Invasive behaviour of glioblastoma cell lines is associated with altered organisation of the cadherin-catenin adhesion system

2002 ◽  
Vol 115 (16) ◽  
pp. 3331-3340 ◽  
Author(s):  
Carla Perego ◽  
Cristina Vanoni ◽  
Silvia Massari ◽  
Andrea Raimondi ◽  
Sandra Pola ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Lines ◽  
Protein A ◽  
Cell Types ◽  
Glioblastoma Cell ◽  
Migration Assay ◽  
Adhesion System ◽  
Pdz Protein ◽  
Glioblastoma Cell Lines ◽  
Cell Cell

As little is known about the role of cadherin-mediated cell-cell adhesion in astrocytes and its alteration in migrating and invasive glioblastomas, we investigated its molecular composition and organisation in primary cultured astrocytes and the T98G and U373MG glioblastoma cell lines. Biochemical and morphological analysis indicated that all three cell types express all of the structural components of the adhesion system, including the LIN-7 PDZ protein,a novel component involved in the organisation of the junctional domain in epithelia and neurons. However, only the astrocytes and T98G cells generated and maintained mature adhesive junctional domains to which LIN-7 was recruited. Alterations in the junctional domain of U373MG cells were associated with higher motility in a poly-L-lysine migration assay. When the T98G cells were cultured on Matrigel matrix, they acquired invasive properties but, despite unchanged cadherin adhesion system protein levels, the invasive T98G cell-cell contacts failed to accumulate LIN-7 and failed to mature. These results identify the LIN-7 PDZ protein as a marker of cell adhesion maturity and cell invasion and indicate that instability and disorganisation of cadherin-mediated junctions rather than reduced expression of cadherin-catenin system components are required to promote migration and invasiveness in glioblastoma cell lines.

Impact of Zika virus infection in human glioblastoma cell lines.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e13563-e13563
Author(s):  
Andre P. Fay
Keyword(s):  
Cell Migration ◽  
Cell Adhesion ◽  
Cell Viability ◽  
Cell Lines ◽  
Zika Virus ◽  
Cell Infiltration ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell ◽  
Glioblastoma Cell Lines

e13563 Background: Glioblastoma (GBM) is the most common central nervous system (CNS) tumor. Despite available therapies survival remains poor, and new treatment strategies are needed. Oncolytic viral therapy is under investigation in GBM. Zika virus (ZV) infection has demonstrated inhibition of neuronal precursor cells proliferation in preclinical studies. This study aims to evaluate the effects of ZV infection on human glioblastoma cell lines survival. Methods: Two GBM cell lines (U138 and U 251) were infected by 2 hours with ZV using the titer of PFU/ml diluted in 1:100, 1:1000, 1: 10000. Cell. We performed tests to evaluate cell viability by MTT and protease active assay, cell migration trough a wound healing assay and cell infiltration using insert culture method. Also the cell lines were tested by cell adhesion capacity, apoptosis (Caspase 3/7), interleukins levels and cell surface markers for CD 14 and CD73.These results were compared to controls. Results: Our study has demonstrated a reduction in cell viability in U138 lineage trough MTT assay. In the U251 lineage and in the others tests for cytotoxicity/viability, ZK did not altered cell viability neither cell migration compared to controls. We showed that ZV caused reduction in cell invasion and resulted in increased rates of apoptosis in both cell lines. We observed that infection of ZV caused increased rates of cell adhesion and CD73 marker. Conclusions: These findings suggest that ZV infection may be associated with increased CD 73 Expression thus increasing cell adhesion and cell infiltration. The ZV may be cause an increase rates of apoptosis and influencing cell cytotoxicity and viability. Further investigations to explore the role of ZV in GBM treatment are warranted.

scholarly journals Two Cell Adhesion Molecules, Nectin and Cadherin, Interact through Their Cytoplasmic Domain–Associated Proteins

2000 ◽  
Vol 150 (5) ◽  
pp. 1161-1176 ◽  
Author(s):  
Kouichi Tachibana ◽  
Hiroyuki Nakanishi ◽  
Kenji Mandai ◽  
Kumi Ozaki ◽  
Wataru Ikeda ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Lines ◽  
Cell Adhesion Molecules ◽  
Cytoplasmic Domain ◽  
Adhesion Sites ◽  
Adhesion System ◽  
Associated Proteins ◽  
Mechanism Of Interaction ◽  
E Cadherin ◽  
Cell Cell

We have found a new cell–cell adhesion system at cadherin-based cell–cell adherens junctions (AJs) consisting of at least nectin and l-afadin. Nectin is a Ca2+-independent homophilic immunoglobulin-like adhesion molecule, and l-afadin is an actin filament-binding protein that connects the cytoplasmic region of nectin to the actin cytoskeleton. Both the trans-interaction of nectin and the interaction of nectin with l-afadin are necessary for their colocalization with E-cadherin and catenins at AJs. Here, we examined the mechanism of interaction between these two cell–cell adhesion systems at AJs by the use of α-catenin–deficient F9 cell lines and cadherin-deficient L cell lines stably expressing their various components. We showed here that nectin and E-cadherin were colocalized through l-afadin and the COOH-terminal half of α-catenin at AJs. Nectin trans-interacted independently of E-cadherin, and the complex of E-cadherin and α- and β-catenins was recruited to nectin-based cell–cell adhesion sites through l-afadin without the trans-interaction of E-cadherin. Our results indicate that nectin and cadherin interact through their cytoplasmic domain–associated proteins and suggest that these two cell–cell adhesion systems cooperatively organize cell–cell AJs.

scholarly journals Identification of a Drosophila homologue of alpha-catenin and its association with the armadillo protein.

1993 ◽  
Vol 121 (5) ◽  
pp. 1133-1140 ◽  
Author(s):  
H Oda ◽  
T Uemura ◽  
K Shiomi ◽  
A Nagafuchi ◽  
S Tsukita ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Lines ◽  
Chromosome Band ◽  
Cell Contact ◽  
Beta Catenin ◽  
Contact Sites ◽  
Adhesion System ◽  
A Cell ◽  
Drosophila Embryos ◽  
Cell Cell

The cadherin cell adhesion system plays a central role in cell-cell adhesion in vertebrates, but its homologues are not identified in the invertebrate. alpha-Catenins are a group of proteins associated with cadherins, and this association is crucial for the cadherins' function. Here, we report the cloning of a Drosophila alpha-catenin gene by low stringent hybridization with a mouse alpha E-catenin probe. Isolated cDNAs encoded a 110-kD protein with 60% identity to mouse alpha E-catenin, and this protein was termed D alpha-catenin. The gene of this protein was located at the chromosome band 80B. Immunostaining analysis using a mAb to D alpha-catenin revealed that it was localized to cell-cell contact sites, expressed throughout development and present in a wide variety of tissues. When this protein was immunoprecipitated from detergent extracts of Drosophila embryos or cell lines, several proteins co-precipitated. These included the armadillo product which was known to be a Drosophila homologue of beta-catenin, another cadherin-associated protein in vertebrates, and a 150-kD glycoprotein. These results strongly suggest that Drosophila has a cell adhesion machinery homologous to the vertebrate cadherin-catenin system.

FOTEMUSTINE EFFECT ON HUMAN GLIOBLASTOMA CELL LINES

Tsitologiya ◽  
2018 ◽  
Vol 60 (1) ◽  
Author(s):  
L. N. Kiseleva ◽  
◽  
A. V. Kartashev ◽  
N. L. Vartanyan ◽  
A. A. Pinevich ◽  
...  
Keyword(s):  
Cell Lines ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell ◽  
Glioblastoma Cell Lines

Adenosine inhibits cell proliferation differently in human astrocytes and in glioblastoma cell lines

Neuroscience ◽  
2021 ◽  
Author(s):  
Marcelino H ◽  
Carvalho TMA ◽  
Tomás J ◽  
Teles FI ◽  
Honório AC ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Glioblastoma Cell ◽  
Human Astrocytes ◽  
Glioblastoma Cell Lines

scholarly journals Irradiation differentially affects substratum-dependent survival, adhesion, and invasion of glioblastoma cell lines

2003 ◽  
Vol 89 (11) ◽  
pp. 2122-2132 ◽  
Author(s):  
N Cordes ◽  
B Hansmeier ◽  
C Beinke ◽  
V Meineke ◽  
D van Beuningen
Keyword(s):  
Cell Lines ◽  
Glioblastoma Cell ◽  
Adhesion And Invasion ◽  
Glioblastoma Cell Lines

scholarly journals Expression and putative functions of GDF-15, a member of the TGF-β superfamily, in human glioma and glioblastoma cell lines

2008 ◽  
Vol 270 (1) ◽  
pp. 30-39 ◽  
Author(s):  
Jens Strelau ◽  
Corina Schmeer ◽  
Heike Peterziel ◽  
Tina Sackmann ◽  
Christel Herold-Mende ◽  
...  
Keyword(s):  
Cell Lines ◽  
Glioblastoma Cell ◽  
Human Glioma ◽  
Glioblastoma Cell Lines

scholarly journals Differential response of human glioblastoma cell lines to combined camptothecin and ionizing radiation treatment

2008 ◽  
Vol 7 (3) ◽  
pp. 364-373 ◽  
Author(s):  
Cholpon S. Djuzenova ◽  
Teresa Güttler ◽  
Sabrina Berger ◽  
Astrid Katzer ◽  
Michael Flentje
Keyword(s):  
Ionizing Radiation ◽  
Cell Lines ◽  
Radiation Treatment ◽  
Differential Response ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell ◽  
Glioblastoma Cell Lines
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