scholarly journals Green synthesis of silver nanoparticles using Omani pomegranate peel extract and two polyphenolic natural products: characterization and comparison of their antioxidant, antibacterial, and cytotoxic activities

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Pasent Gharib Saad ◽  
Rohan Daniel Castelino ◽  
Vimal Ravi ◽  
Issa Sulaiman Al-Amri ◽  
Shah Alam Khan
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antimicrobial Agents ◽  
Color Change ◽  
Average Particle Size ◽  
Cytotoxic Activities ◽  
Average Particle ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Abstract Background Green synthesis of silver nanoparticles (AgNPs) has gained popularity due to the economical and eco-friendly approach associated with it. The aim of the study was to biosynthesize silver nanoparticles using pomegranate peel extract (PPE), quercetin (Q), and gallic acid (GA) and to evaluate their antioxidant, antimicrobial, and cytotoxic activities. Results A sharp color change from yellow to brown, appearance of a peak in a UV spectrum around 413–425 nm, and four intense peaks in XRD spectra matching with the reference silver crystal confirmed the formation of AgNPs. SEM and TEM analysis revealed particles to be mostly spherical with few aggregates. Average particle size distribution and zeta potential was found to be 43.6 nm and −18.01mV, 31.6 nm and −21.5 mV, and 21.7 nm and −27.9 mV for Q-AgNPs, P-AgNPs, and GA-AgNPs, respectively. P-AgNPs showed excellent in vitro antioxidant activity (84.85–89.20%) and cytotoxic activity (100% mortality). P-AgNPs and GA-AgNPs exhibited good antimicrobial activity against the four bacterial strains comparable to the reference antibiotics but Q-AgNPs failed to inhibit the growth of P. vulgaris. Conclusions Omani pomegranate peel extract seems to be a potential and alternative source for the simple, one pot, eco-friendly green synthesis of extracellular stable AgNPs. The synthesized nanoparticles have the potential to be developed as possible antimicrobial agents and antioxidants. Graphical abstract

scholarly journals Green synthesis, characterization, and antimicrobial activity of silver nanoparticles prepared using Trigonella foenum-graecum L. leaves grown in Saudi Arabia

2021 ◽  
Vol 10 (1) ◽  
pp. 421-429
Author(s):  
Humaira Rizwana ◽  
Mona S. Alwhibi ◽  
Hadeel A. Aldarsone ◽  
Manal Ahmed Awad ◽  
Dina A. Soliman ◽  
...  
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Saudi Arabia ◽  
Green Synthesis ◽  
Antimicrobial Agents ◽  
Average Particle Size ◽  
Antimicrobial Activities ◽  
Average Particle ◽  
Pathogenic Yeast ◽  
Trigonella Foenum Graecum L

Abstract Silver nanoparticles (AgNPs) are widely used for medical applications particularly as antimicrobial agents against multidrug-resistant microbial strains. Some plants stimulate the reduction of Ag ions to AgNPs. In this study, we prepared AgNPs via the green synthesis approach using fenugreek leaves grown in Saudi Arabia. Furthermore, we characterized these AgNPs and evaluated their antimicrobial activities against pathogenic yeast, bacteria, and fungi. The ultraviolet-visible peak at 380 nm confirmed the biosynthesis of NPs. Transmission electron microscopy analyses revealed particle size in the range of 9–57 nm with a spherical shape. Dynamic light scattering results confirm slight aggregation as the average particle size was shown as 68.71 nm and a polydispersity index of 0.083. The energy-dispersive X-ray spectroscopy results showed an intense peak at 3 keV, indicating the presence of elemental AgNPs. The synthesized AgNPs efficiently inhibit the growth of both Gram-positive and Gram-negative bacteria; however, varying degree of inhibition was shown toward fungi. The potent antimicrobial ability of the synthesized NPs can be attributed to their small size and round shape. Among all test organisms, the growth of Candida albicans and Helminthosporium sativum was remarkably affected by AgNPs treatment.

Green Synthesis of Silver Nanoparticles Using Jurinea dolomiaea and Biological Activities

2018 ◽  
Vol 18 (12) ◽  
pp. 8386-8391 ◽  
Author(s):  
Muhammad Riaz ◽  
Muhammad Altaf ◽  
Muhammad Qayyum Khan ◽  
Saima Manzoor ◽  
Muhammad Azhar Shekheli ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Color Change ◽  
Biological Activities ◽  
Average Particle Size ◽  
Inhibition Zone ◽  
Root Extract ◽  
Average Particle ◽  
Face Centered Cubic ◽  
Plant Materials

Green syntheses of nanoparticles using plant materials are of tremendous scope. Here we report advantageous green synthesis for silver nanoparticles (AgNPs) using aqueous-root extract of Jurinea dolomiaea and AgNO3. Color change of solution and UV-Vis absorption at 444 nm indicated the formation of AgNPs. XRD confirmed their face centered cubic structure (fcc) with average particle size of 24.58 nm. SEM analysis showed their spherical, cubic and triangular structures. FT-IR indicated the presence of functional groups of reducing and stabilizing phytochemicals. Methanol-root extract of J. dolomiaea revealed high flavonoid (445 mg RE/g) and phenolic contents (92 mg GAE/g). Methanol-extract showed high antioxidant potency (IC50 = 0.494 μg/mL), rationally due to its high phenolic and flavonoid contents. These AgNPs showed the highest and equal antimicrobial activities against Escherichia coli and Pseudomonas aeruginosa (Inhibition zone 11.0 mm) whereas, methanol-roots extract showed equal and intermediate activities (Inhibition zone 8.0 mm) against both pathogens but aqueous extract showed poor activities (Inhibition zone 6.0 mm) against these both pathogens. AgNPs are playing a major role in the field of nanotechnology and nanomedicine due to their antimicrobial and drug delivery efficacy as well as reasonable tolerance in human biology.

scholarly journals Green Synthesis of Silver Nanoparticles Using Pomegranate and Orange Peel Extracts and Their Antifungal Activity against Alternaria solani, the Causal Agent of Early Blight Disease of Tomato

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2363
Author(s):  
Yasser S. Mostafa ◽  
Saad A. Alamri ◽  
Sulaiman A. Alrumman ◽  
Mohamed Hashem ◽  
Zakaria A. Baka
Keyword(s):  
Silver Nanoparticles ◽  
Orange Peel ◽  
Alternaria Solani ◽  
Pomegranate Peel ◽  
Orange Peels ◽  
Crude Extracts ◽  
Biosynthesized Agnps ◽  
Pomegranate Peel Extract ◽  
Orange Peel Extract ◽  
Peel Extract

This study aimed to synthesize silver nanoparticles (AgNPs) by pomegranate and orange peel extracts using a low concentration of AgNO3 solution to controlearly blight of tomato caused by Alternaria solani. The pathogen was isolated from infected tomato plants growing in different areas of Saudi Arabia. The isolates of this pathogen were morphologically and molecularly identified. Extracts from peels of pomegranate and orange fruits effectively developed a simple, quick, eco-friendly and economical method through a synthesis of AgNPs as antifungal agents against A. solani. Phenolic content in the pomegranate peel extract was greater than orange peel extract. Phenolic compounds showed a variation of both peel extracts as identified and quantified by High-Performance Liquid Chromatography. The phenolic composition displayed variability as the pomegranate peel extract exhibited an exorbitant amount of Quercitrin (23.62 mg/g DW), while orange peel extract recorded a high amount of Chlorogenic acid (5.92 mg/g DW). Biosynthesized AgNPs were characterized using UV- visible spectroscopy which recorded an average wavelength of 437 nm and 450 nm for pomegranate and orange peels, respectively. Fourier-transform infrared spectroscopy exhibited 32x73.24, 2223.71, 2047.29 and 1972.46 cm−1, and 3260.70, 1634.62, 1376.62 and 1243.76 cm−1 for pomegranate and orange peels, respectively. Transmission electron microscopy showed spherical shape of nanoparticles. Zetasizer analysis presented negative charge values; −16.9 and −19.5 mV with average particle sizes 8 and 14 nm fin case of pomegranate and orange peels, respectively. In vitro, antifungal assay was done to estimate the possibility of biosynthesized AgNPs and crude extracts of fruit peels to reduce the mycelial growth of A. solani. AgNPs displayed more fungal mycelial inhibition than crude extracts of two peels and AgNO3. We recommend the use of AgNPs synthesized from fruit peels for controlling fungal plant pathogens and may be applied broadly and safely in place by using the chemical fungicides, which display high toxicity for humans.

scholarly journals Preparation, Characterization and Antibacterial Activity of Pomegranate Peel Extract and Chitosan-Silver Nanoparticles Using Electrochemical Method.

2020 ◽  
Vol 17 ◽  
pp. 222-244
Author(s):  
Afaf Sarhan ◽  
M. I. Abdel Hamid ◽  
Sabrien A. Omar ◽  
R. Hanie
Keyword(s):  
Silver Nanoparticles ◽  
Uv Spectroscopy ◽  
Electrochemical Process ◽  
Gravimetric Analysis ◽  
X Ray Diffraction ◽  
Pomegranate Peel ◽  
Vis Spectroscopy ◽  
Pomegranate Peel Extract ◽  
Average Size ◽  
Peel Extract

The present work involves the development of chitosan-pomegranate peel extract and silver nanoparticles (Cs-PPE-AgNPs) using electrochemical process followed by UV irradiation reduction. Formation of silver nanoparticles characterize by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential thermal analysis (DTA), SEM, Swelling and thermal gravimetric analysis (TGA). The obtain nanoparticles average size was 34.6 nm. UV Spectroscopy show new peak at 405nm indicate the formation of AgNPs particles. Cs-PPE and Cs-PPE-AgNPs also demon started a relativity high antibacterial against Escherichia coli and Staphylococcus aureus bacteria.

scholarly journals GREEN SYNTHESIS NANOPARTIKEL PERAK MENGGUNAKAN EKSTRAK DAUN PUCUK IDAT (Cratoxylum glaucum) SEBAGAI BIOREDUKTOR

2018 ◽  
Vol 1 (2) ◽  
pp. 68
Author(s):  
Verry Andre Fabiani ◽  
Febry Sutanti ◽  
Desti Silvia ◽  
Megawati Ayu Putri
Keyword(s):  
Silver Nanoparticles ◽  
Reaction Time ◽  
Green Synthesis ◽  
Silver Nanoparticle ◽  
Average Particle Size ◽  
Water Extract ◽  
Spherical Shape ◽  
Average Particle ◽  
Sem Image ◽  
Uv Visible

An environment friendly technique for green synthesis of silver nanoparticles has been reported. Silver nanoparticle have been synthesized using water extract of leaves of pucuk idat (Cratoxylum glaucum), commonly known as local Bangka plant. The flavonoid and tannin present in the extract act as reducing agent. AgNO3 0,05 M solution was reducing using the extract at room temperature by stirring. Color changing of the solution was detected at 30 minutes reaction time. The color tends to be darker by the increasing of reaction time. Various techniques used to characterize synthesized nanoparticles are UV-Visible spectrophotometer, x-ray diffraction (XRD) and scanning electron microscopy (SEM). UV-Visible spectrophotometer showed absorbance peak in 405 nm, the XRD shows that silver nanoparticles formed are crystalline. The average particle size of Ag nanoparticles estimated from the Scherrer formula is 35,59 nm, while SEM image shows shape of silver nanoparticle is random and some spherical shape.

scholarly journals Green Synthesis of Silver Nanoparticles Using Terminalia Bellirica Leaves Extract

2021 ◽  
Vol 16 (2) ◽  
Author(s):  
Rashmi Rawat ◽  
M. C. Purohit ◽  
Mahender Singh ◽  
Ankit S. Bartwal
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Ag Nanoparticles ◽  
Metallic Nanoparticles ◽  
Skin Diseases ◽  
Average Particle Size ◽  
Spherical Shape ◽  
Average Particle ◽  
Tem Analysis ◽  
Terminalia Bellirica

Nowadays, for green synthesis, different parts of plant extracts are being used for the synthesis of metallic nanoparticles. Green routes for the synthesis of metallic nanoparticles are most favorable method over conventional methods. In this study, we have synthesized Ag nanoparticles from Terminalia bellirica aqueous leaves extract with AgNO3 solution. Recently, silver nanoparticles are being used for their beneficial effects on human health. It has been reported that it helps in lowering in cholesterol, blood pressure, thirst, pile, skin diseases and treatment of diabetes. Ag nanoparticles were characterized by UV-Vis spectrophotometer, XRD, TEM and FTIR techniques. It was found that Ag+ ion reduced into Ag0 and showed absorption band at 447 nm. X-Ray Diffraction for structural determination confirms the crystalline Ag nanoparticles. TEM analysis confirms the average particle size is less than 30 nm with spherical shape. FTIR spectra confirms the presence of active biomolecules (alcohols, phenols, proteins and nitro compounds) of plant leaves extract which played a key role in formation of Ag nanoparticles.

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