scholarly journals Proteomic analyses on the browning of shade-dried Thompson seedless grape

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Fengjuan Liu ◽  
Wenshu Huang ◽  
Zuoshan Feng ◽  
Yongxia Tao ◽  
Yingying Fan ◽  
...  
Keyword(s):  
Protein Profiling ◽  
Thompson Seedless ◽  
Isomerase Activity ◽  
Data Independent Acquisition ◽  
Parallel Reaction Monitoring ◽  
Seedless Grape ◽  
Phenylpropanoid Biosynthesis ◽  
Tyrosine Metabolism ◽  
Seedless Grapes ◽  
Related Proteins

AbstractChina is one of the main producers in the worldwide raisin market. Most China’s raisins are produced in Xinjiang where the Thompson seedless grape (Vitis vinifera L.cv.Thompson seedless) is the main variety of green raisin. However, the browning of Thompson seedless grape during drying has been well-acknowledged as the primary factor affecting the development of the raisin industry. Data independent acquisition (DIA)-based protein profiling was performed on fresh and shade-dried Thompson seedless grapes. As a result, 5431 proteins were identified, among which the amounts of 739 proteins in fresh grape were found to be significantly different with those in dried grape. The functional annotation based on the Blast2GO showed that the ‘organic substance metabolic process’, ‘regulation of molecular function’, ‘enzyme regulator activity’, and ‘isomerase activity’ related proteins became very active during browning. Further analyses revealed that the browning-related proteins, which with significant different amounts in fresh and in dried grapes, are primarily involved in the phenylpropanoid biosynthesis, tyrosine metabolism, phenylalanine metabolism, oxidative phosphorylation metabolism, plutathione metabolism, peroxisome pathway, and fatty acid degradation. And five random differential proteins were verified with parallel reaction monitoring (PRM). The PRM results were in agreement with the DIA data. The main browning-related proteins of Thompson seedless grape were identified in this study. Their properties were tested, and their roles in the browning mechanism were indicated. This will lay base to a better understanding on the enzymatic browning of Thompson seedless grape, and it will also provide guidance for controlling the quality of Thompson seedless grapes in industry.

scholarly journals Integrated Proteotranscriptomics of Human Myometrium in Labor Landscape Reveals the Increased Molecular Associated With Inflammation Under Hypoxia Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Lina Chen ◽  
Lele Wang ◽  
Yihong Luo ◽  
Qian Huang ◽  
Kaiyuan Ji ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Differentially Expressed ◽  
Human Myometrium ◽  
Rna Seq ◽  
Hypoxia Stress ◽  
Data Independent Acquisition ◽  
Parallel Reaction Monitoring ◽  
Liquid Chromatography Tandem Mass ◽  
Related Proteins ◽  
Physiological And Biochemical

During labor, a variety of coordinated physiological and biochemical events cause the myometrium to transition from a quiescent to contractile state; the molecular mechanisms responsible for this transition, however, remain unclear. To better understand this transition at a molecular level, the global transcriptome and proteome of human myometrial samples in labor and those not in labor were investigated through RNA sequencing (RNA-seq) and quantitative liquid chromatography–tandem mass spectrometry (LC-MS/MS) via data-independent acquisition (DIA) and parallel reaction monitoring (PRM) methods. Furthermore, an integrated proteotranscriptomic analysis was performed to explore biological processes and pathway alterations during labor; this analysis identified 1,626 differentially expressed mRNAs (1,101 upregulated, 525 downregulated) and 135 differentially expressed proteins (97 upregulated, 38 downregulated) in myometrium between nonlabor and in labor groups. The comprehensive results of these analyses showed that the upregulated mRNAs and proteins increased inflammation under hypoxia stress in the myometrium under labor, and related proteins and cytokines were validated by PRM and Luminex assays. Our study confirmed the biological process of inflammation and hypoxia in laboring myometrium at the transcriptome and proteome levels and provided recourse to discover new molecular and biological changes during labor.

scholarly journals Extensive Metabolite Profiling in the Unexploited Organs of Black Tiger for Their Potential Valorization in the Pharmaceutical Industry

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 544
Author(s):  
Jianfei Gao ◽  
Kangning Xiong ◽  
Wei Zhou ◽  
Weijie Li
Keyword(s):  
Acid Metabolism ◽  
Metabolite Profile ◽  
Industrial Applications ◽  
Targeted Metabolomics ◽  
Arginine Biosynthesis ◽  
Nutritional Values ◽  
Phenylpropanoid Biosynthesis ◽  
Tyrosine Metabolism ◽  
Organ Specific ◽  
Widely Targeted Metabolomics

Black tiger (Kadsura coccinea (Lem.)) has been reported to hold enormous pharmaceutical potential. The fruit and rhizome of black tiger are highly exploited in the pharmaceutical and other industries. However, the most important organs from the plant such as the leaf and stem are considered biowastes mainly because a comprehensive metabolite profile has not been reported in these organs. Knowledge of the metabolic landscape of the unexploited black tiger organs could help identify and isolate important compounds with pharmaceutical and nutritional values for a better valorization of the species. In this study, we used a widely targeted metabolomics approach to profile the metabolomes of the K. coccinea leaf (KL) and stem (KS) and compared them with the root (KR). We identified 642, 650 and 619 diverse metabolites in KL, KS and KR, respectively. A total of 555 metabolites were mutually detected among the three organs, indicating that the leaf and stem organs may also hold potential for medicinal, nutritional and industrial applications. Most of the differentially accumulated metabolites between organs were enriched in flavone and flavonol biosynthesis, phenylpropanoid biosynthesis, arginine and proline metabolism, arginine biosynthesis, tyrosine metabolism and 2-oxocarboxylic acid metabolism pathways. In addition, several important organ-specific metabolites were detected in K. coccinea. In conclusion, we provide extensive metabolic information to stimulate black tiger leaf and stem valorization in human healthcare and food.

scholarly journals Preharvest Fungicide, Potassium Sorbate, or Chitosan Use on Quality and Storage Decay of Table Grapes

Plant Disease ◽  
2013 ◽  
Vol 97 (3) ◽  
pp. 307-314 ◽  
Author(s):  
E. Feliziani ◽  
J. L. Smilanick ◽  
D. A. Margosan ◽  
M. F. Mansour ◽  
G. Romanazzi ◽  
...  
Keyword(s):  
Gray Mold ◽  
Potassium Sorbate ◽  
Thompson Seedless ◽  
Berry Skin ◽  
Grape Berries ◽  
Table Grapes ◽  
Berry Quality ◽  
Seedless Grape ◽  
And Storage ◽  
Natural Incidence

Potassium sorbate, a program of four fungicides, or one of three chitosan formulations were applied to clusters of ‘Thompson Seedless’ grape berries at berry set, pre-bunch closure, veraison, and 2 or 3 weeks before harvest. After storage at 2°C for 6 weeks, the natural incidence of postharvest gray mold was reduced by potassium sorbate, the fungicide program, or both together in a tank mixture, in 2009 and 2010. In 2011, the experiment was repeated with three chitosan products (OII-YS, Chito Plant, and Armour-Zen) added. Chitosan or fungicide treatments significantly reduced the natural incidence of postharvest gray mold among grape berries. Berries harvested from vines treated by two of the chitosan treatments or the fungicide program had fewer infections after inoculation with Botrytis cinerea conidia. None harmed berry quality and all increased endochitinase activity. Chitosan decreased berry hydrogen peroxide content. One of the chitosan formulations increased quercetin, myricetin, and resveratrol content of the berry skin. In another experiment, ‘Princess Seedless’ grape treated with one of several fungicides before 4 or 6 weeks of cold storage had less decay than the control. Fenhexamid was markedly superior to the other fungicides for control of both the incidence and spread of gray mold during storage.

scholarly journals PHYTOREGULATORS ON BUD FERTILITY AND CLUSTER QUALITY OF ‘THOMPSON SEEDLESS’ GRAPES GRAFTED ONTO ‘RAMSEY’ ROOTSTOCK

2017 ◽  
Vol 30 (1) ◽  
pp. 97-108 ◽  
Author(s):  
PATRÍCIA FERNANDA INÁCIO VILAR ◽  
◽  
EDER IGNÁCIO DE SOUZA ◽  
LAISE DE SOUSA SANTOS ◽  
EMANUEL ALMEIDA MARTINEZ ◽  
...  
Keyword(s):  
Titratable Acidity ◽  
Soluble Solids ◽  
Basal Region ◽  
Thompson Seedless ◽  
Fresh Material ◽  
Fertility Potential ◽  
Seedless Grapes ◽  
Solids Content ◽  
Cluster Quality

ABSTRACT The objective of this work was to evaluate the influence of Trinexapaque-ethyl (TrixE) and benzyladenine (BA) on the fertility of buds and the quality of bunches of „Thompson Seedless‟ grapes grafted onto „Ramsey‟ rootstock, in the Vale São do Francisco. The experimental design consisted of randomized blocks with 16 treatments (doses and number of applications of the plant regulators TrixE and BA), with four replications, each composed of three plants, with one plant per plot. The variables analyzed were: overall potential bud fertility, potential of the basal, median and apical branches of the plant; fertility of potential buds from the basal region of the basal and median branches; fertility of overall real buds, and of the median and apical branches; diameter of the basal, median and apical branches; Falker chlorophyll index a, b and total; sprouting buds, number of clusters per branch, clusters per plant, branches per plant; berry diameter, length, volume; fresh material mass; soluble solids content (SS), titratable acidity (AT) and SS/AT relationship; diameter, length, mass of fresh material from the clusters; shatter, fresh material mass of the rachis and rachis diameter . It was shown that the TrixE applied in isolation , one, two or three times in doses of 20 mg L -1, or associated with BA in a single application of 120 or 240 mg L-1, increased the rate of real fertility, but did notaffect the length of the clusters or the vegetative vigor of the plants.

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