Kinetics of substrate utilization and bacterial growth of crude oil degraded by Pseudomonas aeruginosa

Abstract We report kinetics of Assam crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2, both isolated from Assam refinery sediments. The isolates exhibited appreciable degrees of hydrophobicity, emulsification index and biosurfactant production. Crude oil degradation efficiency of isolates was assessed in (1) liquid medium amended with 1% v/v crude oil and (2) microcosm sediments (125 mg crude oil/ 10 g sand). In liquid culture, the biodegradation rate (k) and half-life (t1/2) values were found to be 0.0383 day -1 and 18.09 days for P. aeruginosa AKS1, and 0.0204 day -1 and 33.97 days in case of Bacillus sp. AKS2. In microcosm sand sediments, the estimated biodegradation rate (k) and half-life (t 1/2) values were 0.0138 day -1 and 50 days for P. aeruginosa AKS1, and 0.0113 day -1 and 61.34 days in case of Bacillus sp. AKS2. The level of nutrient treatment in microcosm sand sediment was 125 µg N & 62.5 µg P/g sediment in case of P. aeruginosa AKS1 and 375 µg N & 37.5 µg P/g sediment in case of Bacillus sp. AKS2. In microcosms without inorganic nutrients, biodegradation rate (k) and half-life (t1/2) values were found to be 0.0069 day -1 and 100 days for P. aeruginosa AKS1 and for Bacillus sp. AKS2, the respective values were found to be 0.0046 day -1 and 150.68 days. Our data provides important information for predictive hydrocarbon degradation in liquid medium and contaminated sediments.

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Kinetics of nutrient enhanced crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2 isolated from Guwahati refinery, India

Environmental Pollution ◽

10.1016/j.envpol.2016.06.008 ◽

2016 ◽

Vol 216 ◽

pp. 548-558 ◽

Cited By ~ 13

Author(s):

Bobby Chettri ◽

Arghya Mukherjee ◽

James S. Langpoklakpam ◽

Dhrubajyoti Chattopadhyay ◽

Arvind K. Singh

Keyword(s):

Pseudomonas Aeruginosa ◽

Crude Oil ◽

Oil Degradation ◽

Bacillus Sp ◽

Crude Oil Degradation ◽

Kinetics Of

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Efficiency and kinetics of Assam crude oil degradation by Pseudomonas aeruginosa and Bacillus sp.

Archives of Microbiology ◽

10.1007/s00203-021-02567-1 ◽

2021 ◽

Author(s):

Bobby Chettri ◽

Ningombam A. Singha ◽

Arvind Kumar Singh

Keyword(s):

Pseudomonas Aeruginosa ◽

Crude Oil ◽

Oil Degradation ◽

Bacillus Sp ◽

Crude Oil Degradation ◽

Kinetics Of

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The Ex-Situ Bioremediation Kinetics of Raw and Treated Crude Oil Polluted Soil Using Aspergillus Niger and Pseudomonas Aeruginosa

10.20944/preprints201807.0202.v1 ◽

2018 ◽

Cited By ~ 1

Author(s):

Modupe Ojewumi ◽

Valentina Ejemen

Keyword(s):

Pseudomonas Aeruginosa ◽

Aspergillus Niger ◽

Crude Oil ◽

Polluted Soil ◽

Total Petroleum Hydrocarbon ◽

Ex Situ ◽

First Order ◽

Two Systems ◽

Kinetics Of ◽

Better Than

The study was done to investigate the kinetics of first order bioremediation. The effectiveness of remediating soils polluted with raw crude oil and treated crude oil using Aspergillus niger (fungi) and Pseudomonas aeruginosa (bacteria) were investigated. Eight systems of 500g soil sample were polluted with both raw and treated crude oil. Four systems were polluted with 40g treated crude oil while the other remaining four systems were polluted with 40g raw crude oil. Two systems with raw crude and treated crude were left as control (RCC and TCC). Raw crude samples were treated with Aspergillus niger only (RCA) and Pseudomonas aeruginosa (RCP) while treated crude samples were also treated with same (TCA) and (TCP) only. The last two systems were treated with both Pseudomonas aeruginosa and Aspergillus niger (RCAP and TCAP). The first order bioremediation kinetics and biostimulant efficiency for these systems were studied by monitoring Total Petroleum Hydrocarbon (TPH). At the end of the bioremediation period, the results obtained showed that treated crude oil polluted soil generally remediated faster and better than raw crude oil polluted soil. The highest level of bioremediation occurred in systems amended with both Pseudomonas aeruginosa and Aspergillus niger which had about 98% TPH decrease.

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The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.9.2.12 ◽

2019 ◽

Vol 9 (02) ◽

Author(s):

Hussein A Kadhum ◽

Thualfakar H Hasan2

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Bacterial Growth ◽

Broad Spectrum ◽

Inhibitory Activity ◽

Bacterial Pathogens ◽

Inhibitory Ability ◽

Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

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Efficiency and Kinetics of Total Petroleum Hydrocarbons (TPHs) Removal from Crude Oil Polluted Arable Soil using Palm Bunch Ash and Tween 80

Chemistry Africa ◽

10.1007/s42250-020-00219-3 ◽

2021 ◽

Author(s):

Godwin James Udo ◽

Nnanake-Abasi O. Offiong ◽

Alfreda Nwadinigwe ◽

Clement O. Obadimu ◽

Aniedi E. Nyong ◽

...

Keyword(s):

Crude Oil ◽

Petroleum Hydrocarbons ◽

Tween 80 ◽

Arable Soil ◽

Total Petroleum Hydrocarbons ◽

Kinetics Of ◽

Palm Bunch

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Crude oil biodegradation potential of biosurfactant-producing Pseudomonas aeruginosa and Meyerozyma sp.

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2021.126276 ◽

2021 ◽

pp. 126276

Author(s):

Ramla Rehman ◽

Muhammad Ishtiaq Ali ◽

Naeem Ali ◽

Malik Badshah ◽

Mazhar Iqbal ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Crude Oil ◽

Oil Biodegradation

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Natural Product Rottlerin Derivatives Targeting Quorum Sensing

Molecules ◽

10.3390/molecules26123745 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3745

Author(s):

Dittu Suresh ◽

Shekh Sabir ◽

Tsz Tin Yu ◽

Daniel Wenholz ◽

Theerthankar Das ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Growth Inhibition ◽

Natural Product ◽

Bacterial Growth ◽

Inhibitory Activity ◽

Chalcone Derivatives ◽

Synthetic Strategy ◽

The Mannich Reaction ◽

Bacterial Growth Inhibition

Rottlerin is a natural product consisting of chalcone and flavonoid scaffolds, both of which have previously shown quorum sensing (QS) inhibition in various bacteria. Therefore, the unique rottlerin scaffold highlights great potential in inhibiting the QS system of Pseudomonas aeruginosa. Rottlerin analogues were synthesised by modifications at its chalcone- and methylene-bridged acetophenone moieties. The synthesis of analogues was achieved using an established five-step synthetic strategy for chalcone derivatives and utilising the Mannich reaction at C6 of the chromene to construct morpholine analogues. Several pyranochromene chalcone derivatives were also generated using aldol conditions. All the synthetic rottlerin derivatives were screened for QS inhibition and growth inhibition against the related LasR QS system. The pyranochromene chalcone structures displayed high QS inhibitory activity with the most potent compounds, 8b and 8d, achieving QS inhibition of 49.4% and 40.6% and no effect on bacterial growth inhibition at 31 µM, respectively. Both compounds also displayed moderate biofilm inhibitory activity and reduced the production of pyocyanin.

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ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters

Microbiology ◽

10.1099/mic.0.024661-0 ◽

2009 ◽

Vol 155 (4) ◽

pp. 1080-1092 ◽

Cited By ~ 28

Author(s):

A. A. Bartosik ◽

J. Mierzejewska ◽

C. M. Thomas ◽

G. Jagura-Burdzy

Keyword(s):

Pseudomonas Aeruginosa ◽

Fluorescence Microscopy ◽

Bacterial Growth ◽

Complete Loss ◽

Physiological Parameters ◽

Wild Type ◽

Partial Removal ◽

Partner Protein ◽

The One ◽

Mutant Phenotypes

Deletions leading to complete or partial removal of ParB were introduced into the Pseudomonas aeruginosa chromosome. Fluorescence microscopy of fixed cells showed that ParB mutants lacking the C-terminal domain or HTH motif formed multiple, less intense foci scattered irregularly, in contrast to the one to four ParB foci per cell symmetrically distributed in wild-type P. aeruginosa. All parB mutations affected both bacterial growth and swarming and swimming motilities, and increased the production of anucleate cells. Similar effects were observed after inactivation of parA of P. aeruginosa. As complete loss of ParA destabilized its partner ParB it was unclear deficiency of which protein is responsible for the mutant phenotypes. Analysis of four parB mutants showed that complete loss of ParB destabilized ParA whereas three mutants that retained the N-terminal 90 aa of ParB did not. As all four parB mutants demonstrate the same defects it can be concluded that either ParB, or ParA and ParB in combination, plays an important role in nucleoid distribution, growth and motility in P. aeruginosa.

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