Exploration of the expression and significance of IL-6, STAT3 and PD-L1 in esophageal squamous cell carcinoma.

e16554 Background: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies worldwide and has a very high morbidity and mortality rate. The IL-6/STAT3 axis can simultaneously promote the expansion of immunosuppressive cells.This study detected IL-6, STAT3 and PD-L1 in ESCC and analyzed the potential clinical application value. Methods: Between July 2018 and April 2019, 70 ESCC patients underwent radical thoracic surgery at Affiliated Hospital of North Sichuan Medical College. RT-PCR was used to detect PD-L1, IL-6, and STAT3 mRNA expression in cancer and adjacent normal tissues. Western blot detection of PD-L1, IL-6, and STAT3 was performed in 40 cancer and adjacent normal tissues. The correlation of PD-L1, IL-6 and STAT3 protein expression with tumor invasion depth, tumor size, lymph node metastasis,clinical stage and other clinicopathological characteristics as well as the correlation between the three proteins were analyzed statistically. Results: The mRNA levels of PD-L1, IL-6, and STAT3 in 70 ESCC patients were significantly different between cancerous and adjacent normal tissues (P < 0.001). In 40 ESCC patients, 28 ESCC tissues showed significantly higher PD-L1 expression than adjacent normal tissue (28/12, P < 0.001), 29 ESCC tissues showed significantly higher STAT3 expression (29/11,P = 0.009 ), and 27 ESCC tissues showed significantly higher IL-6 expression (27/13,P = 0.042). There was no correlation between PD-L1 expression and age, sex, tumor site or maximum tumor diameter, but PD-L1 expression showed a positive correlation with T stage (P = 0.009), lymph node metastasis (P = 0.044), and clinical stage (P = 0.033). There was no correlation between STAT3 expression and age, sex, tumor site, maximum tumor diameter, lymph node metastasis or clinical stage, but STAT3 expression showed a positive correlation with T stage (P = 0.025). IL-6 expression was not correlated with age, sex, maximum tumor diameter, lymph node metastasis or clinical stage, but IL-6 expression showed a positive correlation with T stage ( P = 0.003)and tumor site (P = 0.011). In addition, PD-L1 expression showed a positive correlation with STAT3 (P = 0.013) and IL-6 ( P = 0.008), and STAT3 showed a positive correlation with IL-6 ( P = 0.027). Conclusions: PD-L1, IL-6, and STAT3 are highly expressed in ESCC and their protein levels exhibit a positive correlation. The IL-6/STAT3 signaling pathway proteins may be a potential biomarker predicting the efficacy of immunotherapy by affecting the expression of PD-L1.

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Reciprocal regulation of miR-1205 and E2F1 modulates progression of laryngeal squamous cell carcinoma

Cell Death and Disease ◽

10.1038/s41419-019-2154-4 ◽

2019 ◽

Vol 10 (12) ◽

Cited By ~ 2

Author(s):

Pei Li ◽

Xi-Jun Lin ◽

Yang Yang ◽

An-Kui Yang ◽

Jin-Ming Di ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Cell Carcinoma ◽

Squamous Cell ◽

Laryngeal Squamous Cell Carcinoma ◽

Clinical Stage ◽

Reciprocal Regulation ◽

Node Metastasis ◽

T Stage

AbstractThe burgeoning functions of many microRNAs (miRs) have been well study in cancer. However, the level and function of miR-1205 in laryngeal squamous cell cancer remains unknown. In the current research, we validated that miR-1205 was notably downregulated in human laryngeal squamous cell carcinoma (LSCC) samples in comparison with tissues adjacent to LSCC, and correlated with T stage, lymph node metastasis, and clinical stage. Using Kaplan–Meier analysis indicates that high expression of miR-1205 has a favorable prognosis for patients with LSCC. Functional assays show that enforced miR-1205 expression attenuates the migration, growth, and invasion of LSCC cells. And E2F1 is verified to be a target of miR-1205, while E2F1 binds to miR-1205 promoter and transcriptionally inhibits miR-1205 expression. Overexpression of E2F1 reverses the inhibitory impacts of miR-1205 on LSCC cells in part. Importantly, E2F1 is abnormally increased in LSCC tissues, and its protein levels were inversely relevant to miR-1205 expression. High E2F1 protein level is in connection with clinical stage, T stage, lymph node metastasis, and poor prognosis. Consequently, reciprocal regulation of miR-1205 and E2F1 plays a crucial role in the progression of LSCC, suggesting a new miR-1205/E2F1-based clinical application for patients of LSCC.

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Identification of relevant prognostic values of cytokeratin 20 and cytokeratin 7 expressions in lung cancer

Bioscience Reports ◽

10.1042/bsr20171086 ◽

2017 ◽

Vol 37 (6) ◽

Cited By ~ 2

Author(s):

Hai-Tao Luo ◽

Cai-Xia Liang ◽

Rong-Cheng Luo ◽

Wei-Guang Gu

Keyword(s):

Lung Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Poor Prognosis ◽

Clinical Stage ◽

Clinical Parameters ◽

Cytokeratin 20 ◽

Node Metastasis ◽

T Stage ◽

Positive Expression

Lung cancer is one of the most common malignant tumors harmful to human health. Cytokeratin (CK) is highly conserved and differentiated related to the proliferation and differentiation of epithelial cells. The aim of the study was to explore expressions of CK20 and CK7 and corresponding prognostic values in patients with lung cancer. Our study included 258 cases of patients confirmed with lung cancer. Expressions of CK20 and CK7 mRNA and protein were detected using real-time quantitative PCR (qRT-PCR) and Western blot, respectively, followed by the performance of immunohistochemistry staining. Associations of CK20 and CK7 with the clinical parameters and prognosis of lung cancer patients were further analyzed. There were obvious differences regarding the positive expression of CK20 in different T stage, lymph node metastasis, invasion, size, and clinical stage subgroups; besides, significant differences in the positive expression of CK7 were also observed in subgroups of different sex, age, lymph node metastasis, invasion, and differentiation. Furthermore, effects of age, smoking, T stage, lymph node metastasis and invasion, size, and CK7 expressions were significant on the survival of patients (all P<0.05). Multivariate analysis revealed that lymph node metastasis, T stage, and CK7 expression were independent risk factors for poor prognosis of involved patients (all P<0.05), while age, smoking, and invasion had no marked relation to the survival time of patients with lung cancer (all P>0.05). Positive CK20 and CK7 expressions are detected in patients with lung cancer; positive expression of CK7 associated with pathological features of lymph node metastasis and T stage may be independent clinical parameters for poor prognosis of patients with lung cancer.

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Predictive factors of pathological lateral pelvic lymph node metastasis in patients without clinical lateral pelvic lymph node metastasis (clinical stage II/III): The analysis of data from the clinical trial (JCOG0212)

European Journal of Surgical Oncology ◽

10.1016/j.ejso.2018.11.016 ◽

2019 ◽

Vol 45 (3) ◽

pp. 336-340 ◽

Cited By ~ 7

Author(s):

Koji Komori ◽

Shin Fujita ◽

Junki Mizusawa ◽

Yukihide Kanemitsu ◽

Masaaki Ito ◽

...

Keyword(s):

Clinical Trial ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Predictive Factors ◽

Clinical Stage ◽

Pelvic Lymph Node ◽

Stage Ii ◽

Lateral Pelvic Lymph Node ◽

Node Metastasis ◽

Pelvic Lymph Node Metastasis

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Breast Cancer Combined with Contralateral Neck Lymph Node Metastasis. Case Report

10.21203/rs.3.rs-917470/v1 ◽

2021 ◽

Author(s):

Xiaoxiao Zhong ◽

Fengjiao Ding ◽

Liyuan Qian ◽

Wei Wu ◽

Yanguang Wen ◽

...

Keyword(s):

Breast Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Clinical Stage ◽

Pathological Examination ◽

Cervical Lymph Nodes ◽

Neck Lymph Node ◽

Node Metastasis ◽

Neck Lymph Node Metastasis ◽

The Right

Abstract Background: Contralateral neck lymph node metastasis is rare for primary breast cancer. Its clinical stage and treatment principles are lack of authoritative guidelines. A 30-year-old breast cancer patient with contralateral neck lymph node metastasis is presented. The clinical treatment is discussed combined with current research.Case presentation: A 30-year-old woman presented with a right breast mass for 5 months and left neck lymph node enlargement for 5 days. The mammography showed a 33mm*14.3mm mass in the inner quadrant of right breast. The ultrasound showed several hypoechoic nodules on the left side of the neck. Rapid intraoperative pathological examination diagnosed right breast malignant tumor and poorly differentiated carcinoma of the left cervical lymph nodes. Then the right mastectomy was performed immediately. The patient was scheduled to administer chemotherapy, molecular targeted therapy, radiotherapy and endocrinotherapy after operation. The long-term efficacy remains to be seen.Conclusion: The infrequent presentation of breast cancer with metastasis to the contralateral neck lymph node can be challenged for standard therapies.

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Downregulated circular RNA itchy E3 ubiquitin protein ligase correlates with advanced pathologic T stage, high lymph node metastasis risk and poor survivals in prostate cancer patients

Cancer Biomarkers ◽

10.3233/cbm-182111 ◽

2019 ◽

Vol 26 (1) ◽

pp. 41-50 ◽

Cited By ~ 5

Author(s):

Enying Huang ◽

Xiaogang Chen ◽

Yuan Yuan

Keyword(s):

Prostate Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Cancer Patients ◽

Circular Rna ◽

Ubiquitin Protein Ligase ◽

Node Metastasis ◽

T Stage ◽

High Lymph Node

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Computed Tomography Histogram Approach to Predict Lymph Node Metastasis in Patients With Clinical Stage IA Lung Cancer

The Annals of Thoracic Surgery ◽

10.1016/j.athoracsur.2019.04.082 ◽

2019 ◽

Vol 108 (4) ◽

pp. 1021-1028

Author(s):

Yoshihisa Shimada ◽

Yujin Kudo ◽

Hideyuki Furumoto ◽

Kentaro Imai ◽

Sachio Maehara ◽

...

Keyword(s):

Computed Tomography ◽

Lung Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Clinical Stage ◽

Node Metastasis ◽

Clinical Stage Ia ◽

Stage Ia

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Assessment of non‐lobe‐specific lymph node metastasis in clinical stage IA non‐small cell lung cancer

Thoracic Cancer ◽

10.1111/1759-7714.13121 ◽

2019 ◽

Vol 10 (7) ◽

pp. 1597-1604 ◽

Cited By ~ 2

Author(s):

Zhirong Zhang ◽

Jinbai Miao ◽

Qirui Chen ◽

Yili Fu ◽

Hui Li ◽

...

Keyword(s):

Lung Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Small Cell Lung Cancer ◽

Clinical Stage ◽

Small Cell ◽

Small Cell Lung ◽

Node Metastasis ◽

Clinical Stage Ia ◽

Stage Ia

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Frequency of Lympnode Metastasis in Supraglottic Ca Larynx

Medicine Today ◽

10.3329/medtoday.v31i2.41954 ◽

2019 ◽

Vol 31 (2) ◽

pp. 72-75

Author(s):

Mohammad Rokan Uddin Bhuiyan ◽

Mohammad Idris Ali ◽

Balayet Hossain Siddiquee ◽

Fatema Johora ◽

Mohammad Kamal Hossain

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Cervical Lymphadenopathy ◽

Female Ratio ◽

Node Metastasis ◽

T Stage ◽

Supraglottic Carcinoma ◽

Level Ii ◽

Number Of Patients ◽

Carcinoma Of Larynx

Introduction: To observe the association between the level of lymph node metastasis and the T- stage and to evaluate the N- stage in supraglottic carcinoma of larynx. Materials and Methods: A prospective cross-sectional study was carried out on 80 consecutive cases of supraglottic carcinoma larynx in three tertiary level hospital in Dhaka during July'2009 to March 2011. Results: The highest number of patients were in the age group 5th and 6th decades. Male to female ratio was 9:1. Topographically 70 cases (87.5%) had lesions involving epiglottis, 5 cases (6.25%) in aryepiglottic fold and 5 cases (6.25%) had lesions at false cord. Majority cases were histopathologically confirmed squamous cell carcinoma (95%) and among them 46 cases (57.5%) had well differentiated. Cervical metastatic lymph nodes were found 40% (32 cases) where level-II was most common. This was most frequent in early supraglottic(T1&T2) carcinoma of larynx (86.25%), P=0.008. A positive correlation was found between the T stage of primary tumours and frequency of cervical lymphadenopathy. Lymph node metastasis were 4.77% in T1, 37.50% in T2, 71.43% in T3 and 91.67% in T4. In early stage lymph node involved 93.75% in Level- II, 6.25% in Level- III & in advanced stage 100% in Level - II, 62.5% in Level - III and 25.0% in Level - IV. Distribution of study cases (31.25%) in stage-II, 25% in stage-I, 22.50% in stage-Ill and 21.25% were in stage-IV. Conclusion: Result of this study may help the clinician for planning of treatment of this malignant diseases as well awareness. Medicine Today 2019 Vol.31(2): 72-75

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Cervical invasion, lymphovascular space invasion, and ovarian metastasis as predictors of lymph node metastasis and poor outcome on stages I to III endometrial cancers: a single-center retrospective study

World Journal of Surgical Oncology ◽

10.1186/s12957-019-1733-2 ◽

2019 ◽

Vol 17 (1) ◽

Author(s):

Min Li ◽

Shuwei Wu ◽

Yangqin Xie ◽

Xiaohui Zhang ◽

Zhanyu Wang ◽

...

Keyword(s):

Overall Survival ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Regression Models ◽

Cox Regression ◽

Myometrial Invasion ◽

Ovarian Metastasis ◽

Surgical Staging ◽

Tumor Diameter ◽

Node Metastasis

Abstract Background The aim of this study is to determine pathological factors that increase the risk of LNM and indicate poor survival of patients diagnosed with endometrial cancer and treated with surgical staging. Method Between January 2010 and November 2018, we enrolled 874 eligible patients who received staging surgery in the First Affiliated Hospital of Anhui Medical University. The roles of prognostic risk factors, such as age, histological subtype, tumor grade, myometrial infiltration, tumor diameter, cervical infiltration, lymphopoiesis space invasion (LVSI), CA125, and ascites, were evaluated. Multivariable logistic regression models were used to identify the predictors of LNM. Kaplan–Meier and COX regression models were utilized to study the overall survival. Results Multivariable regression analysis confirmed cervical stromal invasion (OR 3.412, 95% CI 1.631–7.141; P < 0.01), LVSI (OR 2.542, 95% CI 1.061–6.004; P = 0.04) and ovarian metastasis (OR 6.236, 95% CI 1.561–24.904; P = 0.01) as significant predictors of nodal dissemination. Furthermore, pathological pattern (P = 0.03), myometrial invasion (OR 2.70, 95% CI 1.139–6.40; P = 0.01), and lymph node metastasis (OR 9.675, 95% CI 3.708–25.245; P < 0.01) were independent predictors of decreased overall survival. Conclusions Cervical invasion, lymphopoiesis space invasion, and ovarian metastasis significantly convey the risk of LNM. Pathological type, myometrial invasion, and lymph node metastasis are all important predictors of survival and should be scheduled for completion when possible in the surgical staging procedure.

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