scholarly journals Determination of antioxidant activity and phenolic compounds for basic standardization of Turkish propolis

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Aslı Özkök ◽  
Merve Keskin ◽  
Aslı Elif Tanuğur Samancı ◽  
Elif Yorulmaz Önder ◽  
Çiğdem Takma
Keyword(s):  
Antioxidant Capacity ◽  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Total Phenolic ◽  
Scaling Analysis ◽  
Flavonoid Content ◽  
Multidimensional Scaling Analysis ◽  
Flavonoid Quercetin ◽  
Phenolic And Flavonoid

AbstractThis study aimed to determine the standard amount of antioxidant content and compounds of the propolis for the standardization of propolis. For this purpose, the total flavonoids, total phenolic, CUPRAC antioxidant capacity content and the diversity of phenolic and flavonoid components of these propolis samples were found by HPLC determined at the 23 propolis samples which were collected different regions of Turkey. Beside that, the similarities and differences of these 23 provinces to each other according to their antioxidant capacities were investigated by multidimensional scaling analysis. The total flavonoid content in the propolis samples were determined between 21.28 and 152.56 mg CE/g. The total phenolic content in the propolis samples was found between 34.53 mg and 259.4 mg GAE/g. CUPRAC antioxidant capacity of the propolis samples and antioxidant range was found from 95.35 to 710.43 mg TE/g. Also, 4 flavonoid [Quercetin (min.1.12–max.4.14 mg/g), Galangin (min.0.72–max.40.79 mg/g), Apigenin (min.1.07–max.17.35 mg/g), Pinocembrin (min.1.32–max.39.92 mg/g] and 6 phenolic acid [Caffeic acid (min.1.20–max.7.6 mg/g), p-Coumaric acid (min.1.26–max.4.47 mg/g), trans-Ferulic acid (min.1.28–max.4.92 mg/g), Protocatechuic acid (1.78 mg/g), trans-Cinnamic acid (min.1.05–max.3.83 mg/g), Caffeic Acid Phenethyl Ester (CAPE) (min.1.41–max.30.15 mg/g)] components were detected as mg/g, in different ratios in propolis samples collected from different regions. The feature of this study, so far, is to have the maximum number of samples representing the Turkish propolis, and so is thought to help to national and international propolis standard workings.

scholarly journals Establishing Relationship between Vitamins, Total Phenolic and Total Flavonoid Content and Antioxidant Activities in Various Honey Types

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4399
Author(s):  
Norhasnida Zawawi ◽  
Pei Juin Chong ◽  
Nurul Nadhirah Mohd Tom ◽  
Nurkhairina Solehah Saiful Anuar ◽  
Salma Malihah Mohammad ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Extrinsic Factors ◽  
Biochemical Compounds ◽  
Phenolic And Flavonoid

Honey is a well-known natural sweetener and is rich in natural antioxidants that prevent the occurrence of oxidative stress, which is responsible for many human diseases. Some of the biochemical compounds in honey that contribute to this property are vitamins and phenolic compounds such as phenolic acids and flavonoids. However, the extent to which these molecules contribute towards the antioxidant capacity in vitro is inconsistently reported, especially with the different analytical methods used, as well as other extrinsic factors that influence these molecules’ availability. Therefore, by reviewing recently published works correlating the vitamin, total phenolic, and flavonoid content in honey with its antioxidant activities in vitro, this paper will establish a relationship between these parameters. Based on the literature, vitamins do not contribute to honey’s antioxidant capacity; however, the content of phenolic acids and flavonoids has an impact on honey’s antioxidant activity.

Antimicrobial Potential and Determination of Total Phenolic and Flavonoid Content of Aerial Part Extracts of Ajuga bracteosa Wall ex. Benth

2018 ◽  
Vol 8 (3) ◽  
pp. 114-118
Author(s):  
Tabasum Ali ◽  
Amreen Naqash ◽  
Rukhsar Wadoo ◽  
Rumaisa Rashid ◽  
Ghulam Nabi Bader
Keyword(s):  
Aerial Part ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antimicrobial Potential ◽  
Phenolic And Flavonoid

scholarly journals Antioxidant, Total Phenolic and Flavonoid Content of Selected Nigerian Medicinal Plants

2015 ◽  
Vol 14 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Matthias Onyebuchi Agbo ◽  
Philip Felix Uzor ◽  
Uchenna Nneamaka Akazie Nneji ◽  
Chidozie Uzoma Eze Odurukwe ◽  
Uchenna Basilia Ogbatue ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

Plant phenolics and flavonoids play a great role in scavenging free radicals in the body and act as antioxidants. Thus their determination is sometimes nedded. Total antioxidant capacity, total phenolic and flavonoid contents of the extracts of 10 medicinal plants (Lochnera rosea, Allamanda cathartica, Asplenium platyneuron, Euphorbia prostrate, Baphia nitida, Crotolaria retusa, Zapoteca portoricensis, Platycerium bifurcatum, Mussaenda afzelii and Craterosiphon scandens) from 7 botanical families growing in the tropical rainforest of Nigeria were included in this study. The total antioxidant capacity of the extracts was assessed by using the phosphomolybdate method. The phenolic content was determined by using Folin-Ciocalteau assay, while the total flavonoid was determined by the aluminium chloride colorimetric assay. The results obtained showed that the total antioxidant capacity for all the extracts were in the range of 0.888 ± 0.75 to 0.938 ± 0.00 mg EAA/g. The results showed that E. prostrate, P. bifurcatum and A. platyneuron were found to be the richest source of phenolic (97.77 ± 0.77, 87.62 ± 1.22 and 82.33 ± 0.30 mg GAE/g) while B. nitida and M. afzelii had the least total phenolic content (11.67 ± 0.09 and11.18 ± 0.30 mg GAE/g). The highest total flavonoid content was revealed in P. bifurcatum (648.67 ± 12.3 mg QE) while M. afzelii also had the least total flavonoid content (3.67 ± 0.00 mg QE/g). The ratio of flavonoid to the phenolic in each extract was also determined to ascertain extracts that are rich in flavonoids.Dhaka Univ. J. Pharm. Sci. 14(1): 35-41, 2015 (June)

Identification of the functional food potency of Çalkama: A traditional recipe with edible Mediterranean wild greens from Turkish Cuisine

2021 ◽  
pp. 1-12
Author(s):  
Gokce Altin ◽  
Fatih Bildik ◽  
Kadriye Nur Kasapoğlu ◽  
Seda Genç ◽  
Mahmut Genç ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acid ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Traditional Foods ◽  
Foeniculum Vulgare ◽  
Allium Ampeloprasum ◽  
Total Antioxidant ◽  
Fast Liquid Chromatography ◽  
Phenolic And Flavonoid

BACKGROUND: The phytochemical contents of traditional foods are necessary to further elucidate the impacts of the Mediterranean diet on health. Çalkama is traditional food prepared by using wild green plants including chard (Beta vulgaris var.cicl), wild fennel (Foeniculum vulgare spp), common mellow (Malva sylvestris L), common poppy (Papaver rhoeas L), dock (Rumex spp), common nettle (Urtica dioica L), sow-thistle (Sonchus asper (L) Hill), common stork’s bill (Erodium circutarium (L)L Hér) and wild leek (Allium ampeloprasum L). OBJECTIVE: In this research, the antioxidant activity and the flavonoid and phenolic acid composition were separately analyzed for each plant and Çalkama. METHODS: Total phenolic content, total flavonoid content and total antioxidant capacity of each plant and Çalkama were measured spectrophotometric assays. Ultra-fast liquid chromatography (UFLC) was performed to detect specific flavonoid groups. RESULTS: It was detected that one portion of çalkama (100 g) contained approximately 250 mg of flavonoids. Particularly, quercetin and apigenin contributed to the main flavonoid source and chlorogenic acid was the major phenolic acid in çalkama. CONCLUSIONS: According to our findings, this food can be considered as a good phenolic and flavonoid source which protects its high antioxidant capacity through preparation and cooking processes.

scholarly journals Determination of Total Phenolic and Flavonoid Content in Small Berries

2021 ◽  
Vol 83 (9) ◽  
pp. 579-583
Author(s):  
Renata Bellová ◽  
Dana Blahútová ◽  
Eva Culková ◽  
Peter Kubatka ◽  
Peter Tomčík
Keyword(s):  
Vaccinium Corymbosum ◽  
Total Phenolic ◽  
Theoretical Knowledge ◽  
Flavonoid Content ◽  
Student Centered ◽  
Chemistry Teachers ◽  
Fundamental Part ◽  
Vaccinium Myrtillus L ◽  
Phenolic And Flavonoid

The authors of this article applied the determination of phenolic and flavonoid content in small berries to create a didactic cycle based on student-centered active learning (SCAL) in which students actively participated in the topic while studying and profile-forming as future biology and chemistry teachers. The fundamental part of the proposed didactic cycle is that students solve the following task: Determine the phenolic and flavonoid content in berries (Vaccinium myrtillus L., Vaccinium vitis-idaea L., and Vaccinium corymbosum L.) in your region. The submitted interdisciplinary topic is relevant to everyday life, and it aroused interest and enthusiasm among students. The students had the opportunity to test their theoretical knowledge in practical experimental research, and they attempted to work as researchers.

scholarly journals Antioxidant Capacity of Dark Red Corn � Biochemical Properties Coupled with Electrochemical Evaluation

2020 ◽  
Vol 71 (6) ◽  
pp. 31-41
Author(s):  
Maja Stevanovic ◽  
Sanja Stevanovic ◽  
Marija Mihailovic ◽  
Biljana Kiprovski ◽  
Goran Bekavac ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Biochemical Properties ◽  
Total Phenolic ◽  
Electrochemical Approach ◽  
High Antioxidant Activity ◽  
Corrosion Inhibition Efficiency ◽  
Cv Measurements ◽  
Phenolic And Flavonoid

Biochemistry coupled with electrochemical approach is employed in a fast, relatively simple, yet highly precise detection of a plant extract antioxidant properties. Antioxidant capacity of dark red corn grains was investigated electrochemically using cyclic voltammetry (CV) on glassy carbon electrode (GC). The linear polarization resistance (LPR) measurements were performed for examining the corrosion inhibitive behavior of polyphenolics on mild steel. The consistent positive correlation (r=0.99) was established between total phenolic and flavonoid contents obtained by CV measurements and spectrophotometric antioxidant assay (DPPH test). Both analyses confirm the high antioxidant activity of tested pigments. Determination of the corrosion inhibition efficiency revealed that the red corn pigments have anti-corrosion effect on mild steels.

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