scholarly journals Recent advances in applications of multimodal ultrasound-guided photoacoustic imaging technology

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Shanshan Wang ◽  
Yunfeng Zhao ◽  
Ye Xu
Keyword(s):  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Imaging Techniques ◽  
Color Doppler ◽  
Power Doppler ◽  
Infrared Region ◽  
Detection Methods ◽  
Imaging Mode ◽  
Nonlinear Imaging ◽  
The Relationship

Abstract Photoacoustic imaging (PAI) is often performed simultaneously with ultrasound imaging and can provide functional and cellular information regarding the tissues in the anatomical markers of the imaging. This paper describes in detail the basic principles of photoacoustic/ultrasound (PA/US) imaging and its application in recent years. It includes near-infrared-region PA, photothermal, photodynamic, and multimode imaging techniques. Particular attention is given to the relationship between PAI and ultrasonic imaging; the latest high-frequency PA/US imaging of small animals, which involves not only B-mode, but also color Doppler mode, power Doppler mode, and nonlinear imaging mode; the ultrasonic model combined with PAI, including the formation of multimodal imaging; the preclinical imaging methods; and the most effective detection methods for clinical research for the future.

scholarly journals Assessment of the Theranostic Potential of Gold Nanostars—A Multimodal Imaging and Photothermal Treatment Study

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2112 ◽  
Author(s):  
Antoine D’Hollander ◽  
Greetje Vande Velde ◽  
Hilde Jans ◽  
Bram Vanspauwen ◽  
Elien Vermeersch ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tumor Imaging ◽  
Photoacoustic Imaging ◽  
Tumor Therapy ◽  
Infrared Region ◽  
Tumor Cell Death ◽  
Gold Nanostars ◽  
Theranostic Agents

Gold nanoparticles offer the possibility to combine both imaging and therapy of otherwise difficult to treat tumors. To validate and further improve their potential, we describe the use of gold nanostars that were functionalized with a polyethyleneglycol-maleimide coating for in vitro and in vivo photoacoustic imaging (PAI), computed tomography (CT), as well as photothermal therapy (PTT) of cancer cells and tumor masses, respectively. Nanostar shaped particles show a high absorption coefficient in the near infrared region and have a hydrodynamic size in biological medium around 100 nm, which allows optimal intra-tumoral retention. Using these nanostars for in vitro labeling of tumor cells, high intracellular nanostar concentrations could be achieved, resulting in high PAI and CT contrast and effective PTT. By injecting the nanostars intratumorally, high contrast could be generated in vivo using PAI and CT, which allowed successful multi-modal tumor imaging. PTT was successfully induced, resulting in tumor cell death and subsequent inhibition of tumor growth. Therefore, gold nanostars are versatile theranostic agents for tumor therapy.

A smart off–on copper sulfide photoacoustic imaging agent based on amorphous–crystalline transition for cancer imaging

2018 ◽  
Vol 54 (78) ◽  
pp. 10962-10965 ◽  
Author(s):  
Qunfeng Feng ◽  
Yanjun Xu ◽  
Bing Hu ◽  
Lu An ◽  
Jiaomin Lin ◽  
...  
Keyword(s):  
Copper Sulfide ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Cancer Imaging ◽  
Low Cost ◽  
Infrared Region ◽  
Imaging Agent ◽  
Agent Based ◽  
Near Infrared Region

By exploiting the amorphous–crystalline transition of CuS, which results in greatly increased absorption throughout the entire near-infrared region, a novel smart off–on CuS photoacoustic imaging agent with good photostability and low cost is developed.

Supercontinuum Laser for Spectroscopic Photoacoustic Imaging of Lipids in the Extended Near-Infrared Region

2018 ◽  
Author(s):  
Manoj Kumar Dasa ◽  
Christos Markos ◽  
Michael Maria ◽  
Ivan B. Gonzalo ◽  
Christian R. Petersen ◽  
...  
Keyword(s):  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Infrared Region ◽  
Near Infrared Region ◽  
Supercontinuum Laser

scholarly journals Functional ultrasound speckle decorrelation-based velocimetry of the brain

2019 ◽  
Author(s):  
Jianbo Tang ◽  
Dmitry D. Postnov ◽  
Kivilcim Kilic ◽  
Sefik Evren Erdener ◽  
Blaire Lee ◽  
...  
Keyword(s):  
Blood Flow ◽  
Flow Velocity ◽  
Blood Flow Velocity ◽  
High Speed ◽  
Imaging Techniques ◽  
Color Doppler ◽  
Power Doppler ◽  
Frequency Noise ◽  
Velocity Changes

AbstractA high-speed, contrast free, quantitative ultrasound velocimetry (vUS) for blood flow velocity imaging throughout the rodent brain is developed based on the normalized first order temporal autocorrelation function of the ultrasound field signal. vUS is able to quantify blood flow velocity in both transverse and axial directions, and is validated with numerical simulation, phantom experiments, and in vivo measurements. The functional imaging ability of vUS is demonstrated by monitoring blood flow velocity changes during whisker stimulation in awake mice. Compared to existing power Doppler and color Doppler-based functional ultrasound imaging techniques, vUS shows quantitative accuracy in estimating both axial and transverse flow speeds and resistance to acoustic attenuation and high frequency noise.

scholarly journals The Voyatzis Mansion in Aegina, Greece: A Historical and Architectural Approach and Physicochemical Documentation of the Wall Painting Decoration

Heritage ◽  
2021 ◽  
Vol 4 (4) ◽  
pp. 3630-3651
Author(s):  
Athena Georgia Alexopoulou ◽  
Zoe Georgiadou ◽  
Panagiotis Ilias ◽  
Agathi Anthoula Kaminari
Keyword(s):  
Near Infrared ◽  
Imaging Techniques ◽  
Historical Context ◽  
Infrared Region ◽  
Physicochemical Analysis ◽  
Wall Painting ◽  
Architectural Form ◽  
Application Techniques ◽  
Discrete Structures ◽  
The Aesthetic

This paper refers to the study of the Voyatzis mansion, in the port of Aegina, Greece. The building complex consists of two discrete structures, which were built at different times (before 1830, 1880 and 1890) and have housed either the Voyatzis family home or its business and work premises. The present research is focused on the documentation of the building, which was never published, and combines the architectural form and elements within its historical context as well as the physicochemical analysis of the painted decoration. The key hypothesis investigated is whether Konstantinos Voyatzis transferred the aesthetic approach and application techniques from Symi to Aegina, when he emigrated from his birthplace. The documentation of the ceiling decoration as well as the physicochemical study of the second floor’s internal wall painting decoration in the main rooms was carried out using in situ modern hyperspectral imaging in specific wavelengths of the visible and near-infrared region, as well as in false color infrared mode. Complementary SWIR imaging, using an InGaAs sensor at the range 900–1700 nm was also applied. The assumption was supported by the findings of the imaging techniques, which showed, apart from the secco method, the use of traditional pigments for the wall paintings, such as cobalt blue, brown, red and yellow earth pigments, chrome oxide green and black, sometimes in admixture with white. The use of guidelines and stencil in the case of the hallway was also recorded.

Label-Free Photoacoustic Cell-Tracking In Real-Time

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Liangzhong Xiang ◽  
Moiz Ahmad ◽  
Xiang Hu ◽  
Zhen Cheng ◽  
Lei Xing
Keyword(s):  
Optical Absorption ◽  
Real Time ◽  
Cell Tracking ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Imaging Techniques ◽  
Melanoma Cells ◽  
Photoacoustic Microscopy ◽  
Label Free ◽  
Tracking Method

AbstractCell-tracking method has an important role in detection of metastatic circulating tumor cells (CTCs) and cell-based therapies. Label-free imaging techniques are desirable for cell-tracking because they enable long time observations without photobleaching in living cells or tissues where labeling is not always possible. Photoacoustic microscopy is a label-free imaging technique that offers rich contrast based on nonfluorescent cellular optical absorption associated with intrinsic chromophores and pigments. We show here that photoacoustic imaging is feasible for detecting very low numbers (x 104) of melanoma cells without labeling because of the strong instinct optical absorption of melanin in near-infrared wavelength. Flowing melanoma cells are imaged with micrometerresolution (40 μm) and penetration depths of centimeters (13 mm) in real-time. Photoacoustic imaging as a new cell-tracking method provides a novel modality for cancer screening and offers insights into the underlying biological process of cancer growth and metastasis and cell therapy.

scholarly journals Fluorescence/photoacoustic imaging-guided nanomaterials for highly efficient cancer theragnostic agent

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vu Hoang Minh Doan ◽  
Van Tu Nguyen ◽  
Sudip Mondal ◽  
Thi Mai Thien Vo ◽  
Cao Duong Ly ◽  
...  
Keyword(s):  
Near Infrared ◽  
High Efficiency ◽  
Imaging System ◽  
Photoacoustic Imaging ◽  
Imaging Techniques ◽  
Non Invasive ◽  
Cell Ablation ◽  
Tumor Tissues

AbstractImaging modalities combined with a multimodal nanocomposite contrast agent hold great potential for significant contributions in the biomedical field. Among modern imaging techniques, photoacoustic (PA) and fluorescence (FL) imaging gained much attention due to their non-invasive feature and the mutually supportive characteristic in terms of spatial resolution, penetration depth, imaging sensitivity, and speed. In this present study, we synthesized IR783 conjugated chitosan–polypyrrole nanocomposites (IR-CS–PPy NCs) as a theragnostic agent used for FL/PA dual-modal imaging. A customized FL and photoacoustic imaging system was constructed to perform required imaging experiments and create high-contrast images. The proposed nanocomposites were confirmed to have great biosafety, essentially a near-infrared (NIR) absorbance property with enhanced photostability. The in vitro photothermal results indicate the high-efficiency MDA-MB-231 breast cancer cell ablation ability of IR-CS–PPy NCs under 808 nm NIR laser irradiation. The in vivo PTT study revealed the complete destruction of the tumor tissues with IR-CS–PPy NCs without further recurrence. The in vitro and in vivo results suggest that the demonstrated nanocomposites, together with the proposed imaging systems could be an effective theragnostic agent for imaging-guided cancer treatment.

Image formation analysis of thick biological specimens using three-dimensional power-spectra of through focus series

1994 ◽  
Vol 52 ◽  
pp. 124-125
Author(s):  
Karen F. Han
Keyword(s):  
Inelastic Scattering ◽  
Imaging Techniques ◽  
Mass Density ◽  
Relative Proportion ◽  
Three Dimensional ◽  
Power Spectra ◽  
Image Formation ◽  
Energy Filtering ◽  
Ewald Sphere ◽  
Nonlinear Imaging

The primary focus in our laboratory is the study of higher order chromatin structure using three dimensional electron microscope tomography. Three dimensional tomography involves the deconstruction of an object by combining multiple projection views of the object at different tilt angles, image intensities are not always accurate representations of the projected object mass density, due to the effects of electron-specimen interactions and microscope lens aberrations. Therefore, an understanding of the mechanism of image formation is important for interpreting the images. The image formation for thick biological specimens has been analyzed by using both energy filtering and Ewald sphere constructions. Surprisingly, there is a significant amount of coherent transfer for our thick specimens. The relative amount of coherent transfer is correlated with the relative proportion of elastically scattered electrons using electron energy loss spectoscopy and imaging techniques.Electron-specimen interactions include single and multiple, elastic and inelastic scattering. Multiple and inelastic scattering events give rise to nonlinear imaging effects which complicates the interpretation of collected images.

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