scholarly journals Cytoprotective effects of sinapic acid on human keratinocytes (HaCaT) against ultraviolet B

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Gyu Ri Kim ◽  
Kye Hwa Lim
Keyword(s):  
Sinapic Acid ◽  
Human Keratinocytes ◽  
Ultraviolet B

scholarly journals UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Eui Jeong Han ◽  
Seo-Young Kim ◽  
Hee-Jin Han ◽  
Hyun-Soo Kim ◽  
Kil-Nam Kim ◽  
...  
Keyword(s):  
Skin Barrier ◽  
Human Keratinocytes ◽  
Underlying Mechanism ◽  
Ultraviolet B ◽  
Sargassum Horneri ◽  
Cellular Damage ◽  
Protective Effects ◽  
Skin Damage ◽  
Antioxidant Mechanism ◽  
Induced Apoptosis

AbstractThe present study aimed to evaluate the protective effect of a methanol extract of Sargassum horneri (SHM), which contains 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (HTT) and apo-9′-fucoxanthinone, against ultraviolet B (UVB)-induced cellular damage in human keratinocytes and its underlying mechanism. SHM significantly improved cell viability of UVB-exposed human keratinocytes by reducing the generation of intracellular reactive oxygen species (ROS). Moreover, SHM inhibited UVB exposure-induced apoptosis by reducing the formation of apoptotic bodies and the populations of the sub-G1 hypodiploid cells and the early apoptotic cells by modulating the expression of the anti- and pro-apoptotic molecules, Bcl-2 and Bax, respectively. Furthermore, SHM inhibited NF-κB p65 activation by inducing the activation of Nrf2/HO-1 signaling. The cytoprotective and antiapoptotic activities of SHM are abolished by the inhibition of HO-1 signaling. In further study, SHM restored the skin dryness and skin barrier disruption in UVB-exposed human keratinocytes. Based to these results, our study suggests that SHM protects the cells against UVB-induced cellular damages through the Nrf2/HO-1/NF-κB p65 signaling pathway and may be potentially useful for the prevention of UVB-induced skin damage.

A novel apoptosis inducer, apoptosis signal regulating kinase (ASK1), is involved in ultraviolet B-induced apoptosis of cultured human keratinocytes

1998 ◽  
Vol 16 ◽  
pp. S69 ◽  
Author(s):  
Koji Sayama ◽  
Hidenori Ichijo ◽  
Kenshi Yamasaki ◽  
Yashushi Hanakawa ◽  
Yuji Shirakata ◽  
...  
Keyword(s):  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Apoptosis Inducer ◽  
Induced Apoptosis

Activation of VEGFR-2 signaling in response to moderate dose of ultraviolet B promotes survival of normal human keratinocytes

2012 ◽  
Vol 44 (1) ◽  
pp. 246-256 ◽  
Author(s):  
Jian-Wei Zhu ◽  
Xian-Jie Wu ◽  
Dan Luo ◽  
Zhong-Fa Lu ◽  
Sui-Qing Cai ◽  
...  
Keyword(s):  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Moderate Dose ◽  
Normal Human Keratinocytes ◽  
Normal Human

scholarly journals Circadian Expression of TIMP3 Is Disrupted by UVB Irradiation and Recovered by Green Tea Extracts

2019 ◽  
Vol 20 (4) ◽  
pp. 862 ◽  
Author(s):  
Sunyoung Park ◽  
Eun-Soo Lee ◽  
Nok-Hyun Park ◽  
Kyeonghwan Hwang ◽  
Eun-Gyung Cho
Keyword(s):  
Clock Gene ◽  
Regulatory Gene ◽  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Rhythmic Expression ◽  
The Core ◽  
Circadian Expression ◽  
Tnf Α ◽  
Factor Α ◽  
Core Clock Gene

The human skin is the outermost physical barrier and has its own circadian machinery that works either cooperatively with the central clock, or autonomously. Circadian rhythms have been observed in many functions related to epidermal homeostasis including hydration and inflammation, and this functional oscillation is disturbed by ultraviolet radiation (UVR), which is a strong environmental cue. Among the genes estimated to show circadian expression in the skin, metalloproteinase inhibitor 3 (TIMP3), has a rhythmic expression in synchronized human keratinocytes similar to that of the core clock gene PER1 and an epidermal circadian regulatory gene, aquaporin 3 (AQP3) but was antiphase to the core clock gene BMAL1. Tumor necrosis factor-α (TNF-α), the regulatory target of TIMP3 via a disintegrin and metalloproteinase domain 17 (ADAM17), was inversely regulated when TIMP3 expression was downregulated by ultraviolet B (UVB) treatment. When synthetic TIMP3 peptides were applied to the cells, the secretion of TNF-α did not increase following the UVB treatment. Similar to TIMP3 peptides, Camellia sinensis leaf-derived extracts showed a distinguishing efficacy in recovering TIMP3 expression, downregulated by UVB treatment. Together, our results suggest that TIMP3 reversely mediates UVR-induced inflammation by being highly expressed during the daytime; therefore, recovering the circadian expression of TIMP3 using synthetic TIMP3 peptides or bioactive natural ingredients could at least in part inhibit the UVR-induced cellular phenomena.

Ultraviolet B-Radiation Dose Influences the Induction of Apoptosis and p53 in Human Keratinocytes

1997 ◽  
Vol 147 (2) ◽  
pp. 148 ◽  
Author(s):  
Jenny Cotton ◽  
Dan F. Spandau
Keyword(s):  
Radiation Dose ◽  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Ultraviolet B Radiation ◽  
Induction Of Apoptosis

Photo-protective effect of americanin B against ultraviolet B-induced damage in cultured human keratinocytes

2014 ◽  
Vol 38 (3) ◽  
pp. 891-900 ◽  
Author(s):  
Jian Zheng ◽  
Mei Jing Piao ◽  
Ki Cheon Kim ◽  
Cheng Wen Yao ◽  
Ji Won Cha ◽  
...  
Keyword(s):  
Protective Effect ◽  
Human Keratinocytes ◽  
Ultraviolet B

scholarly journals Wounding and UVB Light Synergistically Induce the Biosynthesis of Phenolic Compounds and Ascorbic Acid in Red Prickly Pears (Opuntia ficus-indica cv. Rojo Vigor)

2019 ◽  
Vol 20 (21) ◽  
pp. 5327 ◽  
Author(s):  
Ortega-Hernández ◽  
Nair ◽  
Welti-Chanes ◽  
Cisneros-Zevallos ◽  
Jacobo-Velázquez
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Sinapic Acid ◽  
Ultraviolet B ◽  
Uvb Radiation ◽  
Opuntia Ficus Indica ◽  
Wounding Stress ◽  
Pal Activity ◽  
Fruit Samples ◽  
Prickly Pears

The present study evaluated the effects of ultraviolet B (UVB) radiation and wounding stress, applied alone or combined, on the biosynthesis of phenolic compounds and ascorbic acid in the peel and pulp of red prickly pear (Opuntia ficus-indica cv. Rojo Vigor). Whole and wounded-fruit samples were treated with UVB radiation (6.4 W·m−2) for 0 and 15 min, and stored for 24 h at 16 °C. Phytochemical analyses were performed separately in the peel and pulp. The highest phenolic accumulation occurred after storage of the whole tissue treated with UVB, where the main phenolic compounds accumulated in the peel and pulp were quercetin, sinapic acid, kaempferol, rosmarinic acid, and sinapoyl malate, showing increases of 709.8%, 570.2%, 442.8%, 439.9%, and 186.2%, respectively, as compared with the control before storage. Phenylalanine ammonia-lyase (PAL) activity was increased after storage of the whole and wounded tissue treated with UVB light, and this increase in PAL activity was associated to phenolic accumulation. On the other hand, l-galactono-γ-lactone dehydrogenase (GalLDH) activity and ascorbic acid biosynthesis was enhanced due to UVB radiation, and the effect was increased when UVB was applied in the wounded tissue showing 125.1% and 94.1% higher vitamin C content after storage when compared with the control. Respiration rate was increased due to wounding stress, whereas ethylene production was increased by wounding and UVB radiation in prickly pears. Results allowed the generation of a physiological model explaining the UVB and wound-induced accumulation of phenolic compounds and ascorbic acid in prickly pears, where wounding facilitates UVB to access the underlying tissue and enhances an apparent synergistic response.

Cytoprotective effects of 6′-O-galloylpaeoniflorin against ultraviolet B radiation-induced cell damage in human keratinocytes

2014 ◽  
Vol 50 (7) ◽  
pp. 664-674 ◽  
Author(s):  
Cheng Wen Yao ◽  
Mei Jing Piao ◽  
Ki Cheon Kim ◽  
Jian Zheng ◽  
Ji Won Cha ◽  
...  
Keyword(s):  
Cell Damage ◽  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Ultraviolet B Radiation ◽  
Radiation Induced
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