Involvement of RBP-J in biological functions of mouse Notch1 and its derivatives

Development ◽  
1997 ◽  
Vol 124 (20) ◽  
pp. 4133-4141 ◽  
Author(s):  
H. Kato ◽  
Y. Taniguchi ◽  
H. Kurooka ◽  
S. Minoguchi ◽  
T. Sakai ◽  
...  
Keyword(s):  
Cell Fate ◽  
Mutant Cell ◽  
Precursor Cells ◽  
Physical Interaction ◽  
Binding Motifs ◽  
Cell Lineages ◽  
Transactivation Activity ◽  
Vp16 Fusion ◽  
Myogenic Precursor

Notch is involved in the cell fate determination of many cell lineages. The intracellular region (RAMIC) of Notch1 transactivates genes by interaction with a DNA binding protein RBP-J. We have compared the activities of mouse RAMIC and its derivatives in transactivation and differentiation suppression of myogenic precursor cells. RAMIC comprises two separate domains, IC for transactivation and RAM for RBP-J binding. Although the physical interaction of IC with RBP-J was much weaker than with RAM, transactivation activity of IC was shown to involve RBP-J by using an RBP-J null mutant cell line. IC showed differentiation suppression activity that was generally comparable to its transactivation activity. The RBP-J-VP16 fusion protein, which has strong transactivation activity, also suppressed myogenesis of C2C12. The RAM domain, which has no other activities than binding to RBP-J, synergistically stimulated transactivation activity of IC to the level of RAMIC. The RAM domain was proposed to compete with a putative co-repressor for binding to RBP-J because the RAM domain can also stimulate the activity of RBP-J-VP16. These results taken together, indicate that differentiation suppression of myogenic precursor cells by Notch signalling is due to transactivation of genes carrying RBP-J binding motifs.

Development of early cell lineages in marsupial embryos: an overview

1994 ◽  
Vol 6 (4) ◽  
pp. 507 ◽  
Author(s):  
L Selwood
Keyword(s):  
Cell Fate ◽  
Three Dimensional ◽  
Embryonic Cell ◽  
Cleavage Pattern ◽  
Cell Lineages ◽  
Trophectoderm Cells ◽  
Maternal Determinants ◽  
Virginia Opossum ◽  
Do So

All major embryonic and extra-embryonic cell lineages are established before implantation in marsupials. In reptiles, birds, monotremes and most marsupials, the zygote is polarized, sometimes markedly so, and the cleavage pattern in relation to the polarized state provides the mechanism for the generation of positional signals. These ensure that the embryonic cell lineages develop in the centre of the developing blastoderm or blastocyst epithelium and the extra-embryonic lineages at the periphery. The evolution of the vertebrate yolky egg was accompanied by a decreasing dependence on maternal determinants and increasing dependence on positional signals to determine cell fate. It is proposed that when a less yolky egg evolved, the mechanisms for determination of cell fate in a developing epithelium were retained. It is proposed that in marsupials, positional signals are involved in the determination of cell fate of embryonic and trophectoderm cells but do so in a two-dimensional epithelium not a three-dimensional morula. The next lineage formed is the primary endoderm which separates off from the primitive ectoderm in the embryoblast and eventually lines the blastocyst cavity. Positional signals are responsible for the determination of primary endoderm in eutherian mammals, birds and probably also marsupials. Order of cell division during cleavage provides a mechanism whereby some cells in the embryoblast of marsupials have earlier and greater contact with their neighbouring cells. The mechanism for determination of primary endoderm cells in the blastocyst epithelium is examined in the Virginia opossum and the stripe-faced dunnart.

Primary culture of single ectodermal precursors of Drosophila reveals a dorsoventral prepattern of intrinsic neurogenic and epidermogenic capabilities at the early gastrula stage

Development ◽  
1992 ◽  
Vol 116 (2) ◽  
pp. 377-385 ◽  
Author(s):  
K. Luer ◽  
G.M. Technau
Keyword(s):  
Cell Fate ◽  
Precursor Cells ◽  
Drosophila Embryo ◽  
Original Position ◽  
Division Pattern ◽  
Dorsal Ectoderm ◽  
Development In Vitro ◽  
Almost All

We have analyzed the development in vitro of individual precursor cells from the presumptive truncal segmental ectoderm of the Drosophila embryo to study the intrinsic component in the determination of cell fate. For each cultured cell, the original position within as well as the developmental stage of the donor embryo were known. Cells removed from the ventral neurogenic region develop neural clones. Cells from the dorsal ectoderm and from the dorsalmost part of the ventral neurogenic ectoderm develop epidermal clones. These two classes of clones differ with respect to their division pattern, adhesiveness, cell morphologies and the expression of cell-specific markers. Mixed neural/epidermal clones were obtained from a fraction of precursors at almost all dorsoventral sites. We conclude that, at the onset of gastrulation, precursor cells of the truncal segmental ectoderm already have the capability to develop as either neuroblasts or epidermoblasts in the absence of further cell interactions. At the same time, positional cues distributed along the dorsoventral axis equip precursors with intrinsic preferences towards the neural or epidermal fate, thus defining a prepattern of high neurogenic preferences ventrally, and high epidermogenic preferences dorsally. It is likely that this prepattern is involved in defining the extent of the ventral neurogenic and dorsal epidermogenic regions of the ectoderm. The roles of intrinsic capabilities versus extrinsic influences in the regulation of the characteristic pattern of segregation of the two lineages are discussed.

scholarly journals The EGL-13 SOX Domain Transcription Factor Affects the Uterine Cell Lineages in Caenorhabditis elegans

Genetics ◽  
2003 ◽  
Vol 165 (3) ◽  
pp. 1623-1628
Author(s):  
Hediye Nese Cinar ◽  
Keri L Richards ◽  
Kavita S Oommen ◽  
Anna P Newman
Keyword(s):  
Transcription Factor ◽  
Cell Division ◽  
Caenorhabditis Elegans ◽  
Cell Fate ◽  
Cell Lineages

Abstract We isolated egl-13 mutants in which the cells of the Caenorhabditis elegans uterus initially appeared to develop normally but then underwent an extra round of cell division. The data suggest that egl-13 is required for maintenance of the cell fate.

Cell lineage of homeotic mutants of Drosophila

1985 ◽  
Vol 312 (1153) ◽  
pp. 139-144 ◽  
Keyword(s):  
Mode Of Action ◽  
Cell Lineage ◽  
Precursor Cells ◽  
Larval Period ◽  
Homeotic Genes ◽  
Lineage Analysis

The homeotic genes specify the development of specific groups of precursor cells. They establish the correct state of determination of the different primordia. Cell lineage analysis has been particularly useful in studying the mode of action of homeotic genes. The main findings are: (i) most, perhaps all, the homeotic genes are required by every cell of the corresponding primordium (that is, they are cell autonomous); (ii) they act on anatomical units defined by compartment boundaries and including one or more compartments, (iii) most, but not all, homeotic genes are required until the end of the larval period; (iv) the homeotic genes act in combination so that the appropriate development of a given primordium may be established by the contribution of several homeotic genes.

MyoD and Myf-5 define the specification of musculature of distinct embryonic origin

1998 ◽  
Vol 76 (6) ◽  
pp. 1079-1091 ◽  
Author(s):  
Boris Kablar ◽  
Atsushi Asakura ◽  
Kirsten Krastel ◽  
Chuyan Ying ◽  
Linda L May ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Expression Pattern ◽  
Branchial Arch ◽  
Precursor Cells ◽  
Mouse Development ◽  
Unique Role ◽  
Branchial Arches ◽  
Embryonic Origin ◽  
Body Wall Muscles ◽  
Myogenic Precursor

Mounting evidence supports the notion that Myf-5 and MyoD play unique roles in the development of epaxial (originating in the dorso-medial half of the somite, e.g. back muscles) and hypaxial (originating in the ventro-lateral half of the somite, e.g. limb and body wall muscles) musculature. To further understand how Myf-5 and MyoD genes co-operate during skeletal muscle specification, we examined and compared the expression pattern of MyoD-lacZ (258/-2.5lacZ and MD6.0-lacZ) transgenes in wild-type, Myf-5, and MyoD mutant embryos. We found that the delayed onset of muscle differentiation in the branchial arches, tongue, limbs, and diaphragm of MyoD-/- embryos was a consequence of a reduced ability of myogenic precursor cells to progress through their normal developmental program and not because of a defect in migration of muscle progenitor cells into these regions. We also found that myogenic precursor cells for back, intercostal, and abdominal wall musculature in Myf-5-/-embryos failed to undergo normal translocation or differentiation. By contrast, the myogenic precursors of intercostal and abdominal wall musculature in MyoD-/- embryos underwent normal translocation but failed to undergo timely differentiation. In conclusion, these observations strongly support the hypothesis that Myf-5 plays a unique role in the development of muscles arising after translocation of epithelial dermamyotome cells along the medial edge of the somite to the subjacent myotome (e.g., back or epaxial muscle) and that MyoD plays a unique role in the development of muscles arising from migratory precursor cells (e.g., limb and branchial arch muscles, tongue, and diaphragm). In addition, the expression pattern of MyoD-lacZ transgenes in the intercostal and abdominal wall muscles of Myf-5-/- and MyoD-/- embryos suggests that appropriate development of these muscles is dependent on both genes and, therefore, these muscles have a dual embryonic origin (epaxial and hypaxial).Key words: epaxial and hypaxial muscle, Myf-5, MyoD, mouse development, somite.

scholarly journals Determination of cell fate selection during phage lambda infection

2008 ◽  
Vol 105 (52) ◽  
pp. 20705-20710 ◽  
Author(s):  
F. St-Pierre ◽  
D. Endy
Keyword(s):  
Cell Fate ◽  
Phage Lambda

L3/Lhx8 is involved in the determination of cholinergic or GABAergic cell fate

2005 ◽  
Vol 94 (3) ◽  
pp. 723-730 ◽  
Author(s):  
T. Manabe ◽  
K. Tatsumi ◽  
M. Inoue ◽  
H. Matsuyoshi ◽  
M. Makinodan ◽  
...  
Keyword(s):  
Cell Fate
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