scholarly journals In Vitro Glycoxidized Low-Density Lipoproteins and Low-Density Lipoproteins Isolated from Type 2 Diabetic Patients Activate Platelets via p38 Mitogen-Activated Protein Kinase

2007 ◽  
Vol 92 (5) ◽  
pp. 1961-1964 ◽  
Author(s):  
Catherine Calzada ◽  
Laurent Coulon ◽  
Déborah Halimi ◽  
Elodie Le Coquil ◽  
Valérie Pruneta-Deloche ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
P38 Mapk ◽  
Low Density Lipoproteins ◽  
Thromboxane B2 ◽  
Diabetic Patients ◽  
Low Density ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

Abstract Context: Platelet hyperactivation contributes to the increased risk for atherothrombosis in type 2 diabetes and is associated with oxidative stress. Plasma low-density lipoproteins (LDLs) are exposed to both hyperglycemia and oxidative stress, and their role in platelet activation remains to be ascertained. Objective: The aim of this study was to investigate the effects of LDLs modified by both glycation and oxidation in vitro or in vivo on platelet arachidonic acid signaling cascade. The activation of platelet p38 MAPK, the stress kinase responsible for the activation of cytosolic phospholipase A2, and the concentration of thromboxane B2, the stable catabolite of the proaggregatory arachidonic acid metabolite thromboxane A2, were assessed. Results: First, in vitro-glycoxidized LDLs increased the phosphorylation of platelet p38 MAPK as well as the concentration of thromboxane B2. Second, LDLs isolated from plasma of poorly controlled type 2 diabetic patients stimulated both platelet p38 MAPK phosphorylation and thromboxane B2 production and possessed high levels of malondialdehyde but normal α-tocopherol concentrations. By contrast, LDLs from sex- and age-matched healthy volunteers had no activating effects on platelets. Conclusions: Our results indicate that LDLs modified by glycoxidation may play an important contributing role in platelet hyperactivation observed in type 2 diabetes via activation of p38 MAPK.

Myricetin May Provide Protection against Oxidative Stress in Type 2 Diabetic Erythrocytes

2009 ◽  
Vol 64 (9-10) ◽  
pp. 626-630 ◽  
Author(s):  
Kanti Bhooshan Pandey ◽  
Neetu Mishra ◽  
Syed Ibrahim Rizvi
Keyword(s):  
Oxidative Stress ◽  
Biological Effects ◽  
Protein Carbonyl ◽  
In Vivo Studies ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Protein Carbonyl Content ◽  
Type 2 Diabetic

Oxidative stress is believed to be a major contributing factor in the development of late complications of diabetes. Many in vitro and in vivo studies have demonstrated that several parameters of red blood cell function and integrity are negatively affected by increased oxidative stress. Plant polyphenols are reported to exert many biological effects due to their antioxidant property. The present study was undertaken to evaluate the antioxidant effect of myricetin on markers of oxidative stress in erythrocytes from type 2 diabetic patients. The study was carried out on blood samples obtained from 23 type 2 diabetic patients and 23 age-matched control subjects. Erythrocytes were subjected to in vitro oxidative stress by incubating with 10-5 M tert-butyl hydroperoxide (t-BHP). Erythrocyte membrane lipid peroxidation and protein oxidation were measured in terms of malondialdehyde (MDA) and protein carbonyl group levels. The results showed an elevated MDA and protein carbonyl content in diabetic erythrocytes which were further increased after incubation with t-BHP. Myricetin at micromolar concentration significantly (p < 0.01) protected an t-BHP-induced increase in levels of oxidative stress parameters of diabetic erythrocytes

Enhanced susceptibility of low-density lipoprotein to in vitro oxidation in type 1 and type 2 diabetic patients

1995 ◽  
Vol 239 (2) ◽  
pp. 131-141 ◽  
Author(s):  
Jean-Louis Beaudeux ◽  
Pierre-Jean Guillausseau ◽  
Jacqueline Peynet ◽  
Françoise Flourie ◽  
Michel Assayag ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Patients ◽  
Low Density ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

scholarly journals Association of APOB 3ʹ-VNTR alleles with type 2 diabetes, BMI, systolic and diastolic blood pressure

2018 ◽  
Vol 17 (1) ◽  
pp. 71-77
Author(s):  
Abu Ashfaqur Sajib ◽  
Mohammad Abu Taher Khan ◽  
Mohammad Nasimul Haque ◽  
KM Kaderi Kibria ◽  
Abul Kalam Azad Chowdhury ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Medical Science ◽  
Gallstone Formation ◽  
Low Density Lipoproteins ◽  
Diabetic Patients ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

Background: Apolipoprotein B (APOB) is a component of chylomicrons, low-density lipoproteins (LDL), very low-density lipoproteins (VLDL), intermediate density lipoproteins (IDL) and functions as the main protein for transporting cholesterol to peripheral cells. APOB gene has an AT-rich VNTR site at its 3ʹ-untranslated region (3ʹ-UTR). APOB 3ʹ-VNTR alleles with ≥36 repeats have been shown to be strongly associated with increased serum lipid levels, gallstone formation and coronary artery disease.Objectives: To investigate any possible association of APOB 3ʹ-VNTR alleles with type 2 diabetes, BMI, systolic and diastolic blood pressure.Materials and methods: APOB 3ʹ-VNTR region in the DNA of non-diabetic controls and type 2 diabetic patients were amplified by polymerase chain reaction (PCR) and the numbers of core repeat in the amplified products were determined. Frequencies of the APOB alleles and genotypes among the controls and the patients were calculated and statistical analyses were performed.Results and discussion: Here we report for the first time that APOB 3ʹ-VNTR alleles have different distribution frequencies among type 2 diabetic and non-diabetic individuals. We also observed higher body mass index (BMI), systolic and diastolic blood pressures in individuals who had at least one APOB 3ʹ-VNTR allele with ≥35 repeats.Conclusion: Our study might bridge among the genetic signature of APOB 3ʹ-VNTR, high APOB protein level in blood, diabetes and other co-morbidities.Bangladesh Journal of Medical Science Vol.17(1) 2018 p.71-77

scholarly journals Study the action of pycnogenol on glucose and lipid profile in type -2- diabetic patients

2019 ◽  
Vol 17 (72) ◽  
pp. 35-46
Author(s):  
Rasha Zuhair ◽  
Dr.Amar Maola Hmod
Keyword(s):  
Glucose Level ◽  
Biochemical Parameters ◽  
Low Density Lipoproteins ◽  
Control Group ◽  
Diabetic Patients ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

Pycnogenol  pinus pinaster Ait ( a water extract of polyphenolic compounds ) was extracted from Iraqi pine bark and analyzed using high performance liquid chromotography (HPLC) coupled to ultra violet UV detection that was recorded at (254 nm).            Pycnogenol action on some biochemical parameters (glucose , total cholesterol (TC) , triacylglycerol (TG) , high density lipoproteins (HDL) ,low density lipoproteins (LDL) , very low density lipoproteins (VLDL) )level was determined in (30) type -2- diabetic patients who treated with capsules (500 mg) of pycnogenol three times daily to examine the reactive role of pycnogenol against oxidative stress.The above biochemical parameters were measured in plasma before treatment with pycnogenol and after (1 week) , (2 weeks) and (3 weeks) of treatment with this polyphenolic extract.            Our results have shown that  glucose level was increased relatively for diabetic patients compared with control group (p<0.001) but after treatment with pycnogenol , glucose level would be decreased relatively (p<0.001) after (1 week) , (p<0.001) after (2 weeks) and (p<0.05) after (3 weeks) of treatment with pycnogenol.Total cholesterol (TC) level increased relatively for diabetic patients compared with control group (p<0.001) , but after treatment with this reactive extract  (TC) level  decreased relatively (p<0.001) after (1 week) , (p<0.001) after (2 weeks) and (p<0.01) after (3 weeks) of treatment with pycnogenol.Triacylglycerol (TG) level was  increased relatively for diabetic patients compared with control group (p<0.001) , but after treatment with this antioxidant  extract  (TG) level decreased relatively (p<0.001) after (1 week) , (p<0.001) after (2 weeks) and (p<0.01) after (3 weeks) of treatment with pycnogenol.High density lipoproteins (HDL) level was decreased relatively for diabetic patients compared with control group (p<0.001) , but after treatment with pycnogenol (HDL) level increased relatively (p<0.01) after (1 week) , (p<0.01) after (2 weeks) and (p<0.05) after (3 weeks) of treatment with pycnogenol.Low density lipoproteins (LDL) level increased relatively for diabetic patients compared with control group (p<0.001) , but after treatment with  pycnogenol , (LDL) level decreased relatively (p<0.001) after (1 week) , (p<0.001) after (2 weeks) and (p<0.01) after (3 weeks) of treatment with pycnogenol. Very low density lipoproteins (VLDL) level increased relatively for diabetic patients compared with control group (p<0.001) , but after treatment with  pycnogenol , (VLDL) level decreased relatively (p<0.001) after (1 week) , (p<0.001) after (2 weeks) and (p<0.01) after (3 weeks) of treatment with pycnogenol.

Role of Oxidative Stress and Mitochondrial Dysfunction in Skeletal Muscle in Type 2 Diabetic Patients

2016 ◽  
Vol 22 (18) ◽  
pp. 2650-2656 ◽  
Author(s):  
Noelia Diaz-Morales ◽  
Susana Rovira-Llopis ◽  
Irene Escribano-Lopez ◽  
Celia Bañuls ◽  
Sandra Lopez-Domenech ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Mitochondrial Dysfunction ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

scholarly journals Soy Milk Consumption, Inflammation, Coagulation, and Oxidative Stress Among Type 2 Diabetic Patients With Nephropathy

Diabetes Care ◽  
2012 ◽  
Vol 35 (10) ◽  
pp. 1981-1985 ◽  
Author(s):  
M. S. Miraghajani ◽  
A. Esmaillzadeh ◽  
M. M. Najafabadi ◽  
M. Mirlohi ◽  
L. Azadbakht
Keyword(s):  
Oxidative Stress ◽  
Diabetic Patients ◽  
Milk Consumption ◽  
Soy Milk ◽  
Type 2 Diabetic Patients ◽  
And Oxidative Stress ◽  
Type 2 Diabetic

scholarly journals Lipid Profile of Type 2 Diabetic and Hypertensive Patients in the Jamaican Population

2010 ◽  
Vol 2 (01) ◽  
pp. 025-030 ◽  
Author(s):  
Lorenzo Gordon ◽  
Dalip Ragoobirsingh ◽  
Errol Y St A Morrison ◽  
Eric Choo-Kang ◽  
Donovan McGrowder ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Diabetic Patients ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Type 2 Diabetic Patients ◽  
Type 2 Dm ◽  
Type 2 Diabetic

ABSTRACT Aims: Previous studies have shown that diabetes mellitus (DM) increases the risk of cardiovascular diseases in females to a greater extent than in males. In this cross-sectional study, we evaluated the lipid profiles of type 2 diabetic males and females. Materials and Methods: The study included 107 type 2 diabetic patients (41 males and 66 females), and 122 hypertensive type 2 diabetic patients (39 males and 83 females), aged 15 years and older. Total cholesterol (TC), triglycerides (TG), low density lipoprotein-cholesterol (LDL-C), very low density lipoprotein-cholesterol (VLDL-C) and high density lipoprotein-cholesterol (HDL-C) concentrations were assayed for each group using standard biochemical methods. Results: The mean TC, TG, VLDL-C, HDL-C and LDL-C concentrations, TG/HDL and LDL/HDL ratios were higher in type 2 diabetic and hypertensive type 2 diabetic patients compared with non-diabetic, and hypertensive non-diabetic control subjects, although these were not significant (P > 0.05). Hypertensive type 2 diabetic females had significantly higher serum TC (7.42 ± 1.63 mmol/L) than hypertensive non-diabetic males (5.76±1.57 mmol/L; P < 0.05). All the other lipid and lipoprotein parameters except HDL-C were non-significantly higher in females with type 2 DM and those with hypertension and type 2 DM, compared with type 2 diabetic and hypertensive type 2 diabetic males, respectively (P > 0.05). Conclusion: This study demonstrated that dyslipidemia exists in our type 2 diabetic population with greater TC in hypertensive type 2 diabetic females compared with hypertensive type 2 diabetic males. This suggests that hypertensive type 2 diabetic females are exposed more profoundly to risk factors including atherogenic dyslipidemia compared with males.

scholarly journals Effect of atorvastatin on oxidative stress parameters and lipid profile in type 2 diabetic patients

2020 ◽  
Vol 7 (2) ◽  
Author(s):  
Najah RH ◽  
Mohammad AAH ◽  
Ammar RMR
Keyword(s):  
Oxidative Stress ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Oxidative Stress Parameters ◽  
Type 2 Diabetic ◽  
Stress Parameters

Introduction: Evidence has long existed regarding the relationship between oxidative stress and diabetes. The present study was conducted to assess the effect of atorvastatin on selected oxidative stress parameters in the form of reduced glutathione (GSH), lipid peroxidation byproduct malondialdehyde (MDA) levels, glutathione –S- transferase (GST) activity and catalase (CAT) activity) and its effect on lipid profile (total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) in dyslipidaemic type 2 diabetic patients . Materials and Methods: Fifty nine dyslipidaemic type 2 diabetic patients were included in this study. Full history was taken and general examination of patients was performed. Patients studied were taking glibenclamide (an oral hypoglycaemic drug) during the study as a treatment for their disease. These patients were followed up for 60 days and divided randomly into 2 groups. Group I (n = 31): no drug was given and served as dyslipidaemic diabetic control. Group II (n = 28): received atorvastatin tablets 20 mg once daily at night. Of the 59 Fifty patients, 46 completed the study while 13 patients withdrew. This is due to non compliance of the patients. Blood samples were drawn from the patients at the beginning and after 60 days of follow up between 8:30 & 10:30 am after at least 12-14 hours fast. Fasting blood glucose, lipid profile, selected oxidative stress parameters (GSH, MDA levels, GST and CAT activities) were measured. Renal and hepatic functions were also assessed. Results: This study revealed that: atorvastatin treatment increased serum GSH; reduced MDA levels significantly while did not significantly affect CAT and GST activity. In atorvastatin treatment, TC, TG, LDL and VLDL decreased significantly while HDL increased significantly. Conclusion: There was insignificant correlations between atorvastatin induced changes in the oxidation markers and the observed changes of the lipid profile.

A pilot study to detect oxidative stress in type 2 diabetic patients

2016 ◽  
Vol 96 ◽  
pp. S62
Author(s):  
Ayse C. Hamamcioglu ◽  
Zehra Safi-Oz ◽  
Yasin Hazer ◽  
Dilek Arpaci ◽  
Furuzan Kokturk
Keyword(s):  
Oxidative Stress ◽  
Pilot Study ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic
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