Shock test of marine diesel engine is the important content for ship anti-shock research. Plentiful shock tests of equipments have been carried out abroad, but there is no detailed test methods of diesel engine. According to simulation results, 8-channel acceleration test points are determined. Because diesel engine is working, the measured shock acceleration is interfered by vibration signal. Orthogonal wavelet decomposition and wavelet noise reduction methods are used to separate shock component from test results. The seperated shock component consists of two parts. One is the low-frequency part caused by the shock from diesel foundation and then attenuation through the isolator, the other is the high-frequency part caused by the secondary shock of the retainer.