scholarly journals Separation of high unsaturated fatty acid by use of supercritical gas chromatography.

1989 ◽  
Vol 15 (3) ◽  
pp. 446-450 ◽  
Author(s):  
Masayoshi Kubota ◽  
Harumi Matsuzaki ◽  
Sankichi Takahashi ◽  
Setsuo Inoue
2014 ◽  
Vol 11 (3) ◽  
pp. 1107-1112
Author(s):  
Baghdad Science Journal

The fatty acid composition in the seed and flower of Ligustrun lucidum and olive oil was studied by Gas Chromatography. Results showed that the main components of seed oil were Palmitic (C16:0) 5,893% ,Palmitolic acid (C16:1)0,398%, Steaeic (C18:0)2,911% ,Oleic (C18:1)74,984%,Linoleic (C18:2) 12,959%,and Linolenic (C18:3) 0,997%. The proportion of unsaturated fatty acid was above 89,338%, so the seed oil of L. lucidum ait belonged to unsaturated oil which possessed promising application. The components of flower oil were Palmitic (C16:0) 65,674% ,Palmitolic acid (C16:1)6,516%, Steaeic (C18:0)2,641% ,Oleic (C18:1)14,707%,Linoleic (C18:2) 3,113%,and Linolenic (C18:3) 2,70%. The proportion of unsaturated fatty acid and saturated fatty acid was above 26,406%, 68,315%,respectively so the flower oil of ligustrun lucidum belonged to saturated oil . the main components of olive oil were Palmitic (C16:0) 13,364% ,Palmitolic acid (C16:1)0,834%, Steaeic (C18:0)3,860% ,Oleic (C18:1) 68,668%,Linoleic (C18:2) 12,586%,and Linolenic (C18:3) 0,687%. The proportion of unsaturated fatty acid was above 82,775%, so the olive oil of ligustrun lucidum ait belonged to. These values of seed oil are very similar to that found in the olive oil.


RSC Advances ◽  
2016 ◽  
Vol 6 (27) ◽  
pp. 22436-22446 ◽  
Author(s):  
M. M. Rashed ◽  
H. H. Masjuki ◽  
M. A. Kalam ◽  
Abdullah Alabdulkarem ◽  
H. K. Imdadul ◽  
...  

Moringa oleifera oil, a non-edible biodiesel feedstock with high unsaturated fatty acid content, was used in this study.


2010 ◽  
Vol 1 (6) ◽  
Author(s):  
Sandra Hermanto ◽  
Anna Muawanah ◽  
Prita Wardhani

Penelitian ini bertujuan untuk mengetahui stabilitas dan tingkat kerusakan lemak nabati dan lemakhewani akibat proses pemanasan pada suhu tinggi. Beberapa lemak nabati dan lemak hewani yangdijadikan sampel dalam penelitian ini meliputi minyak goreng curah, minyak goreng kemasan, minyakikan, margarine, lemak babi, lemak sapi, lemak ayam dan minyak zaitun. Masing-masing sampeldipanaskan pada suhu 110oC selama 30 menit, selanjutnya stabilitas dan tingkat kerusakannnyadianalisis dengan mengukur kadar radikal bebas melalui analisis malondialdehid dengan metodekolorimetri dan komposisi asam lemak jenuh (saturated fatty acid), asam lemak tak jenuh tunggal(mono unsaturated fatty acid) serta asam lemak tak jenuh ganda (poly unsaturated fatty acid) denganmenggunakan Gas Chromatography Mass Spectrofotometry (GCMS). Hasil penelitian menunjukkanbahwa kandungan radikal bebas sebagai parameter kerusakan lemak pada masing-masing sampelrelatif berbeda dimana pada minyak ikan dihasilkan radikal bebas sebesar 40 μmol/L, sedangkan padaminyak goreng curah sebesar 25 μmol/L, minyak goreng kemasan 20 μmol/L, margarine 16 μmol/L,minyak zaitun 30 μmol/L, lemak ayam 37 μmol/L, lemak sapi 18 μmol/L dan lemak babi 31 μmol/L.Hasil analisa GCMS menunjukkan bahwa kandungan asam lemak jenuh terbesar diperoleh padasampel lemak sapi (65.53%), sedangkan asam lemak tidak jenuh ganda terbesar diperoleh pada minyakikan sebesar 30.24%. Hal ini mengindikasikan bahwa tingkat kerusakan lemak pada masing-masingsampel sangat dipengaruhi oleh kandungan awal asam lemak tak jenuh ganda yang terdapat padamasing-masing sampel seperti pada minyak ikan dimana komposisi asam lemak tidak jenuh gandarelatif lebih besar dibandingkan dengan yang lain.


2021 ◽  
Vol 12 ◽  
Author(s):  
Jianfeng Liu ◽  
Heng Wei ◽  
Xingzheng Zhang ◽  
Hongli He ◽  
Yunqing Cheng ◽  
...  

Corylus heterophylla (2n = 22) is the most widely distributed, unique, and economically important nut species in China. Chromosome-level genomes of C. avellana, C. heterophylla, and C. mandshurica have been published in 2021, but a satisfactory hazelnut genome database is absent. Northeast China is the main distribution and cultivation area of C. heterophylla, and the mechanism underlying the adaptation of C. heterophylla to extremely low temperature in this area remains unclear. Using single-molecule real-time sequencing and the chromosomal conformational capture (Hi-C) assisted genome assembly strategy, we obtained a high-quality chromosome-scale genome sequence of C. heterophylla, with a total length of 343 Mb and scaffold N50 of 32.88 Mb. A total of 94.72% of the test genes from the assembled genome could be aligned to the Embryophyta_odb9 database. In total, 22,319 protein-coding genes were predicted, and 21,056 (94.34%) were annotated in the assembled genome. A HazelOmics online database (HOD) containing the assembled genome, gene-coding sequences, protein sequences, and various types of annotation information was constructed. This database has a user-friendly and straightforward interface. In total, 439 contracted genes and 3,810 expanded genes were identified through genome evolution analysis, and 17 expanded genes were significantly enriched in the unsaturated fatty acid biosynthesis pathway (ko01040). Transcriptome analysis results showed that FAD (Cor0058010.1), SAD (Cor0141290.1), and KAT (Cor0122500.1) with high expression abundance were upregulated at the ovule maturity stage. We deduced that the expansion of these genes may promote high unsaturated fatty acid content in the kernels and improve the adaptability of C. heterophylla to the cold climate of Northeast China. The reference genome and database will be beneficial for future molecular breeding and gene function studies in this nut species, as well as for evolutionary research on species of the order Fagales.


2019 ◽  
Vol 57 (6) ◽  
pp. 665 ◽  
Author(s):  
Yen Thi Hoang ◽  
Quynh Thi Thu Tran ◽  
Ha Hoang Chu ◽  
Tuyen Thi Do ◽  
Thanh Tat Dang ◽  
...  

Purple nonsulfur bacteria are a group that has so much biotechnological applications, particularly in producing of functional food rich with unsaturated fatty acids. A purple nonsulfur bacterium (named HPB.6) was chosen based on its strong growth, high lipid and synthesis of unsaturated fatty acid (omega 6,7,9). Studying on basic biological characteristics showed that the cells of HPB.6 were observed as ovoid-rod shape, none motility, Gram negative staining. The diameter of single bacterium was about 0.8-1.0 µm. The cells divide by binary fission and had bacteriochlorophyll a (Bchl a). This bacterium grew well on medium with carbon and nitrogen sources such as acetate, succinate, pyruvate, butyrate, glutamate, arginine, leucine, tyrosine, alanine, methionine, threonine, glutamine, yeast extract and NH4Cl. This selected strain grew well on medium with salt concentrations from 1.5 - 6.0% (optimum 3%), pH from 5.0 to 8.0 (optimum at pH 6.5) and could withstand Na2S at 4.0 - 5.2 mM. Based on morphological, physiological properties and 16S rRNA analysis received demonstrated that HPB.6 strain belongs to the species Rhodovulum sulfidophilum.


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