scholarly journals Vitamin A combined with retinoic acid increases retinol uptake and lung retinyl ester formation in a synergistic manner in neonatal rats

2006 ◽  
Vol 47 (8) ◽  
pp. 1844-1851 ◽  
Author(s):  
A. Catharine Ross ◽  
Namasivayam Ambalavanan ◽  
Reza Zolfaghari ◽  
Nan-qian Li
Keyword(s):  
Retinoic Acid ◽  
Vitamin A ◽  
Neonatal Rats ◽  
Retinyl Ester ◽  
Ester Formation

scholarly journals Inflammation induced by lipopolysaccharide does not prevent the vitamin A and retinoic acid-induced increase in retinyl ester formation in neonatal rat lungs

2012 ◽  
Vol 109 (10) ◽  
pp. 1739-1745 ◽  
Author(s):  
Lili Wu ◽  
A. Catharine Ross
Keyword(s):  
Gene Expression ◽  
Retinoic Acid ◽  
Vitamin A ◽  
Retinol Binding Protein ◽  
Neonatal Rat ◽  
Control Group ◽  
Retinyl Ester ◽  
Protein Receptor ◽  
Ester Formation ◽  
Lecithin:Retinol Acyltransferase

Vitamin A (VA) plays an important role in post-natal lung development and maturation. Previously, we have reported that a supplemental dose of VA combined with 10 % of all-trans-retinoic acid (VARA) synergistically increases retinol uptake and retinyl ester (RE) storage in neonatal rat lung, while up-regulating several retinoid homeostatic genes including lecithin:retinol acyltransferase (LRAT) and the retinol-binding protein receptor, stimulated by retinoic acid 6 (STRA6). However, whether inflammation has an impact on the expression of these genes and thus compromises the ability of VARA to increase lung RE content is not clear. Neonatal rats, 7- to 8-d-old, were treated with VARA either concurrently with lipopolysaccharide (LPS; Expt 1) or 12 h after LPS administration (Expt 2); in both studies, lung tissue was collected 6 h after VARA treatment, when RE formation is maximal. Inflammation was confirmed by increased IL-6 and chemokine (C–C motif) ligand 2 (CCL2) gene expression in lung at 6 h and C-reactive protein in plasma at 18 h. In both studies, LPS-induced inflammation only slightly reduced, but did not prevent the VARA-induced increase in lung RE. Quantitative RT-PCR showed that co-administration of LPS with VARA slightly attenuated the VARA-induced increase of LRAT mRNA, but not of STRA6 or cytochrome P450 26B1, the predominant RA hydroxylase in lung. By 18 h post-LPS, expression had subsided and none of these genes differed from the level in the control group. Overall, the present results suggest that retinoid homeostatic gene expression is reduced modestly, if at all, by acute LPS-induced inflammation and that VARA is still effective in increasing lung RE under conditions of moderate inflammation.

scholarly journals Opposing actions of cellular retinol-binding protein and alcohol dehydrogenase control the balance between retinol storage and degradation

2004 ◽  
Vol 383 (2) ◽  
pp. 295-302 ◽  
Author(s):  
Andrei MOLOTKOV ◽  
Norbert B. GHYSELINCK ◽  
Pierre CHAMBON ◽  
Gregg DUESTER
Keyword(s):  
Retinoic Acid ◽  
Vitamin A ◽  
Alcohol Dehydrogenase ◽  
Retinol Binding Protein ◽  
Dietary Vitamin ◽  
Gene Encoding ◽  
Retinyl Ester ◽  
Ester Formation ◽  
Retinyl Esters ◽  
Cellular Retinol Binding Protein

Vitamin A homoeostasis requires the gene encoding cellular retinol-binding protein-1 (Crbp1) which stimulates conversion of retinol into retinyl esters that serve as a storage form of vitamin A. The gene encoding alcohol dehydrogenase-1 (Adh1) greatly facilitates degradative metabolism of excess retinol into retinoic acid to protect against toxic effects of high dietary vitamin A. Crbp1−/−/Adh1−/− double mutant mice were generated to explore whether the stimulatory effect of CRBP1 on retinyl ester formation is due to limitation of retinol oxidation by ADH1, and whether ADH1 limits retinyl ester formation by opposing CRBP1. Compared with wild-type mice, liver retinyl ester levels were greatly reduced in Crbp1−/− mice, but Adh1−/− mice exhibited a significant increase in liver retinyl esters. Importantly, relatively normal liver retinyl ester levels were restored in Crbp1−/−/Adh1−/− mice. During vitamin A deficiency, the additional loss of Adh1 completely prevented the excessive loss of liver retinyl esters observed in Crbp1−/− mice for the first 5 weeks of deficiency and greatly minimized this loss for up to 13 weeks. Crbp1−/− mice also exhibited increased metabolism of a dose of retinol into retinoic acid, and this increased metabolism was not observed in Crbp1−/−/Adh1−/− mice. Our findings suggest that opposing actions of CRBP1 and ADH1 enable a large fraction of liver retinol to remain esterified due to CRBP1 action, while continuously allowing some retinol to be oxidized to retinoic acid by ADH1 for degradative retinoid turnover under any dietary vitamin A conditions.

scholarly journals Retinol kinetics in unsupplemented and vitamin A-retinoic acid supplemented neonatal rats: a preliminary model

2014 ◽  
Vol 55 (6) ◽  
pp. 1077-1086 ◽  
Author(s):  
Libo Tan ◽  
Amanda E. Wray ◽  
Michael H. Green ◽  
A. Catharine Ross
Keyword(s):  
Retinoic Acid ◽  
Vitamin A ◽  
Neonatal Rats ◽  
Preliminary Model

scholarly journals Compartmental modeling of whole-body vitamin A kinetics in unsupplemented and vitamin A-retinoic acid-supplemented neonatal rats

2014 ◽  
Vol 55 (8) ◽  
pp. 1738-1749 ◽  
Author(s):  
Libo Tan ◽  
Amanda E. Wray ◽  
Michael H. Green ◽  
A. Catharine Ross
Keyword(s):  
Retinoic Acid ◽  
Vitamin A ◽  
Whole Body ◽  
Compartmental Modeling ◽  
Neonatal Rats

scholarly journals Priming with Retinoic Acid, an Active Metabolite of Vitamin A, Increases Vitamin A Uptake in the Small Intestine of Neonatal Rats

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4275
Author(s):  
Yaqi Li ◽  
Cheng-Hsin Wei ◽  
J. Kalina Hodges ◽  
Michael H. Green ◽  
A. Catharine Ross
Keyword(s):  
Retinoic Acid ◽  
Vitamin A ◽  
Oral Dose ◽  
Canola Oil ◽  
Active Metabolite ◽  
Storage Capacity ◽  
Treatment Time ◽  
Neonatal Rats ◽  
Continuous Increase ◽  
Second Peak

Given that combined vitamin A (VA) and retinoic acid (RA) supplementation stimulated the intestinal uptake of plasma retinyl esters in neonatal rats, we administrated an RA dose as a pretreatment before VA supplementation to investigate the distinct effect of RA on intestinal VA kinetics. On postnatal days (P) 2 and 3, half of the pups received an oral dose of RA (RA group), while the remaining received canola oil as the control (CN). On P4, after receiving an oral dose of 3H-labeled VA, pups were euthanized at selected times (n = 4–6/treatment/time) and intestine was collected. In both CN and RA groups, intestinal VA mass increased dramatically after VA supplementation; however, RA-pretreated pups had relatively higher VA levels from 10 h and accumulated 30% more VA over the 30-h study. Labeled VA rapidly peaked in the intestine of CN pups and then declined from 13 h, while a continuous increase was observed in the RA group, with a second peak at 10 h and nearly twice the accumulation of 3H-labeled VA compared to CN. Our findings indicate that RA pretreatment may stimulate the influx of supplemental VA into the intestine, and the increased VA accumulation suggests a potential VA storage capacity in neonatal intestine.

Effects of retinoic acid on the concentrations of radioactive metabolites of retinol in tissues of rats maintained on a retinol-deficient diet

1991 ◽  
Vol 69 (6) ◽  
pp. 826-830 ◽  
Author(s):  
Pangala V. Bhat ◽  
André Lacroix
Keyword(s):  
High Performance Liquid Chromatography ◽  
Retinoic Acid ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Kidney Tissue ◽  
Experimental Period ◽  
Deficient Diet ◽  
Retinyl Ester ◽  
Effect Of Feeding

The effect of feeding retinoic acid for 2 and 6 days on the metabolism of labeled retinol in tissues of rats maintained on a vitamin A deficient diet was studied. The metabolites of retinol were analyzed by high performance liquid chromatography. Feeding retinoic acid for 2 days significantly reduced the blood retinol and retinyl ester levels without affecting the vitamin A content of the liver. In intestine and testis the content of labeled retinoic acid was decreased significantly by dietary retinoic acid. Addition of retinoic acid to the diet for 6 days resulted, in addition to decreased blood retinol and retinyl ester values, in an increase in the retinyl ester values in the liver. The accumulation of retinyl ester in the retinoic acid fed rat liver was accompanied by an absence of labeled retinoic acid. Kidney tissue was found to contain the highest levels of labeled retinoic acid, retinol, and retinyl esters; dietary retinoic acid did not alter the concentrations of these retinoids in the kidney during the experimental period. Since kidney retained more vitamin A when the liver vitamin A was low and also dietary retinoic acid did not affect the concentrations of radioactive retinoic acid in the kidney, it is suggested that the kidney may play a major role in the production of retinoic acid from retinol in the body.Key words: retinol, retinoic acid, vitamin A deficiency, tissue metabolites, rat.

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