Electrochemical Biosensors for Bioprocess Control

2020 ◽  
pp. 173-192
Author(s):  
Robert J. Geise ◽  
Alexander M. Yacynych
Keyword(s):  
Electrochemical Biosensors ◽  
Bioprocess Control

Electrochemical Biosensors as a Screening Tool of In Vitro DNA-Drug Interaction

2005 ◽  
Vol 1 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Graziana Bagni ◽  
Mauro Ravera ◽  
Domenico Osella ◽  
Marco Mascini
Keyword(s):  
Drug Interaction ◽  
Screening Tool ◽  
Electrochemical Biosensors

scholarly journals The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 246
Author(s):  
Patrick Severin Sfragano ◽  
Giulia Moro ◽  
Federico Polo ◽  
Ilaria Palchetti
Keyword(s):  
Functional Groups ◽  
Synthetic Peptides ◽  
Clinical Diagnostics ◽  
Electrochemical Biosensors ◽  
Practical Application ◽  
Design And Development ◽  
Electrode Surfaces ◽  
Electrochemical Transducers ◽  
Target Analyte

Peptides represent a promising class of biorecognition elements that can be coupled to electrochemical transducers. The benefits lie mainly in their stability and selectivity toward a target analyte. Furthermore, they can be synthesized rather easily and modified with specific functional groups, thus making them suitable for the development of novel architectures for biosensing platforms, as well as alternative labelling tools. Peptides have also been proposed as antibiofouling agents. Indeed, biofouling caused by the accumulation of biomolecules on electrode surfaces is one of the major issues and challenges to be addressed in the practical application of electrochemical biosensors. In this review, we summarise trends from the last three years in the design and development of electrochemical biosensors using synthetic peptides. The different roles of peptides in the design of electrochemical biosensors are described. The main procedures of selection and synthesis are discussed. Selected applications in clinical diagnostics are also described.

scholarly journals Electrochemical Biosensors for Cytokine Profiling: Recent Advancements and Possibilities in the Near Future

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 94
Author(s):  
Nirmita Dutta ◽  
Peter B. Lillehoj ◽  
Pedro Estrela ◽  
Gorachand Dutta
Keyword(s):  
Immune System ◽  
Cost Effective ◽  
Anomalous Behavior ◽  
Quantitative Detection ◽  
Electrochemical Biosensors ◽  
Aberrant Expression ◽  
Detection Techniques ◽  
Short Period ◽  
User Friendly ◽  
Cytokine Profiling

Cytokines are soluble proteins secreted by immune cells that act as molecular messengers relaying instructions and mediating various functions performed by the cellular counterparts of the immune system, by means of a synchronized cascade of signaling pathways. Aberrant expression of cytokines can be indicative of anomalous behavior of the immunoregulatory system, as seen in various illnesses and conditions, such as cancer, autoimmunity, neurodegeneration and other physiological disorders. Cancer and autoimmune diseases are particularly adept at developing mechanisms to escape and modulate the immune system checkpoints, reflected by an altered cytokine profile. Cytokine profiling can provide valuable information for diagnosing such diseases and monitoring their progression, as well as assessing the efficacy of immunotherapeutic regiments. Toward this goal, there has been immense interest in the development of ultrasensitive quantitative detection techniques for cytokines, which involves technologies from various scientific disciplines, such as immunology, electrochemistry, photometry, nanotechnology and electronics. This review focusses on one aspect of this collective effort: electrochemical biosensors. Among the various types of biosensors available, electrochemical biosensors are one of the most reliable, user-friendly, easy to manufacture, cost-effective and versatile technologies that can yield results within a short period of time, making it extremely promising for routine clinical testing.

scholarly journals Label-Free Electrochemical Test of Protease Interaction with a Peptide Substrate Modified Gold Electrode

Chemosensors ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 199
Author(s):  
Anna Wcisło ◽  
Izabela Małuch ◽  
Paweł Niedziałkowski ◽  
Tadeusz Ossowski ◽  
Adam Prahl
Keyword(s):  
Gold Electrode ◽  
High Selectivity ◽  
Electrode Materials ◽  
Electrochemical Biosensors ◽  
Electrochemical Characteristics ◽  
Label Free ◽  
Peptide Substrate ◽  
Electrochemical Test ◽  
Experimental Conditions ◽  
Spectrofluorimetric Method

Efficient deposition of biomolecules on the surface, maintaining their full activity and stability, is a most significant factor in biosensor construction. For this reason, more and more research is focused on the development of electrochemical biosensors that have the ability to electrically detect adsorbed molecules on electrode surface with high selectivity and sensitivity. The presented research aims to develop an efficient methodology that allows quantification of processes related to the evaluation of enzyme activity (proprotein convertase) using electrochemical methods. In this study we used impedance spectroscopy to investigate the immobilization of peptide substrate (Arg-Val-Arg-Arg) modified with 11-mercaptoundecanoic acid on the surface of gold electrode. Both the synthesis of the peptide substrate as well as the full electrochemical characteristics of the obtained electrode materials have been described. Experimental conditions, including concentration of peptide substrate immobilization, modification time, linker, and the presence of additional blocking groups have been optimized. The main advantages of the described method is that it makes it possible to observe the peptide substrate–enzyme interaction without the need to use fluorescent labels. This also allows observation of this interaction at a very low concentration. Both of these factors make this new technique competitive with the standard spectrofluorimetric method.

Development of Electrochemical Biosensors for Coronavirus Detection

2021 ◽  
pp. 187-206
Author(s):  
Fulden Ulucan‐Karnak ◽  
Cansu İ. Kuru ◽  
Zeynep Yilmaz‐Sercinoglu
Keyword(s):  
Electrochemical Biosensors

scholarly journals Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2940
Author(s):  
Antonella Curulli
Keyword(s):  
Food Industry ◽  
Electrochemical Sensors ◽  
Cost Effective ◽  
Electrochemical Biosensors ◽  
Food Chains ◽  
Sensing Systems ◽  
Highly Sensitive ◽  
Skilled Personnel ◽  
Recent Developments ◽  
Key Issues

Safety and quality are key issues for the food industry. Consequently, there is growing demand to preserve the food chain and products against substances toxic, harmful to human health, such as contaminants, allergens, toxins, or pathogens. For this reason, it is mandatory to develop highly sensitive, reliable, rapid, and cost-effective sensing systems/devices, such as electrochemical sensors/biosensors. Generally, conventional techniques are limited by long analyses, expensive and complex procedures, and skilled personnel. Therefore, developing performant electrochemical biosensors can significantly support the screening of food chains and products. Here, we report some of the recent developments in this area and analyze the contributions produced by electrochemical biosensors in food screening and their challenges.

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