Regeneration in the sponge Sycon ciliatum partly mimics postlarval development

ABSTRACTSomatic cells dissociated from an adult sponge can reorganize and develop into a juvenile-like sponge, a remarkable phenomenon of regeneration. However, the extent to which regeneration recapitulates embryonic developmental pathways has remained enigmatic. We have standardized and established a sponge Sycon ciliatum regeneration protocol from dissociated cells. Morphological analysis demonstrated that dissociated sponge cells follow a series of morphological events resembling postembryonic development. We performed high-throughput sequencing on regenerating samples and compared the data with that from regular postlarval development. Our comparative transcriptomic analysis revealed that sponge regeneration is as equally dynamic as embryogenesis. We found that sponge regeneration is orchestrated by recruiting pathways similar to those utilized in embryonic development. We also demonstrated that sponge regeneration is accompanied by cell death at early stages, revealing the importance of apoptosis in remodelling the primmorphs to initiate re-development. Because sponges are likely to be the first branch of extant multicellular animals, we suggest that this system can be explored to study the genetic features underlying the evolution of multicellularity and regeneration.

Download Full-text

Regeneration in sponge Sycon ciliatum mimics postlarval development

10.1101/2020.05.04.061218 ◽

2020 ◽

Author(s):

Anael Soubigou ◽

Ethan G Ross ◽

Yousef Touhami ◽

Nathan Chrismas ◽

Vengamanaidu Modepalli

Keyword(s):

Cell Culture ◽

Signaling Pathways ◽

High Throughput ◽

Morphological Analysis ◽

High Throughput Sequencing ◽

Regulatory Mechanisms ◽

Somatic Development ◽

Developmental Signaling ◽

Genetic Features ◽

Comparative Transcriptomic Analysis

AbstractSomatic cells dissociated from an adult sponge can re-organize and develop into a functional juvenile. However, the extent to which regeneration recapitulates embryonic developmental signaling pathways has remained enigmatic for more than a century. To this end, we have standardized and established a sponge Sycon ciliatum regeneration protocol to achieve consistent regeneration in cell culture. From the morphological analysis, we demonstrated that dissociated sponge cells follow a series of morphological events resembling embryonic and postlarval development. Hence, we propose that sponge regeneration represents somatic development. To support our hypothesis, we performed high-throughput sequencing on regenerating samples and compared the data with regular embryonic and postlarval development of Sycon ciliatum. Our comparative transcriptomic analysis illuminates that sponge regeneration is equally as dynamic as embryogenesis. We find that sponge regeneration is orchestrated by complex regulatory mechanisms by recruiting signaling pathways like those utilized in embryonic development to organize into a functional juvenile. In the current study, we lay down the basic framework to study Sycon ciliatum regeneration. Since sponges are likely to be the first branch of extant multicellular animal and the sister lineage to nearly all animals, we suggest that this system can be explored to study the genetic features underlying the evolution of multicellularity and regeneration.

Download Full-text

High-throughput sequencing reveals distinct genetic features and clinical implications of NSCLC with de novo and acquired EGFR T790M mutation

Lung Cancer ◽

10.1016/j.lungcan.2018.08.014 ◽

2018 ◽

Vol 124 ◽

pp. 205-210 ◽

Cited By ~ 7

Author(s):

Panwen Tian ◽

Ye Wang ◽

Weiya Wang ◽

Yalun Li ◽

Ke Wang ◽

...

Keyword(s):

High Throughput ◽

High Throughput Sequencing ◽

De Novo ◽

Clinical Implications ◽

T790m Mutation ◽

Genetic Features ◽

Egfr T790m

Download Full-text

Differences in small noncoding RNAs profile between bull X and Y sperm

PeerJ ◽

10.7717/peerj.9822 ◽

2020 ◽

Vol 8 ◽

pp. e9822

Author(s):

Hao Zhou ◽

Jiajia Liu ◽

Wei Sun ◽

Rui Ding ◽

Xihe Li ◽

...

Keyword(s):

Functional Analysis ◽

Sex Differences ◽

Embryonic Development ◽

High Throughput ◽

High Throughput Sequencing ◽

Noncoding Rnas ◽

Mature Oocyte ◽

Small Noncoding Rnas ◽

Nucleosomal Dna ◽

Nucleosome Binding

The differences in small noncoding RNAs (sncRNAs), including miRNAs, piRNAs, and tRNA-derived fragments (tsRNAs), between X and Y sperm of mammals remain unclear. Here, we employed high-throughput sequencing to systematically compare the sncRNA profiles of X and Y sperm from bulls (n = 3), which may have a wider implication for the whole mammalian class. For the comparison of miRNA profiles, we found that the abundance of bta-miR-652 and bta-miR-378 were significantly higher in X sperm, while nine miRNAs, including bta-miR-204 and bta-miR-3432a, had greater abundance in Y sperm (p < 0.05). qPCR was then used to further validate their abundances. Subsequent functional analysis revealed that their targeted genes in sperm were significantly involved in nucleosome binding and nucleosomal DNA binding. In contrast, their targeted genes in mature oocyte were significantly enriched in 11 catabolic processes, indicating that these differentially abundant miRNAs may trigger a series of catabolic processes for the catabolization of different X and Y sperm components during fertilization. Furthermore, we found that X and Y sperm showed differences in piRNA clusters distributed in the genome as well as piRNA and tsRNA abundance, two tsRNAs (tRNA-Ser-AGA and tRNA-Ser-TGA) had lower abundance in X sperm than Y sperm (p < 0.05). Overall, our work describes the different sncRNA profiles of X and Y sperm in cattle and enhances our understanding of their potential roles in the regulation of sex differences in sperm and early embryonic development.

Download Full-text

Whole-genome sequencing reveals oncogenic mutations in mycosis fungoides

Blood ◽

10.1182/blood-2014-11-611194 ◽

2015 ◽

Vol 126 (4) ◽

pp. 508-519 ◽

Cited By ~ 112

Author(s):

Laura Y. McGirt ◽

Peilin Jia ◽

Devin A. Baerenwald ◽

Robert J. Duszynski ◽

Kimberly B. Dahlman ◽

...

Keyword(s):

Cell Death ◽

Whole Genome Sequencing ◽

Effective Treatment ◽

Mycosis Fungoides ◽

Genome Sequencing ◽

High Throughput ◽

High Throughput Sequencing ◽

Whole Genome ◽

Oncogenic Mutations ◽

Key Points

Key Points High-throughput sequencing of MF revealed multiple mutations within epigenetic and cytokine pathways that may drive disease. Pharmacologically targeting the JAK3 pathway in MF results in cell death and may be an effective treatment of this disease.

Download Full-text