Biological Effects of a Sulfated-Polysaccharide Isolated from the Marine Red Algae Champia feldmannii

Gelidium crinale, the red algae belonging to Geliaceae Gelidium, is a traditional edible and industrial alga in China. A sulfated polysaccharide (GNP) is successfully separated from Gelidium crinale by acid extraction and two-step column chromatography. Chemical analysis showed that the molecular weight of GNP was 25.8 kDa and the monosaccharide composition had the highest galactose content and confirmed the presence and content (16.5%) of sulfate by Fourier transform infrared spectroscopy (FT-IR) spectrometry as well as barium chloride-gelatin methods. In addition, the effect of GNP on lipopolysaccharide (LPS)-induced oxidative stress and inflammation in macrophages was also evaluated. The research results showed that GNP had fairly strong scavenging activities on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, hydroxyl radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and had Fe2+-chelating ability in a dose-dependent manner. At the same time, it significantly inhibits the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the production of pro-inflammatory cytokines in RAW 264.7 cells induced by LPS through blocking the mitogen-activated protein kinase (MAPK)/nuclear factor kappa beta (NF-κB) signaling pathway. These results indicate that GNP may be a latent component anti-inflammation in pharmaceutical and functional food industries.

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Sulfated-polysaccharide fraction extracted from red algae G racilaria birdiae ameliorates trinitrobenzenesulfonic acid-induced colitis in rats

Journal of Pharmacy and Pharmacology ◽

10.1111/jphp.12231 ◽

2014 ◽

Vol 66 (8) ◽

pp. 1161-1170 ◽

Cited By ~ 7

Author(s):

Tarcisio V. Brito ◽

José P. R. P. Neto ◽

Rafael S. Prudêncio ◽

Jalles A. Batista ◽

José S. C. Júnior ◽

...

Keyword(s):

Red Algae ◽

Sulfated Polysaccharide ◽

Trinitrobenzenesulfonic Acid ◽

Polysaccharide Fraction

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Sulfated polysaccharide from the red algae Gelidiella acerosa: Anticoagulant, antiplatelet and antithrombotic effects

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2020.05.012 ◽

2020 ◽

Vol 159 ◽

pp. 415-421 ◽

Cited By ~ 1

Author(s):

Francisco Diêgo da Silva Chagas ◽

Glauber Cruz Lima ◽

Valesca Ingrid Nobre dos Santos ◽

Luís Eduardo Castanheira Costa ◽

Willer Malta de Sousa ◽

...

Keyword(s):

Red Algae ◽

Sulfated Polysaccharide ◽

Gelidiella Acerosa ◽

Antithrombotic Effects

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Inhibitory activities of marine sulfated polysaccharides against SARS-CoV-2

Food & Function ◽

10.1039/d0fo02017f ◽

2020 ◽

Vol 11 (9) ◽

pp. 7415-7420 ◽

Cited By ~ 4

Author(s):

Shuang Song ◽

Haoran Peng ◽

Qingling Wang ◽

Zhengqi Liu ◽

Xiuping Dong ◽

...

Keyword(s):

Red Algae ◽

Brown Algae ◽

Sea Cucumber ◽

Sulfated Polysaccharide ◽

Sulfated Polysaccharides ◽

Inhibitory Activities

Sulfated polysaccharide from sea cucumber (SCSP), fucoidan from brown algae, and iota-carrageenan from red algae show inhibitory activities against SARS-CoV-2.

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An antithrombin-dependent sulfated polysaccharide isolated from the green alga Caulerpa cupressoides has in vivo anti- and prothrombotic effects

Ciência Rural ◽

10.1590/s0103-84782011000400014 ◽

2011 ◽

Vol 41 (4) ◽

pp. 634-639 ◽

Cited By ~ 21

Author(s):

José Ariévilo Gurgel Rodrigues ◽

Ismael Nilo Lino de Queiroz ◽

Ana Luíza Gomes Quinderé ◽

Bruno Cunha Vairo ◽

Paulo Antônio de Souza Mourão ◽

...

Keyword(s):

Body Weight ◽

Green Algae ◽

Red Algae ◽

Sulfated Polysaccharide ◽

Sulfated Polysaccharides ◽

Antithrombotic Activity ◽

Thrombin Inhibition ◽

Antithrombotic Effects ◽

Mechanism Of Interaction

Red algae sulfated polysaccharides (SPs) have been widely described as anticoagulant and antithrombotic agents; however no description of antithrombotic activity regarding green algae SPs has been reported. Caulerpa cupressoides (Chlorophyta) has three different SPs fractions (SP1, SP2 and SP3). We investigated the effects of SP2 on thrombin activity by antithrombin and in an experimental model of venous thrombosis in rats. The inhibition of thrombin assay was evaluated using antithrombin (AT) in the presence of SP2 and the antithrombotic activity was investigated in rats with thromboplastin as the thrombogenic stimulus. The anticoagulant effects of SP2 are suggested be due to the potentiation of thrombin inhibition by antithrombin (IC50 ~ 10.0µg mL-1) and this mechanism of interaction is different when compared to other studied Caulerpa polysaccharides. SP2 exhibited antithrombotic effects at doses of 1.0 and 2.0mg kg-1 body weight, but at higher doses (>2.0mg kg-1 body weight) this polysaccharide revert the antithrombotic property. No hemorrhagic effect (2.0mg kg-1) was observed. As occurs with red algae SPs, these results indicate that green algae SPs are also capable of exhibiting different in vivo properties.

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- Structure Elucidation and Biological Effects of Carrageenans from Red Algae

Marine Biomaterials ◽

10.1201/b14723-10 ◽

2013 ◽

pp. 84-101

Keyword(s):

Red Algae ◽

Structure Elucidation ◽

Biological Effects

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STRUCTURAL CHARACTERISTICS AND BIOLOGICAL ACTIVITY OF SULFATED POLYSACCHARIDE FROM RED ALGAE BETAPHYCUS GELATINUS

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/58/6a/15598 ◽

2021 ◽

Vol 58 (6A) ◽

pp. 252

Author(s):

Van Thi Thanh Van ◽

Hieu Mai Nhu Vo ◽

Hang Thi Thuy Cao ◽

Trinh Thi Hoai Phan ◽

Duc Mai Tran ◽

...

Keyword(s):

Antioxidant Activity ◽

Biological Activity ◽

Red Algae ◽

Structural Characteristics ◽

Extraction Methods ◽

Sulfated Polysaccharide ◽

Sulfated Polysaccharides ◽

Activity Evaluation ◽

Nmr Methods ◽

Different Levels

In this paper, sulfated polysaccharides were extracted from red algae Betaphycus gelatinus species collected at Ninh Thuan sea by using several extraction methods. The structural characteristics of sulfated polysaccharides was investigated by IR and NMR methods. The results indicated that polysaccharide from Betaphycus gelatinus identified as hybrid carrageenan, mainly composed of β-carrageenan, κ-carrageenan and g-carrageenan. Biological activity evaluation showed that the sulfated polysaccharides possessed antioxidant activity with different levels.

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Ultrastructural Changes in Three Different Mammalian Cells Following Ultraviolet Laser Irradiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100095017 ◽

1972 ◽

Vol 30 ◽

pp. 350-351

Author(s):

K. Shankar Narayan ◽

Kailash C. Gupta ◽

Tohru Okigaki

Keyword(s):

Ultraviolet Light ◽

Mammalian Cells ◽

Biological Effects ◽

Survival Rates ◽

Chinese Hamster ◽

Cell Types ◽

Differential Sensitivity ◽

Ultrastructural Changes ◽

Ultraviolet Laser

The biological effects of short-wave ultraviolet light has generally been described in terms of changes in cell growth or survival rates and production of chromosomal aberrations. Ultrastructural changes following exposure of cells to ultraviolet light, particularly at 265 nm, have not been reported.We have developed a means of irradiating populations of cells grown in vitro to a monochromatic ultraviolet laser beam at a wavelength of 265 nm based on the method of Johnson. The cell types studies were: i) WI-38, a human diploid fibroblast; ii) CMP, a human adenocarcinoma cell line; and iii) Don C-II, a Chinese hamster fibroblast cell strain. The cells were exposed either in situ or in suspension to the ultraviolet laser (UVL) beam. Irradiated cell populations were studied either "immediately" or following growth for 1-8 days after irradiation.Differential sensitivity, as measured by survival rates were observed in the three cell types studied. Pattern of ultrastructural changes were also different in the three cell types.

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Genomic analysis of C-type lectins

Biochemical Society Symposium ◽

10.1042/bss0690059 ◽

2002 ◽

Vol 69 ◽

pp. 59-72 ◽

Cited By ~ 85

Author(s):

Kurt Drickamer ◽

Andrew J. Fadden

Keyword(s):

Biological Effects ◽

Cell Signalling ◽

Genomic Analysis ◽

Binding Activity ◽

Immunoglobulin Superfamily ◽

Carbohydrate Binding ◽

Carbohydrate Recognition ◽

Calcium Dependent ◽

Binding Domains ◽

Carbohydrate Recognition Domains

Many biological effects of complex carbohydrates are mediated by lectins that contain discrete carbohydrate-recognition domains. At least seven structurally distinct families of carbohydrate-recognition domains are found in lectins that are involved in intracellular trafficking, cell adhesion, cell–cell signalling, glycoprotein turnover and innate immunity. Genome-wide analysis of potential carbohydrate-binding domains is now possible. Two classes of intracellular lectins involved in glycoprotein trafficking are present in yeast, model invertebrates and vertebrates, and two other classes are present in vertebrates only. At the cell surface, calcium-dependent (C-type) lectins and galectins are found in model invertebrates and vertebrates, but not in yeast; immunoglobulin superfamily (I-type) lectins are only found in vertebrates. The evolutionary appearance of different classes of sugar-binding protein modules parallels a development towards more complex oligosaccharides that provide increased opportunities for specific recognition phenomena. An overall picture of the lectins present in humans can now be proposed. Based on our knowledge of the structures of several of the C-type carbohydrate-recognition domains, it is possible to suggest ligand-binding activity that may be associated with novel C-type lectin-like domains identified in a systematic screen of the human genome. Further analysis of the sequences of proteins containing these domains can be used as a basis for proposing potential biological functions.

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