scholarly journals DNA damage and repair capacity in lymphocyte of chronic obstructive pulmonary diseases patients during physical exercise with oxygen supplementation

Author(s):  
Andréa Lúcia Gonçalves da Silva ◽  
Thaís Evelyn Karnopp ◽  
Augusto Ferreira Weber ◽  
Cassia da Luz Goulart ◽  
Paloma de Borba Scheneiders ◽  
...  
2016 ◽  
Vol 11 ◽  
Author(s):  
Andréa Lúcia Gonçalves da Silva ◽  
Thaís Evelyn Karnopp ◽  
Augusto Ferreira Weber ◽  
Cassia da Luz Goulart ◽  
Paloma de Borba Scheneiders ◽  
...  

Background: We hypothesized that the use of oxygen supplementation during aerobic exercise induces less DNA damage than exercise alone. The aim of this study is to assess the level of DNA damage induced by physical exercise with and without oxygen supplementation in chronic obstructive pulmonary diseases (COPD) patients. Methods: Peripheral blood was collected before and after aerobic exercise in two conditions: (I) aerobic exercise without oxygen supplementation (AE group) and (II) with oxygen supplementation (AE-O2 group). Lymphocytes were collected to perform the alkaline version of the Comet Assay. To assess the susceptibility to exogenous DNA damage, the lymphocytes were treated with methyl methanesulphonate (MMS) for 1-h or 3-h. After 3-h treatment, the percentage of residual damage was calculated assuming the value of 1-h MMS treatment as 100%. Results: AE group showed lower induced damage (1 h of MMS treatment) and consequently less DNA repair compared to AE-O2 group. AE-O2 group showed an increase in the induced DNA damage (1 h of MMS treatment) and an increased DNA repair capacity. Within the AE-O2 group, in the post-exercise situation the induced DNA damage after 1 h of MMS treatment was higher (p = 0.01) than in the pre-exercise. Conclusion: COPD patients who performed physical exercise associated with oxygen supplementation had a better response to DNA damage induced by MMS and a better DNA repair when compared to the condition of physical exercise without oxygen supplementation.Trial registration: UNISC N374.298. Registered 04 JUN 2013 (retrospectively registered).


AGE ◽  
2015 ◽  
Vol 37 (3) ◽  
Author(s):  
Jorge Pinto Soares ◽  
Amélia M. Silva ◽  
Maria Manuel Oliveira ◽  
Francisco Peixoto ◽  
Isabel Gaivão ◽  
...  

2006 ◽  
Vol 167 (2) ◽  
pp. 131-141 ◽  
Author(s):  
Maria Enrica Fracasso ◽  
Denise Doria ◽  
Paola Franceschetti ◽  
Luigi Perbellini ◽  
Luciano Romeo

2007 ◽  
Vol 48 (9) ◽  
pp. 722-727 ◽  
Author(s):  
Konstantina Kontogianni ◽  
Niki Messini-Nikolaki ◽  
Konstantinos Christou ◽  
Konstantinos Gourgoulianis ◽  
Smaragdi Tsilimigaki ◽  
...  

2021 ◽  
Vol 6 (1) ◽  
pp. 1285-1290
Author(s):  
Nilam Kumari Jha ◽  
Komal Kant Jha

Introduction: Anxiety and Depression is a common comorbidity in chronic obstructive pulmonary disease patients. Impaired lung function is a risk factor for depression In COPD patients, reduced recreational activities and social Isolation is a major risk factor resulting in anxiety and depression.  Objective: The objective of this study is to evaluate the prevalence of Anxiety and Depression among the COPD patients at Pulmonology unit of Nobel Medical College Teaching Hospital.  Methodology: A hospital based cross- sectional research design was used for the study. A total of 185 patients with previously diagnosed COPD. Data were collected by using a face to face interview technique in patients to evaluate anxiety and depression using Nepali version of Hospital Anxiety and Depression Scale questionnaire at Nobel Medical College Teaching Hospital, Biratnagar, Nepal from May to August 2020. Data analysis were done by descriptive and inferential statistics.  Results: A total of 185 patients participated in the study, 157 patients (84.9%) had anxiety, 107 patients (57.8%) had depression and 102 patients (55.1%) had both anxiety and depression. There was statistically significant association of anxiety and depression in COPD patients with age, religion, occupation, smoking status, duration of illness, history of previous hospitalization, number of hospitalization in previous year, type of family, domiciliary oxygen therapy.  Conclusion: The study concluded that anxiety and depression had been most prevalent in COPD patients. Therefore health personnel working in the Pulmonology unit should be aware the early assessment and treatment of anxiety and depression of chronic obstructive pulmonary diseases patients. 


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