scholarly journals Histaminergic interneurons in the ventral nerve cord: assessment of their value for Euarthropod phylogeny

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Maite Maurer ◽  
Janina Hladik ◽  
Thomas M. Iliffe ◽  
Torben Stemme
Keyword(s):  
Nerve Cord ◽  
Laser Scanning ◽  
Ventral Nerve Cord ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Sequence Information ◽  
Histaminergic Neurons ◽  
Character Set ◽  
Phylogenetic Hypotheses ◽  
Structural Aspects

AbstractDespite numerous approaches to the resolution of euarthropod phylogeny, mainly based on modern sequence information and traditional external morphology, the resulting hypotheses are often contradictory and leave many questions about euarthropod evolution unanswered. The comparison of developmental and structural aspects of the nervous system has shown to be a valuable contribution to the assessment of current phylogenetic hypotheses. One promising approach for the generation of new character sets is the morphology of transmitter systems and the discovery of individually identifiable neurons, which allow phylogenetic comparisons on the single cell level. In this context, the serotonin transmitter system has been investigated to a considerable degree. Studies to date have yielded important stimuli to our understanding of euarthropod relationships and the evolution of their nervous systems. However, data on other transmitter systems remain fragmented, and their value with respect to phylogenetic questions remains speculative. The biogenic amine histamine is a promising transmitter; a substantial amount of data has been reported in the literature and the homology of some histaminergic neurons has been suggested. Here, we present a comprehensive review of histaminergic neurons in the ventral nerve cord of Euarthropoda. Using immunocytochemical labeling of histamine combined with confocal laser-scanning microscopy, we investigated the transmitter system in phylogenetically relevant taxa, such as Zygentoma, Remipedia, Diplopoda, and Arachnida. By reconstructing ground patterns, we evaluated the significance of this specific character set for euarthropod phylogeny. With this approach, we identified a set of neurons, which can be considered homologous within the respective major taxon. In conclusion, the histaminergic system contains useful information for our understanding of euarthropod phylogeny, supporting the proposed clades Tetraconata and Mandibulata. Furthermore, this character set has considerable potential to help resolve relationships within the major clades at a deeper level of taxonomy, due to the considerable variability in neurite morphology.

scholarly journals From swimming towards sessility in two metamorphoses – the drastic changes in structure and function of the nervous system of the bay barnacle Amphibalanus improvisus (Crustacea, Thecostraca, Cirripedia) during development

2020 ◽  
Vol 89 (3) ◽  
pp. 324-352
Author(s):  
Paul Kalke ◽  
Thomas Frase ◽  
Stefan Richter
Keyword(s):  
Nervous System ◽  
Peripheral Nervous System ◽  
Nerve Cord ◽  
Laser Scanning ◽  
Ventral Nerve Cord ◽  
Developmental Stages ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Compound Eyes ◽  
Cephalic Shield

Knowledge about the development of the nervous system in cirripeds is limited, particularly with regard to the changes that take place during the two metamorphoses their larvae undergo. This study delivers the first detailed description of the development of the nervous system in a cirriped species, Amphibalanus improvisus by using immunohistochemical labeling against acetylated alpha-tubulin, and confocal laser scanning microscopy. The development of the nervous system in the naupliar stages corresponds largely to that in other crustaceans. As development progresses, the protocerebral sensory organs differentiate and the intersegmental nerves forming the complex peripheral nervous system appear, innervating the sensory structures of the cephalic shield. During metamorphosis into a cypris the lateral sides of the cephalic shield fold down into a bilateral carapace, which leads to a reorganization of the peripheral nervous system. The syncerebrum of the cypris exhibits the highest degree of complexity of all developmental stages, innervating the frontal filaments, nauplius eye, compound eyes and the antennules. During settlement, when the second metamorphosis occur, the closely associated frontal filaments and compound eyes are shed together with the cuticle of the carapace and the antennules. In adults, the syncerebral structures are reduced while the ventral nerve cord and the peripheral nervous system increase in complexity. The peripheral nervous system plays an important role in processing sensory input and also in settlement. In summary, through the larval development we observed a structural and thus also functional increase of complexity in favor of the peripheral nervous system and the ventral nerve cord.

Redescription of P. dorsipapillatus (von Marenzeller, 1893) and additional comments on the other species in the genus Pholoides Pruvot, 1895 (Polychaeta: Sigalionidae)

Zootaxa ◽  
2018 ◽  
Vol 4450 (1) ◽  
pp. 91
Author(s):  
KATHARINA HINTZE ◽  
KARIN MEIßNER ◽  
ANDREAS BICK
Keyword(s):  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Type Material ◽  
Confocal Laser ◽  
Small Scale ◽  
Sequence Information ◽  
Small Species ◽  
Mitochondrial Coi ◽  
Excellent Tool ◽  
Scanning Electron

Pholoides Pruvot, 1895 is a species-poor genus of small scale-bearing polychaetes. Pholoides species are restricted to the continental shelf, living in sandy and muddy substrates, or on hard bottoms. During the DIVA 3 expedition grab samples were taken on four seamounts of the Meteor Seamount complex. One of the most common polychaetes in these samples was P. dorsipapillatus (von Marenzeller, 1893). Based on this material a comparison with the type material is undertaken leading to a re-description of P. dorsipapillatus. Diagnostic characters of all accepted Pholoides species were evaluated using light, scanning electron and confocal laser scanning microscopy. CLSM in particular proved to be an excellent tool for investigating these small species, and especially the type material. Blossom-like sensory buds, found at certain appendages of P. dorsipapillatus, could be an important diagnostic character to distinguish P. dorsipapillatus from other Pholoides species. Sequence information on three different gene fragments, the mitochondrial COI and 16S and the nuclear H3a, were obtained, and could serve for future phylogenetic and phylogeographic studies. 

scholarly journals Ceraphron krogmanni (Hymenoptera: Ceraphronidae), a new species from Lower Saxony with unusual male genitalia

2018 ◽  
Vol 6 ◽  
pp. e24173
Author(s):  
Jonah Ulmer ◽  
Istvan Miko ◽  
Andrew Deans
Keyword(s):  
Male Genitalia ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Lower Saxony ◽  
Anatomical Structures ◽  
Recent Developments ◽  
Character Set ◽  
Field Imaging ◽  
A New Species

Male genitalia phenotypes of Ceraphron (Jurine, 1807) are informative for species delimitation, but due to their minute size, these characters have not been used extensively. Recent developments in visualisation techniques, e.g. confocal laser scanning microscopy and high resolution bright field imaging, allow for more thorough examination of these minute anatomical structures and the development of a robust, male genitalia-based taxonomic system. We also establish a character set, a template, that will facilitate future revisions of these wasps. Ceraphronkrogmanni sp. nov. is described with outsized male genitalia and multiple diagnostic traits that are unique amongst Ceraphron species.

3-D imaging of cells using a confocal laser scanning microscope and digital image processing

1988 ◽  
Vol 46 ◽  
pp. 96-97
Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Biochemical Processes ◽  
Adjacent Structures

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

Use of confocal laser scanning microscopy and a computer model to understand ink cavitation and filamentation

TAPPI Journal ◽  
2010 ◽  
Vol 9 (10) ◽  
pp. 7-15
Author(s):  
HANNA KOIVULA ◽  
DOUGLAS BOUSFIELD ◽  
MARTTI TOIVAKKA
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Print Quality ◽  
Length Scale ◽  
Offset Printing ◽  
Significant Difference ◽  
Printing Speed

In the offset printing process, ink film splitting has an important impact on formation of ink filaments. The filament size and its distribution influence the leveling of ink and hence affect ink setting and the print quality. However, ink filaments are difficult to image due to their short lifetime and fine length scale. Due to this difficulty, limited work has been reported on the parameters that influence filament size and methods to characterize it. We imaged ink filament remains and quantified some of their characteristics by changing printing speed, ink amount, and fountain solution type. Printed samples were prepared using a laboratory printability tester with varying ink levels and operating settings. Rhodamine B dye was incorporated into fountain solutions to aid in the detection of the filaments. The prints were then imaged with a confocal laser scanning microscope (CLSM) and images were further analyzed for their surface topography. Modeling of the pressure pulses in the printing nip was included to better understand the mechanism of filament formation and the origin of filament length scale. Printing speed and ink amount changed the size distribution of the observed filament remains. There was no significant difference between fountain solutions with or without isopropyl alcohol on the observed patterns of the filament remains.

ACTIVATED SLUDGE FLOC CHARACTERIZATION BY CONFOCAL LASER SCANNING MICROSCOPY

2012 ◽  
Vol 11 (3) ◽  
pp. 669-674 ◽  
Author(s):  
Szabolcs Szilveszter ◽  
Botond Raduly ◽  
Szilard Bucs ◽  
Beata Abraham ◽  
Szabolcs Lanyi ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser

Cuticular structures in the copulatory apparatus of Planorbis planorbis (Linnaeus, 1758) (Gastropoda: Pulmonata: Planorbidae)

2009 ◽  
Vol 18 (1) ◽  
pp. 11-16
Author(s):  
E.V. Soldatenko ◽  
A.A. Petrov
Keyword(s):  
Light Microscopy ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Taxonomic Status ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Copulatory Apparatus ◽  
The Family ◽  
Cuticular Structures

The morphology of the copulatory apparatus and associated cuticular structures in Planorbis planorbis was studied by light microscopy, SEM, TEM and confocal laser scanning microscopy. The significance of these cuticular structures for the taxonomic status of the species and for the systematics of the family Planorbidae in general is discussed.

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