scholarly journals A high abundance of Firmicutes in the intestine of chinese mitten crabs (Eriocheir sinensis) cultured in an alkaline region

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Weibing Guan ◽  
Kui Li ◽  
Shuang Zhao ◽  
Kejun Li
Keyword(s):  
Alpha Diversity ◽  
Illumina Miseq ◽  
Northwest China ◽  
Eriocheir Sinensis ◽  
Pond Aquaculture ◽  
Alkaline Region ◽  
Intestinal Bacterial Community ◽  
Mitten Crab ◽  
Mitten Crabs ◽  
Length Width

AbstractThe Chinese mitten crab (Eriocheir sinensis) is a popular aquaculture product in East Asia, especially in China. In the last decade, rice–crab co-culture has rapidly expanded in China. Under this model, crabs are raised in rice fields instead of in traditional aquaculture ponds. In this study, we cultured two varieties of Chinese mitten crabs (Changjiang and Liaohe) in an alkaline region in northwest China and used Illumina MiSeq sequencing to compare the intestinal bacterial alpha diversity and community structure between traditional and co-culture aquaculture models, between two crab varieties, and between female and male crabs. Significant variations in intestinal bacterial communities were found between crab varieties and between female and male crabs but not between aquaculture models. These results show that rice–crab co-culture operations did not obviously impact the crab intestinal bacterial community compared with traditional pond aquaculture. Firmicutes was the most abundant bacterial phylum in the crab intestines (78%, relative abundance). Three dominant operational taxonomic units (OTUs) represented 73.2% of Firmicutes sequences and 56.8% of all sequences. A dominant OTU assigned as Firmicutes that was negatively correlated with crab body length, width, and weight was found in the source water for the experimental area. The results of this study suggest that the aquaculture of Chinese mitten crabs in alkaline regions requires more study to improve cultivation techniques.

Can morphological characters be used to identify the pond-reared offspring of wild Chinese mitten crabs, Eriocheir sinensis, from different basins?

Crustaceana ◽  
2019 ◽  
Vol 92 (8) ◽  
pp. 957-978
Author(s):  
Xiaodong Jiang ◽  
Xugan Wu ◽  
Haining Wang ◽  
Yuhong Yang ◽  
Yongxu Cheng
Keyword(s):  
Morphological Difference ◽  
Principal Component ◽  
Morphological Characters ◽  
Eriocheir Sinensis ◽  
Stepwise Discriminant Analysis ◽  
Chinese Mitten Crab ◽  
Artificial Culture ◽  
Mitten Crab ◽  
Mitten Crabs ◽  
Morphological Differences

Abstract Although morphology is always used to distinguish wild Chinese mitten crab (Eriocheir sinensis) populations, whether morphology could be used to identify the pond-reared offspring of wild E. sinensis populations has remained unclear. This study was undertaken to examine the morphological difference of wild E. sinensis (G0) from the Yangtze River (YR), Huang River (HR) and Liao River (LR) as well as their pond-reared offspring (G1). The results showed that: (1) The amount of significantly different parameters of three G1 populations was evidently less than that of three G0 populations; (2) G0 and G1 individuals of each population were clearly separated by principal component analysis, furthermore, three G0 populations were clearly separated while three G1 populations were not well distinguished from each other; (3) discrimination accuracy of G1 individuals in stepwise discriminant analysis was obviously lower than that of G1 populations; (4) G0 and G1 populations were clustered into two separate clusters in the cluster analysis. In conclusion, morphological differences between wild E. sinensis populations had disappeared after one generation of artificial culture, and morphology thus probably cannot be used to distinguish the pond-reared offspring originated from three wild E. sinensis populations.

scholarly journals Anthropogenic CO<sub>2</sub>-mediated freshwater acidification limits survival, calcification, metabolism, and behaviour in stress-tolerant freshwater crustaceans

2021 ◽  
Vol 18 (23) ◽  
pp. 6287-6300
Author(s):  
Alex R. Quijada-Rodriguez ◽  
Pou-Long Kuan ◽  
Po-Hsuan Sung ◽  
Mao-Ting Hsu ◽  
Garett J. P. Allen ◽  
...  
Keyword(s):  
Eriocheir Sinensis ◽  
Integrative Approach ◽  
Acid Base ◽  
Trade Offs ◽  
Mitten Crab ◽  
Mitten Crabs ◽  
Freshwater Acidification ◽  
Locomotory Behaviour ◽  
The Cost ◽  
The Impact

Abstract. Dissolution of anthropogenic CO2 is chronically acidifying aquatic ecosystems. Studies indicate that ocean acidification will cause marine life, especially calcifying species, to suffer at the organism and ecosystem levels. In comparison, freshwater acidification has received less attention, rendering its consequences unclear. Here, juvenile Chinese mitten crabs, Eriocheir sinensis, were used as a crustacean model to investigate the impact of CO2-mediated freshwater acidification. Our integrative approach, investigating changes in the animal's acid–base homeostasis, metabolism, calcification, locomotory behaviour, and survival rate, indicates that this economically relevant crustacean will face energetic consequences from future freshwater acidification. These energetic trade-offs allow the animal to maintain its acid–base homeostasis at the cost of reduced metabolic activity, exoskeletal calcification, and locomotion, reducing the animal's overall fitness and increasing its mortality. Results indicate that present-day Chinese mitten crab could be heavily affected by freshwater acidification like their marine counterparts and emphasize the importance of understanding the long-term implications of freshwater acidification on species' fitness.

scholarly journals Chinese Mitten Crabs (Eriocheir Sinensis) Could Act As Vehicles of Spreading Avian Influenza Virus

2021 ◽  
Author(s):  
Weiwei Ma ◽  
Chenyang Ren ◽  
Qingbiao Hu ◽  
Xiaodong Li ◽  
Yali Feng ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Significant Risk ◽  
Eriocheir Sinensis ◽  
Contaminated Water ◽  
Surrounding Water ◽  
Biotic Factor ◽  
Mitten Crab ◽  
Mitten Crabs

Abstract Avian influenza virus (AIV) possessed significant risk to various animals and human health. Wild birds, especially waterfowls are considered to be the natural reservoir of AIVs. The ecology of AIV is still far from being fully understood. Chinese mitten crabs are nonnegligible biotic factor in AIV ecosystem. We analyzed the ability of Chinese mitten crabs accumulate and spread AIV. We found that AIV remain infectious in water only for 36 hours but persist in crabs for 48 hours. Crabs grills and gastrointestinal tracts accumulated AIV with higher titers than viral water. Crabs could accumulate AIV from contaminated water, carry the virus and spread to naïve crabs via surrounding water. Our study identified Chinese mitten crab as a novel transmission vehicle in AIV ecosystem.

scholarly journals Divergence in Gut Bacterial Community Structure between Male and Female Stag Beetles Odontolabis fallaciosa (Coleoptera, Lucanidae)

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2352
Author(s):  
Xia Wan ◽  
Yu Jiang ◽  
Yuyan Cao ◽  
Binghua Sun ◽  
Xingjia Xiang
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
High Throughput Sequencing ◽  
Alpha Diversity ◽  
Illumina Miseq ◽  
Bacterial Interactions ◽  
Intestinal Bacterial Community ◽  
Males And Females ◽  
Stag Beetles

Odontolabis fallaciosa (Coleoptera: Lucanidae) is a giant and popular stag beetle with striking sexual dimorphism and male trimorphism. However, little is known about their intestinal microbiota, which might play an indispensable role in shaping the health of their hosts. The aim of this study was to investigate the intestinal bacterial community structure between the two sexes and among three male morphs of O. fallaciosa from China using high-throughput sequencing (Illumina MiSeq). The gut bacterial community structure was significantly different between males and females, suggesting that sex appeared to be the crucial factor shaping the intestinal bacterial community. Females had higher bacterial alpha-diversity than males. There was little difference in gut bacterial community structure among the three male morphs. However, compared to medium and small males, large individuals were associated with the higher relative abundance of Firmicutes and Firmicutes/Bacteroides (F/B) ratio, which might contribute to nutritional efficiency. Overall, these results might help to further our understanding of beetle–bacterial interactions of O. fallaciosa between the two sexes, and among the three male morphs.

Mechanics of Locomotion Energetics in Chinese Mitten Crab Eriocheir sinensis Milne-Edwards

2013 ◽  
Vol 461 ◽  
pp. 247-253
Author(s):  
Xiao Dong Zhang ◽  
Jian Qiao Li ◽  
Han Huang ◽  
Meng Zou
Keyword(s):  
Energy Recovery ◽  
High Speed ◽  
Mechanical Energy ◽  
Video Recording ◽  
Gait Pattern ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Flat Terrain ◽  
Mitten Crab ◽  
Mitten Crabs

The study on the locomotion mechanism in laboratory has defined performance limits for animals presently. But it is more significant for investigating mechanics of animals in their free state. In order to study the locomotion properties of Chinese mitten crabs Eriocheir sinensis Milne-Edwards on one flat terrain and four kinds of rough terrains, a high speed 3-D video recording system was used to record motion video images of crabs. The gait pattern, average speeds, the mechanical energy of the mass center and percentage energy recovery were investigated with motion analysis system. The results showed that Chinese mitten crabs used alternating tetrapod gait on flat terrain and with increasing of terrain roughness, the regularity of gait tend to be less conspicuous. Crabs used two fundamental models of energy exchanging patterns: the inverted pendulum gait and the bouncing gait and the bouncing gait was the main energy saving and conserving pattern. Keywords-biomechanics, Chinese mitten crab, rough terrain, gait, mechanical energy, percentage energy recovery

Prevalence and Potential Pathogenicity of Vibrio parahaemolyticus in Chinese Mitten Crabs (Eriocheir sinensis) Harvested from the River Thames Estuary, England

2009 ◽  
Vol 72 (1) ◽  
pp. 60-66 ◽  
Author(s):  
SARIQA WAGLEY ◽  
KEGAKILWE KOOFHETHILE ◽  
RACHEL RANGDALE
Keyword(s):  
United Kingdom ◽  
Vibrio Parahaemolyticus ◽  
Eriocheir Sinensis ◽  
Alien Invasive Species ◽  
Physical Damage ◽  
Human Pathogenic Bacterium ◽  
Mitten Crab ◽  
Mitten Crabs ◽  
Thermostable Direct Hemolysin ◽  
River Thames

Chinese mitten crabs (Eriocheir sinensis) have been described as an alien invasive species in the River Thames, United Kingdom, and elsewhere in Europe. The crabs can cause considerable physical damage to the riverbeds and threaten native ecosystems. Trapping has been considered an option, but such attempts to control mitten crab populations in Germany in the 1930s failed. In the United Kingdom, it has been suggested that commercial exploitation of the species could be employed as a control option. This study was conducted as part of a larger program to assess the suitability of a commercial Chinese mitten crab fishery in the River Thames. Crabs and water samples from the River Thames between 2003 and 2006 were examined for the human pathogenic bacterium Vibrio parahaemolyticus. All samples throughout this testing period were positive for V. parahaemolyticus. The putative pathogenicity markers, thermostable direct hemolysin and thermostable direct–related hemolysin, were detected in one sample, indicating that the crabs possessed the potential to cause V. parahaemolyticus–associated illness if consumed without further processing. Levels of V. parahaemolyticus were higher during the summer than in the winter. This is the first study of V. parahaemolyticus prevalence in European-adapted Chinese mitten crabs.

Changes in condition and in carapace length and width of the Chinese mitten crab (Eriocheir sinensis H. Milne Edwards, 1853) harvested in the Odra River estuary in 1999-2007

2010 ◽  
Vol 39 (2) ◽  
Author(s):  
Przemysław Czerniejewski
Keyword(s):  
Carapace Length ◽  
River Estuary ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Mitten Crabs ◽  
Odra River

Changes in condition and in carapace length and width of the Chinese mitten crab (Carapace length and width as well as condition were analysed in 2336 Chinese mitten crabs,

scholarly journals Role of Transportome in the Gills of Chinese Mitten Crabs in Response to Salinity Change: A Meta-Analysis of RNA-Seq Datasets

Biology ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 39
Author(s):  
Adeel Malik ◽  
Chang-Bae Kim
Keyword(s):  
Meta Analysis ◽  
Eriocheir Sinensis ◽  
Rna Seq ◽  
Salinity Change ◽  
Important Species ◽  
Freshwater Aquaculture ◽  
Mitten Crab ◽  
Mitten Crabs ◽  
Selection Of

Chinese mitten crab (CMC) or Eriocheir sinensis is a strong osmoregulator that can keep rigorous cellular homeostasis. CMC can flourish in freshwater, as well as seawater, habitats and represents the most important species for freshwater aquaculture. Salt stress can have direct effects on several stages (e.g., reproduction, molting, growth, etc.) of the CMC life cycle. To get a better overview of the genes involved in the gills of CMC under different salinity conditions, we conducted an RNA-Seq meta-analysis on the transcriptomes of four publicly available datasets. The meta-analysis identified 405 differentially expressed transcripts (DETs), of which 40% were classified into various transporter classes, including accessory factors and primary active transporters as the major transport classes. A network analysis of the DETs revealed that adaptation to salinity is a highly regulated mechanism in which different functional modules play essential roles. To the best of our knowledge, this study is the first to conduct a transcriptome meta-analysis of gills from crab RNA-Seq datasets under salinity. Additionally, this study is also the first to focus on the differential expression of diverse transporters and channels (transportome) in CMC. Our meta-analysis opens new avenues for a better understanding of the osmoregulation mechanism and the selection of potential transporters associated with salinity change.

scholarly journals First indication of Japanese mitten crabs in Europe and cryptic genetic diversity of invasive Chinese mitten crabs

NeoBiota ◽  
2019 ◽  
Vol 50 ◽  
pp. 1-29 ◽  
Author(s):  
Sarah Hayer ◽  
Dirk Brandis ◽  
Günther B. Hartl ◽  
Christine Ewers-Saucedo
Keyword(s):  
Genetic Diversity ◽  
Sequence Data ◽  
Eriocheir Sinensis ◽  
Source Population ◽  
Rapid Adaptation ◽  
Chinese Mitten Crab ◽  
Introduced Range ◽  
Mitten Crab ◽  
Mitten Crabs ◽  
Coi Sequences

The Chinese mitten crab (Eriocheir sinensis) is a prominent aquatic invader with substantial negative economic and environmental impacts. The aim of the present study was to re-evaluate the genetic diversity of mitten crabs throughout their native and invaded ranges based on publicly available sequence data, and assess if multiple introductions or rapid adaptation could be responsible for biologically divergent mitten crabs in Northern Europe. We assembled available genetic data of a fragment of the mitochondrial cytochrome c oxidase subunit one gene (COI) for all species of the genus Eriocheir. We applied phylogenetic and population genetic analyses to compare native and invasive populations, and to identify possible source populations. The phylogenetic reconstruction revealed that five COI sequences from Europe, morphologically identified as Chinese mitten crab, actually belong to the Japanese mitten crab (Eriocheir japonica), representing the first indication of its presence in European waters. All other COI sequences from Europe could unambiguously be assigned to the Chinese mitten crab. In some Northern German populations of Chinese mitten crabs, genetic diversity was surprisingly high, due to seven unique haplotypes encoding several amino acid substitutions. This diversity may reflect a cryptic introduction from an unsampled native location, or rapid adaptation in the invaded range. Based on the genetic diversity shared between native and introduced range, Feiyunjiang, a tributary of the Yangtze River, emerges as a plausible source population for the original introduction of Chinese mitten crabs to Europe. This study highlights the complex and dynamic invasion processes of mitten crabs in Europe. We urge to further monitor mitten crab invasions using genetic tools.

Sign in / Sign up

Export Citation Format

Share Document