The Dietary Polyphenol Metabolite Enterolactone Activates Estrogen Signaling in Reproductive and Non-Reproductive Tissues and Regulates the Expression of Circadian Genes in Mice

2011 ◽  
pp. OR34-3-OR34-3
Author(s):  
Pauliina Damdimopoulou ◽  
Tarja Nurmi ◽  
Anu Salminen ◽  
Maria Yli-Heikkila ◽  
Anastasios E Damdimopoulos ◽  
...  
Keyword(s):  
Estrogen Signaling ◽  
Circadian Genes ◽  
Reproductive Tissues

Regulation of the Oxytocin Receptor in Bovine Reproductive Tissues and the Role of Steroids

2000 ◽  
Vol 35 (3-4) ◽  
pp. 134-141 ◽  
Author(s):  
R Ivell ◽  
A-R Fuchs ◽  
R Bathgate ◽  
G Tillmann ◽  
T Kimura
Keyword(s):  
Oxytocin Receptor ◽  
Reproductive Tissues

Identification of vitamin D (VDR) and retinoic X (RXR) receptor in normal and neoplastic human reproductive tissues

2013 ◽  
Author(s):  
Federica Cariati ◽  
Vincenzo Gigantino ◽  
Giorgio Coppola ◽  
Claudia Pivonello ◽  
Mariano Galdiero ◽  
...  
Keyword(s):  
Vitamin D ◽  
Reproductive Tissues ◽  
Human Reproductive

Membrane estrogen receptor α is essential for estrogen signaling in the male skeleton

2018 ◽  
Vol 239 (3) ◽  
pp. 303-312 ◽  
Author(s):  
H H Farman ◽  
K L Gustafsson ◽  
P Henning ◽  
L Grahnemo ◽  
V Lionikaite ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Α ◽  
Estrogen Signaling ◽  
Areal Bone Mineral Density ◽  
Male Mice ◽  
Mineral Density ◽  
Intact Male ◽  
Trabecular Thickness ◽  
Total Body

The importance of estrogen receptor α (ERα) for the regulation of bone mass in males is well established. ERα mediates estrogenic effects both via nuclear and membrane-initiated ERα (mERα) signaling. The role of mERα signaling for the effects of estrogen on bone in male mice is unknown. To investigate the role of mERα signaling, we have used mice (Nuclear-Only-ER; NOER) with a point mutation (C451A), which results in inhibited trafficking of ERα to the plasma membrane. Gonadal-intact male NOER mice had a significantly decreased total body areal bone mineral density (aBMD) compared to WT littermates at 3, 6 and 9 months of age as measured by dual-energy X-ray absorptiometry (DEXA). High-resolution microcomputed tomography (µCT) analysis of tibia in 3-month-old males demonstrated a decrease in cortical and trabecular thickness in NOER mice compared to WT littermates. As expected, estradiol (E2) treatment of orchidectomized (ORX) WT mice increased total body aBMD, trabecular BV/TV and cortical thickness in tibia compared to placebo treatment. E2 treatment increased these skeletal parameters also in ORX NOER mice. However, the estrogenic responses were significantly decreased in ORX NOER mice compared with ORX WT mice. In conclusion, mERα is essential for normal estrogen signaling in both trabecular and cortical bone in male mice. Increased knowledge of estrogen signaling mechanisms in the regulation of the male skeleton may aid in the development of new treatment options for male osteoporosis.

Influence of cellular metabolites on RNA and protein syntheses by Xanthium nuclei of different developmental phases

1986 ◽  
Vol 64 (12) ◽  
pp. 2922-2927
Author(s):  
A. Jana ◽  
S. P. Sen
Keyword(s):  
Rna Synthesis ◽  
Supernatant Fraction ◽  
Xanthium Strumarium ◽  
Low Molecular Weight ◽  
Transcriptional Level ◽  
Protein Patterns ◽  
Rna Molecules ◽  
Reproductive Tissues ◽  
Developmental Phases ◽  
Vegetative Leaf

Leaf nuclei of vegetative and reproductive plants of Xanthium strumarium L. were incubated with the postribosomal supernatant of either phase and changes at the transcriptional level were studied in homologous and heterologous combinations. In the presence of the supernatant of reproductive plants, RNA synthesis by vegetative nuclei was decreased by 25%. Reproductive nuclei were less active in RNA synthesis. Gel electrophoretic studies revealed four RNA bands in vegetative nuclei incubated with reproductive supernatant, including a fast-moving low molecular weight band that could not be detected when the "vegetative" supernatant was used. The adenine/uracil ratios of the newly synthesized RNA of vegetative nuclei treated with vegetative and reproductive supernatants were 1.46 and 1.54, respectively, compared with 1.15 and 1.04 in the reproductive nuclei. Competitive DNA–RNA hybridization experiments indicated that about 2% of the [3H]RNA synthesized by nuclei of vegetative plants in the presence of the supernatant of reproductive plants could not be beaten out by the RNA of vegetative plants. Small quantitative differences, thus, may be expected in the RNA molecules synthesized by nuclei in the presence of the supernatant fraction of vegetative and reproductive plants. The supernatant fraction of the reproductive tissues decreased the incorporation of [3H]alanine and [3H]leucine in both the buffer-soluble and acid-soluble proteins and the nuclei of vegetative plants were more active in protein synthesis. Protein patterns as studied by acrylamide gel electrophoresis revealed alterations when vegetative leaf nuclei were incubated with the supernatant of reproductive tissues.

scholarly journals Regulatory Interplay between miR-181a-5p and Estrogen Receptor Signaling Cascade in Breast Cancer

Cancers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 543
Author(s):  
Rosaria Benedetti ◽  
Chiara Papulino ◽  
Giulia Sgueglia ◽  
Ugo Chianese ◽  
Tommaso De Marchi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Endocrine Therapy ◽  
Mirna Expression ◽  
Estrogen Receptor Alpha ◽  
Estrogen Signaling ◽  
Integrated Analysis ◽  
Tumor Resistance ◽  
Estrogen Receptor Signaling

The efficacy and side effects of endocrine therapy in breast cancer (BC) depend largely on estrogen receptor alpha (ERα) expression, the specific drug administered, and treatment scheduling. Although the benefits of endocrine therapy outweigh any adverse effects in the initial stages of BC, later- or advanced-stage tumors acquire resistance to treatments. The mechanisms underlying tumor resistance to therapy are still not well understood, posing a major challenge for BC patient care. Epigenetic regulation and miRNA expression may be involved in the switch from a treatment-sensitive to a treatment-resistant state and could provide a valid therapeutic strategy for ERα negative BC. Here, a hybrid lysine-specific histone demethylase inhibitor, MC3324, displaying selective estrogen receptor down-regulator-like activities in BC, was used to highlight the interplay between epigenetic and ERα signaling. MC3324 anticancer action is mediated by microRNA (miRNA) expression regulation, indicating an innovative function for this molecule. Integrated analysis suggests a crosstalk between estrogen signaling, ERα interactors, miRNAs, and their putative targets. Specifically, miR-181a-5p expression is regulated by MC3324 and has an impact on cellular levels of ERα. A comparison of breast tumor versus healthy mammary tissues confirmed the important role of miR-181a-5p in ERα regulation and points to its putative predictive function in BC therapy.

scholarly journals Early and late stage MPN patients show distinct gene expression profiles in CD34+ cells

2021 ◽  
Author(s):  
Julian Baumeister ◽  
Tiago Maié ◽  
Nicolas Chatain ◽  
Lin Gan ◽  
Barbora Weinbergerova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Late Stage ◽  
Rna Splicing ◽  
Bone Marrow Cells ◽  
Myeloproliferative Neoplasms ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Primary Myelofibrosis ◽  
Estrogen Signaling ◽  
Therapeutic Modalities

AbstractMyeloproliferative neoplasms (MPN), comprising essential thrombocythemia (ET), polycythemia vera (PV), and primary myelofibrosis (PMF), are hematological disorders of the myeloid lineage characterized by hyperproliferation of mature blood cells. The prediction of the clinical course and progression remains difficult and new therapeutic modalities are required. We conducted a CD34+ gene expression study to identify signatures and potential biomarkers in the different MPN subtypes with the aim to improve treatment and prevent the transformation from the rather benign chronic state to a more malignant aggressive state. We report here on a systematic gene expression analysis (GEA) of CD34+ peripheral blood or bone marrow cells derived from 30 patients with MPN including all subtypes (ET (n = 6), PV (n = 11), PMF (n = 9), secondary MF (SMF; post-ET-/post-PV-MF; n = 4)) and six healthy donors. GEA revealed a variety of differentially regulated genes in the different MPN subtypes vs. controls, with a higher number in PMF/SMF (200/272 genes) than in ET/PV (132/121). PROGENγ analysis revealed significant induction of TNFα/NF-κB signaling (particularly in SMF) and reduction of estrogen signaling (PMF and SMF). Consistently, inflammatory GO terms were enriched in PMF/SMF, whereas RNA splicing–associated biological processes were downregulated in PMF. Differentially regulated genes that might be utilized as diagnostic/prognostic markers were identified, such as AREG, CYBB, DNTT, TIMD4, VCAM1, and S100 family members (S100A4/8/9/10/12). Additionally, 98 genes (including CLEC1B, CMTM5, CXCL8, DACH1, and RADX) were deregulated solely in SMF and may be used to predict progression from early to late stage MPN. Graphical abstract

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