Nine nonpregnant, female white-tailed deer (Odocoileus virginianus), 2 to 4 years of age, were used to determine whether body composition can be estimated from deuterium oxide dilution in body water. Venous blood was collected at 0, 20, 30, 40, 50, 60, 80, 120, 240, and 480 min and at 24 and 48 h after deuterium oxide infusion. The deer were then killed and analyzed for dry matter, crude protein, ether extract, and ash. Deuterium oxide dilution, extrapolated to zero time, overestimated analyzed body water by 6%, but the two measures were highly correlated (r2 = 0.85). Incorporation of live weight with estimated body water in the prediction equation increased r2 to 0.95. Ingesta-free body crude protein and ether extract were highly predictable (r2 = 0.92 and 0.96, respectively) from live weight (WT) and estimated total body water (ETBW). The prediction equation for ingesta-free body ether extract was EE = −7.520 + 0.6110(WT) – 0.5417(ETBW), with all measures expressed in kilograms. When ETBW was determined from deuterium oxide dilution in a single 2-h postinfusion blood sample, the prediction equation for ingesta-free body ether extract was EE = −6.306 + 0.6977(WT) – 0.6870(ETBW) (r2 = 0.94).