Production of hydroxy fatty acids in the seeds of Arabidopsis thaliana

2000 ◽  
Vol 28 (6) ◽  
pp. 947-950 ◽  
Author(s):  
M. Smith ◽  
H. Moon ◽  
L. Kunst
Keyword(s):  
Fatty Acids ◽  
Arabidopsis Thaliana ◽  
Seed Oil ◽  
Desaturase Activity ◽  
Hydroxy Fatty Acids ◽  
Specific Expression ◽  
Oleate Desaturase ◽  
Arabidopsis Mutant ◽  
Mutant Lines ◽  
Seed Specific Expression

Seed-specific expression in Arabidopsis thaliana of oleate hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of hydroxy fatty acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n–-3 desaturase (FAD3) and the FAE1 condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain hydroxy fatty acids, respectively. In Arabidopsis plants with an active ER Δ12-oleate desaturase the presence of hydroxy fatty acids corresponded to an increase in the levels of 18:1 and a decrease in 18:2 levels. Expression in yeast indicates that the castor hydroxylase also has a low level of desaturase activity.

The role of diacylglycerol acyltransferase-1 and phospholipid:diacylglycerol acyltransferase-1 and -2 in the incorporation of hydroxy fatty acids into triacylglycerol in Arabidopsis thaliana expressing a castor bean oleate 12-hydroxylase geneThis paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.

Botany ◽  
2009 ◽  
Vol 87 (6) ◽  
pp. 552-560 ◽  
Author(s):  
Melanie Dauk ◽  
Patricia Lam ◽  
Mark A. Smith
Keyword(s):  
Fatty Acids ◽  
Neutral Lipids ◽  
National Research Council ◽  
Plant Biotechnology ◽  
Hydroxy Fatty Acids ◽  
Acyltransferase Activity ◽  
Unusual Fatty Acids ◽  
Arabidopsis Mutant ◽  
Mutant Lines

Expression of oleate 12-hydroxylase genes in Arabidopsis results in the accumulation of hydroxy fatty acids in seed triacylglycerol (TAG). The pathways by which these unusual fatty acids become incorporated into TAG are not well understood. We expressed a fatty acid hydroxylase cDNA in Arabidopsis mutant lines to assess the role of three enzymes implicated in TAG assembly in this species. Plants deficient in the expression of phospholipid:diacylglycerol acyltransferase-1 or -2 accumulated hydroxy fatty acids and showed no differences to equivalent transformed wild-type plants. Plants lacking diacylglylcerol acyltransferase activity were also able to accumulate hydroxy fatty acids in seed neutral lipids. Triacylglycerol species containing one and two hydroxy fatty acids were abundant, and small amounts of trihydroxy-TAG were detected. These results indicate that individually, the three enzymes do not play a major role in the incorporation of hydroxy fatty acids into TAG.

Production of value-added hydroxy fatty acids from Korean pine seed oil by Pseudomonas aeruginosa

2008 ◽  
Vol 136 ◽  
pp. S398-S399
Author(s):  
Hak-Ryul Kim ◽  
Deuk-Soo Kim ◽  
Min-Jung Suh ◽  
Jae-Han Bae ◽  
Jong-Sang Kim ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Pseudomonas Aeruginosa ◽  
Seed Oil ◽  
Value Added ◽  
Hydroxy Fatty Acids ◽  
Korean Pine ◽  
Pine Seed Oil ◽  
Korean Pine Seed Oil

Early accumulation of non-enzymatically synthesised oxylipins in Arabidopsis thaliana after infection with Pseudomonas syringae

2007 ◽  
Vol 34 (1) ◽  
pp. 65 ◽  
Author(s):  
Christoph Grun ◽  
Susanne Berger ◽  
Daniel Matthes ◽  
Martin J. Mueller
Keyword(s):  
Reactive Oxygen Species ◽  
Fatty Acids ◽  
Arabidopsis Thaliana ◽  
Jasmonic Acid ◽  
Pseudomonas Syringae ◽  
Virulent Strain ◽  
Reactive Oxygen ◽  
Hydroxy Fatty Acids ◽  
Avirulent Strain ◽  
Oxygen Species

The formation of non-enzymatic oxylipins is catalysed by reactive oxygen species. Reactive oxygen species are produced in response to pathogen attack. In this study, the accumulation of non-enzymatically formed hydroxy fatty acids and F1-phytoprostanes in leaves of Arabidopsis thaliana (L.) Heyhn upon infection with Pseudomonas syringae was investigated and compared with the accumulation of the enzymatically formed oxylipins jasmonic acid and 12-oxo-phytodienoic acid. Levels of all oxylipins increased after infection with a virulent and with an avirulent strain of P. syringae. Inoculation of the avirulent strain resulted in a biphasic accumulation with a first maximum around 5 h which was missing after inoculation of the virulent strain. Levels of free and esterified hydroxy fatty acids and F1-phytoprostanes increased after pathogen treatment; however, esterified compounds were 30 times more abundant than free oxylipins. The increase of the free compounds occurred later than the increase of the esterified compounds suggesting that non-enzymatic lipid oxidation occurs predominantly in membranes from which oxidised lipids can be released.

Sign in / Sign up

Export Citation Format

Share Document