scholarly journals Antioxidant activity, total phenolic and flavonoid content determination of some selected medicinal plants from Kavre District of Nepal

2020 ◽  
Vol 38 ◽  
pp. 39-47
Author(s):  
Rupak Kharel ◽  
Khaga Raj Sharma
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Content Determination ◽  
Phenolic And Flavonoid

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scholarly journals Evaluation of some traditional medicinal plants: phytochemical profile, antibacterial and antioxidant potentials

2021 ◽  
Vol 26 (2) ◽  
pp. 2499-2510
Author(s):  
ARZU UCAR TURKER ◽  
◽  
ARZU BIRINCI YILDIRIM ◽  
ISA TAS ◽  
ERVA OZKAN ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Traditional Knowledge ◽  
Rosmarinic Acid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Array Detector ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

Bioassays provide an authentication of the traditional knowledge of medicinal plants. Antibacterial, antioxidant and phenolic content of nine medicinal plants (Achillea millefolium, Prunella vulgaris, P. laciniata, Lythrum salicaria, Epilobium angustifolium, E. hirsutum, Pedicularis comosa, Agrimonia eupatoria and Verbena officinalis) were investigated in connection with folkloric usages. Aerial parts of nine plant species were extracted with water and methanol (MeOH). Disc diffusion method was performed to evaluate the antibacterial activity of the extracts against ten pathogenic bacteria. Antioxidant activity was determined by using 2,2-diphenyl-1- picrylhydrazil (DPPH) radical photometric assay. Total phenolic and flavonoid content were investigated by using Folin-Ciocaltaeu and aluminum chloride (AlCl3) colorimetric method, respectively. Quantitative analysis of phenolic constituents of nine plants species were performed by high performance liquid chromatography-diode array detector (HPLC-DAD) via chosen ten phenolic standards (gallic acid monohydrate, caffeic acid, rutin hydrate, luteolin-7-O-β-D glucoside, kaempferol, rosmarinic acid, myricetin, quercetin, coumarin and apigenin). E. hirsutum showed the best antibacterial activity against gram-positive bacteria (S auerus, S. epidermidis and S. pyogenes). L. salicaria also demonstrated strong and broad spectrum antibacterial activity. P. laciniata, L. salicaria and E. angustifolium showed potent antioxidant activity. The highest phenolic and flavonoid content was observed with E. hirsutum and A. millefolium, respectively. L. salicaria also had very high amount of total phenol and flavonoid content. HPLC-DAD analysis displayed that P. laciniata and A. eupatoria were the best sources of rosmarinic acid. Furthermore, V. officinalis and P. vulgaris also had remarkable amount of rutin. This study revealed the scientific rationale behind the traditional knowledge of the tested plants. Consistent with traditional usages, the most prominent plants were L. salicaria, E. hirsutum and E. angustifolium in regard to strong antibacterial and antioxidant potentials. These plants may be proper natural sources with potential applications in pharmaceutical and food industry.

scholarly journals Screening of Bio-active Pigments, Antioxidant Activity, Total Phenolic and Flavonoid Content of Some Economically Important Medicinal Plants for Ethno-botanical Uses

2021 ◽  
Vol 9 (10) ◽  
pp. 1767-1774
Author(s):  
Md. Abdul Hakim ◽  
Mohammed Arif Sadik Polash ◽  
Md. Ashrafuzzaman ◽  
Md. Solaiman Ali Fakir
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Chlorophyll A ◽  
Ricinus Communis ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Chlorophyll B ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

An experiment was conducted to determinate the phytochemical properties (chlorophyll-a, chlorophyll-b, carotenoids, antioxidant activity, total phenolic and flavonoid content) in ten medicinal plants namely Syal Kata (Argemone mexicana L.), Akanda (Calotropis gigantea (L.) Dryand.), Dumur (Ficus carica L.), Chalmugra (Gynocardia odorata R.Br.), Kata kachu (Lasia spinosa (L.) Thwaites), Shetodrone (Leucas aspera (Wild.)), Khona (Oroxylum indicum (L.) Kurz), Reri (Ricinus communis L.), Ghat kachu (Typhonium trilobatum (L.) Schott) and Bazna (Zanthoxylum rhetsa DC.) for ethno-botanical uses. The young fresh leaves were harvested and were subjected to methanolic (95%) extract. Total phenolic contents were analysed by using Folin - Ciocalteau method where gallic acid was used as standard. Total phenolic content varied from 94.53 mg GAE/100 g FW (C. gigantea) to 484.88 mg GAE/100 g FW (L. aspera). Total flavonoid contents were performed by using Quercetin as standard. Total flavonoid content varied from 137.25 mg QUE /100 g FW (C. gigantea) to 334.27 mg QUE/100 g FW (G. odorata). Antioxidant activity of these extracts was performed by using DPPH free radical scavenging assay. Total anti-oxidant capacity varied from IC50 value 35.37 mg/mL (A. mexicana) to 90.47 mg/mL (F. carica) where ascorbic acid is used as standard. Results indicated that among the ten medicinal plants phenolics rich in L. aspera, flavonoids rich in G. odorata, antioxidant activity rich in A. mexicana. Z. rhetsa leaf rich in chlorophyll-a content (311.67 mg/100 g FW), L. aspera leaf rich in chlorophyll-b content (157 mg/100 g FW), Z. rhetsa rich in chlorophyll- (a+b) content as 439 mg/100 g FW, A. mexicana leaf rich in carotenoids content as 96 mg/100 g FW.

scholarly journals Determination of antioxidant activity and phenolic compounds for basic standardization of Turkish propolis

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Aslı Özkök ◽  
Merve Keskin ◽  
Aslı Elif Tanuğur Samancı ◽  
Elif Yorulmaz Önder ◽  
Çiğdem Takma
Keyword(s):  
Antioxidant Capacity ◽  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Total Phenolic ◽  
Scaling Analysis ◽  
Flavonoid Content ◽  
Multidimensional Scaling Analysis ◽  
Flavonoid Quercetin ◽  
Phenolic And Flavonoid

AbstractThis study aimed to determine the standard amount of antioxidant content and compounds of the propolis for the standardization of propolis. For this purpose, the total flavonoids, total phenolic, CUPRAC antioxidant capacity content and the diversity of phenolic and flavonoid components of these propolis samples were found by HPLC determined at the 23 propolis samples which were collected different regions of Turkey. Beside that, the similarities and differences of these 23 provinces to each other according to their antioxidant capacities were investigated by multidimensional scaling analysis. The total flavonoid content in the propolis samples were determined between 21.28 and 152.56 mg CE/g. The total phenolic content in the propolis samples was found between 34.53 mg and 259.4 mg GAE/g. CUPRAC antioxidant capacity of the propolis samples and antioxidant range was found from 95.35 to 710.43 mg TE/g. Also, 4 flavonoid [Quercetin (min.1.12–max.4.14 mg/g), Galangin (min.0.72–max.40.79 mg/g), Apigenin (min.1.07–max.17.35 mg/g), Pinocembrin (min.1.32–max.39.92 mg/g] and 6 phenolic acid [Caffeic acid (min.1.20–max.7.6 mg/g), p-Coumaric acid (min.1.26–max.4.47 mg/g), trans-Ferulic acid (min.1.28–max.4.92 mg/g), Protocatechuic acid (1.78 mg/g), trans-Cinnamic acid (min.1.05–max.3.83 mg/g), Caffeic Acid Phenethyl Ester (CAPE) (min.1.41–max.30.15 mg/g)] components were detected as mg/g, in different ratios in propolis samples collected from different regions. The feature of this study, so far, is to have the maximum number of samples representing the Turkish propolis, and so is thought to help to national and international propolis standard workings.

scholarly journals NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

2017 ◽  
Vol 9 (2) ◽  
pp. 166 ◽  
Author(s):  
Sayed A. El-toumy ◽  
Joslin Y. Salib ◽  
Nabila H. Shafik ◽  
Asmaa S. Abd Elkarim ◽  
Gihan A. Mick
Keyword(s):  
Antioxidant Activity ◽  
Methyl Ester ◽  
Secondary Metabolites ◽  
Gallic Acid ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Dpph Method ◽  
Phenolic And Flavonoid

<p><strong>Objective: </strong>The current study was to deal the isolation and identification of secondary metabolites from <em>Polygonum equisetiforme</em> and evaluation of antioxidant activity of its extract.</p><p><strong>Methods: </strong>The methanol-water extract (7:3) of the air-dried aerial parts of <em>Polygonum equisetiforme</em> was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for <em>in vitro</em> antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.</p><p><strong>Results: </strong>Ten secondary metabolites were isolated from <em>Polygonum equisetiforme</em> in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-<em>O</em>-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-<em>O</em>-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-<em>O-β</em>-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-<em>O-β</em>-D-glucucorinde methyl ester (4), quercetin 3-<em>O-</em>β-D-glucopyranoside (5), quercetin 7-<em>O-β-</em>D-glucopyranoside (6),<em> </em>quercetin(7)<sub>, </sub>myricetin (8), <em>P</em><sub>-</sub>methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of <em>P. equisetiforme</em> extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC<sub>50 </sub>= 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.</p><p><strong>Conclusion: </strong><em>Polygonum equisetiforme</em> is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.</p>

scholarly journals Total phenolic and flavonoid compounds, antioxidant and toxicity profile of extract and fractions of paku atai tuber (Angiopteris ferox Copel)

Food Research ◽  
2019 ◽  
pp. 734-740
Author(s):  
Nur S. ◽  
F. Mubarak ◽  
C. Jannah ◽  
D.A. Winarni ◽  
D.A. Rahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Iron Reduction ◽  
Colorimetric Method ◽  
Total Phenolic ◽  
Toxicity Profile ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Paku atai plant (Angiopteris ferox Copel), specifically the tuber has been used empirically as an anticancer and antidote materials by Dayaknese in West Kutai, with limited scientific study. Thus, this research had been conducted to determine and evaluate the total phenolic, flavonoid content of this plant along with the antioxidant and toxicity profile. The research used several extracts’ solvents including extraction with ethanol (crude extract, CE) and subsequently partitioned with n-hexane (HF); ethyl acetate (EF) and ethanol aqueous (EAF). The resulted crude extract and fractions were then analyzed through colorimetric method to determine the phenolic and flavonoid total; with DPPH and FRAP to observe the antioxidant activity; and using BSLT method to evaluate the toxicity activity. The results showed that the EF fraction provided the strongest antioxidant activity with IC50 value of 13.79 μg/mL and iron reduction with FRAP value of 387.5±6.41 µM/g. However, a high correlation was observed between the antioxidant and the total phenolic content (r2 = 0.970 - 0.974) but little correlation in total flavonoid (r2 = 0.345 - 0.373). Furthermore, the EF (19.56±7.35) showed the highest toxicity activity followed by CE (22.42±2.10), HF (39.52±7.38), and EAF (41.75±5.10). Therefore, the paku atai tuber can be potentially developed as a natural antioxidant and anticancer material.

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