scholarly journals Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns

2002 ◽  
Vol 43 (8) ◽  
pp. 1335-1340 ◽  
Author(s):  
Iuliana Popa ◽  
Cristina Vlad ◽  
Jacques Bodennec ◽  
Jacques Portoukalian
Keyword(s):  
Aqueous Solutions ◽  
Silica Gel ◽  
Salt Concentration ◽  
High Stability ◽  
Solid Phase ◽  
General Procedure ◽  
Optimal Conditions ◽  
Alkaline Conditions ◽  
Styrene Divinylbenzene ◽  
Multiple Cycles

An investigation was made using a styrene-divinylbenzene copolymer as a solid phase sorbent to recover gangliosides from aqueous solutions. A comparison with octadecyl-bonded (C18) silica gel showed that the general procedure used to purify gangliosides on C18 silica gel could be used with the copolymer. The yield of gangliosides depended on various parameters such as the composition of the conditioning solution, the salt concentration of the loading solution, and the amount of applied gangliosides per gram of copolymer. In optimal conditions, the recovery of gangliosides and other lipids present in the upper phases of partition was higher than 95%. Using radiolabeled gangliosides, it was found that gangliosides present in serum-containing medium could also be quantitatively recovered on copolymer, provided the medium was diluted with an equal volume of methanol prior to its application onto the column.The major advantage of the copolymer is its high stability in acidic or alkaline conditions that allows multiple cycles of cleaning and reconditioning of the sorbent without alteration of its chromatographic properties.

Structure and Sorption Properties of Silica Chemically Modified with Copper Malonate

2015 ◽  
Vol 670 ◽  
pp. 252-257
Author(s):  
Evgeniya A. Pakhnutova ◽  
Yury G. Slizhov
Keyword(s):  
Gas Chromatography ◽  
Solid Phase Extraction ◽  
Aqueous Solutions ◽  
Organic Compounds ◽  
Silica Gel ◽  
Solid Phase ◽  
Sorption Properties ◽  
Phase Extraction ◽  
Chemically Modified ◽  
Structure And Sorption Properties

The sorbent with the grafted layers of malonate copper was obtained on the surface of silica gel Silokhrom S-120 by the method of successive assemblies. Structural, sorption and chromatographic properties of the investigated materials were studied by adsorption of porosimetry, gas chromatography, pH-measurement and Hammett’s method. The sorbent was used for solid phase extraction of organic compounds from aqueous solutions.

scholarly journals SORPTION OF ERYTHROSINE ONTO SILICA GEL MODIFIED BY CETYLPYRIDINIUM BROMIDE

2021 ◽  
Vol 26 (3(79)) ◽  
pp. 77-85
Author(s):  
O. M. Guzenko ◽  
O. M. Zhukovetska ◽  
D. M. Mukienko ◽  
V. V. Shopovalenko ◽  
A. N. Chebotarev ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Silica Gel ◽  
Solid Phase ◽  
Ion Pairs ◽  
Sodium Dodecylsulfate ◽  
Test System ◽  
Competitive Sorption ◽  
Cetylpyridinium Bromide ◽  
Modified Silica Gel ◽  
Water Desorption

In the current paper, adsorbent based on silica gel L 40/100 modified with cetylpyridinium bromide was obtained. The presence of cetylpyridinium cations on the silica gel surface was confirmed by the diffuse reflectance infrared fourier transform spectroscopy method. The sorption conditions of erythrosine from dilute aqueous solutions with the proposed sorbent were studied and optimized. It is shown that the use of modified silica gel allows efficient (>95%) extraction of erythrosine from aqueous solutions. Under optimal sorption conditions (pH 7, sorbent dosage 0.1 g and sorption time is 15 min), the sorption capacity of modified erythrosine sorbents was determined. It is shown that, with increasing temperature, a change in the isotherm type from the H‑type to the L‑type is observed. This change can be explained by the aggregation of erythrosin in solution, which is a competitive sorption process. It was shown that adsorption isotherms were well described by the Langmuir equation. Thermodynamic studies have made it possible to establish the spontaneous sorption. The desorption of erythrosine from the surface of silica gel modified with cetylpyridinium bromide was studied. It is shown that when using solutions of sulfuric acid, sodium hydroxide and distilled water, desorption does not occur. It was shown that the most effective eluent is solution of sodium dodecylsulfate in alkaline medium, and desorption of erythrosine occurs due to the destruction of ion pairs of dye anions with cetylpyridinium cations fixed on the surface. The data obtained can then be used to develop a test system for determination of erythrosine via corresponding colorimetric scales or for quantitative solid phase extraction and adsorption-spectroscopic quantification of erythrosine in some real samples.

Silica gel surface-imprinted solid-phase extraction of Zr(IV) from aqueous solutions

2007 ◽  
Vol 162 (1-2) ◽  
pp. 113-119 ◽  
Author(s):  
Xijun Chang ◽  
Xiaoyan Wang ◽  
Na Jiang ◽  
Qun He ◽  
Yunhui Zhai ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Aqueous Solutions ◽  
Silica Gel ◽  
Solid Phase ◽  
Phase Extraction

Extraction of explosive compounds from aqueous solutions by solid phase extraction using β-cyclodextrin functionalized carbon nanofibers as sorbents

2016 ◽  
Vol 4 (2) ◽  
pp. 2450-2457 ◽  
Author(s):  
Nikita Tawanda Tavengwa ◽  
Nomso Hintsho ◽  
Shane Durbach ◽  
Isabel Weiersbye ◽  
Ewa Cukrowska ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Aqueous Solutions ◽  
Carbon Nanofibers ◽  
Solid Phase ◽  
Phase Extraction

scholarly journals Micellar Electrokinetic Chromatographic Study of the Separation of an Aromatase Inhibitor and a Tryciclic Antidepressant in the Breast Cancer Treatment

2008 ◽  
Vol 3 ◽  
pp. ACI.S939 ◽  
Author(s):  
J. Rodríguez Flores ◽  
A.M. Contento Salcedo ◽  
L. Muñoz Fernández
Keyword(s):  
Micellar Electrokinetic Chromatography ◽  
Solid Phase ◽  
Background Electrolyte ◽  
Kinetic Studies ◽  
Breast Cancer Treatment ◽  
Chromatographic Study ◽  
Optimal Conditions ◽  
Sodium Dodecylsulphate ◽  
Experimental Parameters

Micellar electrokinetic chromatography (MEKC) was investigated for the simultaneous determination of letrozole, imipramine and their metabolites in human urine samples over a concentration range of therapeutic interest. Experimental parameters such as pH of the running electrolyte, sodium dodecylsulphate (SDS) concentration, borate concentration, voltage, etc were investigated. Under optimal conditions of 25 mM SDS, 15 mM borate buffer (pH 9.2), 15% 2-propanol, as background electrolyte; 28 kV and 40 °C, as voltage and cartridge temperature, respectively; resolution between the peaks was greater than 1.7. Before the determination, a solid phase extraction (SPE) procedure with a C18 cartridge was optimized. Good linearity, accuracy, precision, robustness and ruggedness were achieved and detection limits of 12.5 ng/mL for letrozole and its metabolite and 37.5 ng/mL, were obtained for imipramine and their metabolites. Real determinations of these analytes in two patient urines were carried out. Sensitivity achieved in this method is sufficient to perform kinetic studies in humans.

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