broad Spectrum ◽

Amino Acid Catabolism ◽

Pseudomonas Putida Kt2440

Download Full-text

Eliminating a global regulator of carbon catabolite repression enhances the conversion of aromatic lignin monomers to muconate in Pseudomonas putida KT2440

Metabolic Engineering Communications ◽

10.1016/j.meteno.2017.05.002 ◽

2017 ◽

Vol 5 ◽

pp. 19-25 ◽

Cited By ~ 42

Author(s):

Christopher W. Johnson ◽

Paul E. Abraham ◽

Jeffrey G. Linger ◽

Payal Khanna ◽

Robert L. Hettich ◽

...

Keyword(s):

Pseudomonas Putida ◽

Catabolite Repression ◽

Carbon Catabolite Repression ◽

Global Regulator ◽

Pseudomonas Putida Kt2440

Download Full-text

Transport Phenomena and Properties in Treelike Networks

Applied Mechanics Reviews ◽

10.1115/1.4033966 ◽

2016 ◽

Vol 68 (4) ◽

Cited By ~ 47

Author(s):

Peng Xu ◽

Agus Pulung Sasmito ◽

Boming Yu ◽

Arun Sadashiv Mujumdar

Keyword(s):

Transport Properties ◽

Scaling Laws ◽

Transport Phenomena ◽

Transport Processes ◽

Research Progress ◽

Transport Systems ◽

Transport Mechanisms ◽

Branching Structures ◽

Flow And Heat Transfer ◽

Flow Heat

Treelike structures abound in natural as well as man-made transport systems, which have fascinated multidisciplinary researchers to study the transport phenomena and properties and understand the transport mechanisms of treelike structures for decades. The fluid flow and heat transfer in treelike networks have received an increasing attention over the past decade as the highly efficient transport processes observed in natural treelike structures can provide useful hints for optimal solutions to many engineering and industrial problems. This review paper attempts to present the background and research progress made in recent years on the transport phenomenon in treelike networks as well as technological applications of treelike structures. The subtopics included are optimization of branching structures, scaling laws of treelike networks, and transport properties for laminar flow, turbulent flow, heat conduction, and heat convection in treelike networks. Analytical expressions for the effective transport properties have been derived based on deterministic treelike networks, and the effect of branching parameters on the transport properties of treelike networks has also been discussed. Furthermore, numerical simulation results for treelike microchannel networks are presented as well. The proposed transport properties may be beneficial to understand the transport mechanisms of branching structures and promote the applications of treelike networks in engineering and industry.

Download Full-text