scholarly journals Effect of different cold storage periods of rearing host eggs on the performance of the parasitoid Trichogramma evanescens (Westwood) (Hymenoptera: Trichogrammatidae)

Author(s):  
Amany Siam ◽  
Nawal Zohdy Mohammed Zohdy ◽  
Alia Mohammed Abd ELHafez ◽  
Laila Eid Moursy ◽  
Hanaa Ahmed E. L. Sherif
2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Naglaa F. Abdel-Hameid ◽  
I. R. M. Elzoghby ◽  
A. L. Mehany ◽  
W. A. A. Sayed

AbstractThe performance of parasitism by the egg parasitoid, Trichogramma evanescens Westwood (Hymenoptera: Trichogrammatidae) on eggs of Angoumois grain moth, Sitotroga cerealella Olivier (Lepidoptera: Gelechiidae) was investigated under cold storage and gamma irradiation treatments of the host eggs. Cold storage treatment could improve the parasitoid mass rearing techniques and reduced the costs of biological control programs, while gamma irradiation might be used as a supplementary support at the times of high demand. The suitability of the S. cerealella eggs, stored at – 20 °C for 0.5, 1, or 2 h. as a host for T. evanescens was evaluated. The sensitivity of S. cerealella eggs to gamma irradiation treatments and the acceptability of irradiated eggs for parasitism by T. evanescens females for the parental P and F1 generations were examined. The results revealed that parasitism was drastically reduced more than adult’s emergence and sex-ratio (% of females) after cold storage periods of S. cerealella eggs. Moreover, the parasitism percentages were relatively reduced to (97.1, 96.1, 93.03, and 92.7 %) after irradiating the S. cerealella eggs at 40, 60, 80, and 100 Gy, respectively than the control (97.3% emergence). The percentages of emergence and females’ percent were slightly decreased by gamma irradiation doses, while, equal preferred by the F1 generation of parasitoid that produced from irradiated S. cerealella eggs.


Author(s):  
Mohamed B. Shawer ◽  
Farag A. Sharshir ◽  
El-Kazafy A. Taha ◽  
Elizabeth Z. Shenishen ◽  
Mohamed M. Hassan ◽  
...  

Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.


2010 ◽  
Vol 48 (01) ◽  
Author(s):  
Q Liu ◽  
DP Schultze ◽  
H Bruns ◽  
M Zorn ◽  
M Büchler ◽  
...  
Keyword(s):  

2019 ◽  
Vol 7 (1) ◽  
pp. 12-22
Author(s):  
Ratu Mutia Fajarani ◽  
Yopi Handoyo ◽  
Raden Hengki Rahmanto

Cooling is the best preservation method than others because the food that has been cooled will remain fresh and will not experience a change in taste, color and aroma, besides all the activities that cause decay will stop so that the cooled food will last longer. (Hartanto, 1984). With the proper cooling engine planning, it can help with spatial adjustments, adjustments to loading, estimation of the power to be used, and budget plans. That is what is commonly called the cooling load calculation. Calculation of cooling load needs to be carried out before planning. This is necessary because the magnitude of the pending load is very influential on the selection of the cooling engine so that the freezing point for preserving food can be accurate. Pendiginan burden is influenced by external and internal factors. With the experimental method, it is obtained the results of the external cooling load as the external cooling load is 11.6 kW, the inner cooling load is 138.8 kW and the performance work coefficient (COP) is 2.


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