scholarly journals Efficacy of extracellular vesicles from dental pulp stem cells for bone regeneration in rat calvarial bone defects

2021 ◽  
Vol 41 (1) ◽  
Author(s):  
Yuka Imanishi ◽  
Masaki Hata ◽  
Ryohei Matsukawa ◽  
Atsushi Aoyagi ◽  
Maiko Omi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Extracellular Vesicles ◽  
Dental Pulp ◽  
Bone Defects ◽  
Dental Pulp Stem Cells ◽  
The Body ◽  
Histological Observation ◽  
Micro Ct ◽  
Calvarial Bone

Abstract Background Extracellular vesicles (EVs) are known to be secreted by various cells. In particular, mesenchymal stem cell (MSC)-derived EVs (MSC-EVs) have tissue repair capacity and anti-inflammatory properties. Dental pulp stem cells (DPSCs), which are MSCs isolated from pulp tissue, are less invasive to the body than other MSCs and can be collected from young individuals. In this study, we investigated the efficacy of EVs secreted by DPSCs (DPSC-EVs) for bone formation. Methods DPSC-EVs were isolated from the cell culture medium of DPSCs. DPSC-EVs were unilaterally injected along with collagen (COL), beta-tricalcium phosphate (β-TCP) or hydroxyapatite (HA) into rat calvarial bone defects. The effects of DPSC-EVs were analyzed by micro-computed tomography (micro-CT) and histological observation. Results Micro-CT showed that administration of DPSC-EVs with the abovementioned scaffolds resulted in bone formation in the periphery of the defects. DPSC-EVs/COL specifically resulted in bone formation in the center of the defects. Histological observation revealed that DPSC-EVs/COL promoted new bone formation. Administration of DPSC-EVs/COL had almost the same effect on the bone defect site as transplantation of DPSCs/COL. Conclusions These results suggest that DPSC-EVs may be effective tools for bone tissue regeneration.

Human Dental Pulp Stem Cells in Rat Mandibular Bone Defects

2019 ◽  
Vol 207 (3-4) ◽  
pp. 138-148 ◽  
Author(s):  
Rubia Teodoro Stuepp ◽  
Priscilla Barros Delben ◽  
Filipe Modolo ◽  
Andrea Gonçalves Trentin ◽  
Ricardo Castilho Garcez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Dental Pulp ◽  
Bone Defects ◽  
Treated Group ◽  
Dental Pulp Stem Cells ◽  
Control Group ◽  
Mandibular Bone ◽  
Significant Difference ◽  
Human Dental Pulp

This study aimed to evaluate the use of human dental pulp stem cells (hDPSCs) in non-critical-sized mandibular bone defects in rats. hDPSCs from permanent teeth were isolated and engrafted in mandibular bone defects in rats for 7, 14, and 28 days; bone defects without cells formed the control group. Samples were evaluated by scanning electron microscopy (SEM), light microscopy (hematoxylin and eosin staining), and the regeneration area was measured by the Image J program. Before surgery procedures, the human dental pulp cells were characterized as dental pulp stem cells: fusiform morphology, plastic-adherent; expression of CD105, CD73, and CD90; lack of expression of CD45 and CD34, and differentiated into osteoblasts, adipocytes, and chondroblasts. The results indicated that within 7 days the control group presented a pronounced bone formation when compared with the treated group (p < 0.05). After 14 days, the treated group showed an increase in bone formation, but with no statistical difference among the groups (p > 0.05). In the final evaluated period there was no difference between the control group and the treated group (p > 0.05). There was a significant difference between 7 and 14 days (p < 0.05) and between 7 and 28 days (p < 0.05) in the treated group. In conclusion, there is no evidence that the use of hDPSCs in the conditions of this study could improve bone formation in non-critical-sized mandibular bone defects.

Three-dimensional bone formation including vascular networks derived from dental pulp stem cells in vitro

Human Cell ◽  
2018 ◽  
Vol 32 (2) ◽  
pp. 114-124
Author(s):  
Miho Watanabe ◽  
Akihiro Ohyama ◽  
Hiroshi Ishikawa ◽  
Akira Tanaka
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Dental Pulp ◽  
Three Dimensional ◽  
Dental Pulp Stem Cells ◽  
Vascular Networks

scholarly journals Correction to: Three-dimensional bone formation including vascular networks derived from dental pulp stem cells in vitro

Human Cell ◽  
2019 ◽  
Vol 32 (3) ◽  
pp. 401-401 ◽  
Author(s):  
Miho Watanabe ◽  
Akihiro Ohyama ◽  
Hiroshi Ishikawa ◽  
Akira Tanaka
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Dental Pulp ◽  
Three Dimensional ◽  
Dental Pulp Stem Cells ◽  
Vascular Networks

scholarly journals Circ-FURIN Expression Enhances Osteoblast Differentiation In Dental Pulp Stem Cells Via SOX11 Signaling Pathway Sponging miR-125

2021 ◽  
Author(s):  
Fang Ji ◽  
Yueting Lin ◽  
Jing Pan ◽  
Zhao Yang ◽  
Qianhui Ren ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Signaling Pathway ◽  
Dental Pulp ◽  
Osteoblast Differentiation ◽  
Dental Pulp Stem Cells ◽  
Heterotopic Bone ◽  
Heterotopic Bone Formation ◽  
Downstream Target ◽  
Report Analysis

Abstract Background: Many studies have found that circRNA plays a part in osteoblast differentiation. However, its mechanism remains unknown. Methods: High-throughput sequencing was used to identifield the different expression of circRNA during osteogenic dental pulp stem cells (DPSCs) differentiation. Luciferase report analysis and RT-qPCR were used to clarify the expression and regulation relationship among circ-FURIN, miR-125 and SOX11. The heterotopic bone formation experiment was further used to confirm the osteoblast differentiation of DPSC with different expression of circ-FURIN, miR-125 and SOX11. Results: Study indicated that circ-FURIN expression remarkably increased during osteoblast differentiation, yet circ-FURIN knockdown suppressed it. Bioinformatics and luciferase results discovered that miR-125 is the downstream target of circ-FURIN. Furthermore, circ-FURIN upregulation decreased miR-125 expression. MiR-125 upregulation restored the promotion effect of circ-FURIN on osteogenic DPSC differentiation. Luciferase report analysis verified that SOX11 is miR-125 downstream target. miR-125 overexpression suppressed osteogenic DPSC differentiation through targeting SOX11. SOX11 overexpression restored miR-125 inhibitory effect on osteogenic DPSC differentiation. In vivo experiments with heterotopic bone model suggested that circ-FURIN overexpression has crucial function to enhance heterotopic bone formation. Conclusions: In summary, circ-FURIN enhances osteoblast DPSC differentiation via the SOX11 signaling pathway by sponging miR-125. These findings suggest a novel therapeutic target for osteoporosis treatment.

AC electrokinetic isolation and detection of extracellular vesicles from dental pulp stem cells: Theoretical simulation incorporating fluid mechanics

2021 ◽  
Author(s):  
Hanieh Hadady ◽  
Fereshteh Karamali ◽  
Fatemeh Ejeian ◽  
Shaghayegh Haghjooy Javanmard ◽  
Laleh Rafiee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Fluid Mechanics ◽  
Extracellular Vesicles ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Theoretical Simulation
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