Catalytic hairpin assembly-assisted lateral flow assay for visual determination of microRNA-21 using gold nanoparticles

2019 ◽  
Vol 186 (9) ◽  
Author(s):  
Wenjing Wang ◽  
Axiu Nie ◽  
Zhicheng Lu ◽  
Jinjie Li ◽  
Mingbo Shu ◽  
...  
2018 ◽  
Vol 185 (2) ◽  
Author(s):  
Juanzu Liu ◽  
Junyan Wang ◽  
Zhaohui Li ◽  
Hongmin Meng ◽  
Lin Zhang ◽  
...  

2011 ◽  
Vol 84 (5) ◽  
pp. 466-470 ◽  
Author(s):  
Jin Ishii ◽  
Masayuki Toyoshima ◽  
Miyuki Chikae ◽  
Yuzuru Takamura ◽  
Yoshiko Miura

2021 ◽  
Vol 9 (10) ◽  
pp. 3661-3671
Author(s):  
Xiaowei Cao ◽  
Yue Sun ◽  
Yu Mao ◽  
Menglin Ran ◽  
Yifan Liu ◽  
...  

A novel surface-enhanced Raman scattering-lateral flow assay strip in combination with catalytic hairpin assembly signal amplification has been developed for rapid and sensitive detection of miR-196a-5p and miR-31-5p associated with lung cancer.


2021 ◽  
Vol 11 (10) ◽  
pp. 2023-2029
Author(s):  
Wang Zhang ◽  
Yujun Zhou ◽  
Xiuli Xu ◽  
Yaping Tian ◽  
Chunyan Zhang

Liver disease is a great danger to human health. The determination of blood level of Cholyglycine acid (CG) is a vital biomarker for the assessment of liver function in clinic, which is contribute to the diagnosis of liver diseases. Thus, establishing accuracy, rapid and convenient method for the detection of glycolic acid is of great significance. In this study, a time-resolved-fluorescence (TRF) lateral flow assay for rapid detection of CG was development. The analytical detection limit (mean of zero-2 SD) was 0.06 μg/mL The method showed good linearity in the range of 0.2–40 g/mL and was not affected by biomolecules with similar structure to CG. The analytical mean recovery of control was between 90–110% and the imprecision of intra- and inter-assay of CVs was less than 10%. No significant matrix effect was observed in saline, serum, plasma or whole blood. A good correlation was found with the homogeneous enzyme immunoassay (HEIA) assay (slopes 1.0463, y-intercepts 0.2721 μg/mL, R = 0.989, n = 50, P < 0.001). The CG TRF analysis could provide reproducible and quantitative information about the state of liver in a few minutes, which is suitable for the detection of liver diseases in point-of-care-testing (POCT) conditions.


Author(s):  
Yachana Gupta Gupta ◽  
Kalpana ◽  
Aditya Sharma Ghrera

In this study, the lateral flow assay (LFA) has been developed for the detection of bacterial infection (BI) by specific biomarker procalcitonin (PCT), without a need for complicated instrumentations and technical expertise. For the development of the assay, gold nanoparticles (AuNP) and their conjugates with antibodies specific to the model antigen PCT are assessed. Polyclonal antibody (pAb) labelled with gold nanoparticles (AuNP) to obtain the AuNP-pAb complex and the specific monoclonal antibody (mAb) have been dropped at the test zone. This complex is placed over the conjugate line of the LFA strip. In the absence of PCT or the presence of other biomarkers, the test line remained colourless, which revealed the specificity of assay towards PCT among a pool of various analytes. Herein, observations have been made through two different platforms for quantitative and qualitative analysis for the detection of PCT biomarker. The qualitative analysis has been performed on the basis of appearance red color in the test band, while for quantitative analysis, a novel approach has been adopted. Herein, the nitrocellulose membrane (paper strip) is cut out from the LFA strip and used for electrochemical studies under similar solution conditions. Different paper strips presented different cyclic voltammograms (CV) that could be correlated to varying PCT concentrations captured at the test line of the paper strip. The qualitative detection limit for PCT using this LFA was determined to be 2 ng/ml and the quantitative detection limit was 1 ng/ml. The electrochemical response studies of the paper strip by CV technique revealed the sensitivity value of 0.695 mA ng/ml.


Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 209
Author(s):  
Sasima Chotithammakul ◽  
Michael B. Cortie ◽  
Dakrong Pissuwan

The sensitivity and reproducibility of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single- and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal relative to the 20 nm GNPs, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a model biomarker for confronting chronic kidney disease.


Sensors ◽  
2016 ◽  
Vol 16 (12) ◽  
pp. 2154 ◽  
Author(s):  
Dong Kim ◽  
Yong Kim ◽  
Seok Hong ◽  
Jinwoon Kim ◽  
Nam Heo ◽  
...  

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