scholarly journals Μέθοδος εμπλουτισμού σπέρματος κριού σε ζωηρά κινούμενα σπερματοζωάρια

2018 ◽  
Vol 61 (2) ◽  
pp. 120
Author(s):  
S. G. PIPERELIS (Σ.Γ. ΠΙΠΕΡΕΛΗΣ) ◽  
D. Vafiadis (Δ. ΒΑΦΕΙΑΔΗΣ) ◽  
C. Alexopoulos (Κ. ΑΛΕΞΟΠΟΥΛΟΣ)
Keyword(s):  
Glass Beads ◽  
Initial Value ◽  
Filtration Time ◽  
Filtration Method ◽  
Significant Difference ◽  
Further Development ◽  
Time Density ◽  
Production Facilities

Semen availability in ram semen production facilities is of great importance and a method that would increase viability in low viability ejaculates could be useful. In this study the possibility of a glass beads filtration method estimating the beads total surface provided (TSP) for adhesion of spermatozoa is evaluated. Initially, two different TSP (102cm2,154cm2) achieved by various sizes of beads were tested and no significant difference in viability improvement appeared for the same TSP by different beads (p<0.05). An optimization test followed in which three different funnels were evaluated using standard TSP (154cm2). The only parameter improved was filtrate volume, while filtration time, density and viability improvement remained the same (p<0.05).Finally, different TSP were evaluated and significant differences in viability improvement (p<0.01) and filtrate volume (p<0.05)for 102cm2 appeared. Increase in initial value 25% for viability and ejaculate recovery 65% all in time less than 6 minutes stronglysuggest that through further development this method can be used in ram semen production facilities.

An Assessment of Red Cell Deformability Using a Simple Filtration Method

1979 ◽  
Author(s):  
M Drummond ◽  
G Lowe ◽  
J Belch ◽  
C Forbes ◽  
J Barbenel
Keyword(s):  
Cell Count ◽  
Shear Viscosity ◽  
White Cell Count ◽  
White Cell ◽  
Red Cell ◽  
Cell Deformability ◽  
Red Cell Deformability ◽  
Simple Method ◽  
Filtration Method ◽  
Significant Difference

We investigated the reproducibility and validity of a simple method of measuring red cell deformability (filtration of whole blood through 5 µ sieves) and its relationship to haematocrit, blood viscosity, fibrinogen, white cell count, sex and smoking. The mean coefficient of variation in normals was 3. 7%. Tanned red cells showed marked loss of deformability. Blood filtration rate correlated with haematocrit (r = 0. 99 on dilution of samples, r = 0. 7 in 120 normals and patients). After correction for haematocrit, deformability correlated with high shear viscosity, but not low shear viscosity, fibrinogen or white cell count. In 60 normals there was no significant difference between males and females, or smokers and non-smokers, but in 11 smokers there was an acute fall in deformability after smoking 3 cigarettes (p<0. 05). Reduced deformability was found in acute myocardial infarction (n = 15, p<0. 01) and chronic peripheral arterial disease (n = 15, p<0. 01). The technique is reproducible, detects rigid cells and appears useful in the study of vascular disease.

Influence of Impregnation-Vacuum Filtration Conditions on Ni Dispersion and Activity of Ni-Cordierite for Hydrogenation

2013 ◽  
Vol 781-784 ◽  
pp. 312-319
Author(s):  
Shao Wen Liu ◽  
Zhuo Long Guan ◽  
Lei Qing ◽  
Jiu Ling Chen ◽  
Hai Nan Wang ◽  
...  
Keyword(s):  
Solution Concentration ◽  
Catalytic Performance ◽  
Filtration Time ◽  
Filtration Method ◽  
Vacuum Filtration ◽  
Structured Catalysts ◽  
Preparation Conditions ◽  
Vacuum Degree ◽  
Close Relationship ◽  
Impregnation Time

The influence of the preparation conditions of the impregnation-vacuum filtration method was investigated systematically on the Ni dispersion and the activity of Ni-cordierite structured catalysts in hydrogenation of m-dinitrobenzene to m-phenylenediamine. H2-TPD measurement showed that the Ni dispersion has close relationship with the impregnation solution concentration of nickel nitrate, the impregnation time, the vacuum degree, the vacuum filtration time and the calcination temperature. The hydrogenation activity test and nitrogen physisorption investigation showed that the catalytic performance of Ni-cordierite is dependent upon the Ni dispersion and the chemisorption mode of m-dinitrobenzene on Ni particles.

scholarly journals Evaluation of Urine Reagent Strip as a Tool for Routine Diagnosis of Maternal Urogenital Schistosomiasis at Antenatal Clinic Visit in Munyenge, South West Region, Cameroon

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Godlove Bunda Wepnje ◽  
Judith Kuoh Anchang-Kimbi ◽  
Leopold Gustave Lehman ◽  
Helen Kuokuo Kimbi
Keyword(s):  
Health Centre ◽  
Antenatal Clinic ◽  
Clinic Visit ◽  
Functional Health ◽  
Egg Load ◽  
Sample Collection ◽  
Urogenital Schistosomiasis ◽  
Filtration Method ◽  
Predictive Values ◽  
Significant Difference

Urine reagent strip used in detecting microhaematuria has been recommended in pregnancy for diagnosis of urogenital schistosomiasis (UGS) during routine antenatal care (ANC). This study evaluated its sensitivity, specificity, and predictive values in the diagnosis of maternal UGS using filtration method as a reference test. We also assessed the variation in its performance in the diagnosis of UGS using multiple-sample collection. A total of 93 pregnant women reporting for first ANC clinic visit at any of the three functional health care centres (Munyenge Integrated Health Centre, Banga Annex Health Centre, and Trans African Health Centre) were enrolled and followed up for three consecutive monthly visits. Urine samples were observed microscopically for S. haematobium egg using urine filtration and screened for microhaematuria and proteinuria using urine reagent strips. Twenty-two (23.7%) out of the 93 women were diagnosed for UGS, all of whom showed S. haematobium egg excretion during all three visits. There was a significant difference (p<0.001) between the prevalence of S. haematobium infection and the prevalence of microhaematuria. The intensity of infection was significantly higher in microhaematuria-positive women compared with microhaematuria-negative cases. Sensitivity of reagent strip ranged from 54.5 to 59.1%, while specificity was above 98.0% (range: 98.6–100%). The measure of agreement between urine filtration and reagent strip method was substantial (0.61–0.8) irrespective of different sampling periods. Urine reagent strip is a moderately sensitive method in the detection of UGS and will most likely identify women with high egg load burden. Proper diagnosis of schistosomiasis during pregnancy is recommended.

Development of prepubertal goat oocytes after their in vitro maturation and chemical activation

Zygote ◽  
2020 ◽  
Vol 28 (6) ◽  
pp. 447-452
Author(s):  
Seungbum Hong ◽  
Binoy S. Vettical ◽  
Nisar Ahmad Wani
Keyword(s):  
Culture Media ◽  
Chemical Activation ◽  
Control Treatment ◽  
Developmental Potential ◽  
Embryo Culture Medium ◽  
Significant Difference ◽  
Maturation Rate ◽  
Further Development ◽  
Oviductal Fluid

SummaryExperiments were conducted to study in vitro maturation of prepubertal goat oocytes and their developmental potential after chemical activation. In Experiment 1, cumulus–oocytes complexes collected from the ovaries of prepubertal goats slaughtered at a local abattoir were matured in vitro in TCM-199-based medium supplemented with 10 µg/ml luteinizing hormone (LH) (treatment 1) or 10 µg/ml LH + 0.1 mM l-cysteine (treatment 2). In Experiment 2, mature oocytes were activated with either 5 µM ionomycin or 7% ethanol. After 18 h, some oocytes were randomly fixed and stained to evaluate their chromatin status, while others were cultured in embryo culture medium to study their further development. In Experiment 3, oocytes activated with 5 µM ionomycin were cultured for 7 days in one of the four different culture media [Charles Rosenkrans medium (CR-1), TCM-199, potassium simplex optimization medium (KSOM) and synthetic oviductal fluid (SOF)] to study their developmental potential. The maturation rate in control, treatment 1, and treatment 2 media did not differ from each other (P > 0.05). However, the lowest degeneration of oocytes was observed in treatment 3 (P < 0.05) when compared with the other two groups. The proportion of activated oocytes was higher, while non-activated oocytes were lower in ionomycin group when compared with the group activated with ethanol (P < 0.05). The proportions of oocytes cleaved were 65.7, 56.8, 61.0 and 54.4% in CR-1, TCM-199, KSOM and SOF medium, respectively, with no significant difference. However, further development of cleaved oocytes was better in KSOM followed by SOF.

Cryopreservation of incomplete compacted morulae and preliminary biopsy of excluded fragments

Zygote ◽  
2019 ◽  
Vol 27 (6) ◽  
pp. 386-391
Author(s):  
Maryna Petrushko ◽  
Taisiia Yurchuk ◽  
Volodymyr Piniaiev ◽  
Natalia Buderatska
Keyword(s):  
Survival Rate ◽  
Formation Rate ◽  
Uterine Cavity ◽  
Embryo Cryopreservation ◽  
Blastocyst Formation ◽  
Significant Difference ◽  
Morula Stage ◽  
Grade 3 ◽  
Further Development

SummaryThe complexity of predicting embryo development potential at the cleavage stages and the emergence of epigenetic risks during prolonged in vitro culture of pre-implantation embryos made it more advantageous to transfer embryos at the morula stage to the uterine cavity. The criteria for estimating embryos at this stage that allow prediction of cryopreservation outcomes have been poorly described. All day 4 embryos (n = 224) were graded 1, 2, 3, 4 or 5 according to blastomere compaction degree (BCD = 100, 75, 50, 25 or 0%, respectively) and the survival and blastocyst formation rate of these morulae were studied after cryopreservation. An inverse dependence was found between survival rate and BCD. Excluded fragments were characterized by low osmotic reaction during exposure to cryoprotective medium and, after freeze-thawing, they were destroyed. As damaged necrotic areas of the embryo can affect their further development rate we proposed blastomeres and biopsy fragments of incomplete compacted morula be removed before embryo cryopreservation. This step led to significant increase in the post-thawing survival rate up to 93.1 ± 4.1%, 75 ± 8.8% and blastocyst formation rate up to 85.2 ± 10.4%, 59.4 ± 5.2% in grade 2 and grade 3 embryos, respectively. There was no significant difference in grade 4 embryos. Therefore the removal of blastomeres and biopsy fragments in incomplete compacted morulae can improve cryopreservation outcomes of grade 2 and grade 3 embryos with BCD.

Preparation of glass standards for the use in X-ray microanalysis

1972 ◽  
Vol 38 (297) ◽  
pp. 614-617 ◽  
Author(s):  
J. A. T. Smellie
Keyword(s):  
Statistical Analysis ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Glass Beads ◽  
Fusion Process ◽  
X Ray ◽  
Natural Minerals ◽  
Significant Difference

SummaryHomogeneous glasses for the use as standards in electron-probe microanalysis were prepared as an alternative to natural minerals, oxides, and pure minerals. Homogeneity was achieved by careful grinding, mixing, and fusion of powders of known composition. Fusion was carried out under vacuum using a molybdenum boat. This method avoided bubbles and contamination and made it possible to achieve temperatures high enough for final mixing to occur during the fusion process. Nine glass beads each containing six to seven oxides were subjected to careful micro-probe examination. A statistical analysis of the results showed that for each element there was no significant difference in composition between the spots measured. As the analysing spot was of diameter 0·8 µ, this showed that the glasses were homogenous on a sub-micron scale.

scholarly journals SAT-040 Changes in Metabolic Parameters After Administration of Novel Oral Androgens with Progestational Activity for 28 Days

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Fiona Yuen ◽  
Arthi Thirumalai ◽  
Ronald S Swerdloff ◽  
Peter Y Liu ◽  
Youngju Pak ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Fasting Glucose ◽  
Hormonal Contraception ◽  
Secondary Analysis ◽  
Metabolic Parameters ◽  
Metabolic Effects ◽  
Double Blind ◽  
Significant Difference ◽  
Post Hoc ◽  
Further Development

Abstract Background: While the metabolic effects of testosterone have been well studied, the effects of co-administration of an androgen and progestin are less established. Two novel compounds being investigated for male hormonal contraception, dimethandrolone undecanoate (DMAU) and 11β-methyl-19-nortestosterone dodecylcarbonate (11β-MNTDC), have both androgenic and progestational activity. Aim: Characterize the effects of DMAU and 11β-MNTDC on metabolic parameters including weight, lipid parameters, insulin resistance, and adiponectin. Methods: Two randomized, double-blind, placebo-controlled studies in healthy men were previously performed to assess the safety and tolerability of DMAU and 11β-MNTDC taken orally for 28 days. Insulin and adiponectin assays were performed on a subset of banked samples. Changes in weight, LDL-C, HDL-C, fasting glucose, HOMA-IR, and adiponectin were assessed. Two way ANOVA with post hoc Tukey HSD was performed to assess for dosage (0, 200, or 400mg) and drug (DMAU or 11β-MNTDC) effects. Results: A total of 85 subjects were included in this secondary analysis. There was a statistically significant decrease in HDL-C (mean change -11 and -15 mg/dL) and increase in weight (3 and 2 kg) and LDL-C (18 and 23 mg/dL) in the DMAU and 11β-MNTDC 400mg groups respectively. There was no significant difference between the 200 and 400 mg groups nor differences between the two androgens. There were no statistically significant changes in fasting glucose, adiponectin or HOMA-IR. Conclusion: There were mild changes in weight, HDL-C, and LDL-C after 28 days of DMAU and 11β-MNTDC without significant changes in markers of insulin resistance or differences between the two compounds. Changes in metabolic parameters should be monitored and considered during further development of compounds for male hormonal contraception.

scholarly journals Fluid flow evaluation of coronal microleakage intraorifice barrier materials in endodontically treated teeth

2013 ◽  
Vol 07 (03) ◽  
pp. 359-362 ◽  
Author(s):  
H. Melike Bayram ◽  
Berkan Çelikten ◽  
Emre Bayram ◽  
Alperen Bozkurt
Keyword(s):  
Statistical Analysis ◽  
Negative Control ◽  
Control Group ◽  
Barrier Material ◽  
Endodontically Treated Teeth ◽  
Fluid Filtration ◽  
Filtration Method ◽  
Barrier Materials ◽  
Nail Polish ◽  
Significant Difference

ABSTRACT Objective: The objective of this study was to compare the coronal microleakage intraorifice barrier materials, called CoroSeal (CS), fissur sealant (FS), flowable composite FC, and policarboksilate cement (PC), by using the computerized fluid filtration method. Materials and Methods: Fifty freshly extracted, single-canal human maxillary central teeth were used in this study. The teeth were decoronated to a standardized root length of 15 mm. After preparation and irrigation, all the teeth were obturated with gutta-percha and AH-Plus. In all teeth, the coronal 2 mm of root filling was removed and replaced with one of the intraorifice barriers. According to intraorifice barriers, teeth were divided randomly into 4 experimental groups (n = 10) and 2 control groups (n = 5). Group 1: CS; Group 2: FS; Group 3: FC; and Group 4: PC. Positive Control Group: No barrier material was used. Negative Control Group: Roots were completely coated with the nail polish, including the orifice. Leakage was evaluated by using a computerized fluid filtration model. Differences in fluid filtration among groups were subjected to statistical analysis using the Kruskal-Wallis Test and multiple comparisons test. Results: A value of P < 0.05 was statistically significant. Statistical analysis has indicated that the CS leaked significantly less than other groups (P < 0.05). There was a significant difference between FS and PC (P < 0.05), in contrast there was no significant difference between FS and FC (P > 0.05). Conclusions: Using the CS material as an intraorrifice barrier material reduced amount of microleakage as compared with FS, FC, and PC.

Intra articular methylprednisolone acetate injection efficacy in the treatment of primary knee osteoarthritis at Hue Central Hospital and Hospital of Hue University of Medicine and Pharmacy

2019 ◽  
pp. 48-54
Author(s):  
Minh Trai Pham ◽  
Hoang Thanh Van Nguyen
Keyword(s):  
Knee Osteoarthritis ◽  
Clinical Symptoms ◽  
Joint Fluid ◽  
Injection Pain ◽  
Control Group ◽  
Membrane Thickness ◽  
Study Group ◽  
Methylprednisolone Acetate ◽  
Initial Value ◽  
Significant Difference

Introduction/Background: Osteoarthritis is still an incurable disease, the current treatment is to reduce clinical symptoms. The intra articular glucocorticoid injection in knee osteoarthritis makes the inflammatory process self-limiting and improves pain symptom. Objectives: To evaluate the efficacy and safety of intra articular methylprednisolone acetate injection in treatment of primary knee osteoarthritis. Materials and Methods: Prospective descriptive study of 88 knee joints (34 joints in study group, 54 joints in control group) was diagnosed with primary osteoarthritis according to the American College of Rheumatology (ACR) classification criteria and in Kellgen & Lawrence grade II, III. The patient was assessed about clinical features, subclinical features and the VAS score, Lequesne index at the initial of the study and 7 days later. Results: The analgesic effect was higher in the group of intra articular methylprednisolon injection with statistically significant difference (∆VAS of the study group and the control group were 38.65 ± 9.25; 25.11 ± 9.26, respectively, with p < 0.05). The improving of knee function was higher in the group of intra articular methylprednisolon injection with statistically significant difference (∆Lequesne of the study group and the control group were 7.00 (5.00 – 8.00); 4.00 (3.00 – 5.00), respectively, with p < 0.05). The incidence of complications in the study group was 1/34 (2.94%) with post – injection pain. The improvement of VAS is better in patient with severe pain than those with moderate pain (p < 0.05) and correlated to the initial value of Lequesne (r2 = 0,162, respectively, with p < 0.05). The improvement of Lequesne correlated to the joint fluid thickness in ultrasound (r2 = 0.102, p < 0.05). There is no relation between efficacy of treatment and site of knee joint, radiology stage and synovial membrane thickness (p > 0.05). Conclusions: The intra articular methylprednisolone acetate injection in treatment of primary knee osteoarthritis is a safe treatment and has significant, clearly effect in pain relief and physical function improving after 7 days. The improvement of VAS is better in the patient with higher initial value of VAS, Lequesne and the improvement of Lequesne is better in the patients with more synovial thickness in ultrasound. Key words: Knee osteoarthritis, intraarticular injection, methylprednisolon acetate

Detection of Campylobacter jejuni from fresh produce: comparison of culture- and PCR-based techniques, and metagenomic approach for analyses of the microbiome before and after enrichment

2021 ◽  
Author(s):  
Jung-Whan Chon ◽  
Ji Young Jung ◽  
Youngbeom Ahn ◽  
Dongryeoul Bae ◽  
Saeed Khan ◽  
...  
Keyword(s):  
Real Time ◽  
Campylobacter Jejuni ◽  
Membrane Filtration ◽  
Detection Efficiency ◽  
Fresh Produce ◽  
Culture Method ◽  
Detection Methods ◽  
Filtration Method ◽  
Significant Difference ◽  
Alfalfa Sprouts

In this study, we compared the efficiency of culture-based methods with or without membrane filtration, real-time PCR and digital droplet PCR (ddPCR) for the detection of Campylobacter in fresh produce. Alfalfa sprouts, clover sprouts, coleslaw, and lettuce salad spiked with Campylobacter jejuni ( C. jejuni ) were enriched in Bolton broth for 48 h and enrichment cultures were either directly inoculated onto modified charcoal-cefoperazone-deoxycholate agar or applied on membrane filters placed on the surface of plating media. In parallel, 2 mL Bolton broth cultures were taken to extract DNA for real-time and ddPCR assays and bacterial community analysis. There was no significant difference ( p &gt; 0.05) in the detection efficiency of positive Campylobacter isolates from coleslaw and lettuce salad using four detection methods. However, for sprout samples, the detection efficiency of the culture method was significantly ( p &lt; 0.05) lower than those of the two PCR assays and the filtration method. The analysis also revealed the presence of Pseudomonas and Acinetobacter as the most prevalent competing microbiota in enriched culture and only Acinetobacter on agar plates in the selective culture step.

Sign in / Sign up

Export Citation Format

Share Document