Promoter of Arabidopsis thaliana phosphate transporter gene drives root-specific expression of transgene in rice

AbstractDuring the double fertilization process, pollen tubes deliver two sperm cells to an ovule containing the female gametes. In the pollen tube, the vegetative nucleus and sperm cells move together to the apical region where the vegetative nucleus is thought to play a crucial role in controlling the direction and growth of the pollen tube. Here, we report the generation of pollen tubes in Arabidopsis thaliana whose vegetative nucleus and sperm cells are isolated and sealed by callose plugs in the basal region due to apical transport defects induced by mutations in the WPP domain-interacting tail-anchored proteins (WITs) and sperm cell-specific expression of a dominant mutant of the CALLOSE SYNTHASE 3 protein. Through pollen-tube guidance assays, we show that the physiologically anuclear mutant pollen tubes maintain the ability to grow and enter ovules. Our findings provide insight into the sperm cell delivery mechanism and illustrate the independence of the tip-localized vegetative nucleus from directional growth control of the pollen tube.

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Production of hydroxy fatty acids in the seeds of Arabidopsis thaliana

Biochemical Society Transactions ◽

10.1042/bst0280947 ◽

2000 ◽

Vol 28 (6) ◽

pp. 947-950 ◽

Cited By ~ 12

Author(s):

M. Smith ◽

H. Moon ◽

L. Kunst

Keyword(s):

Fatty Acids ◽

Arabidopsis Thaliana ◽

Seed Oil ◽

Desaturase Activity ◽

Hydroxy Fatty Acids ◽

Specific Expression ◽

Oleate Desaturase ◽

Arabidopsis Mutant ◽

Mutant Lines ◽

Seed Specific Expression

Seed-specific expression in Arabidopsis thaliana of oleate hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of hydroxy fatty acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n–-3 desaturase (FAD3) and the FAE1 condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain hydroxy fatty acids, respectively. In Arabidopsis plants with an active ER Δ12-oleate desaturase the presence of hydroxy fatty acids corresponded to an increase in the levels of 18:1 and a decrease in 18:2 levels. Expression in yeast indicates that the castor hydroxylase also has a low level of desaturase activity.

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Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Journal of Visualized Experiments ◽

10.3791/1925 ◽

2010 ◽

Author(s):

Sankalpi N. Warnasooriya ◽

Beronda L. Montgomery

Keyword(s):

Arabidopsis Thaliana ◽

Expression Profiling ◽

Cell Type ◽

Specific Expression ◽

Organ Specific ◽

Cell Type Specific Expression ◽

Cell Type Specific

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Sucrose Transporter Gene AtSUC4 Responds to Drought Stress by Regulating the Sucrose Distribution and Metabolism in Arabidopsis thaliana

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.765-767.2971 ◽

2013 ◽

Vol 765-767 ◽

pp. 2971-2975 ◽

Cited By ~ 4

Author(s):

Xue Gong ◽

Ming Li Liu ◽

Li Jun Zhang ◽

Wei Liu ◽

Che Wang

Keyword(s):

Arabidopsis Thaliana ◽

Drought Stress ◽

Plant Stress ◽

Homozygous Mutation ◽

Transporter Gene ◽

Wild Type ◽

Sucrose Transporters ◽

Whole Plant ◽

Plant Stress Tolerance ◽

Plant Level

Sucrose transporters (SUCs or SUTs) are considered as the important carriers and responsible for the loading, unloading and distribution of sucrose, but at present there is no report that SUCs are involved in sucrose distribution and metabolism under drought stress at the whole-plant level. AtSUC4, as the unique member of SUT4-clade inArabidopsis thaliana, may be important for plant stress tolerance. Here, by analyzing two homozygous mutation lines ofAtSUC4(Atsuc4-1andAtsuc4-2), we found drought stress induced higher sucrose, lower fructose and glucose contents in shoots, and lower sucrose, higher fructose and glucose contents in roots of these mutants compared with the wild-type (WT), leading to an imbalance of sucrose distribution, fructose and glucose (sucrose metabolites) accumulation changes at the whole-plant level. Thus we believe thatAtSUC4regulates sucrose distribution and metabolism in response to drought stress.

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