Abstract
Photosystem II (PS II)-inhibitor herbicide resistance in Ontario waterhemp [Amaranthus tuberculatus (Moq.) Sauer] population is conferred via target-site resistance (TSR) and non-target-site resistance (NTSR) mechanisms. Metribuzin-resistant (MR) A. tuberculatus is due to TSR,. Conversely, in other populations of PS II-resistant A. tuberculatus, plants are resistant to atrazine but metribuzin-sensitive (MS). The objective of this study was to determine the biologically-effective-dose of metribuzin applied PRE and POST for the control of MS and MR A. tuberculatus. Ten field experiments were conducted in 2019 and 2020 to determine the effective doses of metribuzin for 50, 80, and 95% control of MS and MR A. tuberculatus. Metribuzin applied PRE at the calculated doses of 133, 350, and 1070 g ai ha-1 controlled MS A. tuberculatus 50, 80, and 95%, respectively, whereas the calculated doses of 7868 and 17533 g ai ha-1 controlled MR A. tuberculatus 50 and 80%, respectively at 12 WAA. Metribuzin applied POST at the calculated doses of 245 and 1480 g ai ha-1 controlled MS A. tuberculatus 50 and 80%, respectively; the calculated dose for 50% MR A. tuberculatus control was greater than the highest dose (17920 g ai ha-1) included in this study. Metribuzin at 560 and 1120 g ha-1 and pyroxasulfone/flumioxazin (240 g ai ha-1) applied PRE controlled MS A. tuberculatus 88, 95, and 98%, respectively at 12 WAA. The aforementioned treatments controlled MR A. tuberculatus 0, 4, and 93%, respectively at 12 WAA. Metribuzin at 560 and 1120 g ha-1 and fomesafen (240 g ai ha-1) applied POST controlled MS A. tuberculatus 65, 70, and 78%, and MR A. tuberculatus 0, 1, and 49%, respectively at 12 WAA. Based on these results, NTSR PS II-resistant A. tuberculatus (enhanced metabolism) is controlled with metribuzin applied PRE and POST, in contrast TSR PS II-resistant A. tuberculatus (glycine264serine altered target site) is not controlled with metribuzin.