Prospective serial sequencing of CTC/cfDNA and NK cell activity in patients undergoing multimodality treatment for triple negative breast cancer.

2018 ◽  
Vol 36 (15_suppl) ◽  
pp. e24108-e24108
Author(s):  
Hanna Irie ◽  
Paul H. Schmidt ◽  
Elisa R. Port ◽  
Yayoi Kinoshita ◽  
Alan Soto ◽  
...  
2021 ◽  
Vol 7 (5) ◽  
pp. 3301-3314
Author(s):  
Jihong Yuan ◽  
Linghong Yuan ◽  
Yi Hu ◽  
Jingjing Gong ◽  
Yang Tong ◽  
...  

Breast cancer (Breast Cancer, BC) is one of the most common malignant tumors in women in the world. It is the first malignant tumor that causes the death of women in developing countries. It seriously threatens the lives and health of women and causes damage to the family, economy and society. Through psychological nursing intervention, it has a positive effect on the perioperative mood and NK cell activity of breast cancer patients. The purpose of this article is to explore the effects of psychological intervention on the perioperative mood and NK cell activity of breast cancer patients. This article is based on the concept and theory of psychological nursing intervention for female breast cancer patients, and establishes personalized intervention measures in conjunction with clinical practice to intervene the emotions of breast cancer patients during the perioperative period. This article analyzes the nature, intensity, and causes of negative psychology of the subject through in-depth understanding of the subject’s psychological emotions, coping ability, personality characteristics, past emotional experience, and social support, and discovers the different emotional characteristics of the patient, and formulates the personality psychological intervention measures to stimulate positive and optimistic attitudes and ease the degree of negative psychology. This article analyzes the psychological characteristics of several breast cancer patients. Take corresponding psychological intervention measures. Cope with a series of bad psychology caused by image damage caused by total mastectomy. The results and data in this article show that the incidence of anxiety and depression in the control group is higher than that of the psychological intervention group at 7 days after the operation, with P values of 0.0059 and 0. 0215.Psychological intervention reduces the incidence of negative emotions and reduces the negativeness of patients. The intensity of emotion has played a good clinical effect.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Katherine J. Carpenter ◽  
Aurore-Cecile Valfort ◽  
Nick Steinauer ◽  
Arindam Chatterjee ◽  
Suomia Abuirqeba ◽  
...  

AbstractTriple-negative breast cancer (TNBC) is a highly aggressive subtype that is untreatable with hormonal or HER2-targeted therapies and is also typically unresponsive to checkpoint-blockade immunotherapy. Within the tumor microenvironment dysregulated immune cell metabolism has emerged as a key mechanism of tumor immune-evasion. We have discovered that the Liver-X-Receptors (LXRα and LXRβ), nuclear receptors known to regulate lipid metabolism and tumor-immune interaction, are highly activated in TNBC tumor associated myeloid cells. We therefore theorized that inhibiting LXR would induce immune-mediated TNBC-tumor clearance. Here we show that pharmacological inhibition of LXR activity induces tumor destruction primarily through stimulation of CD8+ T-cell cytotoxic activity and mitochondrial metabolism. Our results imply that LXR inverse agonists may be a promising new class of TNBC immunotherapies.


2011 ◽  
Vol 47 ◽  
pp. S362 ◽  
Author(s):  
S. Radenkovic ◽  
G. Konjevic ◽  
T. Srdic Rajic ◽  
L. Stamatovic ◽  
M. Milovic ◽  
...  

2021 ◽  
Author(s):  
Mehdi Manoochehri ◽  
Thomas Hielscher ◽  
Nasim Borhani ◽  
Clarissa Gerhäuser ◽  
Olivia Fletcher ◽  
...  

Abstract Background: A shift in the proportions of blood immune cells is a hallmark of cancer development. Here, we investigated whether methylation-derived immune cell type ratios and methylation-derived neutrophil-to-lymphocyte ratios (mdNLRs) are associated with triple-negative breast cancer (TNBC). Methods: Leukocyte subtype-specific un/methylated CpG sites were selected and methylation levels at these sites used as proxies for immune cell type proportions and mdNLR estimation in 231 TNBC cases and 231 age-matched controls. Data were validated using the Houseman deconvolution method. Additionally, the natural killer (NK) cell ratio was measured in a prospective sample set of 146 TNBC cases and 146 age-matched controls. Results: The mdNLRs were higher in TNBC cases compared with controls and associated with TNBC (odds ratio (OR) range (2.66-4.29), all Padj.<1e-04). A higher neutrophil ratio and lower ratios of NK cells, CD4+ T cells, CD8+ T cells, monocytes, and B cells were associated with TNBC. The strongest association was observed with decreased NK cell ratio (OR range (1.28-1.42), all Padj.<1e-04). The NK cell ratio was also significantly lower in pre-diagnostic samples of TNBC cases compared with controls (P=0.019).Conclusion: This immunomethylomic study shows that a shift in the ratios/proportions of leukocyte subtypes is associated with TNBC, with decreased NK cell showing the strongest association. These findings improve our knowledge of the role of the immune system in TNBC and point to the possibility of using NK cell level as a non-invasive molecular marker for TNBC risk assessment, early detection, and prevention.


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