Differentiation potentiality of rat visceral yolk sac in organ culture

Development ◽  
1984 ◽  
Vol 80 (1) ◽  
pp. 127-136
Author(s):  
Y. L. Lu ◽  
H. Sobis ◽  
L. Van Hove ◽  
M. Vandeputte
Keyword(s):  
Organ Culture ◽  
In Vitro Culture ◽  
Yolk Sac ◽  
Trophoblast Cells ◽  
Differentiated Cells ◽  
Visceral Yolk Sac ◽  
Poorly Differentiated

Visceral yolk sacs removed at day 12 of pregnancy in the rat were kept in organ culture for as long as 28 days. During this in vitro culture, proliferation of the endoderm and the mesoderm as well as of poorly differentiated cells was observed. The latter displayed neither the characteristics of endodermal nor mesodermal cells and their presence was frequently associated with the development of giant trophoblast cells. The hypothesis is proposed that these trophoblast cells originate from these poorly differentiated cells that acquire in vivo and in vitro the potentiality to differentiate.

Histochemical and autoradiographic study of the cultured rat visceral yolk sac

Development ◽  
1986 ◽  
Vol 97 (1) ◽  
pp. 169-176
Author(s):  
H. Sobis ◽  
J. Goebels ◽  
M. Vandeputte
Keyword(s):  
Organ Culture ◽  
Germ Cells ◽  
Autoradiographic Study ◽  
Yolk Sac ◽  
Differentiated Cells ◽  
Proliferation And Differentiation ◽  
Before And After ◽  
Visceral Yolk Sac ◽  
Mesodermal Origin

The proliferation and differentiation potentiality of the rat visceral yolk sac was investigated both in organ culture and after grafting in vivo. Using alkaline phosphatase as a marker for germ cells, it was shown that these cells are absent in the 12-day-old visceral yolk sac examined before and after organ culture. Therefore, the only cells that proliferate and differentiate must be of endodermal and/or mesodermal origin. By labelling the cells with [3H]thymidine both the endodermal and mesodermal cells were found to proliferate. After 10 days in organ culture or implantation in vivo differentiated tissues (e.g. squamous epidermis, endodermal cysts and giant trophoblast cells) were regularly detected. Several of these differentiated cells contained the radiolabel indicating that they derived from the initial proliferating endodermal and/or mesodermal cells.

Cellular events during early formation of yolk-sac-derived teratomas

Development ◽  
1982 ◽  
Vol 70 (1) ◽  
pp. 225-240
Author(s):  
H. Sobis ◽  
L. Van Hove ◽  
M. Vandeputte
Keyword(s):  
Stem Cells ◽  
Granulation Tissue ◽  
Morphological Study ◽  
Yolk Sac ◽  
Blastema Formation ◽  
Differentiated Cells ◽  
Cellular Events ◽  
Visceral Yolk Sac ◽  
Poorly Differentiated ◽  
Similarities And Differences

A sequential morphological study of the initial cellular events in teratoma induction by displaced visceral yolk sac after foetectomy in rats was undertaken. This study led to the observation that apart from proliferation of cells displaying definite endodermal or mesodermal characteristics,a population of poorly differentiated cells appeared some days after the surgical procedure. It is very likely that these poorly differentiated cells are stem cells from which differentiated structures originate afterwards by a process of redifferentiation. The development of granulation tissue rich in capillaries seems to enhance this process. Similarities and differences with blastema formation are discussed.

scholarly journals Multiangle light-scattering analysis of murine teratocarcinoma cells.

1979 ◽  
Vol 27 (1) ◽  
pp. 366-370 ◽  
Author(s):  
D E Swartzendruber ◽  
B J Price ◽  
L B Rall
Keyword(s):  
Stem Cells ◽  
Light Scattering ◽  
Flow System ◽  
Yolk Sac ◽  
Squamous Cells ◽  
Teratocarcinoma Cells ◽  
Visceral Yolk Sac ◽  
Yolk Sac Cells

Stem cells of the mouse testicular teratocarcinoma are capable of giving rise in vivo and in vitro to a wide variety of cell and tissue types representative of each embryonic germ layer. Multiangle light-scattering measurements in a flow system have been made on these stem cells and on a variety of their differentiated derivatives. This technique is capable of distinguishing the stem cells from parietal yolk sac cells, visceral yolk sac cells, neuronal cells and squamous cells. However, multipotential stem cells cannot be distinguished from stem cells that are restricted in their development to a single pathway.

scholarly journals Quantitative studies of pinocytosis. I. Kinetics of uptake of (125I)polyvinylpyrrolidone by rat yolk sac cultured in vitro.

1975 ◽  
Vol 64 (1) ◽  
pp. 113-122 ◽  
Author(s):  
K E Williams ◽  
E M Kidston ◽  
F Beck ◽  
J B Lloyd
Keyword(s):  
Electron Microscopy ◽  
In Vitro Culture ◽  
Quantitative Study ◽  
Light And Electron Microscopy ◽  
Yolk Sac ◽  
Quantitative Studies ◽  
Visceral Yolk Sac ◽  
Kinetics Of Uptake ◽  
Kinetics Of

A method is described for the in vitro culture of 17.5-day rat visceral yolk sac. Tissue survival was good as judged by light and electron microscopy. The rate of pinocytic uptake of 125I-labeled polyvinylpyrrolidone by the tissue was constant both within and between experiments. Within the concentration range 0.15-24 mug/ml, the 125I-labeled polyvinylpyrrolidone neither stimulated nor inhibited pinocytosis. The system offers many advantages in the quantitative study of the physical basis of pinocytosis.

scholarly journals Morphometric analysis of the visceral yolk sac endoderm in the rat in vivo and in vitro

Reproduction ◽  
1982 ◽  
Vol 65 (1) ◽  
pp. 239-245 ◽  
Author(s):  
M. Gupta ◽  
A.P. Gulamhusein ◽  
F. Beck
Keyword(s):  
Morphometric Analysis ◽  
Yolk Sac ◽  
Visceral Yolk Sac

Hepoxilin A, hydroxyepoxide metabolite of arachidonic acid, stimulates transport of 45Ca across the guinea pig visceral yolk sac

1984 ◽  
Vol 62 (12) ◽  
pp. 1466-1469 ◽  
Author(s):  
L. O. Derewlany ◽  
C. R. Pace-Asciak ◽  
I. C. Radde
Keyword(s):  
Arachidonic Acid ◽  
Guinea Pig ◽  
Calcium Transport ◽  
Yolk Sac ◽  
Maximal Effect ◽  
Isolated Pancreatic Islets ◽  
Ussing Chambers ◽  
Visceral Yolk Sac

The effect of hepoxilin A, a newly isolated hydroxyepoxide metabolite of arachidonic acid, on calcium transport across the visceral yolk sac membrane of the guinea pig was investigated in vitro in Ussing chambers. While 1-14C-labelled hepoxilin A itself was not transported across the membrane, it increased the rate of transport of calcium toward the side to which hepoxilin A was added. The degree of increase in calcium transport was similar whether hepoxilin A was added to the maternal side or to the fetal side of the membrane. The observed effect was dependent on the concentration of hepoxilin A over a narrow range (0.5–1.0 × 10−6 M). It was also dependent on the time of incubation reaching maximal effect by 25 min. We have recently observed that hepoxilin A is formed from platelet-derived 12-hydroperoxyeicosatetraenoic acid (12-HPETE) through hernin and hemoglobin catalysis as well as during perifusion of 12-HPETE through isolated pancreatic islets. The present study suggests that hepoxilin A, if formed in vivo, could play a role in the mobilization of calcium.

scholarly journals Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts

2021 ◽  
Vol 22 (13) ◽  
pp. 6663
Author(s):  
Maurycy Jankowski ◽  
Mariusz Kaczmarek ◽  
Grzegorz Wąsiatycz ◽  
Claudia Dompe ◽  
Paul Mozdziak ◽  
...  
Keyword(s):  
Stem Cells ◽  
In Vitro Culture ◽  
Osteogenic Differentiation ◽  
Molecular Mechanisms ◽  
Marker Genes ◽  
P Value ◽  
Illumina Platform

Next-generation sequencing (RNAseq) analysis of gene expression changes during the long-term in vitro culture and osteogenic differentiation of ASCs remains to be important, as the analysis provides important clues toward employing stem cells as a therapeutic intervention. In this study, the cells were isolated from adipose tissue obtained during routine surgical procedures and subjected to 14-day in vitro culture and differentiation. The mRNA transcript levels were evaluated using the Illumina platform, resulting in the detection of 19,856 gene transcripts. The most differentially expressed genes (fold change >|2|, adjusted p value < 0.05), between day 1, day 14 and differentiated cell cultures were extracted and subjected to bioinformatical analysis based on the R programming language. The results of this study provide molecular insight into the processes that occur during long-term in vitro culture and osteogenic differentiation of ASCs, allowing the re-evaluation of the roles of some genes in MSC progression towards a range of lineages. The results improve the knowledge of the molecular mechanisms associated with long-term in vitro culture and differentiation of ASCs, as well as providing a point of reference for potential in vivo and clinical studies regarding these cells’ application in regenerative medicine.

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