Preparation of Glycopolymer-Modified Gold Nanoparticles and a New Approach for a Lateral Flow Assay

2011 ◽  
Vol 84 (5) ◽  
pp. 466-470 ◽  
Author(s):  
Jin Ishii ◽  
Masayuki Toyoshima ◽  
Miyuki Chikae ◽  
Yuzuru Takamura ◽  
Yoshiko Miura
Keyword(s):  
Gold Nanoparticles ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
New Approach

Electrochemical studies of lateral flow assay test results for procalcitonin detection

2021 ◽  
Author(s):  
Yachana Gupta Gupta ◽  
Kalpana ◽  
Aditya Sharma Ghrera
Keyword(s):  
Gold Nanoparticles ◽  
Detection Limit ◽  
Qualitative Analysis ◽  
Test Line ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Quantitative Detection ◽  
Cyclic Voltammograms ◽  
Paper Strip ◽  
Electrochemical Studies

In this study, the lateral flow assay (LFA) has been developed for the detection of bacterial infection (BI) by specific biomarker procalcitonin (PCT), without a need for complicated instrumentations and technical expertise. For the development of the assay, gold nanoparticles (AuNP) and their conjugates with antibodies specific to the model antigen PCT are assessed. Polyclonal antibody (pAb) labelled with gold nanoparticles (AuNP) to obtain the AuNP-pAb complex and the specific monoclonal antibody (mAb) have been dropped at the test zone. This complex is placed over the conjugate line of the LFA strip. In the absence of PCT or the presence of other biomarkers, the test line remained colourless, which revealed the specificity of assay towards PCT among a pool of various analytes. Herein, observations have been made through two different platforms for quantitative and qualitative analysis for the detection of PCT biomarker. The qualitative analysis has been performed on the basis of appearance red color in the test band, while for quantitative analysis, a novel approach has been adopted. Herein, the nitrocellulose membrane (paper strip) is cut out from the LFA strip and used for electrochemical studies under similar solution conditions. Different paper strips presented different cyclic voltammograms (CV) that could be correlated to varying PCT concentrations captured at the test line of the paper strip. The qualitative detection limit for PCT using this LFA was determined to be 2 ng/ml and the quantitative detection limit was 1 ng/ml. The electrochemical response studies of the paper strip by CV technique revealed the sensitivity value of 0.695 mA ng/ml.

scholarly journals Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 209
Author(s):  
Sasima Chotithammakul ◽  
Michael B. Cortie ◽  
Dakrong Pissuwan
Keyword(s):  
Chronic Kidney Disease ◽  
Gold Nanoparticles ◽  
Test Line ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Analytical Performance ◽  
Detection Mechanism ◽  
Multiple Factors ◽  
20 Nm ◽  
Target Analyte

The sensitivity and reproducibility of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single- and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal relative to the 20 nm GNPs, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a model biomarker for confronting chronic kidney disease.

Catalytic hairpin assembly-assisted lateral flow assay for visual determination of microRNA-21 using gold nanoparticles

2019 ◽  
Vol 186 (9) ◽  
Author(s):  
Wenjing Wang ◽  
Axiu Nie ◽  
Zhicheng Lu ◽  
Jinjie Li ◽  
Mingbo Shu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Catalytic Hairpin Assembly

scholarly journals Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen

Sensors ◽  
2016 ◽  
Vol 16 (12) ◽  
pp. 2154 ◽  
Author(s):  
Dong Kim ◽  
Yong Kim ◽  
Seok Hong ◽  
Jinwoon Kim ◽  
Nam Heo ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Size Controlled

scholarly journals Silver and gold nanoparticles as multi-chromatic lateral flow assay probes for the detection of food allergens

2018 ◽  
Vol 411 (9) ◽  
pp. 1905-1913 ◽  
Author(s):  
Laura Anfossi ◽  
Fabio Di Nardo ◽  
Alida Russo ◽  
Simone Cavalera ◽  
Cristina Giovannoli ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Food Allergens ◽  
Silver And Gold Nanoparticles

Performance comparison of single and mixed-sized gold nanoparticles on straightforward lateral flow assay for albumin detection

2020 ◽  
Author(s):  
Sasima Chotithammakul ◽  
Dakrong Pissuwan
Keyword(s):  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Test Line ◽  
Testing Procedure ◽  
Performance Comparison ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Analytical Performance ◽  
Detection Mechanism ◽  
Multiple Factors

The sensitivity of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed, which affect the sensitivity and reproducibility of the assay testing procedure. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal amplification, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a biomarker for confronting chronic kidney disease.

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM).The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

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