An inhibitor of cell cohesion from axenically grown cells of the slime mould, Dictyostelium discoideum

1977 ◽  
Vol 28 (1) ◽  
pp. 107-116
Author(s):  
A.P. Swan ◽  
D.R. Garrod ◽  
D. Morris
Keyword(s):  
Molecular Weight ◽  
Biological Effects ◽  
Low Molecular Weight ◽  
Stationary Phase Culture ◽  
Present Evidence ◽  
Slime Mould ◽  
Heat Stable ◽  
Phase Culture ◽  
Weight Substance ◽  
Millipore Filters

Medium from a stationary phase culture of axenically grown D. discoideum cells contains an inhibitor of cohesion of log phase cells. The inhibitor is a heat-stable, low molecular weight substance. Its biological effects include inhibition of cohesion of aggregation-competent cells, of cells of other slime mould species, the blocking of development on Millipore filters and a reduction in adhesiveness of slime mould cells to glass. Present evidence suggests that the inhibitor may bind to the cell surface.

The presence of a heat-stable cytosolic factor in rat submandibular gland which activates Na+,K+-ATPase

1985 ◽  
Vol 63 (7) ◽  
pp. 774-777
Author(s):  
D. J. Pon ◽  
D. J. Stewart ◽  
A. K. Sen
Keyword(s):  
Molecular Weight ◽  
Submandibular Gland ◽  
Low Molecular Weight ◽  
Microsomal Preparation ◽  
Cytosolic Protein ◽  
Heat Stable ◽  
Weight Substance ◽  
Rat Submandibular Gland ◽  
Molecular Sieve Column ◽  
Dose Dependent

A low molecular weight substance is present in the soluble fraction of the rat submandibular gland which activates Na+,K+-ATPase. Varying amounts of cytosolic protein from rat submandibular gland were added to a heavy microsomal preparation of the same tissue. A dose-dependent activation of the Na+,K+-ATPase was observed, with a peak activation of approximately 82% occurring when 9.0 μg/mL of cytosolic protein was included in the assay. The activating factor is heat stable and soluble following heat treatment. The factor elutes at a molecular mass of 600 daltons as determined by molecular sieve column chromatography. Ultraviolet–visible scanning of the eluted material results in an absorbance at 210 nm, which is characteristic of a low molecular weight peptide.

STUDIES ON THE ISOLATION AND NATURE OF THE 'TERREGENS FACTOR'

1954 ◽  
Vol 32 (1) ◽  
pp. 400-406 ◽  
Author(s):  
M. O. Burton ◽  
F. J. Sowden ◽  
A. G. Lochhead
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Ferric Iron ◽  
Test Organism ◽  
The Other ◽  
Inorganic Salts ◽  
Low Molecular Weight ◽  
Straight Chain ◽  
Heat Stable ◽  
Growth Promoting

A procedure is described for the production and concentration of the 'terregens factor' (TF), a bacterial growth promoting substance synthesized by Arthrobacter pascens and essential for the growth of Arthrobacter terregens. From culture filtrates of A. pascens cultivated in a medium of inorganic salts and sucrose, concentrates of TF may be obtained that are active at 0.001 μgm. Per ml., heat stable and contain about 12.7% nitrogen. Acid hydrolysis yielded a number of amino acids, including glutamic acid, glycine, α–alanine, valine, leucine, proline, lysine, and arginine, as well as some unidentified compounds; however, TF does not appear to be a low molecular weight straight chain peptide.Although TF contains no iron, it combines readily with ferrous or ferric iron to form reddish-brown complexes with this metal. Activity for A. terregens is shown by certain iron containing complexes as hemin, coprogen, and ferrichrome. On the other hand none is shown by cytochrome or pulcherrimin; however, aspergillic acid, structurally related to the latter, possesses some growth promoting activity for the test organism.

A LOW MOLECULAR WEIGHT SUBSTANCE OBTAINED FROM SERUM WHICH HAS LUTEINIZING HORMONE LIKE ACTIVITY (“MINI LH”)

1976 ◽  
Vol 43 (6) ◽  
pp. 1410-1413 ◽  
Author(s):  
F.A. Leidenberger ◽  
D. Graesslin ◽  
H.J. Scheel ◽  
N. Hess ◽  
V. Lichtenberg ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Luteinizing Hormone ◽  
Low Molecular Weight ◽  
Weight Substance

scholarly journals Polyethylene glycol as marker in piglet diets with a high dry-matter content

1968 ◽  
Vol 22 (3) ◽  
pp. 515-526 ◽  
Author(s):  
M. J. Manners ◽  
D. E. Kidder
Keyword(s):  
Molecular Weight ◽  
Small Intestine ◽  
Polyethylene Glycol ◽  
Dry Matter ◽  
Matter Content ◽  
Low Molecular Weight ◽  
Dry Matter Content ◽  
Casein Diet ◽  
High Dry Matter ◽  
Weight Substance

1. Polyethylene glycol (PEG) of high molecular weight, used as a dietary marker, did not distribute uniformly in the water present in a high-casein diet as did glucose, a low-molecular-weight substance.2. This distribution effect also occurred with the contents of the stomach and, to a smaller extent, with the contents of the first quarter of the small intestine of piglets given such a diet.

A heat stable low molecular weight inhibitor of lysosomal cysteine proteinases in human serum

1983 ◽  
Vol 110 (2) ◽  
pp. 449-455 ◽  
Author(s):  
Francis Gauthier ◽  
Maurice Pagano ◽  
Frédéric Esnard ◽  
Henri Mouray ◽  
Robert Engler
Keyword(s):  
Molecular Weight ◽  
Human Serum ◽  
Low Molecular Weight ◽  
Cysteine Proteinases ◽  
Heat Stable
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