Research on Antibacterial Activities of Amikacin Sulfate Combined with the Ingredients of Coptis chinensis against Multiple Drug Resistance Klebsiella pneumoniae in vitro

2021 ◽  
Vol 10 (06) ◽  
pp. 315-320
Author(s):  
登钊 姜
Keyword(s):  
Drug Resistance ◽  
Klebsiella Pneumoniae ◽  
Multiple Drug Resistance ◽  
Antibacterial Activities ◽  
Multiple Drug ◽  
Coptis Chinensis ◽  
Amikacin Sulfate

scholarly journals Dramatic Activation of Antibiotic Production in Streptomyces coelicolor by Cumulative Drug Resistance Mutations

2008 ◽  
Vol 74 (9) ◽  
pp. 2834-2840 ◽  
Author(s):  
Guojun Wang ◽  
Takeshi Hosaka ◽  
Kozo Ochi
Keyword(s):  
Drug Resistance ◽  
Multiple Drug Resistance ◽  
Antibiotic Production ◽  
Streptomyces Coelicolor ◽  
Strain Improvement ◽  
Multiple Drug ◽  
Resistance Mutations ◽  
Drug Resistance Mutations ◽  
Order Of Magnitude

ABSTRACT We recently described a new method to activate antibiotic production in bacteria by introducing a mutation conferring resistance to a drug such as streptomycin, rifampin, paromomycin, or gentamicin. This method, however, enhanced antibiotic production by only up to an order of magnitude. Working with Streptomyces coelicolor A3(2), we established a method for the dramatic activation of antibiotic production by the sequential introduction of multiple drug resistance mutations. Septuple and octuple mutants, C7 and C8, thus obtained by screening for resistance to seven or eight drugs, produced huge amounts (1.63 g/liter) of the polyketide antibiotic actinorhodin, 180-fold higher than the level produced by the wild type. This dramatic overproduction was due to the acquisition of mutant ribosomes, with aberrant protein and ppGpp synthesis activity, as demonstrated by in vitro protein synthesis assays and by the abolition of antibiotic overproduction with relA disruption. This new approach, called “ribosome engineering,” requires less time, cost, and labor than other methods and may be widely utilized for bacterial strain improvement.

scholarly journals Klebsiella Pneumoniae in Septicemic Neonates with Special Reference to Extended Spectrum β-lactamase, AmpC, Metallo β-lactamase Production and Multiple Drug Resistance in Tertiary Care Hospital

2015 ◽  
Vol 7 (01) ◽  
pp. 032-037 ◽  
Author(s):  
Shivali V Gajul ◽  
Shivajirao T Mohite ◽  
Smita S Mangalgi ◽  
Sanjay M Wavare ◽  
Satish V Kakade
Keyword(s):  
Drug Resistance ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Susceptibility ◽  
Multiple Drug Resistance ◽  
Confirmatory Test ◽  
Diffusion Method ◽  
Multiple Drug ◽  
Neonatal Septicemia ◽  
Extended Spectrum ◽  
Disk Method

ABSTRACT Background: β-lactamases viz., extended spectrum β-lactamase (ESBL), AmpC, and metallo β-lactamase (MBL) production in Klebsiella pneumoniae has led to a serious concern about septicemic neonates in Neonatal Intensive Care Units due to high resistance against commonly used antimicrobials Purpose:To study the prevalence of ESBL, AmpC, and MBL production in K. pneumoniae isolates in neonatal septicemia, to check antimicrobial susceptibility to various drugs including tigecycline; and to assess burden of multiple drug resistance (MDR). Materials and Methods: Total 24 clinical isolates of K. pneumoniae isolated from 318 blood samples of suspected cases of neonatal septicemia were studied. Isolates were screened for ESBL, AmpC, and MBL production by Clinical and Laboratory Standards Institute (CLSI) disk method, AmpC cefoxitin screen, and imipenem, meropenem, ceftazidime disk screen respectively; and confirmation was done by CLSI phenotypic disk confirmatory test, AmpC sterile disk method, and imipenem ethylenediamine tetracetic acid double disk synergy test respectively. Antimicrobial susceptibility was determined by Kirby-Bauer's disk diffusion method. Efficacy of tigecycline was evaluated using United States Food and Drug Administration guidelines. Results: Of the 24 K. pneumoniae isolates, co-production of AmpC + MBL was found in more number of isolates (67%) (P < 0.0001) compared to single enzyme production (ESBL and MBL 8% both, AmpC 12.5%). Rate of resistance for penicillins and cephalosporins was highest. Susceptibility was more for imipenem, co-trimoxazole, and meropenem. Nonsusceptibility to tigecycline was low (21%). A total of 23 (96%) isolates were MDR. Conclusions: Routine detection of ESBL, AmpC, and MBL is required in laboratories. Carbapenems should be kept as a last resort drugs. Trend of tigecycline susceptibility has been noted in the study. Continued monitoring of susceptibility pattern is necessary to detect true burden of resistance for proper management.

scholarly journals Multidrug Therapy and Evolution of Antibiotic Resistance: When Order Matters

2012 ◽  
Vol 78 (17) ◽  
pp. 6137-6142 ◽  
Author(s):  
Gabriel G. Perron ◽  
Sergey Kryazhimskiy ◽  
Daniel P. Rice ◽  
Angus Buckling
Keyword(s):  
Drug Resistance ◽  
Multidrug Resistance ◽  
Pathogenic Bacteria ◽  
Health Workers ◽  
Multiple Drug Resistance ◽  
Sequential Therapy ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Multidrug Therapy

ABSTRACTThe evolution of drug resistance among pathogenic bacteria has led public health workers to rely increasingly on multidrug therapy to treat infections. Here, we compare the efficacy of combination therapy (i.e., using two antibiotics simultaneously) and sequential therapy (i.e., switching two antibiotics) in minimizing the evolution of multidrug resistance. Usingin vitroexperiments, we show that the sequential use of two antibiotics againstPseudomonas aeruginosacan slow down the evolution of multiple-drug resistance when the two antibiotics are used in a specific order. A simple population dynamics model reveals that using an antibiotic associated with high costs of resistance first minimizes the chance of multidrug resistance evolution during sequential therapy under limited mutation supply rate. As well as presenting a novel approach to multidrug therapy, this work shows that costs of resistance not only influences the persistence of antibiotic-resistant bacteria but also plays an important role in the emergence of resistance.

scholarly journals Interactions between the gene products of pma1 encoding plasma membrane H(+)-ATPase, and pdr1 controlling multiple drug resistance in Saccharomyces cerevisiae.

1993 ◽  
Vol 40 (4) ◽  
pp. 487-496 ◽  
Author(s):  
S Ułaszewski
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Plasma Membrane ◽  
Atpase Activity ◽  
Multiple Drug Resistance ◽  
Multidrug Resistant ◽  
Multiple Drug ◽  
Gene Products ◽  
In Trans

In Saccharomyces cerevisiae, the pma1 mutations controlling the vanadate resistance of the H(+)-ATPase activity from the plasma membrane, map on chromosome VII in the vicinity of pdr1 mutations controlling multiple drug resistance. However, the pma1-1 mutants exhibit a genotype and a multidrug resistant phenotype quite different from those obtained for pdr1 mutants. Quantitative modifications of cycloheximide and N,N'-(p-xylylidene)-bis-aminoguanidine-2HCl resistance are observed in diploids containing the pma1 and pdr1 genes in trans configuration. Each of the pdr1 mutations interacts with pma1 as shown by a decrease in the ATPase activity in pdr1/pma1 diploids. The in vitro resistance of ATPase activity to vanadate is totally or partially suppressed in pdr1 mutants in haploid double mutants. These results suggest that the expression of PMA1 might be controlled by the PDR1 gene product.

scholarly journals Plasmid Analysis and Curing of Multidrug Resistant of Helicobacter pylori Isolated from Ulcer Patients in Ondo State, Nigeria

2019 ◽  
pp. 1-8
Author(s):  
A. K. Onifade ◽  
M. A. Bakare
Keyword(s):  
Drug Resistance ◽  
Helicobacter Pylori ◽  
Multiple Drug Resistance ◽  
Multidrug Resistant ◽  
Plasmid Profile ◽  
Multiple Drug ◽  
In Vitro Susceptibility ◽  
Ondo State ◽  
H Pylori

Plasmid profile of multidrug resistant Helicobacter pylori isolated from ulcer patients in Ondo State, Nigeria was investigated. It was observed that the H. pylori isolated from ulcer patients in the course of the research were resistance to certain antibiotics. Consequently on the emergence of multiple drug resistance exhibited by the H. pylori, a total of 9 different antibiotics were tested against the H. pylori isolated from various locations to determine their in-vitro susceptibility pattern. The findings of the in-vitro antibiotics susceptibility testing are quite astonishing due to a high incidence of multiple drug resistance. Majority of the H. pylori showed high level of resistance against some specific number of antibiotics.  The multidrug resistance observed in this study seems to be plasmid mediated. Moreover frequent use of over-the-counter antibiotics and abuse of non steroidal anti inflammatory drugs (NSAIDs) administration and abuse of drugs of sub – standard chemical quality has been identified as a major problem. The results from this investigative study have shown that resistance in H. pylori was plasmid based.

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