Preclinical in vitro and in vivo evaluation of antitumor activity of poly (ADP-ribose) polymerase inhibition and trastuzumab combined therapy in HER2-overexpressing breast cancer.

Andrographolide 1, a diterpene lactone of Andrographis paniculata, displays in vitro and in vivo antitumor activity against breast cancer models and mouse myeloid leukemia (M1) cells. In the present study, we report the semi-synthesis of andrographolide derivatives and their in vitro activity against A549 (ATCC) (NSCL cancer) cell line. Amongst the derivatives tested, compounds 3- 5 displayed maximum activity, with IC50 values of 22-31 μg/mL.

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99mTc Radiolabeled HA/TPGS-Based Curcumin-Loaded Nanoparticle for Breast Cancer Synergistic Theranostics: Design, in vitro and in vivo Evaluation

International Journal of Nanomedicine ◽

10.2147/ijn.s242490 ◽

2020 ◽

Vol Volume 15 ◽

pp. 2987-2998

Author(s):

Chong Huang ◽

Fen Chen ◽

Ling Zhang ◽

Yue Yang ◽

Xinggang Yang ◽

...

Keyword(s):

Breast Cancer ◽

In Vivo Evaluation

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In vitro and in vivo antileishmanial efficacy of a combination therapy of diminazene and artesunate against Leishmania donovani in BALB/c mice

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652011000300003 ◽

2011 ◽

Vol 53 (3) ◽

pp. 129-132 ◽

Cited By ~ 12

Author(s):

Joshua Muli Mutiso ◽

John Chege Macharia ◽

Mustafa Barasa ◽

Evans Taracha ◽

Alain J. Bourdichon ◽

...

Keyword(s):

Leishmania Donovani ◽

Combined Therapy ◽

Parasite Burden ◽

Mice Model ◽

In Vivo Evaluation ◽

Reference Drug ◽

Drug Treatments ◽

Potential Use

The in vitro and in vivo activity of diminazene (Dim), artesunate (Art) and combination of Dim and Art (Dim-Art) against Leishmania donovani was compared to reference drug; amphotericin B. IC50 of Dim-Art was found to be 2.28 ± 0.24 µg/mL while those of Dim and Art were 9.16 ± 0.3 µg/mL and 4.64 ± 0.48 µg/mL respectively. The IC50 for Amphot B was 0.16 ± 0.32 µg/mL against stationary-phase promastigotes. In vivo evaluation in the L. donovani BALB/c mice model indicated that treatments with the combined drug therapy at doses of 12.5 mg/kg for 28 consecutive days significantly (p < 0.001) reduced parasite burden in the spleen as compared to the single drug treatments given at the same dosages. Although parasite burden was slightly lower (p < 0.05) in the Amphot B group than in the Dim-Art treatment group, the present study demonstrates the positive advantage and the potential use of the combined therapy of Dim-Art over the constituent drugs, Dim or Art when used alone. Further evaluation is recommended to determine the most efficacious combination ratio of the two compounds.

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Toxicity Effects of Anthracycline-Converted Herceptin and Azo-Functionalized Fe3O4 Nanoparticles on the Heart of Patients with Breast Cancer Based on Echocardiography

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2021.18658 ◽

2021 ◽

Vol 21 (2) ◽

pp. 878-885

Author(s):

Li Liu ◽

Tingting Shen ◽

Hongfang Liu ◽

Gen Zhang ◽

Yongfu Shao

Keyword(s):

Breast Cancer ◽

Targeted Delivery ◽

Combined Therapy ◽

Drug Carriers ◽

Temperature Sensitive ◽

Breast Cancer Patients ◽

Clinical Value ◽

Low Toxicity

The multifunctional nano-carrier system can simultaneously achieve multiple functions such as diagnostic imaging, targeted delivery of anti-tumor drugs, and combined therapy. Application potential Fe3O4 magnetic nanoparticles have the characteristics of low toxicity, superparamagnetism and good photothermal properties. Therefore, a multifunctional magnetic nanocarrier with both magnetic targeting and photothermal properties can be prepared by surface modification of Fe3O4 o DOX is an anti-tumor drug widely used in clinical treatment, and its severe toxic and side effects greatly limit its application. In this paper, a temperature-sensitive magnetic nanocarrier was first constructed and proved to have good superparamagnetism, photothermal properties, and biocom-patibility Then, Fe3O4-Azo-DOX drug-loaded nanoparticles were constructed by covalently bonding DOX. The prepared Fe3O4-Azo-DOX nanoparticles have high stability, sensitive photothermal response and low toxicity. Finally, Fe3O4-Azo-DOX was applied to the study of combined photother-motherapy and chemotherapy in vitro and in vivo. Based on Fe3O4 nanoparticles, a temperature-sensitive Fe3O4-Azo nanocarrier was constructed and its related properties were characterized. Furthermore, anthracycline nanodrugs were used in chemotherapy of breast cancer patients, and their effects were analyzed according to echocardiography parameter change. The results show that Fe3O4-Azo nanoparticles have a good photothermal heating effect. MCF-7 breast cancer cells were selected as a model to investigate the cytotoxicity of Fe3O4-Azo. The results proved that they have excellent biocompatibility and can be used as drug carriers. A Fe3O4-Azo nanocarrier was used to load DOX to construct a NIR-responsive nano-drug delivery system. By studying the NIR controlled release of Fe3O4-Azo-DOX under different pH conditions, it can be seen that it has NIR-responsive release function and the best release effect at pH 5.7. It was found that LVEF, LVFS, and E/A were significantly lower after chemotherapy than before (P < 0.05), which had a certain clinical value in cardiotoxicity The in vitro antitumor effect of Fe3O4-Azo-DOX was studied, and the results showed that the combined effect of photothermal-chemotherapy was significantly better than the photothermal treatment based on Fe3O4-Azo carrier alone and the chemotherapy based on free DOX alone.

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Novel folate-targeted docetaxel-loaded nanoparticles for tumour targeting: in vitro and in vivo evaluation

RSC Advances ◽

10.1039/c6ra04466b ◽

2016 ◽

Vol 6 (69) ◽

pp. 64306-64314 ◽

Cited By ~ 4

Author(s):

M. H. Han ◽

Z. T. Li ◽

D. D. Bi ◽

Y. F. Guo ◽

H. X. Kuang ◽

...

Keyword(s):

Breast Cancer ◽

Folic Acid ◽

Cancer Therapy ◽

Targeted Delivery ◽

Tumour Targeting ◽

Breast Cancer Therapy ◽

In Vivo Evaluation ◽

Targeted Delivery System

Cholesterol-PEG1000-FA (folic acid) was synthesized as a stabilizer to encapsulate DTX, for the construction of a promising targeted delivery system for breast cancer therapy.

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