Quantitative Redox Proteomics Revealed Molecular Mechanisms of Salt Tolerance in the Roots of Sugar Beet Monomeric Addition line M14

2021 ◽  
Author(s):  
He Liu ◽  
Xiaoxue Du ◽  
Jialin Zhang ◽  
Jinna Li ◽  
Sixue Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Salt Stress ◽  
Sugar Beet ◽  
Redox Regulation ◽  
Molecular Mechanisms ◽  
Growth And Yield ◽  
Transcriptional Level ◽  
Addition Line ◽  
Redox Proteomics ◽  
Redox Proteins

Abstract Background: Salt stress is often associated with excessive production of reactive oxygen species (ROS). Oxidative stress caused by the accumulation of ROS is a major factor that negatively affects crop growth and yield. Root is the primary organ that senses and transmits the salt stress signal to the whole plant. How oxidative stress affect redox sensitive proteins in the roots is not known.Results: In this study, the redox proteome of sugar beet M14 roots under salt stress was investigated. Using iTRAQ reporters, we determined that salt stress caused significant changes in the abundance of many proteins (2305 at 20 min salt stress and 2663 at 10 min salt stress). Using iodoTMT reporters, a total of 95 redox proteins were determined to be responsive to salt stress after normalizing again total protein level changes. Notably, most of the differential redox proteins were involved in metabolism, ROS homeostasis, and stress and defense, while a small number play a role in transport, biosynthesis, signal transduction, transcription and photosynthesis. Transcription levels of 14 genes encoding the identified redox proteins were analyzed using qRT-PCR. All the genes were induced by salt stress at the transcriptional level. Conclusions: Based on the redox proteomics results, we construct a map of the regulatory network of M14 root redox proteins in response to salt stress. This study further refines the molecular mechanism of salt resistance at the level of protein redox regulation.

scholarly journals Cys-SH based quantitative redox proteomics of salt induced response in sugar beet monosomic addition line M14

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Jinna Li ◽  
Kun Wang ◽  
Meichao Ji ◽  
Tingyue Zhang ◽  
Chao Yang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Addition Line ◽  
Double Labeling ◽  
Bisphosphate Carboxylase ◽  
Salt Stress Response ◽  
Redox Proteins ◽  
Ros Homeostasis ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Abstract Background Salt stress is a major abiotic stress that limits plant growth, development and productivity. Studying the molecular mechanisms of salt stress tolerance may help to enhance crop productivity. Sugar beet monosomic addition line M14 exhibits tolerance to salt stress. Results In this work, the changes in the BvM14 proteome and redox proteome induced by salt stress were analyzed using a multiplex iodoTMTRAQ double labeling quantitative proteomics approach. A total of 80 proteins were differentially expressed under salt stress. Interestingly, A total of 48 redoxed peptides were identified for 42 potential redox-regulated proteins showed differential redox change under salt stress. A large proportion of the redox proteins were involved in photosynthesis, ROS homeostasis and other pathways. For example, ribulose bisphosphate carboxylase/oxygenase activase changed in its redox state after salt treatments. In addition, three redox proteins involved in regulation of ROS homeostasis were also changed in redox states. Transcription levels of eighteen differential proteins and redox proteins were profiled. (The proteomics data generated in this study have been submitted to the ProteomeXchange and can be accessed via username: [email protected], password: q9YNM1Pe and proteomeXchange# PXD027550.) Conclusions The results showed involvement of protein redox modifications in BvM14 salt stress response and revealed the short-term salt responsive mechanisms. The knowledge may inform marker-based breeding effort of sugar beet and other crops for stress resilience and high yield.

scholarly journals Cys-SH based Quantitative Redox Proteomics of Salt Induced Response in Sugar Beet Monosomic Addition Line M14

2021 ◽  
Author(s):  
Jinna Li ◽  
Meichao Ji ◽  
Tingyue Zhang ◽  
Chao Yang ◽  
He Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Addition Line ◽  
Double Labeling ◽  
Bisphosphate Carboxylase ◽  
Salt Stress Response ◽  
Redox Proteins ◽  
Ros Homeostasis ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Abstract Background: Salt stress is a major abiotic stress that limits plant growth, development and productivity. Studying the molecular mechanisms of salt stress tolerance may help to enhance crop productivity. Sugar beet monosomic addition line M14 exhibits tolerance to salt stress. Results: In this work, the changes in the BvM14 proteome and redox proteome induced by salt stress were analyzed using a multiplex iodoTMTRAQ double labeling quantitative proteomics approach. A total of 80 proteins were differentially expressed under salt stress. Interestingly, 42 potential redox-regulated proteins showed differential redox change under salt stress. A large proportion of the redox proteins were involved in photosynthesis, ROS homeostasis and other pathways. For example, ribulose bisphosphate carboxylase/oxygenase activase changed in its redox state after salt treatments. In addition, three redox proteins involved in regulation of ROS homeostasis were also changed in redox states. Transcription levels of eighteen differential proteins and redox proteins were profiled. Conclusions: The results showed involvement of protein redox modifications in BvM14 salt stress response and revealed the short-term salt responsive mechanisms. The knowledge may inform marker-based breeding effort of sugar beet and other crops for stress resilience and high yield.

Salt stress induced proteome and transcriptome changes in sugar beet monosomic addition line M14

2012 ◽  
Vol 169 (9) ◽  
pp. 839-850 ◽  
Author(s):  
Le Yang ◽  
Chunquan Ma ◽  
Linlin Wang ◽  
Sixue Chen ◽  
Haiying Li
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Addition Line ◽  
Monosomic Addition ◽  
Monosomic Addition Line

scholarly journals Investigation of an Antioxidative System for Salinity Tolerance in Oenanthe javanica

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 940
Author(s):  
Sunjeet Kumar ◽  
Gaojie Li ◽  
Jingjing Yang ◽  
Xinfang Huang ◽  
Qun Ji ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Stress Responses ◽  
Defense Mechanism ◽  
Crop Improvement ◽  
Antioxidant Defense System ◽  
Growth And Yield ◽  
Oenanthe Javanica ◽  
Water Dropwort

Abiotic stress, such as drought and salinity, severely affect the growth and yield of many plants. Oenanthe javanica (commonly known as water dropwort) is an important vegetable that is grown in the saline-alkali soils of East Asia, where salinity is the limiting environmental factor. To study the defense mechanism of salt stress responses in water dropwort, we studied two water dropwort cultivars, V11E0022 and V11E0135, based on phenotypic and physiological indexes. We found that V11E0022 were tolerant to salt stress, as a result of good antioxidant defense system in the form of osmolyte (proline), antioxidants (polyphenols and flavonoids), and antioxidant enzymes (APX and CAT), which provided novel insights for salt-tolerant mechanisms. Then, a comparative transcriptomic analysis was conducted, and Gene Ontology (GO) analysis revealed that differentially expressed genes (DEGs) involved in the carbohydrate metabolic process could reduce oxidative stress and enhance energy production that can help in adaptation against salt stress. Similarly, lipid metabolic processes can also enhance tolerance against salt stress by reducing the transpiration rate, H2O2, and oxidative stress. Furthermore, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that DEGs involved in hormone signals transduction pathway promoted the activities of antioxidant enzymes and reduced oxidative stress; likewise, arginine and proline metabolism, and flavonoid pathways also stimulated the biosynthesis of proline and flavonoids, respectively, in response to salt stress. Moreover, transcription factors (TFs) were also identified, which play an important role in salt stress tolerance of water dropwort. The finding of this study will be helpful for crop improvement under salt stress.

scholarly journals ROS-Mediated Signalling in Bacteria: Zinc-Containing Cys-X-X-Cys Redox Centres and Iron-Based Oxidative Stress

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Darío Ortiz de Orué Lucana ◽  
Ina Wedderhoff ◽  
Matthew R. Groves
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Posttranslational Modifications ◽  
Structural Changes ◽  
Molecular Mechanisms ◽  
Disulfide Bridge ◽  
Transcriptional Level ◽  
Protein Activity ◽  
Antioxidative Stress ◽  
Detection Mechanisms

Bacteria are permanently in contact with reactive oxygen species (ROS), both over the course of their life cycle as well that present in their environment. These species cause damage to proteins, lipids, and nucleotides, negatively impacting the organism. To detect these ROS molecules and to stimulate the expression of proteins involved in antioxidative stress response, bacteria use a number of different protein-based regulatory and sensory systems. ROS-based stress detection mechanisms induce posttranslational modifications, resulting in overall conformational and structural changes within sensory proteins. The subsequent structural rearrangements result in changes of protein activity, which lead to regulated and appropriate response on the transcriptional level. Many bacterial enzymes and regulatory proteins possess a conserved signature, the zinc-containing redox centre Cys-X-X-Cys in which a disulfide bridge is formed upon oxidative stress. Other metal-dependent oxidative modifications of amino acid side-chains (dityrosines, 2-oxo-histidines, or carbonylation) also modulate the activity of redox-sensitive proteins. Using molecular biology, biochemistry, biophysical, and structure biology tools, molecular mechanisms involved in sensing and response to oxidative stress have been elucidated in detail. In this review, we analyze some examples of bacterial redox-sensing proteins involved in antioxidative stress response and focus further on the currently known molecular mechanism of function.

scholarly journals Salinity stress-induced modification of pectin activates stress signaling pathways and requires HERK/THE and FER to attenuate the response

2020 ◽  
Author(s):  
Nora Gigli-Bisceglia ◽  
Eva Van Zelm ◽  
Wenying Huo ◽  
Jasper Lamers ◽  
Christa Testerink
Keyword(s):  
Cell Wall ◽  
Salt Stress ◽  
Signaling Pathways ◽  
Stress Responses ◽  
Molecular Mechanisms ◽  
Structural Integrity ◽  
Marker Gene ◽  
Mitogen Activated Protein Kinase ◽  
Growth And Yield ◽  
Receptor Like Kinase

AbstractSoil salinity is an increasing worldwide problem for agriculture, affecting plant growth and yield. In our attempt to understand the molecular mechanisms activated in response to salt in plants, we investigated the Catharanthus roseus Receptor like Kinase 1 Like (CrRLK1L) family, which contains well described sensors previously shown to be involved in maintaining and sensing the structural integrity of the cell walls. We have observed that herk1the1-4 double mutants, lacking the function of the Arabidopsis thaliana Receptor like Kinase HERKULES1 combined with a gain of function allele of THESEUS1, phenocopied the phenotypes previously reported in plants lacking FERONIA (FER) function. We report that both fer-4 and herk1the1-4 mutants respond strongly to salt application, resulting in a more intense activation of early and late stress responses. We also show that salt triggers de-methyl esterification of loosely bound pectins. These cell wall modifications might be partly responsible for the activation of the signaling pathways required to activate salt stress responses. In fact, by adding calcium chloride or by chemically inhibiting pectin methyl esterase (PME) activity we observed reduced activation of the early signaling protein Mitogen Activated Protein Kinase 6 (MPK6) as well as a reduced amplitude in salt-induced marker gene induction. We show that MPK6 is required for the full induction of the salt-induced gene expression markers we tested. However, the sodium specific root halotropism response is likely regulated by a different branch of the pathway being independent of MPK6 or calcium application but influenced by the cell wall sensors FER/HERK1/THE1-4 and PME activity. We hypothesize a model where salt-triggered modification of pectin requires the functionality of FER alone or the HERK1/THE1 combination to attenuate salt responses. Collectively, our results show the complexity of salt stress responses and salt sensing mechanisms and their connection to cell wall modifications, likely being in part responsible for the response phenotypes observed in salt treated plants.

scholarly journals Genome-Wide Identification of Na+/H+ Antiporter (NHX) Genes in Sugar Beet (Beta vulgaris L.) and Their Regulated Expression under Salt Stress

Genes ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 401 ◽  
Author(s):  
Guo-Qiang Wu ◽  
Jin-Long Wang ◽  
Shan-Jia Li
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Pcr Analysis ◽  
Functional Identification ◽  
Interacting Protein ◽  
Protein Protein Interaction ◽  
Moderate Salinity

Salinity is one of the major environment factors that limits the growth of plants and the productivity of crops worldwide. It has been shown that Na+ transporters play a central role in salt tolerance and development of plants. The objective of this study was to identify Na+/H+ antiporter (NHX) genes and investigate their expression patterns in sugar beet (Beta vulgaris L.) subjected to various concentrations of NaCl. A total of five putative NHX genes were identified and distributed on four chromosomes in sugar beet. Phylogenetic analysis revealed that these BvNHX genes are grouped into three major classes, viz Vac- (BvNHX1, -2 and -3), Endo- (BvNHX4), and PM-class NHX (BvNHX5/BvSOS1), and within each class the exon/intron structures are conserved. The amiloride-binding site is found in TM3 at N-terminus of Vac-class NHX proteins. Protein-protein interaction (PPI) prediction suggested that only BvNHX5 putatively interacts with calcineurin B-like proteins (CBL) and CBL-interacting protein kinases (CIPK), implying it might be the primary NHX involved in CBL-CIPK pathway under saline condition. It was also found that BvNHX5 contains one abscisic acid (ABA)-responsive element (ABRE), suggesting that BvNHX5 might be involved in ABA signal responsiveness. Additionally, the qRT-PCR analysis showed that all the BvNHX genes in both roots and leaves are significantly up-regulated by salt, and the transcription levels under high salinity are significantly higher than those under either low or moderate salinity. Taken together, this work gives a detailed overview of the BvNHX genes and their expression patterns under salt stress. Our findings also provide useful information for elucidating the molecular mechanisms of Na+ homeostasis and further functional identification of the BvNHX genes in sugar beet.

scholarly journals Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt tolerance

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12683
Author(s):  
Rui Yang ◽  
Zhifen Yang ◽  
Ze Peng ◽  
Fang He ◽  
Luxi Shi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Growth And Yield ◽  
Data Independent Acquisition ◽  
Mechanisms Of Salt Tolerance ◽  
Function Regulator

Background Soil salinity is a major environmental stress that restricts crop growth and yield. Methods Here, crucial proteins and biological pathways were investigated under salt-stress and recovery conditions in Tritipyrum ‘Y1805’ using the data-independent acquisition proteomics techniques to explore its salt-tolerance mechanism. Results In total, 44 and 102 differentially expressed proteins (DEPs) were identified in ‘Y1805’ under salt-stress and recovery conditions, respectively. A proteome-transcriptome-associated analysis revealed that the expression patterns of 13 and 25 DEPs were the same under salt-stress and recovery conditions, respectively. ‘Response to stimulus’, ‘antioxidant activity’, ‘carbohydrate metabolism’, ‘amino acid metabolism’, ‘signal transduction’, ‘transport and catabolism’ and ‘biosynthesis of other secondary metabolites’ were present under both conditions in ‘Y1805’. In addition, ‘energy metabolism’ and ‘lipid metabolism’ were recovery-specific pathways, while ‘antioxidant activity’, and ‘molecular function regulator’ under salt-stress conditions, and ‘virion’ and ‘virion part’ during recovery, were ‘Y1805’-specific compared with the salt-sensitive wheat ‘Chinese Spring’. ‘Y1805’ contained eight specific DEPs related to salt-stress responses. The strong salt tolerance of ‘Y1805’ could be attributed to the strengthened cell walls, reactive oxygen species scavenging, osmoregulation, phytohormone regulation, transient growth arrest, enhanced respiration, transcriptional regulation and error information processing. These data will facilitate an understanding of the molecular mechanisms of salt tolerance and aid in the breeding of salt-tolerant wheat.

Salt stress response of membrane proteome of sugar beet monosomic addition line M14

2015 ◽  
Vol 127 ◽  
pp. 18-33 ◽  
Author(s):  
Haiying Li ◽  
Yu Pan ◽  
Yongxue Zhang ◽  
Chuan Wu ◽  
Chunquan Ma ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Sugar Beet ◽  
Addition Line ◽  
Membrane Proteome ◽  
Salt Stress Response ◽  
Monosomic Addition ◽  
Monosomic Addition Line

scholarly journals Potential Modulation of Sirtuins by Oxidative Stress

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Leonardo Santos ◽  
Carlos Escande ◽  
Ana Denicola
Keyword(s):  
Oxidative Stress ◽  
Posttranslational Modifications ◽  
Histone Deacetylases ◽  
Redox Regulation ◽  
Molecular Mechanisms ◽  
Compensatory Mechanism ◽  
Cysteine Oxidation ◽  
Catalytic Core

Sirtuins are a conserved family of NAD-dependent protein deacylases. Initially proposed as histone deacetylases, it is now known that they act on a variety of proteins including transcription factors and metabolic enzymes, having a key role in the regulation of cellular homeostasis. Seven isoforms are identified in mammals (SIRT1–7), all of them sharing a conserved catalytic core and showing differential subcellular localization and activities. Oxidative stress can affect the activity of sirtuins at different levels: expression, posttranslational modifications, protein-protein interactions, and NAD levels. Mild oxidative stress induces the expression of sirtuins as a compensatory mechanism, while harsh or prolonged oxidant conditions result in dysfunctional modified sirtuins more prone to degradation by the proteasome. Oxidative posttranslational modifications have been identifiedin vitroandin vivo, in particular cysteine oxidation and tyrosine nitration. In addition, oxidative stress can alter the interaction with other proteins, like SIRT1 with its protein inhibitor DBC1 resulting in a net increase of deacetylase activity. In the same way, manipulation of cellular NAD levels by pharmacological inhibition of other NAD-consuming enzymes results in activation of SIRT1 and protection against obesity-related pathologies. Nevertheless, further research is needed to establish the molecular mechanisms of redox regulation of sirtuins to further design adequate pharmacological interventions.

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