Muscarinic, Ca++antagonist and specific butyrylcholinesterase inhibitory activity of dried ginger extract might explain its use in dementia

Escherichia coli is a gram negative bacterium which is a normal flora in the digestive tract. In birds, this bacterium causes a disease known as colibasillosis. Antibiotics are generally used, but excessive use will cause residues and antibiotic resistance. To avoid resistance or residue, an alternative treatment is needed. The combination of propolis and ginger extract is very promising to develop because both have a synergistic effect as antimicrobials. The research aims to determine the effect of the combination extract on Escherichia coli bacteria in vitro. We used 8 groups which contain combinations of propolis and ginger extract. We used amoxicillin disk as a positive control, 1% Na CMC as a negative control. The results showed a combination of ginger and propolis extract showed good inhibitory activity against Escherichia coli in all groups> 6mm., and the highest inhibitory activity was K3 (5% propolis combination and 15% ginger extract) with 8.7 mm. The combination of propolis and ginger extract has the potential to be used as an alternative antibiotic to prevent antibiotic resistance from synthetic antibiotics.Keywords: alternative antibiotics, combination extracts, Escherichia coli, ginger, in vitro, propolis

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Combinations of herbs and probiotics as an alternative growth promoter: An in vitro study

Veterinary World ◽

10.14202/vetworld.2019.614-620 ◽

2019 ◽

Vol 12 (4) ◽

pp. 614-620 ◽

Cited By ~ 5

Author(s):

Vinsa Cantya Prakasita ◽

Widya Asmara ◽

Sitarina Widyarini ◽

Agnesia Endang Tri Hastuti Wahyuni

Keyword(s):

Inhibitory Activity ◽

Inhibitory Effect ◽

Herbal Extract ◽

Diffusion Method ◽

Growth Promoter ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Ginger Extract ◽

Adhesion Ability ◽

Red Ginger

Background and Aim: Antibiotic growth promoters (AGPs) are added to animal feed to stimulate growth and increase livestock productivity. However, the regular use of antibiotics in animal diets has a considerable contribution to the occurrence of antibiotic resistance in livestock and humans. This study aimed to investigate the feasibility of red ginger (Zingiber officinale var. Rubrum), turmeric (Curcuma domestica), and wild ginger (Curcuma xanthorrhiza), Lactobacillus acidophilus, and Lactobacillus brevis as an alternative to AGPs. Materials and Methods: The antibacterial activities and probiotic stimulatory effects of herbs were screened through the disc diffusion method and optical densitometry. The inhibitory ability of probiotics against pathogens was also tested through the disc diffusion method. The adhesion ability of probiotics was tested by mixing the optimal herbal combinations with broiler intestinal epithelial cells (105 cells/ml). The cells were then subjected to Gram staining, and the number of adherent bacteria was calculated. Results: The test results showed that 3.13% ethanolic wild ginger extract had the highest inhibitory activity against Salmonella Enteritidis, followed by ethanolic red ginger extract and aqueous wild ginger extract at the same concentration. The three extracts also supported the growth of L. acidophilus and L. brevis. Further tests showed that the combination of 3.13% ethanolic red ginger extract had the highest inhibitory activity against S. Enteritidis, followed by ethanolic and aqueous wild ginger extract at the same concentration. The three extracts also supported the growth of L. acidophilus and L. brevis. Further tests showed that the combination of 3.13% ethanolic red ginger extract and 3.13% aqueous wild ginger extract had the best inhibitory effect on the growth of S. Enteritidis. The stimulatory effect of the combinations of herbal extract on the growth of L. acidophilus (0.18±0.00) and L. brevis (0.21±0.01) was better than those of individual extract, positive controls, and the glucose control. L. acidophilus and L. brevis had a weak inhibitory effect on the growth of S. Enteritidis (<6 mm). The adhesion ability of L. acidophilus (420.00±28.21) and L. brevis (259.33±24.03) was stronger than that of S. Enteritidis (202.00±14.00) under treatment with combined extracts. Conclusion: The tested combinations of herbs and probiotics can adhere to the intestinal tract. Given this characteristic, herb and probiotic combinations may be developed as an alternative to conventional AGPs.

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Muscarinic, Ca++ antagonist and specific butyrylcholinesterase inhibitory activity of dried ginger extract might explain its use in dementia

Journal of Pharmacy and Pharmacology ◽

10.1211/jpp/60.10.0014 ◽

2008 ◽

Vol 60 (10) ◽

pp. 1375-1383 ◽

Cited By ~ 4

Author(s):

Muhammad N. Ghayur ◽

Anwarul H. Gilani ◽

Touqeer Ahmed ◽

Asaad Khalid ◽

Sarfraz A. Nawaz ◽

...

Keyword(s):

Inhibitory Activity ◽

Ginger Extract

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Enterohepatic recirculation of bioactive ginger phytochemicals is associated with enhanced tumor growth-inhibitory activity of ginger extract

Carcinogenesis ◽

10.1093/carcin/bgu011 ◽

2014 ◽

Vol 35 (6) ◽

pp. 1320-1329 ◽

Cited By ~ 28

Author(s):

Sushma R. Gundala ◽

Rao Mukkavilli ◽

Chunhua Yang ◽

Pooja Yadav ◽

Vibha Tandon ◽

...

Keyword(s):

Tumor Growth ◽

Inhibitory Activity ◽

Enterohepatic Recirculation ◽

Ginger Extract ◽

Growth Inhibitory ◽

Growth Inhibitory Activity

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Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000652 ◽

2020 ◽

pp. 1-9

Author(s):

Pınar Ercan ◽

Sedef Nehir El

Keyword(s):

Inhibitory Activity ◽

Digestive Enzymes ◽

Positive Control ◽

Anthocyanin Content ◽

Inhibitory Effects ◽

Total Anthocyanin ◽

Total Anthocyanin Content ◽

Before And After ◽

Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

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