scholarly journals Green Synthesis of Silver Nanoparticles Using Pomegranate and Orange Peel Extracts and Their Antifungal Activity against Alternaria solani, the Causal Agent of Early Blight Disease of Tomato

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2363
Author(s):  
Yasser S. Mostafa ◽  
Saad A. Alamri ◽  
Sulaiman A. Alrumman ◽  
Mohamed Hashem ◽  
Zakaria A. Baka
Keyword(s):  
Silver Nanoparticles ◽  
Orange Peel ◽  
Alternaria Solani ◽  
Pomegranate Peel ◽  
Orange Peels ◽  
Crude Extracts ◽  
Biosynthesized Agnps ◽  
Pomegranate Peel Extract ◽  
Orange Peel Extract ◽  
Peel Extract

This study aimed to synthesize silver nanoparticles (AgNPs) by pomegranate and orange peel extracts using a low concentration of AgNO3 solution to controlearly blight of tomato caused by Alternaria solani. The pathogen was isolated from infected tomato plants growing in different areas of Saudi Arabia. The isolates of this pathogen were morphologically and molecularly identified. Extracts from peels of pomegranate and orange fruits effectively developed a simple, quick, eco-friendly and economical method through a synthesis of AgNPs as antifungal agents against A. solani. Phenolic content in the pomegranate peel extract was greater than orange peel extract. Phenolic compounds showed a variation of both peel extracts as identified and quantified by High-Performance Liquid Chromatography. The phenolic composition displayed variability as the pomegranate peel extract exhibited an exorbitant amount of Quercitrin (23.62 mg/g DW), while orange peel extract recorded a high amount of Chlorogenic acid (5.92 mg/g DW). Biosynthesized AgNPs were characterized using UV- visible spectroscopy which recorded an average wavelength of 437 nm and 450 nm for pomegranate and orange peels, respectively. Fourier-transform infrared spectroscopy exhibited 32x73.24, 2223.71, 2047.29 and 1972.46 cm−1, and 3260.70, 1634.62, 1376.62 and 1243.76 cm−1 for pomegranate and orange peels, respectively. Transmission electron microscopy showed spherical shape of nanoparticles. Zetasizer analysis presented negative charge values; −16.9 and −19.5 mV with average particle sizes 8 and 14 nm fin case of pomegranate and orange peels, respectively. In vitro, antifungal assay was done to estimate the possibility of biosynthesized AgNPs and crude extracts of fruit peels to reduce the mycelial growth of A. solani. AgNPs displayed more fungal mycelial inhibition than crude extracts of two peels and AgNO3. We recommend the use of AgNPs synthesized from fruit peels for controlling fungal plant pathogens and may be applied broadly and safely in place by using the chemical fungicides, which display high toxicity for humans.

Evaluation of the Conditions for the Synthesis of Silver Nanoparticles from Orange Peels and its Antibacterial Effect

2020 ◽  
Vol 14 (3) ◽  
pp. 250-258
Author(s):  
Jose E. Quiroz-Hernández ◽  
Oxana V. Kharissova ◽  
Victor E. Aguirre-Arzola ◽  
Guillermo C.G. Martinez-Avila ◽  
Uziel Castillo-Velazquez
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Plant Extracts ◽  
Antibacterial Effect ◽  
Orange Peel ◽  
Orange Peels ◽  
Agroindustrial Waste ◽  
Vis Spectroscopy ◽  
Orange Peel Extract ◽  
Peel Extract

Aims: To use an agroindustrial waste (orange peels) as a source of polyphenols as a reducing medium for obtaining silver nanoparticles by greener method. Background: Several techniques have been employed for AgNPs synthesis, nevertheless, most of them involve the use of toxic chemicals in the process. The use of fungi, bacteria, and plant extracts as subtracts for green synthesis is an ecofriendly alternative, although hypothetic, route for AgNPs large scale synthesis. In the case of plant extracts, it is believed that polyphenols are the biomolecules responsible for the reduction and stabilization of the Ag+ ions into AgNPs, being a sustainable and ecological option; polyphenols could be obtained from plant waste and agroindustrial subproducts. Objective: To develop an efficient, greener, and low-cost method of AgNPs production using natural products. Methods: The basic principle of silver nanoparticles synthesis is the interaction in a mixture of silver nitrate (source of Ag+ ions) and the orange peel extract (reducing and stabilizing agent) under certain conditions. Five treatments were carried out, evaluating several parameters during AgNPs synthesis such as pH, orange peel extract-silver nitrate ratio, time and conditions of incubation, irradiation of UV light, irradiation of microwave, and temperature. Result: The synthesis of silver nanoparticles from an agroindustrial waste as the orange peel was successfully carried out and checked by visual evaluation, UV-Vis spectroscopy, and EDS analysis. The particle size was estimated between 42.82 nm to 151.75 nm, having a spherical and ovoid morphology. Discussion: Through the analysis of several synthesis conditions, it has become possible to establish a suitable treatment to increase antibacterial yield and evaluate morphology and size traits in order to acquire the best conditions for a future industrial scale synthesis. Conclusion: The orange peel aqueous extract resulted as a great source of polyphenols, allowing the successful synthesis of silver nanoparticles in mild conditions. Thus, obtained AgNPs revealed an increased antibacterial effect and potential against Gram-positive bacteria such as Staphyloccocus aureus.

scholarly journals Silver nanoparticles biogenic synthesized using an orange peel extract and their use as an anti-bacterial agent

2014 ◽  
Vol 9 (3) ◽  
pp. 34-40 ◽  
Author(s):  
A. Awad Manal ◽  
A. Hendi Awatif ◽  
M. O. Ortashi Khalid ◽  
F. A. Elradi Dalia ◽  
E. Eisa Nada ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Orange Peel ◽  
Bacterial Agent ◽  
Orange Peel Extract ◽  
Peel Extract

scholarly journals Preparation, Characterization and Antibacterial Activity of Pomegranate Peel Extract and Chitosan-Silver Nanoparticles Using Electrochemical Method.

2020 ◽  
Vol 17 ◽  
pp. 222-244
Author(s):  
Afaf Sarhan ◽  
M. I. Abdel Hamid ◽  
Sabrien A. Omar ◽  
R. Hanie
Keyword(s):  
Silver Nanoparticles ◽  
Uv Spectroscopy ◽  
Electrochemical Process ◽  
Gravimetric Analysis ◽  
X Ray Diffraction ◽  
Pomegranate Peel ◽  
Vis Spectroscopy ◽  
Pomegranate Peel Extract ◽  
Average Size ◽  
Peel Extract

The present work involves the development of chitosan-pomegranate peel extract and silver nanoparticles (Cs-PPE-AgNPs) using electrochemical process followed by UV irradiation reduction. Formation of silver nanoparticles characterize by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential thermal analysis (DTA), SEM, Swelling and thermal gravimetric analysis (TGA). The obtain nanoparticles average size was 34.6 nm. UV Spectroscopy show new peak at 405nm indicate the formation of AgNPs particles. Cs-PPE and Cs-PPE-AgNPs also demon started a relativity high antibacterial against Escherichia coli and Staphylococcus aureus bacteria.

scholarly journals Green synthesis of silver nanoparticles using Omani pomegranate peel extract and two polyphenolic natural products: characterization and comparison of their antioxidant, antibacterial, and cytotoxic activities

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Pasent Gharib Saad ◽  
Rohan Daniel Castelino ◽  
Vimal Ravi ◽  
Issa Sulaiman Al-Amri ◽  
Shah Alam Khan
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antimicrobial Agents ◽  
Color Change ◽  
Average Particle Size ◽  
Cytotoxic Activities ◽  
Average Particle ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Abstract Background Green synthesis of silver nanoparticles (AgNPs) has gained popularity due to the economical and eco-friendly approach associated with it. The aim of the study was to biosynthesize silver nanoparticles using pomegranate peel extract (PPE), quercetin (Q), and gallic acid (GA) and to evaluate their antioxidant, antimicrobial, and cytotoxic activities. Results A sharp color change from yellow to brown, appearance of a peak in a UV spectrum around 413–425 nm, and four intense peaks in XRD spectra matching with the reference silver crystal confirmed the formation of AgNPs. SEM and TEM analysis revealed particles to be mostly spherical with few aggregates. Average particle size distribution and zeta potential was found to be 43.6 nm and −18.01mV, 31.6 nm and −21.5 mV, and 21.7 nm and −27.9 mV for Q-AgNPs, P-AgNPs, and GA-AgNPs, respectively. P-AgNPs showed excellent in vitro antioxidant activity (84.85–89.20%) and cytotoxic activity (100% mortality). P-AgNPs and GA-AgNPs exhibited good antimicrobial activity against the four bacterial strains comparable to the reference antibiotics but Q-AgNPs failed to inhibit the growth of P. vulgaris. Conclusions Omani pomegranate peel extract seems to be a potential and alternative source for the simple, one pot, eco-friendly green synthesis of extracellular stable AgNPs. The synthesized nanoparticles have the potential to be developed as possible antimicrobial agents and antioxidants. Graphical abstract

Microwave-Assisted Green Synthesis of Silver Nanoparticles Using Orange Peel Extract

2013 ◽  
Vol 2 (3) ◽  
pp. 367-376 ◽  
Author(s):  
Genevieve A. Kahrilas ◽  
Laura M. Wally ◽  
Sarah J. Fredrick ◽  
Michael Hiskey ◽  
Amy L. Prieto ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Orange Peel ◽  
Microwave Assisted ◽  
Orange Peel Extract ◽  
Peel Extract

scholarly journals Green and Chemical Silver Nanoparticles and Pomegranate Formulations to Heal Infected Wounds in Diabetic Rats

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1343
Author(s):  
Renan Aparecido Fernandes Scappaticci ◽  
Andresa Aparecida Berretta ◽  
Elina Cassia Torres ◽  
Andrei Felipe Moreira Buszinski ◽  
Gabriela Lopes Fernandes ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Inflammatory Infiltrate ◽  
Diabetic Rats ◽  
Pomegranate Peel ◽  
Considerable Potential ◽  
Infected Wounds ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Infected cutaneous ulcers from diabetic rats with Candida albicans and Streptococcus aureus were treated with spray formulations containing green silver nanoparticles (GS), chemical silver nanoparticles (CS), or pomegranate peel extract (PS). After wound development and infection, the treatments were performed twice per day for 14 days. The wound healing was analyzed on days 2, 7, and 14 through the determination of CFUs, inflammatory infiltrate, angiogenesis, fibroplasia, myeloperoxidase, and collagen determination. Expressive improvement in wound healing was noted using both silver nanoparticles for 7 days. All the treatments were superior to controls and promoted significant S. aureus reduction after 14 days. CS presented better anti-inflammatory results, and GS and CS the highest number of fibroblasts. Despite the techniques’ limitations, GS and CS demonstrated considerable potential for managing infected wounds, especially considering no early strategies prior to the drugs, such as the debridement of these wounds, were included.

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